LOCUS       sequence-338820-        8825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low level expression of mKeima-PIM. Can be used for ratiometric 
            detection of inducible aggregate acidification after addition of 
            AP20187. mKeima fluorophore allows for simultaneous GFP-tagged 
            imaging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
  TITLE     Direct observation of aggregate-triggered selective autophagy in 
            human cells.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 
            10.1242/jcs.258824. Epub 2021 Oct 6.
  PUBMED    34447998
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19). pii: 271972. doi"; 
            pages: " 10.1242/jcs.258824. Epub 2021 Oct 6"
COMMENT     Sequence Label: pBA-mKeima-PIM
FEATURES             Location/Qualifiers
     source          1..8825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     928..950
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1029..1349
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1356..1676
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             join(1692..1694,1695..2354)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG"
     CDS             3048..3368
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3375..3695
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3375..3695
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    3981..4115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4018..4037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4072..4091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4130..4146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4130..4146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4143..4165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4154..4170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4178..4184,4185..4202,4203..4208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4223..4244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4361..4378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4532..5120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4612..4631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5291..6082,6083..6151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5914..5933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6152..6256)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6324..6342
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6380..6402)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6502..6521
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6715..6737
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6729..6746
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6730..6746
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8545..8820
                     /label=chicken beta-actin promoter
ORIGIN
        1 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
       61 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      121 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
      181 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
      241 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
      301 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
      361 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
      421 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
      481 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
      541 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      601 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
      661 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      721 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      781 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
      841 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt
      961 atatcttccc ttctctgttc ctccgcagcc cccaagctag cgctaagctt gccaccatgc
     1021 gctctagagg cgtccaagtc gaaaccatta gtcccggcga tggcagaaca tttcctaaaa
     1081 ggggacaaac atgtgtcgtc cattatacag gcatgttgga ggacggcaaa aaggtggaca
     1141 gtagtagaga tcgcaataaa cctttcaaat tcatgttggg aaaacaagaa gtcattaggg
     1201 gatgggagga gggcgtggct caaatgtccg tcggccaacg cgctaagctc accatcagcc
     1261 ccgactacgc atacggcgct accggacatc ccggaattat tccccctcac gctaccttgg
     1321 tgtttgacgt cgaactgttg aagctcgaga ctagaggagt gcaggtggag actatctccc
     1381 caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggagcac tacaccggga
     1441 tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta
     1501 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
     1561 gtcagagagc caaactgact atatctccag attgtgccta tggtgccact gggcacccag
     1621 gcatcatccc accacatacc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta
     1681 gtgaaccggt agtgagtgtg atcgctaaac aaatgaccta caaggtttat atgtcaggca
     1741 cggtcaatgg acactacttt gaggtcgaag gcgatggaaa aggaaagcct tacgagggag
     1801 agcagacagt aaagctcact gtcaccaagg gtggacctct gccatttgct tgggatattt
     1861 tatcaccaca gcttcagtac ggaagcatac cattcaccaa gtaccctgaa gacatccctg
     1921 attatttcaa gcagtcattc cctgagggat atacatggga gaggagcatg aactttgaag
     1981 atggtgcagt gtgtactgtc agcaatgatt ccagcatcca aggcaactgt ttcatctaca
     2041 atgtcaaaat ctctggtgag aactttcctc ccaatggacc tgttatgcag aagaagacac
     2101 agggctggga acccagcact gagcgtctct ttgcacgaga tggaatgctg ataggaaacg
     2161 attatatggc tctgaagttg gaaggaggtg gtcactattt gtgtgaattt aaatctactt
     2221 acaaggcaaa gaagcctgtg aggatgccag ggcgccacga gattgaccgc aaactggatg
     2281 taaccagtca caacagggat tacacatctg ttgagcagtg tgaaatagcc attgcacgcc
     2341 actctttgct cggtggcgcg cctcgctcac gcggcgttca ggtggagact ataagcccgg
     2401 gggatggtcg cacttttcct aaacgaggcc agacgtgcgt agttcactat accggcatgc
     2461 ttgaagacgg caagaaggtg gatagctcta gggaccggaa taagcccttc aagttcatgc
     2521 ttggtaagca agaagtgatc cggggttggg aagagggggt tgcccaaatg tcagtcggac
     2581 aacgggcgaa gttgactatt tctcctgatt acgcttatgg tgcgacggga catcctggca
     2641 tcataccgcc tcacgccact cttgtctttg atgtggaact tttgaagctg gaaacgcgag
     2701 gggttcaagt tgagaccatt tcaccgggcg atggcagaac attcccgaaa aggggacaaa
     2761 cgtgtgtagt acactatacg ggcatgctcg aagacgggaa aaaggttgac tcaagtagag
     2821 atcggaacaa gcctttcaag ttcatgttgg ggaaacaaga ggtaatacgc gggtgggagg
     2881 agggagtcgc tcagatgtct gtaggccaaa gggcgaagct caccatatca ccagattacg
     2941 cttacggggc gacgggccat cccggaataa tacctccgca cgcgacactt gtttttgatg
