LOCUS       sequence-338748-       10216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1282
FEATURES             Location/Qualifiers
     source          1..10216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3154..3742
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3207..3227)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3625..3636)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3814..4047
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4125..4246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4162..4181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4216..4235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4286..4421
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4441..4460)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4442..4460)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4470..4487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4470..4486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4479..4501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4628..5083
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4715..4734)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4925..4946
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5109..5213
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5214..5282,5283..6074)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5432..5451)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6245..6833
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6734..6753
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6987..7004
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7121..7142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7157..7162,7163..7180,7181..7187)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7195..7211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7200..7222
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7219..7235
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7219..7235
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7254..7274
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7256..7274
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7302..7528
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7529..7709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7756..7881
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8374..8607
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8792..8836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9067..9277
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9089..9285
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9136..9271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9317..9919
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9317..9336)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9813..9833
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9962..10079
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 gaggcgcaag caggcgaaac cccagcacat caactcggag gaggaccagg gcgagcagca
     1261 gccgcagcag cagaccccgg agtttgcaga tgcggcccca gcggcgcccg cggcggggga
     1321 gctgggtgct ccagtgaacc acccagggaa tgacgaggtg gcgagtgagg atgaagccac
     1381 agtaaagcgg cttcgtcggg aggagacgca cgtctgtgag aaatgctgtg cggagttctt
     1441 cagcatctct gagttcctgg aacataagaa aaattgcact aaaaatccac ctgtcctcat
     1501 catgaatgac agcgaggggc ctgtgccttc agaagacttc tccggagctg tactgagcca
     1561 ccagcccacc agtcccggca gtaaggactg tcacagggag aatggcggca gctcagagga
     1621 catgaaggag aagccggatg cggagtctgt ggtgtaccta aagacagaga cagccctgcc
     1681 acccaccccc caggacataa gctatttagc caaaggcaaa gtggccaaca ctaatgtgac
     1741 cttgcaggca ctacggggca ccaaggtggc ggtgaatcag cggagcgcgg atgcactccc
     1801 tgcccccgtg cctggtgcca acagcatccc gtgggtcctc gagcagatct tgtgtctgca
     1861 gcagcagcag ctacagcaga tccagctcac cgagcagatc cgcatccagg tgaacatgtg
     1921 ggcctcccac gccctccact caagcggggc aggggccgac actctgaaga ccttgggcag
     1981 ccacatgtct cagcaggttt ctgcagctgt ggctttgctc agccagaaag ctggaagcca
     2041 aggtctgtct ctggatgcct tgaaacaagc caagctacct cacgccaaca tcccttctgc
     2101 caccagctcc ctgtccccag ggctggcacc cttcactctg aagccggatg ggacccgggt
     2161 gctcccgaac gtcatgtccc gcctcccgag cgctttgctt cctcaggccc cgggctcggt
     2221 gctcttccag agccctttct ccactgtggc gctagacaca tccaagaaag ggaaggggaa
     2281 gccaccgaac atctccgcgg tggatgtcaa acccaaagac gaggcggccc tctacaagca
     2341 caagtgtcgg agcagtctcc cttccacgtt tatccgagcc ccgccgacct atgtcaaggt
     2401 tgaagttcct ggcacatttg tgggaccctc gacattgtcc ccagggatga cccctttgtt
     2461 agcagcccag ccacgccgac aggccaagca acatggctgc acacggtgtg ggaagaactt
     2521 ctcgtctgct agcgctcttc agatccacga gcggactcac actggagaga agccttttgt
     2581 gtgcaacatt tgtgggcgag cttttaccac caaaggcaac ttaaaggttc actacatgac
     2641 acacggggcg aacaataact cagcccgccg tggaaggaag ttggccatcg agaacaccat
     2701 ggctctgtta ggtacggacg gaaaaagagt ctcagaaatc tttcccaagg aaatcctggc
     2761 cccttcagtg aatgtggacc ctgttgtgtg gaaccagtac accagcatgc tcaatggcgg
     2821 tctggccgtg aagaccaatg agatctctgt gatccagagt gggggggttc ctaccctccc
     2881 ggtttccttg ggggccacct ccgttgtgaa taacgccact gtctccaaga tggatggctc
     2941 ccagtcgggt atcagtgcag atgtggaaaa accaagtgct actgacggcg ttcccaaaca
     3001 ccagtttcct cacttcctgg aagaaaacaa gattgcggtc agctagtaat gaactagtcc
     3061 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgcctc
     3121 acttattccg ggtaaagaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     3181 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3241 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3301 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3361 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3421 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3481 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3541 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3601 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3661 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3721 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3781 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3841 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4021 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     4081 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4141 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4201 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4261 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4321 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4381 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4441 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4501 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4561 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4621 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4681 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4741 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4801 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4861 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4921 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4981 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5041 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5101 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5161 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5221 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5281 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5341 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5401 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5461 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5521 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5581 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5641 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5701 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5761 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5821 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5881 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5941 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6001 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6061 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6121 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6181 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6241 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6301 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6361 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6421 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6481 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6541 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6601 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6661 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6721 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6781 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6841 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6901 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6961 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7021 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7081 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7141 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7201 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7261 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7321 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7381 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7441 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7501 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7561 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7621 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7681 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7741 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7801 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7861 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7921 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7981 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     8041 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     8101 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     8161 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8221 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8281 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8341 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8401 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8461 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8521 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8581 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8641 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8701 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8761 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8821 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8881 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8941 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     9001 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     9061 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     9121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     9301 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9361 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9421 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9481 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9541 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9601 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9661 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9721 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9781 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9841 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9901 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9961 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
    10021 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
    10081 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
    10141 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
    10201 gaaaggagtg ggaatt
//
