LOCUS       sequence-338772-       10549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing the UBTF transcription factor 
            ORF with a unique 24-bp barcode. Barcodes facilitate identification 
            of transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0139
FEATURES             Location/Qualifiers
     source          1..10549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3134..3145
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     misc_feature    3487..4075
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3540..3560)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3958..3969)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4147..4380
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4458..4579
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4495..4514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4549..4568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4619..4754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4774..4793)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4775..4793)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4803..4820)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4803..4819)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4812..4834)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4961..5416
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5048..5067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5258..5279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5442..5546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5547..5615,5616..6407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5765..5784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6578..7166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7067..7086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7320..7337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7454..7475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7490..7495,7496..7513,7514..7520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7528..7544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7533..7555
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7552..7568
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7552..7568
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7587..7607
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7589..7607
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7635..7861
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7862..8042
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8089..8214
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8707..8940
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9125..9169
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9400..9610
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9422..9618
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9469..9604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9650..10252
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9650..9669)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10146..10166
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10295..10412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 cggagaagcc gactgcccca cagacctgga aatggccgcc cccaaaggcc aagaccgttg
     1261 gtcccaggaa gacatgctga ctttgctgga atgcatgaag aacaaccttc catccaatga
     1321 cagctccaag ttcaaaacca ccgaatcaca catggactgg gaaaaagtag catttaaaga
     1381 cttttctgga gacatgtgca agctcaaatg ggtggagatt tctaatgagg tgaggaagtt
     1441 ccgtacattg acagaattga tcctcgatgc tcaggaacat gttaaaaatc cttacaaagg
     1501 caaaaaactc aagaaacacc cagacttccc aaagaagccc ctgacccctt atttccgctt
     1561 cttcatggag aagcgggcca agtatgcgaa actccaccct gagatgagca acctggacct
     1621 aaccaagatt ctgtccaaga aatacaagga gcttccggag aagaagaaga tgaaatatat
     1681 tcaggacttc cagagagaga aacaggagtt cgagcgaaac ctggcccgat tcagggagga
     1741 tcaccccgac ctaatccaga atgccaagaa atcggacatc ccagagaagc ccaaaacccc
     1801 ccagcagctg tggtacaccc acgagaagaa ggtgtatctc aaagtgcggc cagatgagat
     1861 catgagagac tatatccaga agcacccaga gctgaacatc agtgaggagg gtatcaccaa
     1921 gtccaccctc accaaggccg aacgccagct caaggacaag tttgacgggc gacccaccaa
     1981 gccacctccg aacagctact cgctgtactg cgcagagctc atggccaaca tgaaggacgt
     2041 gcccagcaca gagcgcatgg tgctgtgcag ccagcagtgg aagctgctgt cccagaagga
     2101 gaaggacgcc tatcacaaga agtgtgatca gaaaaagaaa gattacgagg tggagctgct
     2161 ccgtttcctc gagagcctgc ctgaggagga gcagcagcgg gtcttggggg aagagaagat
     2221 gctgaacatc aacaagaagc aggccaccag ccccgcctcc aagaagccag cccaggaagg
     2281 gggcaagggc ggctccgaga agcccaagcg gcccgtgtcg gccatgttca tcttctcgga
     2341 ggagaaacgg cggcagctgc aggaggagcg gcctgagctc tccgagagcg agctgacccg
     2401 cctgctggcc cgaatgtgga acgacctgtc tgagaagaag aaggccaagt acaaggcccg
     2461 agaggcggcg ctcaaggctc agtcggagag gaagcccggc ggggagcgcg aggaacgggg
     2521 caagctgccc gagtccccca aaagagctga ggagatctgg caacagagcg ttatcggcga
     2581 ctacctggcc cgcttcaaga atgaccgggt gaaggccttg aaagccatgg aaatgacctg
     2641 gaataacatg gaaaagaagg agaaactgat gtggattaag aaggcagccg aagaccaaaa
     2701 gcgatatgag agagagctga gtgagatgcg ggcacctcca gctgctacaa attcttccaa
     2761 gaagatgaaa ttccagggag aacccaagaa gcctcccatg aacggttacc agaagttctc
     2821 ccaggagctg ctgtccaatg gggagctgaa ccacctgccg ctgaaggagc gcatggtgga
     2881 gatcggcagt cgctggcagc gcatctccca gagccagaag gagcactaca aaaagctggc
     2941 cgaggagcag caaaagcagt acaaggtgca cctggacctc tgggttaaga gcctgtctcc
     3001 ccaggaccgt gcagcatata aagagtacat ctccaataaa cgtaagagca tgaccaagct
     3061 gcgaggccca aaccccaaat ccagccggac tactctgcag tccaagtcgg agtccgagga
     3121 ggatgatgaa gaggatgagg atgacgagga cgaggatgaa gaagaggaag atgatgagaa
     3181 tggggactcc tctgaagatg gcggcgactc ctctgagtcc agcagcgagg acgagagcga
     3241 ggatggggat gagaatgaag aggatgacga ggacgaagac gacgacgagg atgacgatga
     3301 ggatgaagat aatgagtccg agggcagcag ctccagctcc tcctcctcag gggactcctc
     3361 agactctgac tccaactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     3421 gctttatata tcttgtggaa aggacgacgc cgcacaagtg tcacagcggt gacgcgttaa
     3481 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3541 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3601 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3661 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3721 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3781 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     3841 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     3901 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     3961 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4021 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     4081 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4141 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4201 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4261 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4321 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4381 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     4441 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4501 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4561 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4621 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4681 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4741 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4801 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4861 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4921 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4981 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     5041 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     5101 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     5161 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     5221 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     5281 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     5341 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     5401 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5461 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7561 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7621 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7681 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7741 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7801 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7861 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7921 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7981 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8041 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8101 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8161 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8221 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8281 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8341 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8401 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8461 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8521 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8581 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8641 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8701 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8761 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8821 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8881 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8941 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9001 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9061 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9121 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9181 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9241 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9301 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     9361 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9421 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9481 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9541 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9601 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     9661 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     9721 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     9781 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     9841 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     9901 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     9961 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
    10021 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
    10081 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
    10141 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
    10201 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
    10261 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10321 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10381 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10441 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
    10501 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
