LOCUS       sequence-33885-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA
  TITLE     Differential regulation of JAMM domain deubiquitinating enzyme 
            activity within the RAP80 complex.
  JOURNAL   J Biol Chem. 2010 Oct 1. 285(40):30971-81.
  PUBMED    20656689
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Oct 1. 285(40):30971-81."
COMMENT     Sequence Label: pOZ-N-FH BRCC36
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             82..108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             82..108
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tggaccatcc tctagactgc cagatcttcc gctcgatgaa ccatggacta caaggacgac
       61 gatgacaagc tcgatggagg atacccctac gacgtgcccg actacgccgg aggactcgag
      121 aattcgcggc cgcccgcggt acaggtggtg caggcggtgc aggcggttca tctcgagtct
      181 gacgctttcc tcgtttgtct caaccacgct ctgagcacag agaaggagga agtaatgggg
      241 ctgtgcatag gggagttgaa cgatgataca aggagtgact ccaaatttgc atatactgga
      301 actgaaatgc gcacagttgc tgaaaaggtt gatgccgtca gaattgttca cattcattct
      361 gtcatcatct tacgacgttc tgataagcga aaggacaggg tagagatttc tccagagcag
      421 ctgtctgcag cttcaacaga ggcagagagg ttggctgaac tgacaggccg ccccatgaga
      481 gttgtgggct ggtatcattc ccatcctcat ataactgttt ggccttcaca tgttgatgtt
      541 cgcacacaag ccatgtacca gatgatggat caaggctttg taggacttat tttttcctgt
      601 ttcatagaag ataagaacac aaagactggc cgggtactct acacttgctt ccaatccata
      661 caggcccaaa agagttcaga gtcccttcat ggtccacgag acttctggag ctccagccag
      721 cacatctcca ttgagggcca gaaggaaga
//
