LOCUS       sequence-338908-       15497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R 
            mutant signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15497)
  AUTHORS   Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar 
            N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, 
            Hecht JT
  TITLE     Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic 
            cleft lip and palate.
  JOURNAL   Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 
            10.1093/hmg/ddac037.
  PUBMED    35147171
REFERENCE   2  (bases 1 to 15497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2022-07-21- 21"; volume: "31"; issue: "14"; 
            pages: "2348-2357"
COMMENT     Sequence Label: pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter
FEATURES             Location/Qualifiers
     source          1..15497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3964..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4241..4263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4853..6642
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9301..9319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9387..9491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9492..9560,9561..10352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9710..9729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10523..11111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    11873..11921
                     /note="synthetic polyadenylation signal"
     misc_feature    11935..12026
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     11975..11994
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        12205..12236
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(12277..12279,12280..12282,12283..12993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12277..12279,12280..12282,12283..12993)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12322..12343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12583..12602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12930..12951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    13180..13301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13529..13674
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13717..14754
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(14752..14771)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14796..14844
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cgcctctggc cagctttcat taccctctat ccttccaaat gaaaatgaaa
      781 aggttgtgca gctgaattca tccttttctc tgagatgctt tggggagagt gaagtgagct
      841 ggcagtaccc catgtctgaa gaagagagct ccgatgtgga aatcagaaat gaagaaaaca
      901 acagcggcct ttttgtgacg gtcttggaag tgagcagtgc ctcggcggcc cacacagggt
      961 tgtacacttg ctattacaac cacactcaga cagaagagaa tgagcttgaa ggcaggcaca
     1021 tttacatcta tgtgccagac ccagatgtag cctttgtacc tctaggaatg acggattatt
     1081 tagtcatcgt ggaggatgat gattctgcca ttataccttg tcgcacaact gatcccgaga
     1141 ctcctgtaac cttacacaac agtgaggggg tggtacctgc ctcctatgac agcagacagg
     1201 gctttaatgg gaccttcact gtagggccct atatctgtga ggccaccgtc aaaggaaaga
     1261 agttccagac catcccattt aatgtttatg ctttaaaagc aacatcagag ctggatctag
     1321 aaatggaagc tcttaaaacc gtgtataagt caggggaaac gattgtggtc acctgtgctg
     1381 tttttaacaa tgaggtggtt gaccttcaat ggacttaccc tggagaagtg aaaggcaaag
     1441 gcatcacaat gctggaagaa atcaaagtcc catccatcaa attggtgtac actttgacgg
     1501 tccccgaggc cacggtgaaa gacagtggag attacgaatg tgctgcccgc caggctacca
     1561 gggaggtcaa agaaatgaag aaagtcacta tttctgtcca tgagaaaggt ttcattgaaa
     1621 tcaaacccac cttcagccag ttggaagctg tcaacctgca tgaagtcaaa cattttgttg
     1681 tagaggtgcg ggcctaccca cctcccagga tatcctggct gaaaaacaat ctgactctga
     1741 ttgaaaatct cactgagatc accactgatg tggaaaagat tcaggaaata aggtatcgaa
     1801 gcaaattaaa gctgatccgt gctaaggaag aagacagtgg ccattatact attgtagctc
     1861 aaaatgaaga tgctgtgaag agctatactt ttgaactgtt aactcaagtt ccttcatcca
     1921 ttctggactt ggtcgatgat caccatggct caactggggg acagaccgta cggtgcacag
     1981 ctgaaggcac gccgcttcct gatattgagt ggatgatatg caaagatatt aagaaatgta
     2041 ataatgaaac ttcctggact attttggcca acaatgtctc aaacatcatc acggagatcc
     2101 actcccgaga caggagtacc gtggagggcc gtgtgacttt cgccaaagtg gaggagacca
     2161 tcgccgtgcg atgcctggct aagaatctcc ttggagctga gaaccgagag ctgaagctgg
     2221 tggctcccac cctgcgttct gaactcacgg tggctgctgc agtcctggtg ctgttggtga
     2281 ttgtgatcat ctcacttatt gtcctggttg tcatttggaa acagaaaccg aggtatgaaa
     2341 ttcgctggag ggtcattgaa tcaatcagcc cggatggaca tgaatatatt tatgtggacc
     2401 cgatgcagct gccttatgac tcaagatggg agtttccaag agatggacta gtgcttggtc
     2461 gggtcttggg gtctggagcg tttgggaagg tggttgaagg aacagcctat ggattaagcc
     2521 ggtcccaacc tgtcatgaaa gttgcagtgc ggatgctaaa acccacggcc agatccagtg
     2581 aaaaacaagc tctcatgtct gaactgaaga taatgactca cctggggcca catttgaaca
     2641 ttgtaaactt gctgggagcc tgcaccaagt caggccccat ttacatcatc acagagtatt
     2701 gcttctatgg agatttggtc aactatttgc ataagaatag ggatagcttc ctgagccacc
     2761 acccagagaa gccaaagaaa gagctggata tctttggatt gaaccctgct gatgaaagca
     2821 cacggagcta tgttatttta tcttttgaaa acaatggtga ctacatggac atgaagcagg
     2881 ctgatactac acagtatgtc cccatgctag aaaggaaaga ggtttctaaa tattccgaca
     2941 tccagagatc actctatgat cgtccagcct catataagaa gaaatctatg ttagactcag
     3001 aagtcaaaaa cctcctttca gatgataact cagaaggcct tactttattg gatttgttga
     3061 gcttcaccta tcaagttgcc cgaggaatgg agtttttggc ttcaaaaaat tgtgtccacc
     3121 gtgatctggc tgctcgcaac gtcctcctgg cacaaggaaa aattgtgaag atctgtgact
     3181 ttggcctggc cagagacatc atgcatgatt cgaactatgt gtcgaaaggc agtacctttc
     3241 tgcccgtgaa gtggatggct cctgagagca tctttgacaa cctctacacc acactgagtg
     3301 atgtctggtc ttatggcatt ctgctctggg agatcttttc ccttggtggc accccttacc
     3361 ccggcatgat ggtggattct actttctaca ataagatcaa gagtgggtac cggatggcca
     3421 agcctgacca cgctaccagt gaagtctacg agatcatggt gaaatgctgg aacagtgagc
     3481 cggagaagag accctccttt taccacctga gtgagattgt ggagaatctg ctgcctggac
     3541 aatataaaaa gagttatgaa aaaattcacc tggacttcct gaagagtgac catcctgctg
     3601 tggcacgcat gcgtgtggac tcagacaatg catacattgg tgtcacctac aaaaacgagg
     3661 aagacaagct gaaggactgg gagggtggtc tggatgagca gagactgagc gctgacagtg
     3721 gctacatcat tcctctgcct gacattgacc ctgtccctga ggaggaggac ctgggcaaga
     3781 ggaacagaca cagctcgcag acctctgaag agagtgccat tgagacgggt tccagcagtt
     3841 ccaccttcat caagagagag gacgagacca ttgaagacat cgacatgatg gacgacatcg
