LOCUS       sequence-338853-       10004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reporter plasmid for pylBCD variant in pBAD backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Ho JML, Miller CA, Smith KA, Mattia JR, Bennett MR
  TITLE     Improved pyrrolysine biosynthesis through phage assisted 
            non-continuous directed evolution of the complete pathway.
  JOURNAL   Nat Commun. 2021 Jun 24;12(1):3914. doi: 10.1038/s41467-021-24183-9.
  PUBMED    34168131
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-24"; volume: "12(1):3914. doi"; pages: " 
            10.1038/s41467-021-24183-9"
COMMENT     Sequence Label: pJH645
FEATURES             Location/Qualifiers
     source          1..10004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1144..2067
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2116..3198
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3213..4199
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="M*KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYEN
                     HFSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFIL
                     GFSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHA
                     YTPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSE
                     IDHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECI
                     TAAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4378..5490
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     5594..5614
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        complement(join(5787..5792,5793..5809,5810..5815))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      5927..6472
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     6373..6392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6589..6606
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             6895..7554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6961..6980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9559..9606
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(9693..10004,1..1131)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 tctatgattt tatgttctaa aggcggcctt tatgatgttg tagaaaatga acttggttct
       61 cgccatatca tggatgaatg gctacctcag gatgaaagtt atgttcgggc ttttccgaaa
      121 ggtaaatctg tacatctgtt ggcaggtaat gttccattat ctgggatcat gtctatatta
      181 cgcgcaattt taactaagaa tcagtgtatt ataaaaacat cgtcaaccga tccttttacc
      241 gctaatgcat tagcgttaag ttttattgat gtagacccta atcatccgat aacgcgctct
      301 ttatctgtta tatattggcc ccaccaaggt gatacatcac tcgcaaaaga aattatgcaa
      361 catgcggatg ttattgtcgc ttggggaggg ccagatgcga ttaattgggc ggtagagcat
      421 gcgccatctt atgctgatgt gattaaattt ggttctaaaa agagtctttg cattatcgat
      481 aatcctgttg atttgacgtc cgcagcgaca ggtgcggctc atgatgtttg tttttacgat
      541 cagcgagctt gtttttctgc ccaaaacata tattacatgg gaaatcatta tgaggaattt
      601 aagttagcgt tgatagaaaa acttaatcta tatgcgcata tattaccgaa tgccaaaaaa
      661 gattttgatg aaaaggcggc ctattcttta gttcaaaaag aaagcttgtt tgctggatta
      721 aaagtagagg tggatattca tcaacgttgg atgattattg agtcaaatgc aggtgtggaa
      781 tttaatcaac cacttggcag atgtgtgtac cttcatcacg tcgataatat tgagcaaata
      841 ttgccttatg ttcaaaaaaa taagacgcaa accatatcta tttttccttg ggagtcatca
      901 tttaaatatc gagatgcgtt agcattaaaa ggtgcggaaa ggattgtaga agcaggaatg
      961 aataacatat ttcgagttgg tggatctcat gacggaatga gaccgttgca acgattagtg
     1021 acatatattt ctcatgaaag gccatctaac tatacggcta aggatgttgc ggttgaaata
     1081 gaacagactc gattcctgga agaagataag ttccttgtat ttgtcccata ataggtaaaa
     1141 agtatggaaa atgaatcaaa atataaaacc atcgaccacg ttatttgtgt tgaaggaaat
