LOCUS       sequence-338863-        5784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  bacteria expression plasmid for a UDP-GlcNAc sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Li Z, Zhang J, Ai HW
  TITLE     Genetically Encoded Green Fluorescent Biosensors for Monitoring 
            UDP-GlcNAc in Live Cells.
  JOURNAL   ACS Cent Sci. 2021 Oct 27;7(10):1763-1770. doi: 
            10.1021/acscentsci.1c00745. Epub 2021 Sep 30.
  PUBMED    34729420
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.1c00745"; journalName: "ACS Cent Sci"; date: 
            "2021-10-27- 27"; volume: "7"; issue: "10"; pages: "1763-1770"
COMMENT     Sequence Label: pBad-UGAcS
FEATURES             Location/Qualifiers
     source          1..5784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     729..750
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(862..883)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2185..2202)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2355..2441
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2533..2560
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        2579..2670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2671..2739,2740..3531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2889..2908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3702..4290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4191..4210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4444..4461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4476..4616
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4542..4561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4702..4724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4880..5758)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggctcgagt atgagtggtc aaggaaagcg attaatggtg
      361 atagcaggcg gaaccggtgg atctgtattc ccgggactgg cggttgcgca ccatctaatg
      421 gctcagggtt ggcaagttcg ctggctggga actgccgacc gtatggaagc ggacttagtg
      481 ccaaaacatg gcatcgaaat tgatttcatt cgtatctctg gtgaggttta tatcaaggcc
      541 gacaagcaga agaacggcat caaggcgaac ttccagatcc gccacaacgt ggaggacggc
      601 agcatgcagc tcgccgacca ctaccagcag aacaccccca ttggcgacgg ccccgtgctg
      661 ctgcccgaca accattacct gagcacccag tccgtgctga gcaaagaccc caacgagaag
      721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
      781 gagctgcaca aggtggacgg cggatccggt ggcaccggag tgagcagggg cgaggagctg
      841 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      901 cgcgtgaggg gcgaaggcga gggcgatgcc accaacggca agctgaccct gaagttcatc
      961 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc
     1021 gtgcagtgct tctcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1081 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg cacctacaag
     1141 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1201 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacga gtctctgcgt
     1261 ggaaaaggta taaaagcact gatagctgcc ccgctgcgta tcttcaacgc ctgggagcag
     1321 gcgcgggcga ttatgaaagc gtacaaacct gacgtggtgc tcggtatggg aggctacgtg
     1381 tcaggtccag gtggtctggc cgcgtggtcg ttaggcattc cggttgtact tcatgaacaa
     1441 aacggtattg cgggcttaac caataaatgg ctggcgaaga ttgccaccaa agtgatgcag
     1501 gcgtttccag gtgctttccc taatgcggaa gtagtgggta acccggtgcg taccgatgtg
     1561 ttggcgctgc cgttgccgcg gcaacgtttg gctggacgtg aaggtccggt tcgtgtgctg
     1621 gtagtgggtg gtacgacggg cgcacgcatt cttaaccaga caatgccgca ggttgctgcg
     1681 aaactgggtg attcagtcac tatctggcat cagagcggca aaggttcgca acaatccgtt
     1741 gaacaggcgt atgccgaagc agggcaacca cagcataagg tgacgggatt tattgatgat
     1801 atggcggcgg cgtatgcgtg ggcggatgtc gtcgtttgcc gctccggtgc gttaacggtg
     1861 agtgaaatcg ccgcggcagg actaccggcg ttgtttgtgc cgtttcaaca taaagaccgc
     1921 cagcaatact ggaatgcgct accgctggaa aaagcgggcg cagccaaaat tatcgagcag
     1981 ccacagctta gcgtggatgc tgtcgccaac accctggccg ggtggtcgcg agaaacctta
     2041 ttaaccatgg cagaacgcgc ccgtgctgca tccattccgg atgccaccga gcgagtggca
     2101 aatgaagtga gccgggttgc ccgggcgcac caacaccacc atcattaagc ttggctgttt
     2161 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     2221 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
     2281 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
     2341 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2401 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
     2461 acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
     2521 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt
     2581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2881 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     2941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     3001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     3061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     3121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     3181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3361 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3841 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4321 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     4381 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4441 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     4501 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     4561 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     4621 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4681 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4741 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg
     4801 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa
     4861 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac
     4921 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat
     4981 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc
     5041 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc
     5101 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga
     5161 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc
     5221 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta
     5281 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg
     5341 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa
     5401 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg
     5461 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat
     5521 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct
     5581 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca
     5641 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca
     5701 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac
     5761 ttttcatact cccgccattc agag
//