     3901 gcatagactc ttcagacctg gtggaagaca gcttcctgta atgactcgag gccggcaagg
     3961 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4081 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4141 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     4201 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4261 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4321 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     4381 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcgggactac
     4441 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     4501 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     4561 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     4621 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     4681 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     4741 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     4801 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     4861 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     4921 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4981 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     5041 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     5101 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     5161 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     5221 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     5281 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     5341 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     5401 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     5461 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     5521 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     5581 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     5641 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     5701 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     5761 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     5821 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     5881 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     5941 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     6001 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     6061 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     6121 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     6181 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     6241 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     6301 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     6361 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     6421 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     6481 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgcatata
     6541 cagtcagcat atgataccca gtagtagagt gggagtgcta tcctttgcat atgccgccac
     6601 ctcccaaggg ggcgtgaatt ttcgctgctt gtccttttcc tgctggttgc tcccattctt
     6661 aggtgaattt aaggaggcca ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat
     6721 gtcgctaatg ttttccaacg cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca
     6781 tgggtatgcc caattgcccc atgttgggag gacgaaaatg gtgacaagac agatggccag
     6841 aaatacacca acagcacgca tgatgtctac tggggattta ttctttagtg cgggggaata
     6901 cacggctttt aatacgattg agggcgtctc ctaacaagtt acatcactcc tgcccttcct
     6961 caccctcatc tccatcacct ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag
     7021 ccccttccac cataggtgga aaccagggag gcaaatctac tccatcgtca aagctgcaca
     7081 cagtcaccct gatattgcag gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat
     7141 ccttcaaaac ctcagcaaat atatgagttt gtaaaaagac catgaaataa cagacaatgg
     7201 actcccttag cgggccaggt tgtgggccgg gtccaggggc cattccaaag gggagacgac
     7261 tcaatggtgt aagacgacat tgtggaatag caagggcagt tcctcgcctt aggttgtaaa
     7321 gggaggtctt actacctcca tatacgaaca caccggcgac ccaagttcct tcgtcggtag
     7381 tcctttctac gtgactccta gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt
     7441 tcgggttgga acctccttga ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc
     7501 ctccatcacc ctgaccccgg ggtccagtgc ttgggccttc tcctgggtca tctgcggggc
     7561 cctgctctat cgctcccggg ggcacgtcag gctcaccatc tgggccacct tcttggtggt
     7621 attcaaaata atcggcttcc cctacagggt ggaaaaatgg ccttctacct ggagggggcc
     7681 tgcgcggtgg agacccggat gatgatgact gactactggg actcctgggc ctcttttctc
     7741 cacgtccacg acctctcccc ctggctcttt cacgacttcc ccccctggct ctttcacgtc
     7801 ctctaccccg gcggcctcca ctacctcctc gaccccggcc tccactacct cctcgacccc
     7861 ggcctccact gcctcctcga ccccggcctc cacctcctgc tcctgcccct cctgctcctg
     7921 cccctcctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg
     7981 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctccccct cctcctcccc
     8041 ctcctccccc tcctccccct cctgcccctc ctcctcctcc tgctcctccc cctcctgcyc
     8101 ctcctcctcc tgcccctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg
     8161 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc ccctcctcct gctcctcccc
     8221 ctcctgctcc tgcccctcct gcccctcctg cccctcctgc tcctgcccct cctgctcctg
     8281 cccctcctgc ccctcctgcc cctcctgccc ctcctgcccc tcctccccct cccccccccc
     8341 cccccccccc ccctcccccc cccccccccc cccccccccc cccccccccc cccccccccc
     8401 cccccccccc cccccccccc cccccccccc ccccccctcc tccccctcct ccccctcccc
     8461 cccctcctcc tccccctccc cctcctcccc ctcctccccc tcctccccct cctccccctc
     8521 ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct cctgcccctc
     8581 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
     8641 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
     8701 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
     8761 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
     8821 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
     8881 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
     8941 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
     9001 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
     9061 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
     9121 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
     9181 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
     9241 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
