LOCUS       sequence-338769-       10261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10261)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1690
FEATURES             Location/Qualifiers
     source          1..10261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2666..2675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3199..3787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3252..3272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3670..3681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3859..4092
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4170..4291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4207..4226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4261..4280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4331..4466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4486..4505)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4487..4505)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4515..4532)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4515..4531)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4524..4546)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4673..5128
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4760..4779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4970..4991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5154..5258
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5259..5327,5328..6119)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5477..5496)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6290..6878
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6779..6798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7032..7049
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7166..7187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7202..7207,7208..7225,7226..7232)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7240..7256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7245..7267
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7264..7280
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7264..7280
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7299..7319
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7301..7319
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7347..7573
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7574..7754
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7801..7926
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8419..8652
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8837..8881
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9112..9322
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9134..9330
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9181..9316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9362..9964
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9362..9381)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9858..9878
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10007..10124
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 ttctaaactg agccagctgc agacggagct cctggcggcc ctgctcgagt cagggctgag
     1261 caaagaggca ctgatccagg cactgggtga gccggggccc tacctcctgg ctggagaagg
     1321 ccccctggac aagggggagt cctgcggcgg cggtcgaggg gagctggctg agctgcccaa
     1381 tgggctgggg gagactcggg gctccgagga cgagacggac gacgatgggg aagacttcac
     1441 gccacccatc ctcaaagagc tggagaacct cagccctgag gaggcggccc accagaaagc
     1501 cgtggtggag acccttctgc aggaggaccc gtggcgtgtg gcgaagatgg tcaagtccta
     1561 cctgcagcag cacaacatcc cacagcggga ggtggtcgat accactggcc tcaaccagtc
     1621 ccacctgtcc caacacctca acaagggcac tcccatgaag acgcagaagc gggccgccct
     1681 gtacacctgg tacgtccgca agcagcgaga ggtggcgcag cagttcaccc atgcagggca
     1741 gggagggctg attgaagagc ccacaggtga tgagctacca accaagaagg ggcggaggaa
     1801 ccgtttcaag tggggcccag catcccagca gatcctgttc caggcctatg agaggcagaa
     1861 gaaccctagc aaggaggagc gagagacgct agtggaggag tgcaataggg cggaatgcat
     1921 ccagagaggg gtgtccccat cacaggcaca ggggctgggc tccaacctcg tcacggaggt
     1981 gcgtgtctac aactggtttg ccaaccggcg caaagaagaa gccttccggc acaagctggc
     2041 catggacacg tacagcgggc cccccccagg gccaggcccg ggacctgcgc tgcccgctca
     2101 cagctcccct ggcctgcctc cacctgccct ctcccccagt aaggtccacg gtgtgcgcta
     2161 tggacagcct gcgaccagtg agactgcaga agtaccctca agcagcggcg gtcccttagt
     2221 gacagtgtct acacccctcc accaagtgtc ccccacgggc ctggagccca gccacagcct
     2281 gctgagtaca gaagccaagc tggtctcagc agctgggggc cccctccccc ctgtcagcac
     2341 cctgacagca ctgcacagct tggagcagac atccccaggc ctcaaccagc agccccagaa
     2401 cctcatcatg gcctcacttc ctggggtcat gaccatcggg cctggtgagc ctgcctccct
     2461 gggtcctacg ttcaccaaca caggtgcctc caccctggtc atcggcctgg cctccacgca
     2521 ggcacagagt gtgccggtca tcaacagcat gggcagcagc ctgaccaccc tgcagcccgt
     2581 ccagttctcc cagccgctgc acccctccta ccagcagccg ctcatgccac ctgtgcagag
     2641 ccatgtgacc cagagcccct tcatggccac catggctcag ctgcagagcc cccacgccct
     2701 ctacagccac aagcccgagg tggcccagta cacccacacg ggcctgctcc cgcagactat
     2761 gctcatcacc gacaccacca acctgagcgc cctggccagc ctcacgccca ccaagcaggt
     2821 cttcacctca gacactgagg cctccagtga gtccgggctt cacacgccgg catctcaggc
     2881 caccaccctc cacgtcccca gccaggaccc tgccagcatc cagcacctgc agccggccca
     2941 ccggctcagc gccagcccca cagtgtcctc cagcagcctg gtgctgtacc agagctcaga
     3001 ctccagcaat ggccagagcc acctgctgcc atccaaccac agcgtcatcg agaccttcat
     3061 ctccacccag atggcctctt cctcccagta gtaatgaact agtccgtaac ttgaaagtat
     3121 ttcgatttct tggctttata tatcttgtgg aaaggacgac ggccagcttt cgcagagtgt
     3181 gttacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     3241 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     3301 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     3361 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     3421 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     3481 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     3541 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3601 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     3661 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3721 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3781 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3841 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3901 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     4021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     4081 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     4141 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     4201 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     4321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4441 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     4501 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4681 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4741 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4801 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4861 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4921 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4981 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5041 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5101 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5161 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5221 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5281 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5341 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5401 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5461 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5521 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5581 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5641 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5701 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5761 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5821 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5881 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5941 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6001 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6061 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6121 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6181 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6241 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6301 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6361 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6421 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6481 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6541 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6601 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6661 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6721 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6781 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6841 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6901 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6961 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7021 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     7141 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     7201 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     7261 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     7321 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     7381 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7441 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7501 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7561 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7621 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7681 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7741 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7801 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7861 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7921 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7981 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     8041 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     8101 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     8161 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     8221 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     8281 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     8341 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8401 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8461 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8521 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8581 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8641 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8701 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8761 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8821 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8881 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8941 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     9001 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     9061 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     9121 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     9181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     9241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9301 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     9361 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     9421 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     9481 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     9541 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     9601 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     9661 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9721 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9781 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9841 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9901 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9961 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
    10021 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
    10081 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
    10141 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
    10201 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
    10261 t
//
