LOCUS       sequence-338911-       15315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V 
            mutant signaling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Yu Y, Alvarado R, Petty LE, Bohlender RJ, Shaw DM, Below JE, Bejar 
            N, Ruiz OE, Tandon B, Eisenhoffer GT, Kiss DL, Huff CD, Letra A, 
            Hecht JT
  TITLE     Polygenic risk impacts PDGFRA mutation penetrance in non-syndromic 
            cleft lip and palate.
  JOURNAL   Hum Mol Genet. 2022 Jul 21;31(14):2348-2357. doi: 
            10.1093/hmg/ddac037.
  PUBMED    35147171
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2022-07-21- 21"; volume: "31"; issue: "14"; 
            pages: "2348-2357"
COMMENT     Sequence Label: pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter
FEATURES             Location/Qualifiers
     source          1..15315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             687..716
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    3964..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4241..4263)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4852..6641
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9119..9137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9205..9309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9310..9378,9379..10170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9528..9547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10341..10929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    11691..11739
                     /note="synthetic polyadenylation signal"
     misc_feature    11753..11844
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     11793..11812
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        12023..12054
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             join(12095..12097,12098..12100,12101..12811)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(12095..12097,12098..12100,12101..12811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(12140..12161)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12401..12420)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12748..12769
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    12998..13119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13347..13492
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13535..14572
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(14570..14589)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    14614..14662
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc atcgccctga
      661 gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag gacctgggca
      721 gccctggagg cgcctctggc cagctttcat taccctctat ccttccaaat gaaaatgaaa
      781 aggttgtgca gctgaattca tccttttctc tgagatgctt tggggagagt gaagtgagct
      841 ggcagtaccc catgtctgaa gaagagagct ccgatgtgga aatcagaaat gaagaaaaca
      901 acagcggcct ttttgtgacg gtcttggaag tgagcagtgc ctcggcggcc cacacagggt
      961 tgtacacttg ctattacaac cacactcaga cagaagagaa tgagcttgaa ggcaggcaca
     1021 tttacatcta tgtgccagac ccagatgtag cctttgtacc tctaggaatg acggattatt
     1081 tagtcatcgt ggaggatgat gattctgcca ttataccttg tcgcacaact gatcccgaga
     1141 ctcctgtaac cttacacaac agtgaggggg tggtacctgc ctcctatgac agcagacagg
     1201 gctttaatgg gaccttcact gtagggccct atatctgtga ggccaccgtc aaaggaaaga
     1261 agttccagac catcccattt aatgtttatg ctttaaaagc aacatcagag ctggatctag
     1321 aaatggaagc tcttaaaacc gtgtataagt caggggaaac gattgtggtc acctgtgctg
     1381 tttttaacaa tgaggtggtt gaccttcaat ggacttaccc tggagaagtg aaaggcaaag
     1441 gcatcacaat gctggaagaa atcaaagtcc catccatcaa attggtgtac actttgacgg
     1501 tccccgaggc cacggtgaaa gacagtggag attacgaatg tgctgcccgc caggctacca
     1561 gggaggtcaa agaaatgaag aaagtcacta tttctgtcca tgagaaaggt ttcattgaaa
     1621 tcaaacccac cttcagccag ttggaagctg tcaacctgca tgaagtcaaa cattttgttg
     1681 tagaggtgcg ggcctaccca cctcccagga tatcctggct gaaaaacaat ctgactctga
     1741 ttgaaaatct cactgagatc accactgatg tggaaaagat tcaggaaata aggtatcgaa
     1801 gcaaattaaa gctgatccgt gctaaggaag aagacagtgg ccattatact attgtagctc
     1861 aaaatgaaga tgctgtgaag agctatactt ttgaactgtt aactcaagtt ccttcatcca
     1921 ttctggactt ggtcgatgat caccatggct caactggggg acagaccgta cggtgcacag
     1981 ctgaaggcac gccgcttcct gatattgagt ggatgatatg caaagatatt aagaaatgta
     2041 ataatgaaac ttcctggact attttggcca acaatgtctc aaacatcatc acggagatcc
     2101 actcccgaga caggagtacc gtggagggcc gtgtgacttt cgccaaagtg gaggagacca
     2161 tcgccgtgcg atgcctggct aagaatctcc ttggagctga gaaccgagag ctgaagctgg
     2221 tggctcccac cctgcgttct gaactcacgg tggctgctgc agtcctggtg ctgttggtga
     2281 ttgtgatcat ctcacttatt gtcctggttg tcatttggaa acagaaaccg aggtatgaaa
     2341 ttcgctggag ggtcattgaa tcaatcagcc cggatggaca tgaatatatt tatgtggacc
     2401 cgatgcagct gccttatgac tcaagatggg agtttccaag agatggacta gtgcttggtc
