LOCUS       sequence-338767-       13210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0385
FEATURES             Location/Qualifiers
     source          1..13210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6148..6736
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6201..6221)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6619..6630)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6808..7041
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7119..7240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7156..7175)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7210..7229
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7280..7415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7435..7454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7436..7454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7464..7481)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7464..7480)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7473..7495)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7622..8077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7709..7728)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7919..7940
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8103..8207
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8208..8276,8277..9068)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8426..8445)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9239..9827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9728..9747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9981..9998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10115..10136
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10151..10156,10157..10174,10175..10181)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10189..10205
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10194..10216
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10213..10229
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10213..10229
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10248..10268
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10250..10268
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10296..10522
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10523..10703
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10750..10875
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11368..11601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11786..11830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12061..12271
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12083..12279
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12130..12265
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12311..12913
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12311..12330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12807..12827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12956..13073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtc
     1201 cactccagac ccacccctgg gcggaactcc tcggccaggt ccttccccgg gccctggccc
     1261 ttcccctgga gccatgctgg gccctagccc gggtccctcg ccgggctccg cccacagcat
     1321 gatggggccc agcccagggc cgccctcagc aggacacccc atccccaccc aggggcctgg
     1381 agggtaccct caggacaaca tgcaccagat gcacaagccc atggagtcca tgcatgagaa
     1441 gggcatgtcg gacgacccgc gctacaacca gatgaaagga atggggatgc ggtcaggggg
     1501 ccatgctggg atggggcccc cgcccagccc catggaccag cactcccaag gttacccctc
     1561 gcccctgggt ggctctgagc atgcctctag tccagttcca gccagtggcc cgtcttcggg
     1621 gccccagatg tcttccgggc caggaggtgc cccgctggat ggtgctgacc cccaggcctt
     1681 ggggcagcag aaccggggcc caaccccatt taaccagaac cagctgcacc agctcagagc
     1741 tcagatcatg gcctacaaga tgctggccag ggggcagccc ctccccgacc acctgcagat
     1801 ggcggtgcag ggcaagcggc cgatgcccgg gatgcagcag cagatgccaa cgctacctcc
     1861 accctcggtg tccgcaacag gacccggccc tggccctggc cctggccccg gcccgggtcc
     1921 cggcccggca cctccaaatt acagcaggcc tcatggtatg ggagggccca acatgcctcc
     1981 cccaggaccc tcgggcgtgc cccccgggat gccaggccag cctcctggag ggcctcccaa
     2041 gccctggcct gaaggaccca tggcgaatgc tgctgccccc acgagcaccc ctcagaagct
     2101 gattcccccg cagccaacgg gccgcccttc ccccgcgccc cctgccgtcc cacccgccgc
     2161 ctcgcccgtg atgccaccgc agacccagtc ccccgggcag ccggcccagc ccgcgcccat
     2221 ggtgccactg caccagaagc agagccgcat cacccccatc cagaagccgc ggggcctcga
     2281 ccctgtggag atcctgcagg agcgcgagta caggctgcag gctcgcatcg cacaccgaat
     2341 tcaggaactt gaaaaccttc ccgggtccct ggccggggat ttgcgaacca aagcgaccat
     2401 tgagctcaag gccctcaggc tgctgaactt ccagaggcag ctgcgccagg aggtggtggt
     2461 gtgcatgcgg agggacacag cgctggagac agccctcaat gctaaggcct acaagcgcag
     2521 caagcgccag tccctgcgcg aggcccgcat cactgagaag ctggagaagc agcagaagat
     2581 cgagcaggag cgcaagcgcc ggcagaagca ccaggaatac ctcaatagca ttctccagca
     2641 tgccaaggat ttcaaggaat atcacagatc cgtcacaggc aaaatccaga agctgaccaa
     2701 ggcagtggcc acgtaccatg ccaacacgga gcgggagcag aagaaagaga acgagcggat
     2761 cgagaaggag cgcatgcgga ggctcatggc tgaagatgag gaggggtacc gcaagctcat
     2821 cgaccagaag aaggacaagc gcctggccta cctcttgcag cagacagacg agtacgtggc
     2881 taacctcacg gagctggtgc ggcagcacaa ggctgcccag gtcgccaagg agaaaaagaa
     2941 gaaaaagaaa aagaagaagg cagaaaatgc agaaggacag acgcctgcca ttgggccgga
     3001 tggcgagcct ctggacgaga ccagccagat gagcgacctc ccggtgaagg tgatccacgt
     3061 ggagagtggg aagatcctca caggcacaga tgcccccaaa gccgggcagc tggaggcctg
