LOCUS       sequence-338670-       10458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  G418 resistant LexA driver vector with Mini-w+ CDS eye marker. 
            Enhancer grammar GB20 entry point for custom enhancers. 
            Cut-and-paste cloning can be used. Purple-white bacteria colony 
            screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pG418R-MCS-LexA-VP16-MiniWhite
FEATURES             Location/Qualifiers
     source          1..10458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    121..190
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        218..669
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     617..638
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     promoter        1618..1636
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1618..1635
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1647..1664)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      2472..2566
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    2573..2629
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(2691..2709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2872..3123,3124..3477)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     CDS             3529..3762
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDTADFEFEQMFTDALGIDEYGG"
     polyA_signal    3770..3904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3794..3813)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3848..3867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3992..4010)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4462..4533,4935..5208,5283..5937,5999..6314,6518..6649,
                     6720..7334)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     rep_origin      complement(8101..8689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8181..8200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9157..9948
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10003..10431
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10090..10109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10300..10321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgcagctgc ccgggtctag agtcgacgca tgcctcctga ctcgacgatt
       61 tacgggaaat gaagctctcc ggatcccagg gaggaattgg tcgacgatgt aggtcactgt
      121 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
      181 ctcacccatc tggtccatca tgatgcgctg tcaggagcta gaatcccaaa acaaactggt
      241 tattgtggta ggtcatttgt ttggcagaaa gaaaactcga gaaatttctc tggccgttat
      301 tcgttattct ctcttttctt tttgggtcgc tccctctctg cactaatgct ctctcactct
      361 gtcacacagt aaacggcata ctgctctcgt tggttcgaga gagcgcgcct cgaatgttcg
      421 cgaaaagagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      481 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      541 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      601 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
      661 gagaactctc aatactcgaa tccatgtggg agtttattct tgacacagat atttatgata
      721 taataactga gtaagcttaa cataaggagg aaaacaaaat gattgagcag gatggactgc
      781 acgctggctc gccagctgcc tgggtggagc gtctgttcgg ctacgattgg gcccagcaga
      841 ccatcggctg ctccgatgct gccgtgttcc gtctgtcggc ccagggacgc cccgtgctgt
      901 tcgtcaagac cgacctgagt ggagccctga acgagctcca ggatgaggca gctcgtctga
      961 gctggctggc cacaacagga gtgccgtgtg ctgccgtgct ggatgtggtg accgaagctg
     1021 gacgcgattg gctgctgctg ggcgaggtgc caggccagga cctgctgtcc agccatctgg
     1081 ccccagccga gaaggtgtcc attatggccg atgctatgcg tcgcctgcac accctggacc
     1141 cagccacctg cccctttgac caccaggcca agcaccgtat tgagcgtgcc cgaacccgta
     1201 tggaggctgg actggtggac caggatgacc tggatgagga gcaccagggc ctggctccag
     1261 ccgagctgtt tgcccgtctg aaggcccgta tgcccgatgg cgaggacctg gtggtgaccc
     1321 acggcgacgc ctgcctgccc aacattatgg tggagaacgg acgcttcagt ggcttcattg
     1381 attgtggacg cctgggcgtg gccgaccgtt accaggatat cgccctggcc acccgagata
     1441 tcgccgagga gctgggaggc gagtgggccg accgattcct ggtgctctac ggcattgcag
     1501 ccccagatag ccagcgtatt gccttctacc gtctgctgga cgagttcttc taagcttggc
     1561 aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttccgctg tcaggagatt
     1621 taggtgacac tatagacttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1681 ctgtcgggag agagaccaat ccatgtggga gtttattctt gacacagata tttatgatat
     1741 aataactgag taagcttaac ataaggagga aaacaaaatg gcgagcttgg ttaagaaaga
     1801 tatgtgtgtt aagatgacga tggaaggtac tgtgaacggt tatcacttta agtgcgttgg
     1861 cgagggtgaa ggcaagccgt tcgagggcac gcagaacatg cgcattcgtg tcaccgaggg
     1921 cggtccgctg ccttttgcat tcgacatcct ggccccgtgc tgtatgtacg gctctaagac
     1981 cttcattaaa cacgtgagcg gtatcccgga ttactttaaa gagtcctttc cagagggctt
     2041 cacttgggaa cgtacccaga tttttgagga cggtggtgtt ctgaccgcgc accaagacac
