LOCUS       sequence-338904-       10160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Msp300 KASH domain fused to 2xFLAG and mCherry under 
            Gal4/UAS control for Drosophila transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Jauregui-Lozano J, Bakhle K, Weake VM
  TITLE     In vivo tissue-specific chromatin profiling in Drosophila 
            melanogaster using GFP-tagged nuclei.
  JOURNAL   Genetics. 2021 May 22. pii: 6281219. doi: 10.1093/genetics/iyab079.
  PUBMED    34022041
REFERENCE   2  (bases 1 to 10160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2021 May 22. pii: 6281219. doi: 10.1093/genetics/iyab079."
COMMENT     Sequence Label: pUASTattB-2xFlag-mCherry-Msp300KASH
FEATURES             Location/Qualifiers
     source          1..10160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          258..323
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             453..473
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    745..879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(782..801)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     836..855
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(906..924)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1376..1447,1849..2122,2197..2851,2913..3228,3432..3563,
                     3634..4248)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     complement(4709..4728)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4828..4850
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4888..4906)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    5153..5222
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        5293..5397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5398..5466,5467..6258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5616..5635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6429..7017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6918..6937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(7252..7282,7283..7837)
                     /label=P element 5' end
     misc_feature    join(8693..8723,8724..8925)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    8987..9081
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        9100..9338
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     9285..9306
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             9405..9428
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             9435..10139
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(9577..9595)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9845..9864
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgcgccatgc gcgttgcacg tgcctcgctg cccattcagg cgctcatgct gctgctttta
       61 ggtgtggcca ccctggtgcc ccacggtgag gactacacct gtatgttctc caacaccttt
      121 gcgcgcagcc tggagcccat gttgtcgtat ccccacggac cgccacccac gtaatctaga
      181 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
      241 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
      301 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
      361 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
      421 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
      481 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
      541 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
      601 ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
      661 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      721 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      781 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
      841 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc actagaaggc ctaattctag
      901 tatgtatgta agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt
      961 tttttactgc actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca
     1021 agggtatttg atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca
     1081 aagtcagcgc tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct
     1141 gcgtccgcta tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca
     1201 gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac
     1261 acaaagtgct gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg
     1321 gggtggtgat tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg
     1381 ccaagaggat caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct
     1441 gaacaatgtg agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg
     1501 attgcaacat caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca
     1561 attgcacaaa caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa
     1621 gacaattttt gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat
     1681 tcttttttac aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa
     1741 taaataaaag tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag
     1801 atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga
     1861 gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc
     1921 cggccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc
     1981 acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg
     2041 cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc
     2101 aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat
     2161 cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg
     2221 aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg
     2281 cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc
     2341 aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct
     2401 ttatcggctc cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac
     2461 gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc
     2521 tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg
     2581 gagaaaggaa gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct
     2641 gcgatgagcc cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga
     2701 agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc
     2761 tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca
     2821 ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc
     2881 tagtattaca taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac
     2941 aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc
     3001 cgtgatcgga tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg
     3061 gagcagttgt tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac
     3121 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     3181 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     3241 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat
