LOCUS       sequence-338909-        8674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRISPR-associated transposases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Zhang Y, Yang J, Yang S, Zhang J, Chen J, Tao R, Jiang Y, Yang J, 
            Yang S
  TITLE     Programming Cells by Multicopy Chromosomal Integration Using 
            CRISPR-Associated Transposases.
  JOURNAL   CRISPR J. 2021 Jun;4(3):350-359. doi: 10.1089/crispr.2021.0018.
  PUBMED    34152213
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021"; journalName: "CRISPR J"; date: "2021-06"; 
            volume: "4"; issue: "3"; pages: "350-359"
COMMENT     Sequence Label: pAraQCas-BsaI
FEATURES             Location/Qualifiers
     source          1..8674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(67..85)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      81..128
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(299..1090)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1091..1182)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(2318..3196)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3223..3507
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     promoter        3778..3943
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 tatggcttat caaattcaga aaactcgttt cagcctgtac ccttaatctg acctaggctg
       61 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      121 gttttttgct gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca
      181 ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga
      241 cgaccgggtc atcgtggccg gatcttgcgg cccctcggct tgaacgaatt gttagacatt
      301 atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct tccaactgat
      361 ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta gcttcaagta
      421 tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg acatccttcg
      481 gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc actacatttc
      541 gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca tttagcgcct
      601 caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga cctaccaagg
      661 caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg atcgtggctg
      721 gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc agttcgcgct
      781 tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact tctacagcgc
      841 ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg atcaaagctc
      901 gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata tcactgtgtg
      961 gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac gtcggttcga
     1021 gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg gcgatcaccg
     1081 cttccctcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1141 tgagcggata catatttgaa tgtatttaga aaaataaaca aatagctagc tcactcggtc
     1201 gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag ttacccacag
     1261 attccgtgga taagcagggg actaacatgt gaggcaaaac agcagggccg cgccggtggc
     1321 gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct tttccggtgc
     1381 atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa cccgacagga
     1441 cttaaagatc cccaccgttt ccggcgggtc gctccctctt gcgctctcct gttccgaccc
     1501 tgccgtttac cggatacctg ttccgccttt ctcccttacg ggaagtgtgg cgctttctca
     1561 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     1621 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc
     1681 aacccggaaa agcacggtaa aacgccactg gcagcagcca ttggtaactg ggagttcgca
     1741 gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca
     1801 ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt
     1861 ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc gaaggttaag
     1921 tcagttgggg aactgcttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta
     1981 cagggcaaaa gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2041 ctgaaccgct ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc
     2101 ccatacgata taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct
