LOCUS       sequence-338899-       10112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for
            Drosophila transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10112)
  AUTHORS   Jauregui-Lozano J, Bakhle K, Weake VM
  TITLE     In vivo tissue-specific chromatin profiling in Drosophila 
            melanogaster using GFP-tagged nuclei.
  JOURNAL   Genetics. 2021 May 22. pii: 6281219. doi: 10.1093/genetics/iyab079.
  PUBMED    34022041
REFERENCE   2  (bases 1 to 10112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 May 22. pii: 6281219. doi: 10.1093/genetics/iyab079."
COMMENT     Sequence Label: pUASTattB-GFP-Msp300KASH
FEATURES             Location/Qualifiers
     source          1..10112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(759..778)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     878..900
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(938..956)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    1203..1272
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        1343..1447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1448..1516,1517..2308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1666..1685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2479..3067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2968..2987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(3302..3332,3333..3887)
                     /label=P element 5' end
     misc_feature    join(4743..4773,4774..4975)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    5037..5131
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5150..5388
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5335..5356
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(5458..5486)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6081..6101
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     intron          6420..6485
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6615..6635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6907..7041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6944..6963)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6998..7017
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7068..7086)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(7538..7609,8011..8284,8359..9013,9075..9390,9594..9725,
                     9796..10112,1..298)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 cttcttcttg aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg
       61 gttccgttac gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga
      121 aattagctgc acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac
      181 gcttaacttc tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt
      241 gagcttccgg gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc
      301 gacatatatc cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac
      361 tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat
      421 gacctataga atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt
      481 cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac
      541 ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata
      601 attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc
      661 gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt
      721 cggtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
      781 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      841 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      901 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      961 taggttaatg tcatgataat aatggtttct tagacgtcaa ttcggcttgt cgacatgccc
     1021 gccgtgaccg tcgagaaccc gctgacgctg ccccgcgtat ccgcacccgc cgacgccgtc
     1081 gcacgtcccg tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga gggcttcccg
     1141 gtgcgccgcg cgttcgccgg gatcaactac cgccacctcg acccgttcat catgatggac
     1201 cagatgggtg aggtggagta cgcgcccggg gagcccaagg gcacgccctg gcacccgcac
     1261 cgcggcttcg agaccgtgac ctacatcgtc gacggtacct gaagccgaat tgacgtcagg
     1321 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1381 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1441 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     1501 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1561 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1621 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1681 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1741 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1801 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1861 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1921 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1981 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2041 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2101 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2161 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2221 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2281 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     2341 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     2401 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     2461 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2521 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2581 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2641 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2701 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2761 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2821 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2881 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2941 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3001 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3061 atggaaaaac gccttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc
     3121 gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt
     3181 ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt
     3241 catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc
     3301 ccatgatgaa ataacataag gtggtcccgt cgatagccga agcttaccga agtatacact
     3361 taaattcagt gcacgtttgc ttgttgagag gaaaggttgt gtgcggacga attttttttt
     3421 gaaaacatta acccttacgt ggaataaaaa aaaatgaaat attgcaaatt ttgctgcaaa
     3481 gctgtgactg gagtaaaatt aattcacgtg ccgaagtgtg ctattaagag aaaattgtgg
     3541 gagcagagcc ttgggtgcag ccttggtgaa aactcccaaa tttgtgatac ccactttaat
     3601 gattcgcagt ggaaggctgc acctgcaaaa ggtcagacat ttaaaaggag gcgactcaac
     3661 gcagatgccg tacctagtaa agtgatagag cctgaaccag aaaagataaa agaaggctat
     3721 accagtggga gtacacaaac agagtaagtt tgaatagtaa aaaaaatcat ttatgtaaac
     3781 aataacgtga ctgtgcgtta ggtcctgttc attgtttaat gaaaataaga gcttgaggga
     3841 aaaaattcgt actttggagt acgaaatgcg tcgtttagag cagcagccga attaattcta
     3901 gttccagtga aatccaagca ttttctaaat taaatgtatt cttattatta tagttgttat
     3961 ttttgatata tataaacaac actattatgc ccaccatttt tttgagatgc atctacacaa
     4021 ggaacaaaca ctggatgtca ctttcagttc aaattgtaac gctaatcact ccgaacaggt
     4081 cacaaaaaat taccttaaaa agtcataata ttaaattaga ataaatatag ctgtgaggga
     4141 aatatataca aatatattgg agcaaataaa ttgtacatac aaatatttat tactaatttc
     4201 tattgagacg aaatgaacca ctcggaacca tttgagcgaa ccgaatcgcg cggaactaac
     4261 gacagtcgct ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga
     4321 cagcgaaaga gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg
     4381 atttgaaaag tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt
     4441 tttgatgtaa ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac
     4501 ttgtggggat gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg
     4561 gcacaaatat aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat
     4621 aatggtaaaa aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg
     4681 ggcccgaagt cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag
     4741 accatgatga aataacataa ggtggtcccg tcggcaagag acatccactt aacgtatgct
     4801 tgcaataagt gcgagtgaaa ggaatagtat tctgagtgtc gtattgagtc tgagtgagac
     4861 agcgatatga ttgttgatta acccttagca tgtccgtggg gtttgaatta actcataata
     4921 ttaattagac gaaattattt ttaaagtttt atttttaata atttgcgagt acgcaaagct
     4981 tggctgcatc caacgcgttg ggagctctcc ggatccaagc ttgcatgcct gcaggtcgga
     5041 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     5101 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     5161 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     5221 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     5281 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     5341 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     5401 ggaattcgtt aacagatctg cggccgcatg agtaaaggag gagaactttt cactggagtt
     5461 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     5521 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     5581 aaactacctg ttccatggcc aaccctggtc accaccctga cctacggcgt gcagtgcttc
     5641 tcccgttacc ctgatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga
     5701 tacgtgcagg agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa
     5761 gtcaagtttg agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag
     5821 gaggacggaa acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac
     5881 atcatggccg acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc
     5941 gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc
     6001 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     6061 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     6121 ggcatggatg aactatacaa agctgctgct gctgctgctg ggcgcgccat gcgcgttgca
     6181 cgtgcctcgc tgcccattca ggcgctcatg ctgctgcttt taggtgtggc caccctggtg
     6241 ccccacggtg aggactacac ctgtatgttc tccaacacct ttgcgcgcag cctggagccc
     6301 atgttgtcgt atccccacgg accgccaccc acgtaatcta gaggatcttt gtgaaggaac
     6361 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     6421 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     6481 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     6541 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     6601 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     6661 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     6721 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     6781 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     6841 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     6901 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7021 gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg taagttaata
     7081 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     7141 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     7201 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     7261 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     7321 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     7381 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     7441 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     7501 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     7561 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     7621 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     7681 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     7741 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     7801 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     7861 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     7921 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     7981 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     8041 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     8101 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     8161 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     8221 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     8281 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     8341 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     8401 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     8461 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     8521 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     8581 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     8641 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     8701 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     8761 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     8821 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     8881 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     8941 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     9001 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     9061 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     9121 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     9181 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     9241 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     9301 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     9361 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     9421 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     9481 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     9541 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     9601 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     9661 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     9721 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     9781 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     9841 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     9901 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     9961 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
    10021 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
    10081 ccggttatca taccattcct gctctttggc gg
//
