LOCUS       sequence-338747-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of bacteriophage-derived MS2 coat protein fused HIV-1 
            Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage 
            signal between MS2 and Gag-Pol. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, 
            Sun X, Yang H, Paludan SR, Cai Y
  TITLE     Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting 
            guide RNA prevents wet age-related macular degeneration in mice.
  JOURNAL   Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 
            10.1038/s41551-020-00656-y.
  PUBMED    33398131
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 
            10.1038/s41551-020-00656-y."
COMMENT     Sequence Label: pMDlg/pRRE-int-MS2M
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(205..222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(376..964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(456..475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1135..1926,1927..1995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1758..1777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1996..2100)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2170..2189)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        2291..2670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2671..2874
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2825..2845
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2871..2895
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3008..3483
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     regulatory      3561..3570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3567..5069
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             4862..7870
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    7558..7675
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             7913..8302
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    8317..8550
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    8781..9175
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 gttttacggc gagatggttt ctcctcgcct ggccactcag ccttagttgt ctctgttgtc
       61 ttatagaggt ctacttgaag aaggaaaaac agggggcatg gtttgactgt cctgtgagcc
      121 cttcttccct gcctccccca ctcacagtga cccggaatcc ctcgacatgg cagtctagca
      181 ctagtgcggc cgcagatctg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     2101 cacatttccc cgaaaagtgc cacctgacgt ggatcccctg agggggcccc catgggctag
     2161 aggatccggc ctcggcctct gcataaataa aaaaaattag tcagccatga gcttggccca
     2221 ttgcatacgt tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta
     2281 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     2341 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2401 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2461 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2521 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2581 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2641 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2701 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2761 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2821 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     2881 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2941 cgggaccgat ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa
     3001 cttcagggtg agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag
     3061 ttcatgtcat aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt
     3121 ttgcatttgt aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat
     3181 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
     3241 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
     3301 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
     3361 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
     3421 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
     3481 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag
     3541 atctgaattc gagatctgcc gccgccatgg gtgcgagagc gtcagtatta agcgggggag
     3601 aattagatcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     3661 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     3721 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3781 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3841 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3901 agaaaaaagc acagcaagca gcagctgaca caggacacag caatcaggtc agccaaaatt
     3961 accctatagt gcagaacatc caggggcaaa tggtacatca ggccatatca cctagaactt
     4021 taaatgcatg ggtaaaagta gtagaagaga aggctttcag cccagaagtg atacccatgt
     4081 tttcagcatt atcagaagga gccaccccac aagatttaaa caccatgcta aacacagtgg
     4141 ggggacatca agcagccatg caaatgttaa aagagaccat caatgaggaa gctgcagaat
     4201 gggatagagt gcatccagtg catgcagggc ctattgcacc aggccagatg agagaaccaa
     4261 ggggaagtga catagcagga actactagta cccttcagga acaaatagga tggatgacac
     4321 ataatccacc tatcccagta ggagaaatct ataaaagatg gataatcctg ggattaaata
     4381 aaatagtaag aatgtatagc cctaccagca ttctggacat aagacaagga ccaaaggaac
     4441 cctttagaga ctatgtagac cgattctata aaactctaag agccgagcaa gcttcacaag
     4501 aggtaaaaaa ttggatgaca gaaaccttgt tggtccaaaa tgcgaaccca gattgtaaga
     4561 ctattttaaa agcattggga ccaggagcga cactagaaga aatgatgaca gcatgtcagg
     4621 gagtgggggg acccggccat aaagcaagag ttttggctga agcaatgagc caagtaacaa
     4681 atccagctac cataatgata cagaaaggca attttaggaa ccaaagaaag actgttaagt
     4741 gtttcaattg tggcaaagaa gggcacatag ccaaaaattg cagggcccct aggaaaaagg
     4801 gctgttggaa atgtggaaag gaaggacacc aaatgaaaga ttgtactgag agacaggcta