     2461 gggtcttggg gtctggagcg tttgggaagg tggttgaagg aacagcctat ggattaagcc
     2521 ggtcccaacc tgtcatgaaa gttgcagtga agatgctaaa acccacggcc agatccagtg
     2581 aaaaacaagc tctcatgtct gaactgaaga taatgactca cctggggcca catttgaaca
     2641 ttgtaaactt gctgggagcc tgcaccaagt caggccccat ttacatcatc acagagtatt
     2701 gcttctatgg agatttggtc aactatttgc ataagaatag ggatagcttc ctgagccacc
     2761 acccagagaa gccaaagaaa gagctggata tctttggatt gaaccctgct gatgaaagca
     2821 cacggagcta tgttatttta tcttttgaaa acaatggtga ctacatggac atgaagcagg
     2881 ctgatactac acagtatgtc cccatgctag aaaggaaaga ggtttctaaa tattccgaca
     2941 tccagagatc actctatgat cgtccagcct catataagaa gaaatctatg ttagactcag
     3001 aagtcaaaaa cctcctttca gatgataact cagaaggcct tactttattg gatttgttga
     3061 gcttcaccta tcaagttgcc cgaggaatgg agtttttggc ttcaaaaaat tgtgtccacc
     3121 gtgatctggc tgctcgcaac gtcctcctgg cacaaggaaa aattgtgaag atctgtgact
     3181 ttggcctggc cagagtcatc atgcatgatt cgaactatgt gtcgaaaggc agtacctttc
     3241 tgcccgtgaa gtggatggct cctgagagca tctttgacaa cctctacacc acactgagtg
     3301 atgtctggtc ttatggcatt ctgctctggg agatcttttc ccttggtggc accccttacc
     3361 ccggcatgat ggtggattct actttctaca ataagatcaa gagtgggtac cggatggcca
     3421 agcctgacca cgctaccagt gaagtctacg agatcatggt gaaatgctgg aacagtgagc
     3481 cggagaagag accctccttt taccacctga gtgagattgt ggagaatctg ctgcctggac
     3541 aatataaaaa gagttatgaa aaaattcacc tggacttcct gaagagtgac catcctgctg
     3601 tggcacgcat gcgtgtggac tcagacaatg catacattgg tgtcacctac aaaaacgagg
     3661 aagacaagct gaaggactgg gagggtggtc tggatgagca gagactgagc gctgacagtg
     3721 gctacatcat tcctctgcct gacattgacc ctgtccctga ggaggaggac ctgggcaaga
     3781 ggaacagaca cagctcgcag acctctgaag agagtgccat tgagacgggt tccagcagtt
     3841 ccaccttcat caagagagag gacgagacca ttgaagacat cgacatgatg gacgacatcg
     3901 gcatagactc ttcagacctg gtggaagaca gcttcctgta atgactcgag gccggcaagg
     3961 ccggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4081 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4141 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     4201 gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     4261 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     4321 cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta gaggatcgat
     4381 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     4441 gcatctctgc cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac
     4501 ttgccaactg ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct
     4561 ctgactgtag ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt
     4621 catcctggag cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac
     4681 tcttggctga agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag
     4741 actacgggag gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac
     4801 cctcaagagg gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag
     4861 catatgcttc ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg
     4921 ttacccaacg ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag
     4981 cgatatctcc caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga
     5041 gggtagtgaa ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa
     5101 ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct
     5161 gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc
     5221 cagaataaaa tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc
     5281 ctcacaaacc ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta
     5341 acaatagaaa tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa
     5401 tttatggcta tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt
     5461 gtcccaggca gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg
     5521 aaagagagtg gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat
     5581 taaacggggc tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt
     5641 gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt
     5701 aaaataaggg tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac
     5761 ttgcccacaa aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc
     5821 aagatagggg cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg
     5881 ccaagcacag ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat
     5941 gttgccatgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     6001 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     6061 atctgggtag tatatgctat cctaatttat atctgggtag cataggctat cctaatctat
     6121 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt
     6181 atccgggtag catatgctat cctaatagag attagggtag tatatgctat cctaatttat
     6241 atctgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     6301 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
     6361 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atttatatct
