LOCUS       sequence-338893-        7577 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTPIP51(1-470) in human HeLa cells after transfection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pCAG-FLAG-puro-PTPIP51(1-470)
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          1..1017
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     941..963
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1025..1044
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1072..1081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1081..1104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(2547..3121,3122..3124,3125..3133)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2714..2731)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2941..2960
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3134..3733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3134..3153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3630..3650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3814..3831)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3820..4044
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4091..4107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4091..4107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4104..4126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4115..4131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4139..4145,4146..4163,4164..4169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4184..4205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4264..4459
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4310..4445
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4465..4599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4667..4684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4838..5426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4918..4937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5597..6388,6389..6457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6220..6239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     polyA_signal    7050..7184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        7198..7577
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
       61 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      121 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      181 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      241 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      301 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
      361 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
      421 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
      481 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
      541 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
      601 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
      661 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
      721 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
      781 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
      841 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
      901 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
      961 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1021 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt agccaccatg
     1081 gattacaagg atgacgacga taagctcgag atgtctagac tgggagccct gggtggtgcc
     1141 cgtgccgggc tgggactgtt gctgggtacc gccgccggcc ttggattcct gtgcctcctt
     1201 tacagccagc gatggaaacg gacccagcgt catggccgca gccagagcct gcccaactcc
     1261 ctggactata cgcagacttc agatcccgga cgccacgtga tgctcctgcg ggctgtccca
     1321 ggtggggctg gagatgcctc agtgctgccc agccttccac gggaaggaca ggagaaggtg
     1381 ctggaccgcc tggactttgt gctgaccagc cttgtggcgc tgcggcggga ggtggaggag
     1441 ctgagaagca gcctgcgagg gcttgcgggg gagattgttg gggaggtccg atgccacatg
     1501 gaagagaacc agagagtggc tcggcggcga aggtttccgt ttgtccggga gaggagtgac
     1561 tccactggct ccagctctgt ctacttcacg gcctcctcgg gagccacgtt cacagatgct
     1621 gagagtgaag ggggttacac aacagccaat gcggagtctg acaatgagcg ggactctgac
     1681 aaagaaagtg aggacgggga agatgaagtg agctgtgaga ctgtgaagat ggggagaaag
     1741 gattctcttg acttggagga agaggcagct tcaggtgcct ccagtgccct ggaggctgga
     1801 ggttcctcag gcttggagga tgtgctgccc ctcctgcagc aggccgacga gctgcacagg
     1861 ggtgatgagc aaggcaagcg ggagggcttc cagctgctgc tcaacaacaa gctggtgtat
     1921 ggaagccggc aggactttct ctggcgcctg gcccgagcct acagtgacat gtgtgagctc
     1981 actgaggagg tgagcgagaa gaagtcatat gccctagatg gaaaagaaga agcagaggct
     2041 gctctggaga agggggatga gagtgctgac tgtcacctgt ggtatgcggt gctttgtggt
     2101 cagctggctg agcatgagag catccagagg cgcatccaga gtggctttag cttcaaggag
     2161 catgtggaca aagccattgc tctccagcca gaaaacccca tggctcactt tcttcttggc
     2221 aggtggtgct atcaggtctc tcacctgagc tggctagaaa aaaaaactgc tacagccttg
     2281 cttgaaagcc ctctcagtgc cactgtggaa gatgccctcc agagcttcct aaaggctgaa
     2341 gaactacagc caggattttc caaagcagga agggtatata tttccaagtg ctacagagaa
     2401 ctagggaaaa actctgaagc tagatggtgg atgaagttgg ccctggagct gccagatgtc
     2461 acgaaggagg atttggctat ccagaaggac ctggaagaac tggaagtcat tttacgagac
     2521 taggcggccg cgaattccgc cccccccccc tctccctccc ccccccctaa cgttactggc
     2581 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     2641 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     2701 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     2761 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     2821 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     2881 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     2941 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     3001 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     3061 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     3121 tatggccaca accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3721 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     3781 ccatgcatcg atgatctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3841 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3901 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3961 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4021 ctggggatgc ggtgggctct atggcttctg aggcggaaag aacctgcagc ccaagcttgg
     4081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     4261 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4321 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4381 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4441 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     4501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4561 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
     4621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgaccgc
     6481 tttggaaaaa caaagactgt atttcctgga aattaatgtt tattcaataa actgtgtatt
     6541 cagctatatt cacatagtgg tgaggctgaa atgaggcggg aagaggcggt tggggcttaa
     6601 ttatatcaat ttgggtggcc ccacagcgcc tccaaggcgc cagtcctgtt ttgacaagtt
     6661 gcctctggaa gcctctcttc tttttctcca gagtaagcgg aggccagggg cccccggcct
     6721 ctgcttaata ctaaaaaaaa cagctgttgt catagtaatg attgggtgga aacattccag
     6781 gcctgggtgg agaggctttt tgcttcctct tgcaaaacca cactgacatt ccaggcctgg
     6841 gtggagaggc tttttgcttc ctcttgcaaa accacactgc cctctggagg gcagttgcct
     6901 agcaactaat taaaagagga tgtcgcacgg ccagctgcgg tcagttagtc acttcctgct
     6961 taactgactt gacattttct attttaagag tcgggaggaa aattactgtg ttggaggccc
     7021 tccgccatct tctgaagctg aatcgaatta acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgatat catcgtcgac
     7201 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     7261 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     7321 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     7381 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     7441 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     7501 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     7561 tcatcgctat taccatg
//
