LOCUS       sequence-33887-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Patterson-Fortin J, Shao G, Bretscher H, Messick TE, Greenberg RA
  TITLE     Differential regulation of JAMM domain deubiquitinating enzyme 
            activity within the RAP80 complex.
  JOURNAL   J Biol Chem. 2010 Oct 1. 285(40):30971-81.
  PUBMED    20656689
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Oct 1. 285(40):30971-81."
COMMENT     Sequence Label: pOZ-N-FH MERIT40
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             76..102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atcctctaga ctgccagatc ttccgctcga tgaaccatgg actacaagga cgacgatgac
       61 aagctcgatg gaggataccc ctacgacgtg cccgactacg ccggaggact cgaggaagtg
      121 gcagagccca gcagccccac tgaagaggag gaggaggaag aggagcactc ggcagagcct
      181 cggccccgca ctcgctccaa tcctgaaggg gctgaggacc gggcagtagg ggcacaggcc
      241 agcgtgggca gccgcagcga gggtgagggt gaggccgcca gtgctgatga tgggagcctc
      301 aacacttcag gagccggccc taagtcctgg caggtgcccc cgccagcccc tgaggtccaa
      361 attcggacac caagggtcaa ctgtccagag aaagtgatta tctgcctgga cctgtcagag
      421 gaaatgtcac tgccaaagct ggagtcgttc aacggctcca aaaccaacgc cctcaatgtc
      481 tcccagaaga tgattgagat gttcgtgcgg acaaaacaca agatcgacaa aagccacgag
      541 tttgcactgg tggtggtgaa cgatgacacg gcctggctgt ctggcctgac ctccgacccc
      601 cgcgagctct gtagctgcct ctatgatctg gagacggcct cctgttccac cttcaatctg
      661 gaaggacttt tcagcctcat ccagcagaaa actgagcttc cggtcacaga gaacgtgcag
      721 acgattcccc cgccatatgt
//