     9301 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     9361 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9421 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     9481 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     9541 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     9601 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     9661 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     9721 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     9781 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     9841 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     9901 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     9961 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    10021 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    10081 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    10141 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    10201 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    10261 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    10321 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    10381 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    10441 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    10501 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    10561 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    10621 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    10681 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    10741 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    10801 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    10861 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    10921 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    10981 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    11041 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    11101 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    11161 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
    11221 tcccgagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg
    11281 aggtgccgcc ctgcttcatc cccgtggccc gttgcccgga gggagcttcc agggggaaac
    11341 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11401 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11461 ttcctggcct tttgctggcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
    11521 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
    11581 aaggccatcc agcctcgcgt cgcaaggatc ttgccgctat tgagatccag ttcgatatag
    11641 cccactcttg cacccagttg atcttcagca tcttttactt tcaccagcgt ttcggggtgt
    11701 gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg
    11761 atgctcatac tcgtcctttt tcaatattat tgaagcattt atcagggtta ctagtacgtc
    11821 tctcaaggat aagtaagtaa tattaaggta cgggaggtat tggacaggcc gcaataaaat
    11881 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
    11941 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
    12001 aagtgcaggt gccagaacat ttctctggcc taactggccg gtacctgagc tcactagcag
    12061 gatgtccata ttaggacatc taggatgtcc atattaggac atctaggatg tccatattag
    12121 gacatctagg atgtccatat taggacatct aggatgtcca tattaggaca tctagatctg
    12181 gcctcggcgg ccaagcttag acactagagg gtatataatg gaagctcgac ttccagcttg
    12241 gcaatccggt actgttggta aagccaccgg cgcgccatgg tgagcaaggg cgaggagctg
    12301 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
    12361 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
    12421 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
    12481 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
    12541 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
    12601 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
    12661 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
    12721 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
    12781 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
    12841 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
    12901 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
    12961 gggatcactc tcggcatgga cgagctgtac aagagtcatg gttttccccc ggaagtcgaa
    13021 gagcaggacg acgggacgct gccaatgtca tgcgcacaag agtctggtat ggatcgccac
    13081 ccggctgcgt gtgccagcgc gagaatcaat gtataatgag gcgcgcccta aggccgcgac
    13141 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
    13201 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
    13261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
    13321 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
    13381 acaaatgtgg taaaatcgat aagcgaacgc cagcaagacg tagcccagcg cttgcgaggt
    13441 gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga
    13501 aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt
    13561 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
    13621 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
    13681 caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga actcaccgcg
    13741 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
    13801 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
    13861 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
    13921 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
    13981 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
    14041 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
    14101 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
    14161 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
    14221 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
    14281 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
    14341 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
    14401 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
    14461 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
    14521 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
    14581 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
    14641 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
    14701 ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa ctgaaacacg
    14761 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
    14821 gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg
    14881 tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac
    14941 cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc
    15001 ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga atggtttatg
    15061 gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact ggactgagca
    15121 gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc gcgacacgaa
    15181 caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc gcggatttct
    15241 ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc agatccgggc
    15301 aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct atacattgaa
    15361 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
    15421 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
    15481 aatatgaccg ccatgtt
//