     4861 attttttagg gaagatctgg ccttcccaca agggaaggcc agggaatttt cttcagagca
     4921 gaccagagcc aacagcccca ccagaagaga gcttcaggtt tggggaagag acaacaactc
     4981 cctctcagaa gcaggagccg atagacaagg aactgtatcc tttagcttcc ctcagatcac
     5041 tctttggcag cgacccctcg tcacaataaa gatagggggg caattaaagg aagctctatt
     5101 agatacagga gcagatgata cagtattaga agaaatgaat ttgccaggaa gatggaaacc
     5161 aaaaatgata gggggaattg gaggttttat caaagtaaga cagtatgatc agatactcat
     5221 agaaatctgc ggacataaag ctataggtac agtattagta ggacctacac ctgtcaacat
     5281 aattggaaga aatctgttga ctcagattgg ctgcacttta aattttccca ttagtcctat
     5341 tgagactgta ccagtaaaat taaagccagg aatggatggc ccaaaagtta aacaatggcc
     5401 attgacagaa gaaaaaataa aagcattagt agaaatttgt acagaaatgg aaaaggaagg
     5461 aaaaatttca aaaattgggc ctgaaaatcc atacaatact ccagtatttg ccataaagaa
     5521 aaaagacagt actaaatgga gaaaattagt agatttcaga gaacttaata agagaactca
     5581 agatttctgg gaagttcaat taggaatacc acatcctgca gggttaaaac agaaaaaatc
     5641 agtaacagta ctggatgtgg gcgatgcata tttttcagtt cccttagata aagacttcag
     5701 gaagtatact gcatttacca tacctagtat aaacaatgag acaccaggga ttagatatca
     5761 gtacaatgtg cttccacagg gatggaaagg atcaccagca atattccagt gtagcatgac
     5821 aaaaatctta gagcctttta gaaaacaaaa tccagacata gtcatctatc aatacatgga
     5881 tgatttgtat gtaggatctg acttagaaat agggcagcat agaacaaaaa tagaggaact
     5941 gagacaacat ctgttgaggt ggggatttac cacaccagac aaaaaacatc agaaagaacc
     6001 tccattcctt tggatgggtt atgaactcca tcctgataaa tggacagtac agcctatagt
     6061 gctgccagaa aaggacagct ggactgtcaa tgacatacag aaattagtgg gaaaattgaa
     6121 ttgggcaagt cagatttatg cagggattaa agtaaggcaa ttatgtaaac ttcttagggg
     6181 aaccaaagca ctaacagaag tagtaccact aacagaagaa gcagagctag aactggcaga
     6241 aaacagggag attctaaaag aaccggtaca tggagtgtat tatgacccat caaaagactt
     6301 aatagcagaa atacagaagc aggggcaagg ccaatggaca tatcaaattt atcaagagcc
     6361 atttaaaaat ctgaaaacag gaaagtatgc aagaatgaag ggtgcccaca ctaatgatgt
     6421 gaaacaatta acagaggcag tacaaaaaat agccacagaa agcatagtaa tatggggaaa
     6481 gactcctaaa tttaaattac ccatacaaaa ggaaacatgg gaagcatggt ggacagagta
     6541 ttggcaagcc acctggattc ctgagtggga gtttgtcaat acccctccct tagtgaagtt
     6601 atggtaccag ttagagaaag aacccataat aggagcagaa actttctatg tagatggggc
     6661 agccaatagg gaaactaaat taggaaaagc aggatatgta actgacagag gaagacaaaa
     6721 agttgtcccc ctaacggaca caacaaatca gaagactgag ttacaagcaa ttcatctagc
     6781 tttgcaggat tcgggattag aagtaaacat agtgacagac tcacaatatg cattgggaat
     6841 cattcaagca caaccagata agagtgaatc agagttagtc agtcaaataa tagagcagtt
     6901 aataaaaaag gaaaaagtct acctggcatg ggtaccagca cacaaaggaa ttggaggaaa
     6961 tgaacaagta gataaattgg tcagtgctgg aatcaggaaa gtactatttt tagatggaat
     7021 agataaggcc caagaagaac atgagaaata tcacagtaat tggagagcaa tggctagtga
     7081 ttttaaccta ccacctgtag tagcaaaaga aatagtagcc agctgtgata aatgtcagct
     7141 aaaaggggaa gccatgcatg gacaagtaga ctgtagccca ggaatatggc agctagattg
     7201 tacacattta gaaggaaaag ttatcttggt agcagttcat gtagccagtg gatatataga
     7261 agcagaagta attccagcag agacagggca agaaacagca tacttcctct taaaattagc
     7321 aggaagatgg ccagtaaaaa cagtacatac agacaatggc agcaatttca ccagtactac
     7381 agttaaggcc gcctgttggt gggcggggat caagcaggaa tttggcattc cctacaatcc
     7441 ccaaagtcaa ggagtaatag aatctatgaa taaagaatta aagaaaatta taggacaggt
     7501 aagagatcag gctgaacatc ttaagacagc agtacaaatg gcagtattca tccacaattt
     7561 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     7621 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     7681 ttattacagg gacagcagag atccagtttg gaaaggacca gcaaagctcc tctggaaagg
     7741 tgaaggggca gtagtaatac aagataatag tgacataaaa gtagtgccaa gaagaaaagc
     7801 aaagatcatc agggattatg gaaaacagat ggcaggtgat gattgtgtgg caagtagaca
     7861 ggatgaggat ggaggatccc aagtcgacag ccagaactat ccgattgtgc aggcctctaa
     7921 ttttactcaa tttgtgcttg tcgataatgg ggggacggga gatgtgaccg ttgcccctag
     7981 caatttcgca aatggcgttg cagaatggat ctctagcaac agcagaagcc aagcgtacaa
     8041 agtaacgtgt tccgttcgcc aaagctccgc ccaaaaacgg aagtatacaa taaaggttga
     8101 ggtgccgaaa gtagccactc aaacagttgg tggggtagaa ttgcccgtag cggcatggcg
     8161 gtcatatctc aatatggaac tcactatccc aatcttcgcc acgaatagcg attgtgagct
     8221 gatagttaag gctatgcaag gtcttctcaa agatggaaac cctattccat ctgctatcgc
     8281 cgccaacagc ggcatatact agtccggagc ggccgcagga gctttgttcc ttgggttctt
     8341 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8401 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8461 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8521 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8581 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8641 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttccgcgg
     8701 aattcacccc accagtgcag gctgcctatc agaaagtggt ggctggtgtg gctaatgccc
     8761 tggcccacaa gtatcactaa gctcgctttc ttgctgtcca atttctatta aaggttcctt
     8821 tgttccctaa gtccaactac taaactgggg gatattatga agggccttga gcatctggat
     8881 tctgcctaat aaaaaacatt tattttcatt gcaatgatgt atttaaatta tttctgaata
     8941 ttttactaaa aagggaatgt gggaggtcag tgcatttaaa acataaagaa atgaagagct
     9001 agttcaaacc ttgggaaaat acactatatc ttaaactcca tgaaagaagg tgaggctgca
     9061 aacagctaat gcacattggc aacagcccct gatgcctatg ccttattcat ccctcagaaa
     9121 aggattcaag tagaggcttg atttggaggt taaagttttg ctatgctgta ttttacatta
     9181 cttattgttt tagctgtcct catgaatgtc ttttcactac ccatttgctt atcctgcatc
     9241 tctcagcctt gactccactc agttctcttg cttagagata ccacctttcc cctgaagtgt
     9301 tccttccat
//