     1201 aaaaaaattc atgtttggga aacgctgcca gaagaaaaca gcccaaagag aaagaatgcc
     1261 attattattg cgtctggttt tgcccgcagg atggatcatt ttgctggtct ggcggaatat
     1321 ttatcgcgga atggatttca tgtgatccgc tatgattcgc ttcaccacgt tggattgagt
     1381 tcagggacaa ttgatgaatt tacaatgtct ataggaaagc agagcttgtt agcagtggtt
     1441 gattggttaa ctacacgaaa aataaataac ttcggtatgt tggcttcaag cttatctgcg
     1501 cggatagctt atgcaagcct atctgaaatc aatgcttcgt ttttaatcac cgcagtcggt
     1561 gttgttaact taagatattc tcttgaaaga gctttagggt ttgattatct cagtctaccc
     1621 attaatgaat tgccgaataa tctagatttt gaaggccata aattgggtgc tgaagtcttt
     1681 gcgagagatt gtcttgattt tggttgggaa gatttagctt ctacaattaa taacatgatg
     1741 tatcttgata taccgtttat tgcttttact gcaaataacg ataattgggt caagcaagat
     1801 gaagttatca cattgttatc aaatattcgt agtaatcgat gcaagatata ttctttgtta
     1861 ggaagttcgc atgacttgag tgaaaattta gtggtcctgc gcaattttta tcaatcggtt
     1921 acgaaagccg ctatcgcgat ggataatgat catctggata ttgatgttga tattactgaa
     1981 ccgtcatttg aacatttaac tattgcgaca gtcaatgaac gccgaatgag aattgagatt
     2041 gaaaatcaag caatttctct gtcttaaaat ctattgagat attctatcac tcaaatagca
     2101 atataaggac tctctatgaa atttggaaac tttttgctta cataccaacc tccccaattt
     2161 tctcaaacag aggtaatgaa acgtttggtt aaattaggtc gcatctctga ggagtgtggt
     2221 tttgataccg tatggttact ggagcatcat ttcacggagt ttggtttgct tggtaaccct
     2281 tatgtcgctg ctgcatattt acttggcgcg actaaaaaat tgaatgtagg aactgccgct
     2341 attgttcttc ccacagccca tccagtacgc caacttgaag atgtgaattt attggatcaa
     2401 atgtcaaaag gacgatttcg gtttggtatt tgccgagggc tttacaacaa ggactttcgc
     2461 gtattcggca cagatatgaa taacagtcgc gccttagcgg aatgctggta cgggctgata
     2521 aagaatggca tgacagaggg atatatggaa gctgataatg aacatatcaa gttccataag
     2581 gtaaaagtaa accccgcggc gtatagcaga ggtggcgcac cggtttatgt ggtggctgaa
     2641 tcagcttcga cgactgagtg ggctgctcaa tttggcctac cgatgatatt aagttggatt
     2701 ataaatacta acgaaaagaa agcacaactt gagctttata atgaagtggc tcaagaatat
     2761 gggcacgata ttcataatat cgaccattgc ttatcatata taacatctgt agatcatgac
     2821 tcaattaaag cgaaagagat ttgccggaaa tttctggggc attggtatga ttcttatgtg
     2881 aatgctacga ctatttttga tgattcagac caaacaagag gttatgattt caataaaggg
     2941 cagtggcgtg actttgtatt aaaaggacat aaagatacta atcgccgtat tgattacagt
     3001 tacgaaatca atcccgtggg aacgccgcag gaatgtattg acataattca aaaagacatt
     3061 gatgctacag gaatatcaaa tatttgttgt ggatttgaag ctaatggaac agtagacgaa
     3121 attattgctt ccatgaagct cttccagtct gatgtcatgc catttcttaa agaaaaacaa
     3181 cgttcgctat tatattaact aaggagaaag aaatgtagaa atttggattg ttcttcctta
     3241 acttcatcaa ttcaacaact gttcaagaac aaagtatagt tcgcatgcag gaaataacgg
     3301 agtatgttga taagttgaat tttgaacaga ttttagtgta tgaaaatcat ttttcagata
     3361 atggtgttgt cggcgctcct ctgactgttt ctggttttct gctcggttta acagagaaaa
     3421 ttaaaattgg ttcattaaat cacatcatta caactcatca tcctgtccgc atagcggagg
     3481 aagcttgctt attggatcag ttaagtgaag ggagatttat tttagggttt agtgattgcg
     3541 aaaaaaaaga tgaaatgcat ttttttaatc gcccggttga atatcaacag caactatttg
     3601 aagagtgtta tgaaatcatt aacgatgctt taacaacagg ctattgtaat ccagataacg
     3661 atttttatag cttccctaaa atatctgtaa atccccatgc ttatacgcca ggcggacctc
     3721 ggaaatatgt aacagcaacc agtcatcata ttgttgagtg ggcggccaaa aaaggtattc
     3781 ctctcatctt taagtgggat gattctaatg atgttagata tgaatatgct gaaagatata
     3841 aagccgttgc ggataaatat gacgttgacc tatcagagat agaccatcag ttaatgatat
     3901 tagttaacta taacgaagat agtaataaag ctaaacaaga gacgcgtgca tttattagtg
     3961 attatgttct tgaaatgcac cctaatgaaa atttcgaaaa taaacttgaa gaaataattg
     4021 cagaaaacgc tgtcggaaat tatacggagt gtataactgc ggctaagttg gcaattgaaa