     3001 ttgaattgct taagttggag accagcagat ctgtcgaccg ctctagagga gtgcaggtgg
     3061 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
     3121 actacaccgg gatgcttgaa gatggaaaga aatttgactc ctcccgggac agaaacaagc
     3181 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
     3241 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
     3301 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
     3361 aactggaaac tagaggcgtg caggtcgaga ccatcagccc cggcgacggc cgcacctttc
     3421 ccaagagagg ccagacttgc gtggtccact acaccggcat gctggaggac ggcaagaagt
     3481 tcgacagcag ccgcgaccgc aacaagccct tcaagttcat gctgggcaaa caggaagtga
     3541 tccgcggctg ggaggaaggc gtggctcaga tgagcgtggg gcagcgggcc aagctgacca
     3601 tcagccccga ctatgcctac ggcgccaccg gccaccccgg catcatcccc ccccacgcca
     3661 ccctggtgtt cgacgtggag ctgctgaagc tggagactag ttaggaattc aggatccagt
     3721 cgactagtac ttctcgagct ctgtacatgt ccgcggtcgc gacgtacgcg tatcgatggc
     3781 gccagctgca ggcggccgcc atatgcatcc taggcctatt aatattccgg agtatacgta
     3841 gccggctaac aaccggtacc tctagataag taatgatcat aatcagccat atcacatctg
     3901 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3961 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     4021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4081 ccaaactcat caatgtatct tatcatgtct ggatcaattc gtaatcatgg tcatagctgt
     4141 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4201 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4261 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4321 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4381 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4441 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4501 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4561 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4621 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4681 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4741 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4801 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4861 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4921 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacagtaga aggacagtat
     4981 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5041 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5101 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5161 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5221 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5281 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5341 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5401 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5461 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5521 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5581 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5641 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5701 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5761 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5821 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5881 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5941 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6001 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6061 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6121 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6181 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6241 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     6301 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6481 gtcggggttg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     6541 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     6601 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     6661 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     6721 cacgacgttg taaaacgacg gccagtgcca agttgggatc tttgcattgg cccacggctc
     6781 tcaggatggg gatgctcccc ttcagcaccc ggttcccctt ggaaactgat ggtcctggct
     6841 ctgtggcatg gcagtggcac tgtgaggagc ccctaccagc agcacacagt gggtttggca
     6901 ctgccacgct ccggatgccg cgctctgatc caaccccata atcaagggaa cccgaattgc
     6961 cccatcattg cccccaccac ccccatcctg ccgggccctc acaccccacg ctgccttgtg
     7021 gtgacattcc ccagcccaaa cccacggctt catggctacc gcggggcatt tcccattgcc
     7081 gccccattat cagctctgca cacctcccgc tgtacccatg cctcgtggct gcccttcttt
     7141 gacgtataat cttctaatta atacccggcc ttgtcaaagt ggagcacaaa cgttaattaa
     7201 ttccccagca ggcaggtaat taacagtgtg actccctttt tgctgcgagt ggggctgata
     7261 cagagagatg tggcactatg gagcccacgg ggtcctggca ctgggtgccc acggaggtcc
     7321 ccatgtgctg cagtgtcacc gcctccgagg tgacagtatt gtccctgcgg tgtccctgca
     7381 gctcagctct gtccacaggg ccacctccag tttggagggg acacaatgca gccccgatgc
     7441 aacccatcct cgcagcatcc cagggacaaa gaccccactg caagaccgca cacagggctg
     7501 ggtcccgctc ccctaatatc tacagtgctt ttgcatggcc ccttaatcaa tgcagttaat
     7561 cagcatgcgc tcatgcaccg ctctggagct gcaaagcccc tcgcagcgct gctcaccaac
     7621 accgcgcacc gccccggccc agcctgcagc acgcgctgca aacaggaaag aaacaaaata
     7681 ttgcccaaat gtaggcaaag gcattcggct gccttgacct ccgccgggcc gggccctgcc
     7741 tgactcagct ccttactcag cgctcgcttc ctccctccgg ctgccaccgc cgcagcgcac
     7801 accctgacaa agagtggccc ttaacgggct ctgaggtgca cccagcagtg cactcagcag
     7861 tccaagggcc ggcctggagg tttgcaccgc tacgtgctga cattagcatt gaacttggcc
     7921 ctgggtagtg ctgcaggccg ggcggggtgg gtgtagagag tgcagcgcgc gttgcacccg
     7981 gtgccccttc ccctcccttg catcccagca ggctgcaccc cagcaccagg cccgtgcatg
     8041 catgctcctg gtgttattgc agcctggtgc atgcatgcgt cttagtggtg cagcgctgtg
     8101 catgcatcct ccttggtgtg tagcagctta gtgcatgcat acccctcggt gttattgctg
     8161 ctctgtgcag gcacgctcat tgtatcactt catcccagtg catgcactca cactggagcg
     8221 attgctgctc ggtgcacgca cactcattgt atcacgtcag ctcagtggct gcacgcacac
     8281 cggtgttatt gctgctcggt gcgtgcatgc acatcagtgt cgctgcagct cagtgcatgc
     8341 acgctcattg cccatcgcta tccctgcctc tcctgctggc gctccccggg aggtgacttc
     8401 aaggggaccg caggaccacc tcgggggtgg ggggagggct gcacacgcgg accccgctcc
     8461 ccctccccaa caaagcactg tggaatcaaa aaggggggag gggggatgga ggggcgcgtc
     8521 acacccccgc cccacaccct cacctcgagg tgagccccac gttctgcttc actctcccca
     8581 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     8641 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     8701 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     8761 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     8821 ggagt
//