     2101 cagcctggaa ggtaattgcc tgatctataa agtgaaggtt ctgggtacca atttcccggc
     2161 gaatggtccg gtgatgcaaa agaaaaccgc gggttgggag ccgtgcgtcg agatgctgta
     2221 tccgcgtgac ggcgtcttgt gtggtcagag cttgatggcg ctgaagtgca ccgatggcaa
     2281 tcatctgacc agccacctgc gcacgacgta tcgtagccgt aaaccgagca acgccgttaa
     2341 catgccggag ttccattttg gtgaccatcg catcgaaatc ctgaaagctg agcagggcaa
     2401 attctacgaa caatacgaat cggctgtcgc acgttacagc gatgtgccgg aaaaagcgac
     2461 gtaatgagct tgactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     2521 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaattact tcaagcttgc
     2581 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattcg gtctcttccc
     2641 gcttgtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cctatagtga
     2701 gtcgtattat cccgagctcg cccggggatc gagcgcagcg gtataaaagg gcgcggggtg
     2761 gctgagagca tcagttgtga atgaatgttc gagccgagca gacgtgccgc tgccttcgtt
     2821 aatatccttt gaataagcca actttgaatc acaagacgca taccaaacca aatgaaggct
     2881 ctcacggccc gacaacagga agtttttgat ttgatacggg atcatatatc ccaaacgggt
     2941 atgcctccga cccgcgcaga gatagcacag cgactgggct ttcgatcgcc taacgccgcg
     3001 gaggagcact tgaaggcact ggcccgcaag ggtgtcattg aaatcgtgtc cggtgcgagc
     3061 cgcggaatcc ggctgttgca ggaggaggaa gagggcctgc cactggtggg acgcgtggcc
     3121 gctggcgagc cgctgctggc ccagcaacac atagagggac actatcaggt ggacccctcc
     3181 ttgtttaagc caaatgctga tttcctgttg cgggtgtcgg gaatgtccat gaaggacatc
     3241 ggtattatgg atggtgacct cctcgccgtc cataagacac aggatgtcag gaacggccag
     3301 gtagtcgttg ccaggataga cgatgaggtc actgtgaaac gcctcaagaa gcaaggcaat
     3361 aaggtcgagc tgctgccgga gaatagcgag ttcaagccga tcgtggtgga tctgcgacag
     3421 cagtccttta ctatcgaggg cttggccgtg ggtgtgatcc gcaacggaga ttggctgagt
     3481 ggcggaggtt ccggtggcgg cagcggcggg ggttcgggag gtggctcggc ccccccgacc
     3541 gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggccat ggcgcatgcc
     3601 gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc ggggccggga
     3661 tttacccccc acgactccgc cccctacggc gctctggata cggccgactt cgagtttgag
     3721 cagatgttta ccgatgccct tggaattgac gagtacggtg ggtaagcttg atccagacat
     3781 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3841 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3901 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3961 tttttcgctg tcaggaggaa ttctagtatg tatgtaagtt aataaaaccc atttttgcgg
     4021 aaagtagata aaaaaaacat tttttttttt tactgcactg gatatcattg aacttatctg
     4081 atcagtttta aatttacttc gatccaaggg tatttgatgt accaggttct ttcgattacc
     4141 tctcactcaa aatgacattc cactcaaagt cagcgctgtt tgcctccttc tctgtccaca
     4201 gaaatatcgc cgtcactttc gccgctgcgt ccgctatctc tttcgccacc gtttgtagcg
     4261 ttacgtagcg tcaatgtccg ccttcagttg cattttgtca gcggtttcgt gacgaagctc
     4321 caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct ctcgcttctt
     4381 atttttgttt gttttttgag tgattggggt ggtgattggt tttgggtggg taagcagggg
     4441 aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat tcgcggaggc
     4501 agcaaacacc catctgccga gcatctgaac aatgtgagta gtacatgtgc atacatctta
     4561 agttcacttg atctatagga actgcgattg caacatcaaa ttgtctgcgg cgtgagaact
     4621 gcgacccaca aaaatcccaa accgcaattg cacaaacaaa tagtgacacg aaacagatta
     4681 ttctggtagc tgttctcgct atataagaca atttttgaga tcatatcatg atcaagacat
     4741 ctaaaggcat tcattttcga ctatattctt ttttacaaaa aatataacaa ccagatattt
     4801 taagctgatc ctagatgcac aaaaaataaa taaaagtata aacctacttc gtaggatact
     4861 tcggggtact ttttgttcgg ggttagatga gcataacgct tgtagttgat atttgagatc
     4921 ccctatcatt gcagggtgac agcggagcgg cttcgcagag ctgcattaac cagggcttcg
     4981 ggcaggccaa aaactacggc acgctccggc cacccagtcc gccggaggac tccggttcag
     5041 ggagcggcca actagccgag aacctcacct atgcctggca caatatggac atctttgggg
     5101 cggtcaatca gccgggctcc ggatggcggc agctggtcaa ccggacacgc ggactattct
     5161 gcaacgagcg acacataccg gcgcccagga aacatttgct caagaacggt gagtttctat
     5221 tcgcagtcgg ctgatctgtg tgaaatctta ataaagggtc caattaccaa tttgaaactc
     5281 agtttgcggc gtggcctatc cgggcgaact tttggccgtg atgggcagtt ccggtgccgg
     5341 aaagacgacc ctgctgaatg cccttgcctt tcgatcgccg cagggcatcc aagtatcgcc
     5401 atccgggatg cgactgctca atggccaacc tgtggacgcc aaggagatgc aggccaggtg
     5461 cgcctatgtc cagcaggatg acctctttat cggctcccta acggccaggg aacacctgat
     5521 tttccaagcc atggtgcgga tgccacgaca tctgacctat cggcagcgag tggcccgcgt
     5581 ggatcaggtg atccaggagc tttcgctcag caaatgtcag cacacgatca tcggtgtgcc
     5641 cggcagggtg aaaggtctgt ccggcggaga aaggaagcgt ctggcattcg cctccgaggc
     5701 tctaaccgat ccgccgcttc tgatctgcga tgagcccacc tccggactgg actcctttac
     5761 cgcccacagc gtcgtccagg tgctgaagaa gctgtcgcag aagggcaaga ccgtcatcct
