LOCUS       sequence-338891-        9447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of murine NFAT (aa 4-462) fused to the 
            DNA-binding domain from TALEN1257, the VP64 activation domain and 
            the membrane anchor peptide KRɸ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Mesko M, Lebar T, Dekleva P, Jerala R, Bencina M
  TITLE     Engineering and Rewiring of a Calcium-Dependent Signaling Pathway.
  JOURNAL   ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 
            10.1021/acssynbio.0c00133. Epub 2020 Jul 20.
  PUBMED    32643923
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00133"; journalName: "ACS Synth Biol"; date: 
            "2020-08-21- 21"; volume: "9"; issue: "8"; pages: "2055-2065"
COMMENT     Sequence Label: mNFAT4-462:TALE:VP64:KRɸ
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        208..587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        588..791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     742..762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     836..855
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        836..854
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      917..926
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             926..955
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4712..4861
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        complement(4973..4991)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4974..4991)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5011..5028)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5017..5241
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5287..5715
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5374..5393)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5584..5605
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5724..5744)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5729..6058
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5909..6044
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5971..5990
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6125..6919
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6179..6198)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6789..6808
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7093..7214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7130..7149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7184..7203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7263..7279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7263..7279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7276..7298)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7287..7303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7311..7317,7318..7335,7336..7341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7356..7377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7494..7511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7665..8253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7745..7764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8424..9215,9216..9284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9047..9066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9285..9389)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
       61 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      181 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      601 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      721 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      781 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
      841 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      901 cagtgtgctg gaattcgcca ccatggagca gaagctgatc agcgaggagg acctgccgga
      961 gccgcagccc gaccccgatg gcggggacgg ccccggccac gagcccgggg gcagtcccca
     1021 agacgagctg gacttttcca tcctcttcga ttatgactat ctgaacccta tcgaagaaga
     1081 accgatcgca cataaggcca tcagctcacc ctccggactc gcatacccgg atgatgtcct
     1141 ggactatggc ctcaagccat gcaaccccct tgccagtctc tctggcgagc cccctggccg
     1201 gttcggagag ccggatagta tagggttcca gaactttctg agcccggtca agccagcagg
     1261 ggcttcgggc ccgagccctc ggatcgagat cactccatcc cacgaactga tgcaggcagg
     1321 gggggccctc cgtgggagag acgccggcct gtcccccgag cagccggccc tggccctggc
     1381 cggcgtggcc gccagcccga ggttcacact gcccgtgccc ggctacgagg gctaccgcga
     1441 gccgctttgc ttgagccccg ctagcagcgg ctcctctgcc agcttcattt ctgacacctt
     1501 ctccccctac acctcgccct gcgtctcacc caataacgcc gggcccgacg acctgtgtcc
     1561 ccagtttcaa aacatccctg ctcattattc ccccagaacc tctccaataa tgtcacctcg
     1621 aaccagcctc gccgaggaca gctgcctggg ccgacactcg cccgtgcccc gtccggcatc
     1681 ccgctcctcc tcacccggtg ccaagcggag gcattcgtgc gcagaggctt tggttgctcc
     1741 tctgcccgca gcctcacccc agcgctcccg gagcccctcg ccacagccct cgcctcacgt
     1801 ggcaccgcag gacgacagca tccccgctgg gtaccccccc acggccggct ctgctgttct
     1861 catggatgcc ctcaacaccc tggccaccga ctcgccctgc gggatcccct ccaagatatg
     1921 gaagaccagt cctgacccga cgcctgtgtc caccgctccg tccaaggctg gcctggcccg
     1981 ccacatctac cctactgtgg agttcctggg gccatgtgag caggaggaga ggaggaattc
     2041 cgctccagag tccatcctgc tggtaccacc tacttggccc aagcagttgg tgccggccat
     2101 tcccatctgc agcatccctg tgactgcatc cctcccacca ctcgagtggc cactctccaa
     2161 tcagtcgggc tcctatgagc tacggattga ggtccaaccc aagccccatc accgggccca
     2221 ctatgagacg gagggcagcc gtggcgctgt caaagcccca acaggaggac accctgtggt
     2281 gcagctccac ggctacatgg agaacaagcc tctggggctt cagatccaga tcggcggctc
     2341 tggcggcggc tccggaggct ctgtggactt gaggacactc ggttattcgc aacagcaaca
     2401 ggagaaaatc aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg cgcttgtggg
     2461 gcatggcttc actcatgcgc atattgtcgc gctttcacag caccctgcgg cgcttgggac
     2521 ggtggctgtc aaataccaag atatgattgc ggccctgccc gaagccacgc acgaggcaat
