LOCUS       sequence-338760-       10594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0013
FEATURES             Location/Qualifiers
     source          1..10594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3532..4120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3585..3605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4003..4014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4192..4425
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4503..4624
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4540..4559)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4594..4613
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4664..4799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4819..4838)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4820..4838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4848..4865)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4848..4864)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4857..4879)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5006..5461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5093..5112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5303..5324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5487..5591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5592..5660,5661..6452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5810..5829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6623..7211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7112..7131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7365..7382
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7499..7520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7535..7540,7541..7558,7559..7565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7573..7589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7578..7600
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7597..7613
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7597..7613
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7632..7652
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7634..7652
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7680..7906
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7907..8087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8134..8259
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8752..8985
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9170..9214
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9445..9655
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9467..9663
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9514..9649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9695..10297
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9695..9714)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10191..10211
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10340..10457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 ctgctgtagt cacaggagct gtagagagga ccccggtaca tctgaaagcc gggaaatgga
     1261 cccagtggcc tttgaggatg tggctgtgaa cttcacccag gaagagtgga cattgctgga
     1321 tatttcccag aagaatctct tcagggaagt gatgctggaa actttcagga acctgacctc
     1381 tataggaaaa aaatggagtg accagaacat tgaatatgag taccaaaacc ccagaagaag
     1441 cttcaggagt ctcatagaag agaaagtcaa tgaaattaaa gaagacagtc attgtggaga
     1501 aacttttacc caggttccag atgacagact gaacttccag gagaagaaag cttctcctga
     1561 agtaaaatca tgtgacagct ttgtgtgtgc agaagttggc ataggtaact catcttttaa
     1621 tatgagcatc agaggtgaca ctggacacaa ggcatatgag tatcaggaat atggaccaaa
     1681 gccatataag tgtcaacaac ctaaaaataa gaaagccttc aggtatcgcc catccattag
     1741 aacacaagaa agggatcaca ctggagagaa accctatgct tgtaaagtct gtggaaaaac
     1801 ctttattttc cattcaagca ttcgaagaca catggtaatg cacagtgggg atggaactta
     1861 taaatgtaaa ttttgtggga aagccttcca ttctttcagt ttatatctta tccatgaaag
     1921 aactcacact ggagagaaac catatgaatg taaacaatgt ggtaaatcct ttacttattc
     1981 tgctaccctt caaatacatg aaagaactca cactggggag aagccctatg aatgtagcaa
     2041 atgtgataaa gcatttcata gttctagttc ctatcataga catgaaagaa gtcacatggg
     2101 agagaagcct tatcaatgca aagaatgtgg aaaagcattt gcatatacca gttctcttcg
     2161 tagacatgaa aggacccact ctgggaaaaa accgtatgaa tgtaagcaat atggggaagg
     2221 cttatcctat cttataagtt ttcaaacaca cataagaatg aactctggag aaagacctta
     2281 taaatgtaag atatgtggga aaggctttta ttctgccaag tcatttcaaa cacatgaaaa
     2341 aactcacact ggagagaaac gctataaatg caagcaatgt ggtaaagcct tcaatctttc
     2401 cagttccttt cgatatcatg aaaggattca cactggagag aaaccctatg agtgtaagca
     2461 gtgtgggaaa gccttcagat ctgcctcaca gcttcgagtg cacggtggga ctcacactgg
     2521 agagaaaccc tatgaatgta aggaatgtgg gaaagccttc agatctacct cacaccttcg
     2581 agtgcatggt aggactcata ctggagagaa accctatgaa tgtaaggaat gtgggaaagc
     2641 cttcagatat gtgaagcacc ttcaaattca tgaaaggaca gaaaaacaca taagaatgcc
     2701 ctctggagaa agaccttata aatgtagtat atgtgagaaa ggcttttatt ctgccaagtc
     2761 atttcaaaca catgaaaaaa ctcacactgg agagaaaccc tatgaatgca accaatgtgg
     2821 taaagccttc agatgttgca attcccttcg atatcatgaa aggactcaca ctggagagaa
     2881 accctatgag tgtaagcaat gtgggaaagc cttcagatct gcctcacacc ttcgaatgca
     2941 tgaaaggact cacactggag agaaacccta tgagtgtaag caatgtggga aagccttcag
     3001 ttgtgcctca aaccttcgaa agcatggtag gactcacact ggagagaaac cctatgagtg
     3061 taagcaatgt gggaaagcct tcagatctgc ctcaaacctt cagatgcatg aaaggactca
     3121 cactggagag aaaccctatg aatgtaagga atgcgaaaaa gcattctgta aattctcttc
     3181 ttttcaaata catgaaagga agcacagagg agagaagccc tatgaatgta agcattgtgg
     3241 gaatggattc acatctgcca agattcttca aatacatgca agaacacaca ttggagagaa
     3301 acactatgaa tgtaaggaat gcggaaaagc attcaattat ttttcttcct tgcatataca
     3361 cgcaaggact catatgggag agaagccata tgaatgtaag gattgtggga aagcattcag
     3421 ctagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     3481 tggaaaggac gatcaagcga tgccgacgaa tcatgcacgc gttaagtcga caatcaacct
     3541 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3601 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3661 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3721 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3781 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3841 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3901 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3961 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4021 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4081 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4141 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4201 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4261 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4321 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4381 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     4441 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4501 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4561 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4621 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4801 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4861 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4921 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4981 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5041 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5101 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5161 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5221 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5281 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5341 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5401 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5461 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5521 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5581 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5641 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5701 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5761 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5821 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5881 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5941 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6001 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6061 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6121 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6181 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6241 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6301 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6361 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6421 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6481 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6541 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6601 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6661 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6721 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6781 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6841 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6901 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6961 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7021 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7081 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7141 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7201 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7261 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7321 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7381 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7441 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7501 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7561 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7621 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7681 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7741 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7801 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7861 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7921 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7981 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8041 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8101 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8161 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8221 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8281 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8341 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8401 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8461 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8521 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8581 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8641 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8701 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8761 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8821 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8881 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8941 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9001 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9061 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9121 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9181 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9241 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9301 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9361 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     9421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     9481 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9661 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     9721 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9781 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9841 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9901 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9961 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    10021 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    10081 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    10141 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    10201 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    10261 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
    10321 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10381 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10441 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10501 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
    10561 atggaccttc taggtcttga aaggagtggg aatt
//
