LOCUS       sequence-338758-       15506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W 
            mutant signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar 
            N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, 
            Hecht JT
  TITLE     Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic 
            cleft lip and palate.
  JOURNAL   Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 
            10.1093/hmg/ddac037.
  PUBMED    35147171
REFERENCE   2  (bases 1 to 15506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2022-07-21- 21"; volume: "31"; issue: "14"; 
            pages: "2348-2357"
COMMENT     Sequence Label: pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter
FEATURES             Location/Qualifiers
     source          1..15506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3964..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4240..4262)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4848..6637
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(6943..8868)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(9303..9321)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9389..9493
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9494..9562,9563..10354)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9712..9731)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10525..11113
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11014..11033
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    11875..11923
                     /note="synthetic polyadenylation signal"
     misc_feature    11937..12028
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     11977..11996
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        12207..12238
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(12279..12281,12282..12284,12285..12995)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12279..12281,12282..12284,12285..12995)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12324..12345)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12585..12604)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12932..12953
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    13182..13303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13531..13676
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13716..14741
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             13716..14741
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(14762..14781)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14806..14854
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cgcctctggc cagctttcat taccctctat ccttccaaat gaaaatgaaa
      781 aggttgtgca gctgaattca tccttttctc tgagatgctt tggggagagt gaagtgagct
      841 ggcagtaccc catgtctgaa gaagagagct ccgatgtgga aatcagaaat gaagaaaaca
      901 acagcggcct ttttgtgacg gtcttggaag tgagcagtgc ctcggcggcc cacacagggt
      961 tgtacacttg ctattacaac cacactcaga cagaagagaa tgagcttgaa ggcaggcaca
     1021 tttacatcta tgtgccagac ccagatgtag cctttgtacc tctaggaatg acggattatt
     1081 tagtcatcgt ggaggatgat gattctgcca ttataccttg tcgcacaact gatcccgaga
     1141 ctcctgtaac cttacacaac agtgaggggg tggtacctgc ctcctatgac agcagacagg
     1201 gctttaatgg gaccttcact gtagggccct atatctgtga ggccaccgtc aaaggaaaga
     1261 agttccagac catcccattt aatgtttatg ctttaaaagc aacatcagag ctggatctag
     1321 aaatggaagc tcttaaaacc gtgtataagt caggggaaac gattgtggtc acctgtgctg
     1381 tttttaacaa tgaggtggtt gaccttcaat ggacttaccc tggagaagtg aaaggcaaag
     1441 gcatcacaat gctggaagaa atcaaagtcc catccatcaa attggtgtac actttgacgg
     1501 tccccgaggc cacggtgaaa gacagtggag attacgaatg tgctgcccgc caggctacca
     1561 gggaggtcaa agaaatgaag aaagtcacta tttctgtcca tgagaaaggt ttcattgaaa
     1621 tcaaacccac cttcagccag ttggaagctg tcaacctgca tgaagtcaaa cattttgttg
     1681 tagaggtgcg ggcctaccca cctcccagga tatcctggct gaaaaacaat ctgactctga
     1741 ttgaaaatct cactgagatc accactgatg tggaaaagat tcaggaaata aggtatcgaa
     1801 gcaaattaaa gctgatccgt gctaaggaag aagacagtgg ccattatact attgtagctc
     1861 aaaatgaaga tgctgtgaag agctatactt ttgaactgtt aactcaagtt ccttcatcca
     1921 ttctggactt ggtcgatgat caccatggct caactggggg acagaccgta cggtgcacag
     1981 ctgaaggcac gccgcttcct gatattgagt ggatgatatg caaagatatt aagaaatgta
     2041 ataatgaaac ttcctggact attttggcca acaatgtctc aaacatcatc acggagatcc
     2101 actcccgaga caggagtacc gtggagggcc gtgtgacttt cgccaaagtg gaggagacca
     2161 tcgccgtgcg atgcctggct aagaatctcc ttggagctga gaaccgagag ctgaagctgg
     2221 tggctcccac cctgcgttct gaactcacgg tggctgctgc agtcctggtg ctgttggtga
     2281 ttgtgatcat ctcacttatt gtcctggttg tcatttggaa acagaaaccg aggtatgaaa
     2341 ttcgctggag ggtcattgaa tcaatcagcc cggatggaca tgaatatatt tatgtggacc
     2401 cgatgcagct gccttatgac tcaagatggg agtttccaag agatggacta gtgcttggtc
     2461 gggtcttggg gtctggagcg tttgggaagg tggttgaagg aacagcctat ggattaagcc
     2521 ggtcccaacc tgtcatgaaa gttgcagtga agatgctaaa acccacggcc agatccagtg
     2581 aaaaacaagc tctcatgtct gaactgaaga taatgactca cctggggcca catttgaaca
     2641 ttgtaaactt gctgggagcc tgcaccaagt caggccccat ttacatcatc acagagtatt
     2701 gcttctatgg agatttggtc aactatttgc ataagaatag ggatagcttc ctgagccacc
     2761 acccagagaa gccaaagaaa gagctggata tctttggatt gaaccctgct gatgaaagca
     2821 cacggagcta tgttatttta tcttttgaaa acaatggtga ctacatggac atgaagcagg
     2881 ctgatactac acagtatgtc cccatgctag aaaggaaaga ggtttctaaa tattccgaca
     2941 tccagagatc actctatgat cgtccagcct catataagaa gaaatctatg ttagactcag
     3001 aagtcaaaaa cctcctttca gatgataact cagaaggcct tactttattg gatttgttga
     3061 gcttcaccta tcaagttgcc cgaggaatgg agtttttggc ttcaaaaaat tgtgtccacc
     3121 gtgatctggc tgctcgcaac gtcctcctgg cacaaggaaa aattgtgaag atctgtgact
     3181 ttggcctggc cagagacatc atgcatgatt cgaactatgt gtcgaaaggc agtacctttc
     3241 tgcccgtgaa gtggatggct cctgagagca tctttgacaa cctctacacc acactgagtg
     3301 atgtctggtc ttatggcatt ctgctctggg agatcttttc ccttggtggc accccttacc
     3361 ccggcatgat ggtggattct actttctaca ataagatcaa gagtgggtac tggatggcca
     3421 agcctgacca cgctaccagt gaagtctacg agatcatggt gaaatgctgg aacagtgagc
     3481 cggagaagag accctccttt taccacctga gtgagattgt ggagaatctg ctgcctggac
     3541 aatataaaaa gagttatgaa aaaattcacc tggacttcct gaagagtgac catcctgctg
     3601 tggcacgcat gcgtgtggac tcagacaatg catacattgg tgtcacctac aaaaacgagg
     3661 aagacaagct gaaggactgg gagggtggtc tggatgagca gagactgagc gctgacagtg
     3721 gctacatcat tcctctgcct gacattgacc ctgtccctga ggaggaggac ctgggcaaga
