LOCUS       sequence-338862-        4891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EarlyBac insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Chou TH, Tajima N, Romero-Hernandez A, Furukawa H
  TITLE     Structural Basis of Functional Transitions in Mammalian NMDA 
            Receptors.
  JOURNAL   Cell. 2020 Jul 23;182(2):357-371.e13. doi: 
            10.1016/j.cell.2020.05.052. Epub 2020 Jun 30.
  PUBMED    32610085
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.05.052"; journalName: "Cell"; date: 
            "2020-07-23- 23"; volume: "182"; issue: "2"; pages: "357-371.e13"
COMMENT     Sequence Label: pUCDMp10
FEATURES             Location/Qualifiers
     source          1..4891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..365
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(418..451)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    641..689
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     714..733
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(825..934)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        1827..2279
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2226..2247
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     2293..2312
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2293..2311
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3194..3328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3231..3250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3285..3304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3743..3845
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             3846..4505
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3912..3931)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattcccatg tcagccgtta agtgttcctg tgtcactcaa aattgctttg agaggctcta
       61 agggcttctc agtgcgttac atccctggct tgttgtccac aaccgttaaa ccttaaaagc
      121 tttaaaagcc ttatatattc ttttttttct tataaaactt aaaaccttag aggctattta
      181 agttgctgat ttatattaat tttattgttc aaacatgaga gcttagtacg tgaaacatga
      241 gagcttagta cgttagccat gagagcttag tacgttagcc atgagggttt agttcgttaa
      301 acatgagagc ttagtacgtt aaacatgaga gcttagtacg tgaaacatga gagcttagta
      361 cgtactatca acaggttgaa ctgctgatca acagatcctc tacgcggccg cggtaccata
      421 acttcgtata gcatacatta tacgaagtta tctggtttaa acgtacccgt agtggctatg
      481 gcagggcttg ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggt
      541 tggggtgggg aaaaggaaga aacgcgggcg tattggtccc aatggggtct cggtggggta
      601 tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc
      661 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
      721 cttccgtgtt tcagttagcc tcccccatct cccggtaccg catgctatgc atcagctgct
      781 agcaccatgg ctcgagatcc cgggtgatca agtcttcgtc gagtgattgt aaataaaatg
      841 taatttacag tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa
      901 ctataatata ttgtgttggg ttgaattaaa ggtccgtata ctagtatcga tgattgaccc
      961 caacaaaaga tttataatta atcataatca cgaacaacaa caagtcaatg aaacaaataa
     1021 acaagttgtc gataaaacat tcataaatga cacagcaaca tacaattctt gcataataaa
     1081 aatttaaatg acatcatatt tgagaataac aaatgacatt atccctcgat tgtgttttac
     1141 aagtagaatt ctacccgtaa agcgagttta gttttgaaaa acaaatgaca tcatttgtat
     1201 aatgacatca tcccctgatt gtgttttaca agtagaattc tatccgtaaa gcgagttcag
     1261 ttttgaaaac aaatgagtca tacctaaaca cgttaataat cttctgatat cagcttatga
     1321 ctcaagttat gagccgtgtg caaaacatga gataagttta tgacatcatc cactgatcgt
     1381 gcgttacaag tagaattcta ctcgtaaagc cagttcggtt atgagccgtg tgcaaaacat
     1441 gacatcagct tatgactcat acttgattgt gttttacgcg tagaattcta ctcgtaaagc
     1501 gagttcggtt atgagccgtg tgcaaaacat gacatcagct tatgagtcat aattaatcgt
     1561 gcgttacaag tagaattcta ctcgtaaagc gagttgaagg atcatattta gttgcgttta
     1621 tgagataaga ttgaaaagcg tgtaaaatgt ttcccgcgct tggcacaact atttacaatg
     1681 cggccaagtt ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt
     1741 gtttgcgtac gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa
     1801 ccctagtatt ggagcaataa tcgatcctag aatcccaaaa caaactggtt attgtggtag
     1861 gtcatttgtt tggcagaaag aaaactcgag aaatttctct ggccgttatt cgttattctc
     1921 tcttttcttt ttgggtctct ccctctctgc actaatgctc tctcactctg tcacacagta
     1981 aacggcatac tgctctcgtt ggttcgagag agcgcgcctc gaatgttcgc gaaaagagcg
     2041 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
     2101 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
     2161 atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat
     2221 caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag agaactctga
     2281 atagggaatt ggtaatacga ctcactatag ggggatcccg gtccgaagcg cgcggaattc
     2341 aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga atgaatcgtt
     2401 tttaaaataa caaatcaatt gttttataat attcgtacga ttctttgatt atgtaataaa
     2461 atgtgatcat taggaagatt acgaaaaata taaaaaatat gagttctgtg tgtataacaa
     2521 atgctgtaaa cgccacaatt gtgtttgttg caaataaacc catgattatt tgattaaaat
     2581 tgttgttttc tttgttcata gacaatagtg tgttttgcct aaacgtgtac tgcataaact
     2641 ccatgcgagt gtatagcgag ctagtggcta acgcttgccc caccaaagta gattcgtcaa
     2701 aatcctcaat ttcatcaccc tcctccaagt ttaacatttg gccgtcggaa ttaacttcta
     2761 aagatgccac ataatctaat aaatgaaata gagattcaaa cgtggcgtca tcgtccgttt
     2821 cgaccatttc cgaaaagaac tcgggcataa actctatgat ttctctggac gtggtgttgt
     2881 cgaaactctc aaagtacgca gtcaggaacg tgcgcgacat gtcgtcggga aactcgcgcg
     2941 gaaacatgtt gttgtaaccg aacgggtccc atagcgccaa aaccaaatct gccagcgtca
     3001 atagaatgag cacgatgccg acaatggagc tggcttggat agcgattcga gttaacggcc
     3061 ggccaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
     3121 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3181 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3241 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3301 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg
     3361 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat
     3421 aacctaaccc cttggggcct ctaaacgggt cttgaggggt tttttgaccc taggggcctc
     3481 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat ctgaacagga
     3541 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     3601 tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag
     3661 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     3721 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     3781 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     3841 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     3901 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     3961 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     4021 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     4081 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     4141 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     4201 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     4261 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     4321 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     4381 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     4441 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     4501 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggttgctac gcctgaataa
     4561 gtgataataa gcggatgaat ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc
     4621 agggcagggt cgttaaatag ccgcttatgt ctattgctgg tttaccggtt tattgactac
     4681 cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
     4741 cgtggaggta ataattgacg atatgatcat ttattctgcc tcccagctga cattcatccg
     4801 gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     4861 gcgccctgag tgcttgcggc agcgtgaagc t
//