     2581 tgtaggggtc ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc tgactgtggc
     2641 gggtgagctt agggggcctc cgctccagct cgacaccggg cagctgctga agatcgcgaa
     2701 gagaggggga gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc tcaccggggc
     2761 ccccttgaac ctgaccccag accaggtagt cgcaatcgcg tcaaacggag ggggaaagca
     2821 agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg gccttacacc
     2881 ggagcaagtc gtggccattg caagcaatgg gggtggcaaa caggctcttg agacggttca
     2941 gagacttctc ccagttctct gtcaagccca cgggctgact cccgatcaag ttgtagcgat
     3001 tgcgtcgaac attggaggga aacaagcatt ggagactgtc caacggctcc ttcccgtgtt
     3061 gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc atcgccagcc atgatggcgg
     3121 taagcaggcg ctggaaacag tacagcgcct gctgcctgta ctgtgccagg atcatgtact
     3181 gaccccagac caggtagtcg caatcgcgtc aaacggaggg ggaaagcaag ccctggaaac
     3241 cgtgcaaagg ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt
     3301 ggccattgca aataataacg gtggcaaaca ggctcttgag acggttcaga gacttctccc
     3361 agttctctgt caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgtcgcatga
     3421 cggagggaaa caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca
     3481 cggtttgacg cctgcacaag tggtcgccat cgcctcgaat ggcggcggta agcaggcgct
     3541 ggaaacagta cagcgcctgc tgcctgtact gtgccaggat catggactga ccccagacca
     3601 ggtagtcgca atcgcgaaca ataatggggg aaagcaagcc ctggaaaccg tgcaaaggtt
     3661 gttgccggtc ctttgtcaag accacggcct tacaccggag caagtcgtgg ccattgcatc
     3721 ccacgacggt ggcaaacagg ctcttgagac ggttcagaga cttctcccag ttctctgtca
     3781 agcccacggg ctgactcccg atcaagttgt agcgattgcg tccaacggtg gagggaaaca
     3841 agcattggag actgtccaac ggctccttcc cgtgttgtgt caagcccacg gtttgacgcc
     3901 tgcacaagtg gtcgccatcg ccagccatga tggcggtaag caggcgctgg aaacagtaca
     3961 gcgcctgctg cctgtactgt gccaggatca tggactgacc ccagaccagg tagtcgcaat
     4021 cgcgtcacat gacgggggaa agcaagccct ggaaaccgtg caaaggttgt tgccggtcct
     4081 ttgtcaagac cacggcctta caccggagca agtcgtggcc attgcatccc acgacggtgg
     4141 caaacaggct cttgagacgg ttcagagact tctcccagtt ctctgtcaag cccacgggct
     4201 gactcccgat caagttgtag cgattgcgaa taacaatgga gggaaacaag cattggagac
     4261 tgtccaacgg ctccttcccg tgttgtgtca agcccacggt ttgacgcctg cacaagtggt
     4321 cgccatcgcc agccatgatg gcggtaagca ggcgctggaa acagtacagc gcctgctgcc
     4381 tgtactgtgc caggatcatg gactgacacc cgaacaggtg gtcgccattg cttctaatgg
     4441 gggaggacgg ccagccttgg agtccatcgt agcccaattg tccaggcccg atcccgcgtt
     4501 ggctgcgtta acgaatgacc atctggtggc gttggcatgt cttggtggac gacccgcgct
     4561 cgatgcagtc aaaaagggtc tgcctcatgc tcccgcattg atcaaaagaa ccaaccggcg
     4621 gattcccgag agaacttccc atcgagtcgc gggatccggc ggctctggcg gcggctccgg
     4681 aggctctgag gccagcggtt cgggacgggc tgacgcattg gacgattttg atctggatat
     4741 gctgggaagt gacgccctcg atgattttga ccttgacatg cttggttcgg atgcccttga
     4801 tgactttgac ctcgacatgc tcggcagtga cgcccttgat gatttcgacc tggacatgct
     4861 gattaacact agtggcggct ctggcggcgg ctccggaggc tctggcaaga agttctggaa
     4921 gaggctgagg aagttcctga ggaagctgaa gagctaatct agagggccct attctatagt
     4981 gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     5041 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     5101 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     5161 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     5221 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     5281 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     5341 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     5401 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     5461 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     5521 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5581 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     5641 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     5701 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5761 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     5821 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5881 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5941 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     6001 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     6061 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     6121 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     6181 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     6241 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     6301 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     6361 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     6421 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     6481 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     6541 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     6601 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     6661 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     6721 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     6781 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6841 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6901 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6961 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7021 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7081 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7141 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7201 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7261 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7321 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7381 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7441 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8101 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9421 gacggatcgg gagatctccc gatcccc
//