     6421 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     6481 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac
     6541 agtcagcata tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc
     6601 tcccaagggg gcgtgaattt tcgctgcttg tccttttcct gctggttgct cccattctta
     6661 ggtgaattta aggaggccag gctaaagccg tcgcatgtct gattgctcac caggtaaatg
     6721 tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat
     6781 gggtatgccc aattgcccca tgttgggagg acgaaaatgg tgacaagaca gatggccaga
     6841 aatacaccaa cagcacgcat gatgtctact ggggatttat tctttagtgc gggggaatac
     6901 acggctttta atacgattga gggcgtctcc taacaagtta catcactcct gcccttcctc
     6961 accctcatct ccatcacctc cttcatctcc gtcatctccg tcatcaccct ccgcggcagc
     7021 cccttccacc ataggtggaa accagggagg caaatctact ccatcgtcaa agctgcacac
     7081 agtcaccctg atattgcagg taggagcggg ctttgtcata acaaggtcct taatcgcatc
     7141 cttcaaaacc tcagcaaata tatgagtttg taaaaagacc atgaaataac agacaatgga
     7201 ctcccttagc gggccaggtt gtgggccggg tccaggggcc attccaaagg ggagacgact
     7261 caatggtgta agacgacatt gtggaatagc aagggcagtt cctcgcctta ggttgtaaag
     7321 ggaggtctta ctacctccat atacgaacac accggcgacc caagttcctt cgtcggtagt
     7381 cctttctacg tgactcctag ccaggagagc tcttaaacct tctgcaatgt tctcaaattt
     7441 cgggttggaa cctccttgac cacgatgctt tccaaaccac cctccttttt tgcgcctgcc
     7501 tccatcaccc tgaccccggg gtccagtgct tgggccttct cctgggtcat ctgcggggcc
     7561 ctgctctatc gctcccgggg gcacgtcagg ctcaccatct gggccacctt cttggtggta
     7621 ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc cttctacctg gagggggcct
     7681 gcgcggtgga gacccggatg atgatgactg actactggga ctcctgggcc tcttttctcc
     7741 acgtccacga cctctccccc tggctctttc acgacttccc cccctggctc tttcacgtcc
     7801 tctaccccgg cggcctccac tacctcctcg accccggcct ccactacctc ctcgaccccg
     7861 gcctccactg cctcctcgac cccggcctcc acctcctgct cctgcccctc ctgctcctgc
     7921 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     7981 tcctgctcct gttcccccgt gggtcccttt gccgcccatg ccacttggcc gtttttgggg
     8041 tcccgcsccc cctccttytc ctkcycctgh tgcccctcct bcccgysctc cccctcttcc
     8101 ccccccacac cccctgctca cccccccccc cmmcmacscg cgccsvcycc mcaccccccc
     8161 cctccccccc cccccccccc cccccccccc ccccctcccc cccccccccc cccgccccct
     8221 cccccccccc cccccccccc cccccccccc cccccccgcc cccggcctcc actacctcct
     8281 cgaccccggc ctccactgcc tcctcgaccc cggcctccac ctcctgctcc tgcccctcct
     8341 gctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     8401 cctcctgctc ctgctcctgt tccaccgtgg gtccctttgc agccaatgca acttggacgt
     8461 ttttggggtc tccggacacc atctctatgt cttggccctg atcctgagcc gcccggggct
     8521 cctggtcttc cgcctcctcg tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc
     8581 cctcttcttt gaggtccact gccgccggag ccttctggtc cagatgtgtc tcccttctct
     8641 cctaggccat ttccaggtcc tgtacctggc ccctcgtcag acatgattca cactaaaaga
     8701 gatcaataga catctttatt agacgacgct cagtgaatac agggagtgca gactcctgcc
     8761 ccctccaaca gcccccccac cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc
     8821 tgaaaattcc ccatcctccg aaccatcctc gtcctcatca ccaattactc gcagcccgga
     8881 aaactcccgc tgaacatcct caagatttgc gtcctgagcc tcaagccagg cctcaaattc
     8941 ctcgtccccc tttttgctgg acggtaggga tggggattct cgggacccct cctcttcctc
     9001 ttcaaggtca ccagacagag atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg
     9061 tgaggatcag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc
     9121 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     9181 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     9241 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     9301 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9361 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9421 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9481 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9541 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9601 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9661 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9721 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9781 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9841 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9901 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9961 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    10021 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    10081 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    10141 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    10201 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    10261 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    10321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggccttga
    10981 agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc
    11041 ccgagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag
    11101 gtgccgccct gcttcatccc cgtggcccgt tgctcgcgtt tgccttccag ggggaaacgc
    11161 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    11221 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    11281 cctggccttt tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag
    11341 catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa
    11401 ggccatccag cctcgcgtcg caaggatctt gccgctattg agatccagtt cgatatagcc
    11461 cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc
    11521 aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat
    11581 gctcatactc gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc
    11641 tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat
    11701 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
    11761 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
    11821 gtgcaggtgc cagaacattt ctctggccta actggccggt acctgagctc actagcagga
    11881 tgtccatatt aggacatcta ggatgtccat attaggacat ctaggatgtc catattagga
    11941 catctaggat gtccatatta ggacatctag gatgtccata ttaggacatc tagatctggc
    12001 ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt ccagcttggc
    12061 aatccggtac tgttggtaaa gccaccggcg cgccatggtg agcaagggcg aggagctgtt
    12121 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
    12181 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
    12241 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
    12301 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
    12361 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
    12421 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
    12481 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
    12541 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
    12601 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
    12661 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
    12721 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
    12781 gatcactctc ggcatggacg agctgtacaa gagtcatggt tttcccccgg aagtcgaaga
    12841 gcaggacgac gggacgctgc caatgtcatg cgcacaagag tctggtatgg atcgccaccc
    12901 ggctgcgtgt gccagcgcga gaatcaatgt ataatgaggc gcgccctaag gccgcgactc
    12961 tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga
    13021 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
    13081 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
    13141 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
    13201 aaatgtggta aaatcgataa gcgaacgcca gcaagacgta gcccagcgtt agcgaggtgc
    13261 cgccctgctt catccccgtg gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa
    13321 tatatttgca tgtctttagt tctatgatga cacaaacccc gcccagcgtc ttgtcattgg
    13381 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
    13441 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
    13501 acagcgtgcc gcagatcccg gggggcaatg agatatgaaa aagcctgaac tcaccgcgac
    13561 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
    13621 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
    13681 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
    13741 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
    13801 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
    13861 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
    13921 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
    13981 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
    14041 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
    14101 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
    14161 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
    14221 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
    14281 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
    14341 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
    14401 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
    14461 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
    14521 aaaccgacgc cccagcactc gtccggatcg ggagatgggg gaggctaact gaaacacgga
    14581 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
    14641 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
    14701 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
    14761 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
    14821 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
    14881 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgactgg actgagcaga
    14941 cagacccatg gtttttggat ggcctgggca tggaccgcat gtactggcgc gacacgaaca
    15001 ccgggcgtct gtggctgcca aacacccccg acccccaaaa accaccgcgc ggatttctgg
    15061 cgtgccaagc tagtcgacca attctcatgt ttgacagctt atcatcgcag atccgggcaa
    15121 cgttgttgcc attgctgcag gcgcagaact ggtaggtatg gaagatctat acattgaatc
    15181 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
    15241 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
    15301 tatgaccgcc atgtt
//