     4081 agtgtggtgc gaaaagtgta ttgctgtcct ttgaaccaat gaatgatttg atgagccaaa
     4141 aaaatgtaat caatattgtt gatgataata ttaagaagta ccacatggaa tatacctaat
     4201 agatttcgag ttgcagcgag gcggcaagtg aacgaatccc caggagcata gataactatg
     4261 tgactggggt gagtgaaagc agccaacaaa gcagcagctt gaaagatgaa gggtataaaa
     4321 gagtatgaca gcagtgctgc catactttct aatattatct tgaggagtaa aacaggtatg
     4381 acttcatatg ttgataaaca agaaattaca gcaagctcag aaattgatga tttgattttt
     4441 tcgagcgatc cattagtgtg gtcttacgac gagcaggaaa aaatcagaaa gaaacttgtg
     4501 cttgatgcat ttcgtaatca ttataaacat tgtcgagaat atcgtcacta ctgtcaggca
     4561 cacaaagtag atgacaatat tacggaaatt gatgacatac ctgtattccc aacatcggtt
     4621 tttaagttta ctcgcttatt aacttctcag gaaaacgaga ttgaaagttg gtttaccagt
     4681 agcggcacga atggtttaaa aagtcaggtg gcgcgtgaca gattaagtat tgagagactc
     4741 ttaggctctg tgagttatgg catgaaatat gttggtagtt ggtttgatca tcaaatagaa
     4801 ttagtcaatt tgggaccaga tagatttaat gctcataata tttggtttaa atatgttatg
     4861 agtttggtgg aattgttata tcctacgaca tttaccgtaa cagaagaacg aatagatttt
     4921 gttaaaacat tgaatagtct tgaacgaata aaaaatcaag ggaaagatct ttgtcttatt
     4981 ggttcgccat actttattta tttactctgc cattatatga aagataaaaa aatctcattt
     5041 tctggagata aaagccttta tatcataacc ggaggcggct ggaaaagtta cgaaaaagaa
     5101 tctctgaaac gtgatgattt caatcatctt ttatttgata ctttcaatct cagtgatatt
     5161 agtcagatcc gagatatatt taatcaagtt gaactcaaca cttgtttctt tgaggatgaa
     5221 atgcagcgta aacatgttcc gccgtgggta tatgcgcgag cgcttgatcc tgaaacgttg
     5281 aaacctgtac ctgatggaac gccggggttg atgagttata tggatgcgtc agcaaccagt
     5341 tatccagcat ttattgttac cgatgatgtc gggataatta gcagagaata tggtaagtat
     5401 cccggcgtgc tcgttgaaat tttacgtcgc gtcaatacga ggacgcagaa agggtgtgct
     5461 ttaagcttaa ccgaagcgtt tgatagttga tgacctgcag gcatgcaagc ttgcggccgc
     5521 gggccccatg gatcgatagc tggtcgacac aatctgccct ttcgaaagat cccaacgaaa
     5581 agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg
     5641 atgagctcta caaataatga gctagcccgc ctaatgagcg ggcttttttt tctcggccta
     5701 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
     5761 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
     5821 agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa gagataaatt
     5881 gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga gatcgttttg
     5941 gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga
     6001 aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagccac
     6061 caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta
     6121 aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca
     6181 agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag
     6241 tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg
     6301 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     6361 ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     6421 atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     6481 tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag gaaatctccg
     6541 ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg
     6601 agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg tgcagccttt
     6661 tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca tagtaagcca
     6721 gtatacactc cgctagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     6781 agtagctgaa caggagggac agtaagaggt tccaactttc accataatga aataagatca
     6841 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     6901 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     6961 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggacattacg
     7021 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     7081 cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg caatgaaaga cggtgagctg
     7141 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     7201 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     7261 gatgtggcgt gttacggcga aaacctggcc tatttcccta aagggtttat tgagaatatg
     7321 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     7381 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     7441 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     7501 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt
     7561 taaggcagtt attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag
     7621 cggatgaatg gcagaaattc gaaagcaaat tcgacccggg cggccgcaac ccagcgttcg
     7681 atgcttcttt gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa tattctcaac
     7741 ataaaaaact ttgtgtaata cttgtaacgc tacatggaga ttaactcaat ctagagggta
     7801 actagtatgg ataagaagcc gctggatgtt ctgatctctg cgaccggtct gtggatgtcc
     7861 cgtaccggca cgctgcacaa gatcaagcac tatgaggttt ctcgttctaa aatctacatc
     7921 gaaatggcgt gtggtgacca tctggttgtg aacaactctc gttcttgtcg taccgcacgt
     7981 gcattccgtc atcataaata ccgtaaaacc tgcaaacgtt gtcgtgtttc tgacgaagat
     8041 atcaacaact tcctgacccg ttctaccgaa ggcaaaacct ctgttaaagt taaagttgtt
     8101 tctgcgccga aagtgaaaaa agcgatgccg aaatctgttt ctcgtgcgcc gaaaccgctg
     8161 gaaaatccgg tttctgcgaa agcgtctacc gacacctctc gttctgttcc gtctccggcg
     8221 aaatctaccc cgaactctcc ggttccgacc tctgcaagtg cccccgcact tacgaagagc
     8281 cagactgaca ggcttgaagt cctgttaaac ccaaaagatg agatttccct gaattccggc
     8341 aagcctttca gggagcttga gtccgaattg ctctctcgca gaaaaaaaga cctgcagcag
     8401 atctacgcgg aagaaaggga gaattatctg gggaaactcg agcgtgaaat taccaggttc
     8461 tttgtggaca ggggttttct ggaaataaaa tccccgatcc tgatccctct tgagtatatc
     8521 gaaaggatgg gcattgataa tgataccgaa ctttcaaaac agatcttcag ggttgacaag
     8581 aacttctgcc tgagacccat gcttgctcca aacctttaca actacctgcg caagcttgac
     8641 agggccctgc ctgatccaat aaaaattttt gaaataggcc catgctacag aaaagagtcc
     8701 gacggcaaag aacacctcga agagtttacc atgctgaact tctgccagat gggatcggga
     8761 tgcacacggg aaaatcttga aagcataatt acggacttcc tgaaccacct gggaattgat
     8821 ttcaagatcg taggcgattc ctgcatggtc tatggggata cccttgatgt aatgcacgga
     8881 gacctggaac tttcctctgc agtagtcgga cccataccgc ttgaccggga atggggtatt
     8941 gataaaccct ggataggggc aggtttcggg ctcgaacgcc ttctaaaggt taaacacgac
     9001 tttaaaaata tcaagagagc tgcaaggtcc gagtcttact ataacgggat ttctaccaac
     9061 ctgtaaccta ggctgctgcc accgctgaga tctatggccc gcgcccacag gaaggagcta
     9121 ccggcagcgg tgcggactgt tgtaactcat aataagaaat gaggccgctc atggcgttct
     9181 gttgcccgtc tcgctggtga aaagaaaaac caccctggct aactaagcgg cctgctgact
     9241 ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt
     9301 aagatgcgcc ccgcattgga aacctgatca tgtagatcga atggactcta aatccgttca
     9361 gccgggttag attcccgggg tttccgccaa attcgaaaag cctgctcaac gagcaggctt
     9421 ttttgcatgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagatccc
     9481 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgc
     9541 gtttaaaccc atggacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     9601 tacgactcag ggcccactag tggtacccgg ggatcctcta gagtcgacct gcaggtcgac
     9661 ggatccgggg aattcaggct tggaggatac gtatgactaa aaaaatttca ttcattatta
     9721 acggccaggt tgaaatcttt cccgaaggtg atgatttagt gcaatccatt aattttggtg
     9781 ataatagtgt ttacctgcca atattgaatg actctcatgt aaaaaacatt attgattgta
     9841 atggaaataa cgaattacgg ttgcataaca ttgtcaattt tctctatacg gtagggcaaa
     9901 gatggaaaaa tgaagaatac tcaagacgca ggacatacat tcgtgactta aaaaaatata
     9961 tgggatattc agaagaaatg gctaagctag aggccaattg gata
//