     5821 gaccattcat cagccgtctt ccgagctgtt tgagctcttt gacaagatcc ttctgatggc
     5881 cgagggcagg gtagctttct tgggcactcc cagcgaagcc gtcgacttct tttcctagtg
     5941 agttcgatgt gtttattaag ggtatctagt attacataac atctcaactc ctatccagcg
     6001 tgggtgccca gtgtcctacc aactacaatc cggcggactt ttacgtacag gtgttggccg
     6061 ttgtgcccgg acgggagatc gagtcccgtg atcggatcgc caagatatgc gacaattttg
     6121 ccattagcaa agtagcccgg gatatggagc agttgttggc caccaaaaat ctggagaagc
     6181 cactggagca gccggagaat gggtacacct acaaggccac ctggttcatg cagttccggg
     6241 cggtcctgtg gcgatcctgg ctgtcggtgc tcaaggaacc actcctcgta aaagtgcgac
     6301 ttattcagac aacggtgagt ggttccagtg gaaacaaatg atataacgct tacaattctt
     6361 ggaaacaaat tcgctagatt ttagatagaa ttgcctgatt ccacaccctt cttagttttt
     6421 ttcaatgaga tgtatagttt atagttttgc agaagataaa taaatttcat ttaactcgcg
     6481 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     6541 catctttttg ggccaacaac tcacgcaagt gggtgtgatg aatatcaacg gagccatctt
     6601 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcatgt
     6661 ttagaataca tttgcatttc aataatttac taactttcta atgaatcgat tcgatttagg
     6721 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     6781 acacatactt tctgggcaaa acgattgccg aattgccgct ttttctcaca gtgccactgg
     6841 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     6901 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     6961 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     7021 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     7081 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     7141 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     7201 aggtcatcct ggagacactt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     7261 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     7321 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     7381 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     7441 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     7501 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     7561 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     7621 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     7681 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     7741 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc ggctcaatag
     7801 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     7861 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     7921 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     7981 tgtgtagatg catcgcttga cacggttatc cacagaatca ggggataacg caggaaagaa
     8041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8341 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8401 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8461 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     8521 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8581 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8761 catgagatta tcaaaaagga tcttcaccta gatccttttg tgaccagcca ggacagaaat
     8821 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     8881 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     8941 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     9001 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     9061 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     9121 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     9181 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     9241 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     9301 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     9361 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     9421 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     9481 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     9541 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     9601 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     9661 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     9721 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtagct
     9781 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     9841 caggcttatc ttggacaaga agaagatcgt ttggcctcgc gcgcagatca gttggaagaa
     9901 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc actagaccaa
     9961 tgttacacat atatacttta gattgattta aaacttcatg ttacgcgccc tgtagcggcg
    10021 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    10081 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    10141 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    10201 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg
    10261 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    10321 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    10381 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    10441 tattaacgct tacaattt
//
