LOCUS       sequence-338716-        2978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Streptomyces codon-optimised mVenus-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Moore SJ, Lai HE, Chee SM, Toh M, Coode S, Chengan K, Capel P, Corre
            C, de Los Santos EL, Freemont PS
  TITLE     A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2021 Feb 19;10(2):402-411. doi: 
            10.1021/acssynbio.0c00581. Epub 2021 Jan 26.
  PUBMED    33497199
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00581"; journalName: "ACS Synth Biol"; date: 
            "2021-02-19- 19"; volume: "10"; issue: "2"; pages: "402-411"
COMMENT     Sequence Label: pTU1-A-SP44-mVenus-I
FEATURES             Location/Qualifiers
     source          1..2978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     39..57
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(61..104)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    107..126
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(282..303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(543..562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             966..983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      994..1021
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1054..1071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1225..1813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1305..1324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1984..2775,2776..2844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2607..2626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2845..2949)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
       61 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
      121 ttctagaacg tctcaatctc tattgttcac attcgaaccg tctctgcttt gacaacatgc
      181 tgtgcggtgt tgtaaagtct ggtgtagtac tttaacttta agaaggagat atacatatgg
      241 tgtccaaggg cgaggagctg ttcaccggcg tcgtcccgat cctggtcgag ctggacggcg
      301 acgtcaacgg ccacaagttc tccgtctccg gcgagggcga gggcgacgcc acctacggca
      361 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc ggtcccgtgg ccgaccctgg
      421 tcaccaccct gggctacggc ctgcagtgct tcgcccgcta cccggaccac atgaagcagc
      481 acgacttctt caagtccgcc atgccggagg gctacgtcca ggagcgcacc atcttcttca
      541 aggacgacgg caactacaag acccgcgccg aggtcaagtt cgagggcgac accctggtca
      601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg ggccacaagc
      661 tggagtacaa ctacaactcc cacaacgtct acatcaccgc cgacaagcag aagaacggca
      721 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtccag ctggccgacc
      781 actaccagca gaacaccccg atcggcgacg gcccggtcct gctgccggac aaccactacc
      841 tgtcctacca gtccaagctg tccaaggacc cgaacgagaa gcgcgaccac atggtcctgc
      901 tggagttcgt caccgccgcc ggcatcaccc tgggcatgga cgagctgtac aagagatcct
      961 cgagccacca ccaccaccac cactaatcac actggctcac cttcgggtgg gcctttctgc
     1021 gtttatatgt ttgccagaga cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt
     1081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2941 ggttccgcgc acatttcccc gaaaagtgcc acctgacg
//
