LOCUS       sequence-338745-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0181
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2524..3112
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2577..2597)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2995..3006)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3184..3417
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3495..3616
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3532..3551)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3586..3605
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3656..3791
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3811..3830)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3812..3830)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3840..3857)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3840..3856)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3849..3871)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3998..4453
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4085..4104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4295..4316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4479..4583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4584..4652,4653..5444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4802..4821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5615..6203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6104..6123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6357..6374
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6491..6512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6527..6532,6533..6550,6551..6557)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6565..6581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6570..6592
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6589..6605
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6589..6605
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6624..6644
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6626..6644
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6672..6898
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6899..7079
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7126..7251
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7744..7977
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8162..8206
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8437..8647
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8459..8655
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8506..8641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8687..9289
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8687..8706)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9183..9203
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9332..9449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gtctcttcgc gggtacaccc acagtgatat tggctaccgc agcctggcag tcggcgagga
     1261 catcgaggaa gtgaatgatg agaaacttac cgtgacctcg ctgatggcaa ggggaggaga
     1321 ggacgaggag aatacacggt ccaaacctga gtacggaaca gaagcggaaa acaacgttgg
     1381 cacggaaggg tccgtcccct cggacgacca agaccgtgag ggtggcggcg gccacgagcc
     1441 ggagcaacag caggaggagc cgcccctgac caagccggag caacagcagg aggagccgcc
     1501 cctgctcgag ctgaagcaag agcaggagga gccgccccag acgaccgtgg aggggccaca
     1561 gccggcggag gggccacaaa ccgctgaggg accacagccc ccagagagga aacgccgccg
     1621 ccgcaccgcg ttcacgcagt ttcagctgca ggagctagag aactttttcg atgaatctca
     1681 atatcccgac gttgtggcgc gagagagact tgcagcacgc ctgaatttga ctgaagacag
     1741 agtgcaggtt tggtttcaga acagaagagc caagtggaaa cgaaatcaga gggtgctaat
     1801 gttgagaaac actgctactg ctgacctggc ccaccctttg gacatgttct tgggtggggc
     1861 ctattatgct gctcctgctc tggatcctgc tttgtgtgtt catctggtgc cacaactacc
     1921 tagaccacct gtgctgcctg tgccacctat gccacccagg ccacccatgg tccctatgcc
     1981 acccaggcca cccatagcac ctatgccacc catggcgcct gtgccacccg gctcacgcat
     2041 ggcgcctgtg ccacccgggc cacgcatggc gcctgtgcca ccctggccgc ccatggcgcc
     2101 tgtgccaccc tggccgccca tggcgcctgt gccaaccggg ccgcccatgg cgcctgtgcc
     2161 acccgggccg cccatggcgc gtgtgccacc cgggccgccc atggcgcgtg tgccacccgg
     2221 gccgcccatg gcgcctctgc cacccgggcc gcccatggcg cctctgccac ccgggccgcc
     2281 catggcgcct ctgccacccg ggccgcccat ggcgcctctg ccacccaggt cacatgtgcc
     2341 tcacactggc ctggctcctg tacacatcac atgggcccct gtcatcaaca gttattatgc
     2401 atgtcccttt ttctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     2461 ttatatatct tgtggaaagg acgaggcagg gatacattac acatgtgcac gcgttaagtc
     2521 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2581 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2641 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2701 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2761 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2821 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     2881 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     2941 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3001 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3061 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     3121 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     3181 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3421 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     3481 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3541 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3601 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     3661 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3721 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3781 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     3841 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3901 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3961 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4021 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4081 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4141 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4201 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4261 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4321 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4381 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4441 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4501 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4561 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4621 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4681 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4741 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4801 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4861 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4921 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4981 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5041 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5101 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5161 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5221 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5281 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5341 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5401 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5461 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5521 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5581 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5641 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5701 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5761 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5821 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5881 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5941 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6001 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6061 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6121 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6181 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6241 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6301 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6361 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6421 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6481 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6541 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     6601 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     6661 tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
     6721 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     6781 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     6841 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
     6901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7081 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     7141 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     7201 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     7261 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     7321 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     7381 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     7441 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     7501 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     7561 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     7621 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     7681 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     7741 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     7801 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     7861 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     7921 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     7981 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     8041 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     8101 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     8161 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     8221 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     8281 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     8341 attatcgttt cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag
     8401 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8461 cgtctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8641 ctaggctttt gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa
     8701 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     8761 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     8821 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     8881 ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg
     8941 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     9001 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     9061 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     9121 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     9181 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     9241 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga
     9301 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     9361 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9421 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9481 tttggcgccg gctcgaggcc tgcaggtgca aagatggata aagttttaaa cagagaggaa
     9541 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaatt
//
