LOCUS       sequence-338763-       10096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1239
FEATURES             Location/Qualifiers
     source          1..10096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3034..3622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3087..3107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3505..3516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3694..3927
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4005..4126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4042..4061)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4096..4115
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4166..4301
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4321..4340)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4322..4340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4350..4367)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4350..4366)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4359..4381)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4508..4963
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4595..4614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4805..4826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4989..5093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5094..5162,5163..5954)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5312..5331)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6125..6713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6614..6633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6867..6884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7001..7022
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7037..7042,7043..7060,7061..7067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7075..7091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7080..7102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7099..7115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7099..7115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7134..7154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7136..7154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7182..7408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7409..7589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7636..7761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8254..8487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8672..8716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8947..9157
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8969..9165
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9016..9151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9197..9799
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9197..9216)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9693..9713
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9842..9959
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 cgacttgaaa tatggagtgt atcctctcaa ggaagcaagt ggatgccctg gggctgagag
     1261 gaatcttcta gtttactctt attttgaaaa ggagacattg acatttaggg atgtggccat
     1321 agaattctct ctggaggagt gggaatgcct gaaccctgct cagcagaatt tatatatgaa
     1381 tgtgatgtta gaaaactaca aaaacctggt cttcttgggt gttgctgtct ctaagcaaga
     1441 cccagtcacc tgtctggagc aagaaaaaga gccctggaat atgaagagac atgagatggt
     1501 ggatgaaccc ccagctatgt gttcttattt taccaaagac ctttggccag agcaagacat
     1561 aaaagattct tttcaacaag taatactgag aagatatggc aaatgtgaac atgagaattt
     1621 acagttaaga aaaggctccg caagtgtaga tgagtataag gtgcacaaag aaggttataa
     1681 tgagctaaac cagtgtttga caactaccca gagcaaaata tttccatgtg ataaatatgt
     1741 gaaagtcttt cataaatttt taaatgcaaa tagacataag acaagacata ctggaaagaa
     1801 acctttcaaa tgtaaaaaat gtggcaaatc attttgcatg cttttacacc taagtcaaca
     1861 taaaagaatt catattagag agaattctta ccaatgtgaa gaatgtggca aagcttttaa
     1921 atggttctca acccttacta gacacaagag aattcatact ggagagaaac ccttcaaatg
     1981 tgaagaatgt ggcaaagctt ttaagcagtc ctcaaccctt actacacata agataattca
     2041 tactggggag aaaccatata gatgtgaaga atgtggcaaa gccttcaacc ggtcctcaca
     2101 ccttactaca cataagataa ttcatactgg agagaagccc tacaaatgtg aagaatgtgg
     2161 caaagctttt aaccagtctt caacccttag tacacataag ttcattcatg ctggagagaa
     2221 accctacaaa tgtgaggaat gtgacaaagc ttttaatcga ttctcatacc ttactaaaca
     2281 taagataatt catactggag aaaaatctta caaatgtgaa gaatgtggca aaggctttaa
     2341 ttggtcctca acccttacta aacataaaag aattcatact ggagagaaac cctacaaatg
     2401 tgaagtgtgt ggcaaagcct ttaatgagtc ctcaaacctt actacacata agatgattca
     2461 tactggagag aaaccctaca aatgtgaaga atgtggcaaa gcttttaacc ggtccccaca
     2521 acttactgca cataagataa ttcatactgg agagaaacct tacaaatgtg aagaatgtgg
     2581 caaagctttt agccagtcat caatccttac tacacataag agaattcaca ctggagagaa
     2641 accctacaaa tgtgaagaat gtggcaaagc ttttaaccga tcctcaaatc ttactaaaca
     2701 taagataatt catacaggag agaaatctta caaatgtgaa gaatgtggta aagcctttaa
     2761 ccaatcctca actcttacta aacataggaa aattcatact agacagaaac cctacaactg
     2821 tgaagaatgt gacaatacat ttaaccagtc ctcaaacctt attaaacaaa ataattcata
     2881 ctggagagaa actctacaaa tgtcaagaat gtgggaaagc ctttagtaat gaactagtcc
     2941 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgacgaatg
     3001 cagaccttgc gaaagacaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     3061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     3241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     3301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     3361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     3421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     3481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     3541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     3601 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     3661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3721 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3901 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     3961 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4021 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4081 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4141 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4201 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4261 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     4321 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     4381 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4441 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4501 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4561 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4801 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4861 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4921 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5101 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5161 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5221 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5281 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5341 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5401 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5461 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5521 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5581 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5641 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5701 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5761 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5821 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5881 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5941 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6001 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6061 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6121 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6181 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6241 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6301 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6361 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6421 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6481 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6541 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6601 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6661 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6721 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6781 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6841 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6901 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6961 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7021 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7081 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7141 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     7201 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     7261 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     7321 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     7381 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     7441 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7501 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7561 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     7621 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     7681 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     7741 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     7801 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7861 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7921 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7981 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     8041 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     8101 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     8161 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     8221 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     8281 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     8341 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     8401 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     8461 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     8521 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     8581 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     8641 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     8701 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8761 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8821 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8881 gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8941 tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg
     9001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     9061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     9121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt
     9181 accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     9241 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     9301 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     9361 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt
     9421 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     9481 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     9541 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     9601 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     9661 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     9721 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     9781 caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca
     9841 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9901 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     9961 gggtttatta cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca
    10021 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
    10081 gaaaggagtg ggaatt
//
