LOCUS       sequence-33884-e         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA
  TITLE     Differential regulation of JAMM domain deubiquitinating enzyme 
            activity within the RAP80 complex.
  JOURNAL   J Biol Chem. 2010 Oct 1. 285(40):30971-81.
  PUBMED    20656689
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Oct 1. 285(40):30971-81."
COMMENT     Sequence Label: pOZ-N-FH Abraxas
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcctctaga ctgccagatc ttccgctcga tgaaccatgg actacaagga cgacgatgac
       61 aagctcgatg gaggataccc ctacgacgtg cccgactacg ccggaggact cgaggagggg
      121 gagagtacgt cggcggtgct ctcgggcttt gtgctcggcg cactcgcttt ccagcacctc
      181 aacacggact cggacacgga aggttttctt cttggggaag taaaaggtga agccaagaac
      241 agcattactg attcccaaat ggatgatgtt gaagttgttt atacaattga cattcagaaa
      301 tatattccat gctatcagct ttttagcttt tataattctt caggcgaagt aaatgagcaa
      361 gcactgaaga aaatattatc aaatgtcaaa aagaatgtgg taggttggta caaattccgt
      421 cgtcattcag atcagatcat gacgtttagg gaaagactcc ttcacaaaaa cttgcaggag
      481 catttttcaa accaagacct tgtttttctg ctattaacac caagtataat aacagaaagc
      541 tgctctactc atcgactgga acattcctta tataaacctc aaaaaggact ttttcacagg
      601 gtacctttag tggttgccaa tctgggcatg tctgaacaac tgggttataa aactgtatca
      661 ggttcctgta tgtccactgg ttttagccga gcagtacaaa cacacagctc taaatttttt
      721 gaagaagatg gatccttaaa gga
//