     2161 gactgggttg aaggctctca agggcatcag tatattttag atgaagatta tttcttaatc
     2221 tagacatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2281 gcacatttcc ccgaaaagtg ccacctgcat cgatttatta tgacaacttg acggctacat
     2341 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     2401 taaatacccg cgagaagtag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     2461 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     2521 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     2581 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     2641 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     2701 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     2761 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     2821 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     2881 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     2941 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     3001 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     3061 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     3121 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     3181 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     3241 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     3301 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     3361 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     3421 cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     3481 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggaattcgag ctctaaagga
     3541 gatataccat gggtgaactg ccgagtaggt agctgataac gagacctctg gtctcgtgaa
     3601 ctgccgagta ggtagctgat aacggatccg aattcgagct cggcgcgcct gcaggtcgac
     3661 aagcttgcgg ccgcatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
     3721 tcgctaacca aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
     3781 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
     3841 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
     3901 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttgggaa
     3961 ttcgagctct aaaggagata tacatatgtt tttgcaaaga cctaaacctt acagcgatga
     4021 aagtttagaa agtttcttta tccgagtggc taacaaaaat ggctacggtg atgtccatcg
     4081 cttcctagaa gccactaaac gattccttca agacattgac cataatggct atcaaacctt
     4141 tccgactgat ataactcgga taaacccata ctcagctaaa aacagttcca gcgcacgaac
     4201 tgcgtcattc ctgaaacttg cacaattgac atttaatgaa ccgccagagc tacttgggtt
     4261 ggcaattaac agaacaaaca tgaaatactc gccgtcaact agcgcggttg ttagaggtgc
     4321 agaagtcttt cctcgcagtt tactacggac gcactccatc ccctgctgtc ctttgtgtct
     4381 gcgagaaaat ggctacgcct cctacctttg gcactttcag gggtacgaat actgccacag
     4441 ccataacgta cctttaatta ccacttgtag ctgtggtaag gagtttgact accgagtatc
     4501 tgggttaaag ggcatttgct gcaaatgcaa ggagcctatc accttaacca gcagggagaa
     4561 cggtcatgag gcagcgtgta ctgtttcaaa ctggcttgct ggccatgaat ctaaacctct
     4621 gccaaatctt cctaaaagct accgatgggg tttagttcat tggtggatgg gtattaaaga
     4681 tagcgagttc gatcactttt cgttcgttca atttttctca aactggccaa ggtcattcca
     4741 ctcgataatc gaagatgaag tagagttcaa ccttgagcat gctgttgtca gcacgtctga
     4801 attacgacta aaagatcttc ttggtcgatt gtttttcggt tcaattcggt tacctgagcg
     4861 gaatcttcaa cacaatatca tccttggtga gcttctctgc tatttagaaa atcgtttatg
     4921 gcaagacaag ggattaatcg ccaacctcaa aatgaacgcg ttagaggcga ctgtaatgtt
     4981 aaattgtagc ctcgatcaga ttgcatcaat ggttgaacaa cgcatcttga agccaaatcg
     5041 aaaaagcaag cccaacagcc ctcttgatgt taccgattat ctatttcatt tcggcgatat
     5101 tttctgtctt tggttagctg agttccaaag cgatgagttt aaccgttcgt tttatgtgtc
     5161 gaggtggtaa atgcaaactc tgaaagaact aatcgcatcc aatcctgacg acttaacaac
     5221 tgaacttaag agagcatttc gtccactcac accgcatatt gcaattgatg gtaatgaact
     5281 tgacgcactg acgatattag tcaatttaac cgataagact gatgatcaga aagacctgct
     5341 cgatcgagcc aaatgcaagc aaaaacttcg agatgaaaaa tggtgggcta gctgcataaa
     5401 ttgcgttaat tacagacaaa gccataaccc aaaattcccg gatatacgtt ctgaaggcgt
     5461 gattcgaacc caagccctgg gtgaattacc gagtttccta ctctcgtcct caaaaatccc