     3121 gctcgagatg aacccggggt atgaagtagc tccgaggtct gatagtgaag aaagtggctc
     3181 agaagaagag gaagaggagg aggaggaaga gcagccgcag gcagcacagc ctcccaccct
     3241 gcccgtggag gagaagaaga agattccaga tccagacagc gatgacgtct ctgaggtgga
     3301 cgcgcggcac atcattgaga atgccaagca agatgtcgat gatgaatatg gcgtgtccca
     3361 ggcccttgca cgtggcctgc agtcctacta tgccgtggcc catgctgtca ctgagagagt
     3421 ggacaagcag tcagcgctta tggtcaatgg tgtcctcaaa cagtaccaga tcaaaggttt
     3481 ggagtggctg gtgtccctgt acaacaacaa cctgaacggc atcctggccg acgagatggg
     3541 cctggggaag accatccaga ccatcgcgct catcacgtac ctcatggagc acaaacgcat
     3601 caatgggccc ttcctcatca tcgtgcctct ctcaacgctg tccaactggg cgtacgagtt
     3661 tgacaagtgg gccccctccg tggtgaaggt gtcttacaag ggatccccag cagcaagacg
     3721 ggcctttgtc ccccagctcc ggagtgggaa gttcaacgtc ttgctgacga cgtacgagta
     3781 catcatcaaa gacaagcaca tcctcgccaa gatccgttgg aagtacatga ttgtggacga
     3841 aggtcaccgc atgaagaacc accactgcaa gctgacgcag gtgctcaaca cgcactatgt
     3901 ggcaccccgc cgcctgctgc tgacgggcac accgctgcag aacaagcttc ccgagctctg
     3961 ggcgctgctc aacttcctgc tgcccaccat cttcaagagc tgcagcacct tcgagcagtg
     4021 gtttaacgca ccctttgcca tgaccgggga aaaggtggac ctgaatgagg aggaaaccat
     4081 tctcatcatc cggcgtctcc acaaagtgct gcggcccttc ttgctccgac gactcaagaa
     4141 ggaagtcgag gcccagttgc ccgaaaaggt ggagtacgtc atcaagtgcg acatgtctgc
     4201 gctgcagcga gtgctctacc gccacatgca ggccaagggc gtgctgctga ctgatggctc
     4261 cgagaaggac aagaagggca aaggcggcac caagaccctg atgaacacca tcatgcagct
     4321 gcggaagatc tgcaaccacc cctacatgtt ccagcacatc gaggagtcct tttccgagca
     4381 cttggggttc actggcggca ttgtccaagg gctggacctg taccgagcct cgggtaaatt
     4441 tgagcttctt gatagaattc ttcccaaact ccgagcaacc aaccacaaag tgctgctgtt
     4501 ctgccaaatg acctccctca tgaccatcat ggaagattac tttgcgtatc gcggctttaa
     4561 atacctcagg cttgatggaa ccacgaaggc ggaggaccgg ggcatgctgc tgaaaacctt
     4621 caacgagccc ggctctgagt acttcatctt cctgctcagc acccgggctg gggggctcgg
     4681 cctgaacctc cagtcggcag acactgtgat catttttgac agcgactgga atcctcacca
     4741 ggacctgcaa gcgcaggacc gagcccaccg catcgggcag cagaacgagg tgcgtgtgct
     4801 ccgcctctgc accgtcaaca gcgtggagga gaagatccta gctgcagcca agtacaagct
     4861 caacgtggac cagaaggtga tccaggccgg catgttcgac cagaagtcct ccagccatga
     4921 gcggcgcgcc ttcctgcagg ccatcctgga gcacgaggag caggatgagg aggaagacga
     4981 ggtgcccgac gacgagaccg tcaaccagat gatcgcccgg cacgaggagg agtttgatct
     5041 gttcatgcgc atggacctgg accgcaggcg cgaggaggcc cgcaacccca agcggaagcc
     5101 gcgcctcatg gaggaggacg agctcccctc gtggatcatc aaggacgacg cggaggtgga
     5161 gcggctgacc tgtgaggagg aggaggagaa gatgttcggc cgtggctccc gccaccgcaa
     5221 ggaggtggac tacagcgact cactgacgga gaagcagtgg ctcaaggcca tcgaggaggg
     5281 cacgctggag gagatcgaag aggaggtccg gcagaagaaa tcatcacgga agcgcaagcg
     5341 agacagcgac gccggctcct ccaccccgac caccagcacc cgcagccgcg acaaggacga
     5401 cgagagcaag aagcagaaga agcgcgggcg gccgcctgcc gagaaactct cccctaaccc
     5461 acccaacctc accaagaaga tgaagaagat tgtggatgcc gtgatcaagt acaaggacag
     5521 cagcagtgga cgtcagctca gcgaggtctt catccagctg ccctcgcgaa aggagctgcc
     5581 cgagtactac gagctcatcc gcaagcccgt ggacttcaag aagataaagg agcgcattcg
     5641 caaccacaag taccgcagcc tcaacgacct agagaaggac gtcatgctcc tgtgccagaa
     5701 cgcacagacc ttcaacctgg agggctccct gatctatgaa gactccatcg tcttgcagtc
     5761 ggtcttcacc agcgtgcggc agaaaatcga gaaggaggat gacagtgaag gcgaggagag
     5821 tgaggaggag gaagagggcg aggaggaagg ctccgaatcc gaatctcggt ccgtcaaagt
     5881 gaagatcaag cttggccgga aggagaaggc acaggaccgg ctgaagggcg gccggcggcg
     5941 gccgagccga gggtcccgag ccaagccggt cgtgagtgac gatgacagtg aggaggaaca
     6001 agaggaggac cgctcaggaa gtggcagcga agaagactag taatgaacta gtccgtaact
     6061 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaac tatgatgggc
     6121 tccaaccgca caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6181 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6241 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6301 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6361 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6421 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6481 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6541 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6601 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6661 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6721 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     6781 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     6841 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7021 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     7081 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7201 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7261 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7321 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7381 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7441 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7501 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7561 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7621 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7681 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7741 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     7801 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     7861 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     7921 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     7981 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8041 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8101 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8161 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8221 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8281 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8341 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8401 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8461 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8521 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8581 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8641 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8701 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8761 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8821 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     8881 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     8941 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9001 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9061 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9121 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9181 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9241 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9301 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9361 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9421 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9481 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9541 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9601 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9661 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9721 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     9781 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     9841 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     9901 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     9961 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10021 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10081 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10141 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10201 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10261 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
    10321 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
    10381 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
    10441 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
    10501 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
    10561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10681 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    10741 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    10801 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    10861 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    10921 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    10981 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    11041 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    11101 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    11161 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
    11221 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    11281 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    11341 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    11401 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    11461 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    11521 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    11581 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    11641 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    11701 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    11761 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    11821 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    11881 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    11941 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    12001 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    12061 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
    12121 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    12181 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    12241 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
    12301 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    12361 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    12421 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    12481 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
    12541 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    12601 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    12661 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    12721 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    12781 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    12841 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    12901 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    12961 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    13021 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    13081 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    13141 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    13201 agtgggaatt
//