     3301 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     3361 ttgcagaaga taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt
     3421 ttaatttgca gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc
     3481 aagtgggtgt gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc
     3541 aaaacgtctt tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat
     3601 ttactaactt tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt
     3661 atgagggagg cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt
     3721 gccgaattgc cgctttttct cacagtgcca ctggtcttca cggcgattgc ctatccgatg
     3781 atcggactgc gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg
     3841 gccaatgtgt caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg
     3901 gcgctgtctg tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg
     3961 aactcgggct cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac
     4021 gccaacgagg gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc
     4081 acatcgtcga acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc
     4141 tccgccgccg atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg
     4201 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc
     4261 cgaaataact gcttgttttt tttttttacc attattacca tcgtgtttac tgtttattgc
     4321 cccctcaaaa agctaatgta attatatttg tgccaataaa aacaagatat gacctataga
     4381 atacaagtat ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca
     4441 aaagacttac acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc
     4501 gggatatatt ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc
     4561 accacaccac gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt
     4621 caccttttgt tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta
     4681 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4741 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4801 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4861 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4921 tcatgataat aatggtttct tagacgtcaa ttcggcttgt cgacatgccc gccgtgaccg
     4981 tcgagaaccc gctgacgctg ccccgcgtat ccgcacccgc cgacgccgtc gcacgtcccg
     5041 tgctcaccgt gaccaccgcg cccagcggtt tcgagggcga gggcttcccg gtgcgccgcg
     5101 cgttcgccgg gatcaactac cgccacctcg acccgttcat catgatggac cagatgggtg
     5161 aggtggagta cgcgcccggg gagcccaagg gcacgccctg gcacccgcac cgcggcttcg
     5221 agaccgtgac ctacatcgtc gacggtacct gaagccgaat tgacgtcagg tggcactttt
     5281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7021 gccttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc gtacctctca
     7081 tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt ggagccgggc
     7141 gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt catcctggag
     7201 acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc ccatgatgaa
     7261 ataacataag gtggtcccgt cgatagccga agcttaccga agtatacact taaattcagt
     7321 gcacgtttgc ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta
     7381 acccttacgt ggaataaaaa aaaatgaaat attgcaaatt ttgctgcaaa gctgtgactg
     7441 gagtaaaatt aattcacgtg ccgaagtgtg ctattaagag aaaattgtgg gagcagagcc
     7501 ttgggtgcag ccttggtgaa aactcccaaa tttgtgatac ccactttaat gattcgcagt
     7561 ggaaggctgc acctgcaaaa ggtcagacat ttaaaaggag gcgactcaac gcagatgccg
     7621 tacctagtaa agtgatagag cctgaaccag aaaagataaa agaaggctat accagtggga
     7681 gtacacaaac agagtaagtt tgaatagtaa aaaaaatcat ttatgtaaac aataacgtga
     7741 ctgtgcgtta ggtcctgttc attgtttaat gaaaataaga gcttgaggga aaaaattcgt
     7801 actttggagt acgaaatgcg tcgtttagag cagcagccga attaattcta gttccagtga
     7861 aatccaagca ttttctaaat taaatgtatt cttattatta tagttgttat ttttgatata
     7921 tataaacaac actattatgc ccaccatttt tttgagatgc atctacacaa ggaacaaaca
     7981 ctggatgtca ctttcagttc aaattgtaac gctaatcact ccgaacaggt cacaaaaaat
     8041 taccttaaaa agtcataata ttaaattaga ataaatatag ctgtgaggga aatatataca
     8101 aatatattgg agcaaataaa ttgtacatac aaatatttat tactaatttc tattgagacg
     8161 aaatgaacca ctcggaacca tttgagcgaa ccgaatcgcg cggaactaac gacagtcgct
     8221 ccaaggtcgt cgaacaaaag gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga
     8281 gcaactacga aacgtggtgt ggtggaggtg aattatgaag agggcgcgcg atttgaaaag
     8341 tatgtatata aaaaatatat cccggtgttt tatgtagcga taaacgagtt tttgatgtaa
     8401 ggtatgcagg tgtgtaagtc ttttggttag aagacaaatc caaagtctac ttgtggggat
     8461 gttcgaaggg gaaatacttg tattctatag gtcatatctt gtttttattg gcacaaatat
     8521 aattacatta gctttttgag ggggcaataa acagtaaaca cgatggtaat aatggtaaaa
     8581 aaaaaaaaca agcagttatt tcggatatat gtcggctact ccttgcgtcg ggcccgaagt
     8641 cttagagcca gatatgcgag cacccggaag ctcacgatga gaatggccag accatgatga
     8701 aataacataa ggtggtcccg tcggcaagag acatccactt aacgtatgct tgcaataagt
     8761 gcgagtgaaa ggaatagtat tctgagtgtc gtattgagtc tgagtgagac agcgatatga
     8821 ttgttgatta acccttagca tgtccgtggg gtttgaatta actcataata ttaattagac
     8881 gaaattattt ttaaagtttt atttttaata atttgcgagt acgcaaagct tggctgcatc
     8941 caacgcgttg ggagctctcc ggatccaagc ttgcatgcct gcaggtcgga gtactgtcct
     9001 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     9061 agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa atagaggcgc
     9121 ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg ctaagcgaaa
     9181 gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa agtgcaagtt
     9241 aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact actgaaatct
     9301 gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg ggaattcgtt
     9361 aacagatctg cggccgcatg gattacaagg atcacgacgg taaggattac aaggatgacg
     9421 acgataaggg cagcgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     9481 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     9541 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     9601 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     9661 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     9721 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     9781 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     9841 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     9901 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     9961 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
    10021 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
    10081 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
    10141 ctgctgctgc tgctgctggg
//