     5521 accataccat tggtcatata gccatgattc aaaatacgtc aacaaaagcg cattcctcac
     5581 caatgagttt tgttgggatg gtgagatctc atgtttgggt gagcttctta aagatgcaga
     5641 tcacccactt tggaatactt tgaaaaagtt aggttgttct caaaaaactt gcaaagcaat
     5701 ggcaaaacaa ctagctgata ttactctcac gactatcaat gtcacactcg caccaaatta
     5761 cctgactcaa atatctctcc ccgatagtga tacatcctac atttcactct ctcctgttgc
     5821 atcgctatcg atgcaaagcc actttcatca gaggctccaa gatgaaaatc ggcatagcgc
     5881 gataacgcgg tttagccgaa ctaccaacat gggggtcact gcgatgacat gtggcggcgc
     5941 atttagaatg ttaaagtctg gcgctaagtt ttccagcccc cctcatcacc gattaaacag
     6001 taaacgaagt tggttgacgt cagagcatgt tcagtcatta aaacagtacc agcgcctcaa
     6061 caaaagcctc atacctgaaa actctcggat tgcactccgt agaaaataca aaatcgagct
     6121 tcaaaatatg gtcagatctt ggtttgcaat gcaagaccat acccttgatt cgaacatact
     6181 tatccaacat ttgaatcatg acctatctta cttaggagcc acaaaacgtt ttgcatacga
     6241 tccagcgatg accaagctct ttactgagct tttgaaacga gagttatcaa attcaatcaa
     6301 taatggtgag caacacacta atggatcgtt tttagtccta ccgaatatca gggtttgtgg
     6361 cgcaacagct ttaagctccc cggtaacggt ggggattcca tcacttacag ctttctttgg
     6421 cttcgttcac gcatttgaac ggaatataaa tcgcaccacc tcatcgtttc gtgttgaatc
     6481 ctttgcgata tgcgtccatc aactacatgt cgagaagcga ggtttgacag cagagtttgt
     6541 ggaaaaaggc gacgggacta tatccgctcc cgcgacccgg gatgactggc agtgtgatgt
     6601 cgtatttagc cttattttga acaccaactt tgctcaacat attgaccaag atacgttagt
     6661 tacatcacta ccaaagcgat tggctcgggg ttcagcaaaa attgcgattg atgactttaa
     6721 acatatcaac tcattctcga cattagaaac agcgatcgaa tctctgccaa tagaagctgg
     6781 taggtggtta tcactttacg cacagtcaaa caataatcta agtgatctat tagcagccat
     6841 gacagaggac catcagctca tggcaagctg cgtcggttac cacttgttag aagagcccaa
     6901 agataaacca aactccctca gaggttacaa acacgctatc gccgagtgca tcattggact
     6961 cattaactca atcaccttta gctcagagac tgatcccaac acaatctttt ggtcgctaaa
     7021 gaactatcaa aactacctag tggtacagcc aaggagtatc aacgatgaaa ctaccgacaa
     7081 atctagccta tgagcgctct atcgacccat cagatgtctg tttttttgtc gtctggcccg
     7141 atgatagaaa aacaccttta acctacaatt ctcgtactct gctcgggcaa atggaagcgg
     7201 catcattagc ctatgatgtc tcaggtcaac caataaaaag tgccaccgct gaggcgttag
     7261 ctcaagggaa ccctcatcaa gttgatttct gccacgttcc atacggtgcg agtcatattg
     7321 aatgcagttt ctccgtctcg ttttcttctg aactacgtca accatataag tgtaactcaa
     7381 gcaaagttaa acaaacgcta gtgcaattag tcgagctcta cgaaacgaaa atcggctgga
     7441 ctgagctagc aacccgatat ttgatgaaca tttgcaacgg taaatggctg tggaaaaata
     7501 cccgtaaagc ctattgctgg aacattgtac ttacaccttg gccttggaac ggggaaaagg
     7561 ttggatttga agatattcgt actaactaca cctcacggca agactttaaa aataataaaa
     7621 attggtctgc tatagttgaa atgatcaaaa ccgcattttc tagtactgat gggctggcga
     7681 tatttgaagt cagggccacc ttgcacttgc caacgaatgc tatggtgcgg ccaagccaag
     7741 ttttcacaga aaaagaaagt ggcagtaaaa gtaaatctaa aactcaaaac agtcgagttt
     7801 ttcagagtac aactattgat ggtgaacgat cgccaatact aggggccttt aaaacgggag
     7861 cagctattgc aaccattgac gactggtatc ctgaagccac tgagccacta agggtcggac
     7921 ggtttggggt tcatcgcgaa gatgtcactt gctaccgtca tccgtctacc ggaaaagatt
     7981 ttttctcgat attacaacaa gcagagcact atattgaagt gttgagcgcc aacaaaactc
     8041 ccgctcaaga aactatcaac gacatgcact ttttaatggc taacctgatt aagggtggga
     8101 tgttccagca taaaggagac tgactgtgaa atggtattat aagacaatca cctttctgcc
     8161 agagttgtgc aacaacgagt cactggctgc aaagtgtctc cgcgttctgc atggatttaa
     8221 ctatcagtat gagacacgaa atatcggcgt ttcatttccg ctttggtgtg atgcaacggt
     8281 tggaaaaaag atttcatttg tcagcaagaa caagatagaa ctcgacttac tacttaaaca
     8341 acactatttc gtccaaatgg aacaacttca atactttcat atatccaaca ctgttctcgt
     8401 cccagaagat tgtacatacg tttcctttag acgctgtcaa tctatagata agctcacagc
     8461 agcagggctg gcaaggaaaa tcagacgcct ggagaaacgt gctctatcta gaggcgagca
     8521 atttgaccca tcatcttttg ctcaaaaaga gcatactgca atagcgcact accactcact
     8581 tggggagtcc agcaaacaga cgaaccgcaa ctttcgactc aatatcagga tgctctcgga
     8641 gcaaccccgt gaggggaact cgatttttag tagc
//