     3781 ggaacagaca cagctcgcag acctctgaag agagtgccat tgagacgggt tccagcagtt
     3841 ccaccttcat caagagagag gacgagacca ttgaagacat cgacatgatg gacgacatcg
     3901 gcatagactc ttcagacctg gtggaagaca gcttcctgta atgactcgag gccggcaagg
     3961 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4081 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4141 gaggtgggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     4201 atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4261 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4321 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc ggtcgactct agagccccgc
     4381 cgccggacga actaaacctg actacggcat ctctgcccct tcttcgctgg tacgaggagc
     4441 gcttttgttt tgtattcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     4501 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     4561 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     4621 ctggagcaga ctttgcatgc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     4681 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     4741 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     4801 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     4861 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     4921 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     4981 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     5041 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     5101 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     5161 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     5221 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     5281 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     5341 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     5401 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     5461 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     5521 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     5581 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     5641 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     5701 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     5761 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     5821 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     5881 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     5941 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     6001 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     6061 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     6121 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     6181 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     6241 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     6301 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     6361 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     6421 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     6481 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatgc atatacagtc
     6541 agcatatgat acccagtagt agagtgggag tgctatcctt tgcatatgcc gccacctccc
     6601 aagggggcgt gaattttcgc tgcttgtcct tttcctgcat gctggttgct cccattctta
     6661 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
     6721 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
     6781 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
     6841 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
     6901 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
     6961 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
     7021 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
     7081 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
     7141 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
     7201 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
     7261 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
     7321 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
     7381 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
     7441 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
     7501 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
     7561 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
     7621 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
     7681 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
     7741 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
     7801 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
     7861 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
     7921 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     7981 tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     8041 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
     8101 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     8161 tcctgcccct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
     8221 tcctgctcct gcccctcctg cccctcctgc ccctcctgct cctgcccctc ctcctgctcc
     8281 tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc
     8341 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
     8401 ccctcctgcc cctcctcctg ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     8461 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     8521 tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
     8581 tcccgctcct gctcctgctc ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg
     8641 acgtttttgg ggtctccgga caccatctct atgtcttggc cctgatcctg agccgcccgg
     8701 ggctcctggt cttccgcctc ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc
     8761 accccctctt ctttgaggtc cactgccgcc ggagccttct ggtccagatg tgtctccctt
     8821 ctctcctagg ccatttccag gtcctgtacc tggcccctcg tcagacatga ttcacactaa
     8881 aagagatcaa tagacatctt tattagacga cgctcagtga atacagggag tgcagactcc
     8941 tgccccctcc aacagccccc ccaccctcat ccccttcatg gtcgctgtca gacagatcca
     9001 ggtctgaaaa ttccccatcc tccgaaccat cctcgtcctc atcaccaatt actcgcagcc
     9061 cggaaaactc ccgctgaaca tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa
     9121 attcctcgtc cccctttttg ctggacggta gggatgggga ttctcgggac ccctcctctt
     9181 cctcttcaag gtcaccagac agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg
     9241 cctgtgagga tcagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa
     9301 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     9361 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9421 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9481 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9541 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9601 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9661 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9721 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     9781 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9841 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9901 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9961 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    10021 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    10081 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10141 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10201 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10261 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10321 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10381 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10441 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10501 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10561 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10621 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    10681 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10741 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10801 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10861 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10921 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10981 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11041 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11101 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctgcgc
    11161 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
    11221 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
    11281 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
    11341 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
    11401 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc
    11461 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
    11521 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
    11581 ggaaggccat ccagcctcgc gtcgcaagga tcttgccgct attgagatcc agttcgatat
    11641 agcccactct tgcacccagt tgatcttcag catcttttac tttcaccagc gtttcggggt
    11701 gtgcaaaaac aggcaagcaa aatgccgcaa agaagggaat gagtgcgaca cgaaaatgtt
    11761 ggatgctcat actcgtcctt tttcaatatt attgaagcat ttatcagggt tactagtacg
    11821 tctctcaagg ataagtaagt aatattaagg tacgggaggt attggacagg ccgcaataaa
    11881 atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat agtactaaca
    11941 tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc tgtccccagt
    12001 gcaagtgcag gtgccagaac atttctctgg cctaactggc cggtacctga gctcactagc
    12061 aggatgtcca tattaggaca tctaggatgt ccatattagg acatctagga tgtccatatt
    12121 aggacatcta ggatgtccat attaggacat ctaggatgtc catattagga catctagatc
    12181 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
    12241 tggcaatccg gtactgttgg taaagccacc ggcgcgccat ggtgagcaag ggcgaggagc
    12301 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
    12361 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
    12421 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
    12481 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
    12541 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
    12601 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
    12661 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
    12721 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
    12781 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
    12841 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
    12901 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
    12961 ccgggatcac tctcggcatg gacgagctgt acaagagtca tggttttccc ccggaagtcg
    13021 aagagcagga cgacgggacg ctgccaatgt catgcgcaca agagtctggt atggatcgcc
    13081 acccggctgc gtgtgccagc gcgagaatca atgtataatg aggcgcgccc taaggccgcg
    13141 actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt
    13201 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
    13261 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
    13321 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
    13381 ctacaaatgt ggtaaaatcg ataagcgaac gccagcaaga cgtagcccag cgcgtcggcc
    13441 gccatgccct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
    13501 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
    13561 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat
    13621 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
    13681 gtcaacagcg tgccgcagat cccgggcaat gagatatgaa aaagcctgaa ctcaccgcga
    13741 cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct
    13801 cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc
    13861 gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat
    13921 cggccgcgct cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct
    13981 attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc
    14041 ccgctgttct gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc
    14101 agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg
    14161 atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca
    14221 ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc
    14281 ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg
    14341 gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg
    14401 tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact
    14461 tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca
    14521 ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg
    14581 cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa
    14641 tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg
    14701 gaaaccgacg ccccagcact cgtccgaggg caaaggaata ggggagatgg gggaggctaa
    14761 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
    14821 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    14881 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    14941 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    15001 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
    15061 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtctg gtccacgact
    15121 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
    15181 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
    15241 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
    15301 agatccgggc aacgttgttg cattgctgca ggcgcagaac tggtaggtat ggaagatcta
    15361 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
    15421 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
    15481 ctcatgtcca atatgaccgc catgtt
//
