LOCUS       sequence-338925-       10733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of envA-expressing stable cell lines, resistant to the 
            antibiotic Neomycin (Gentamicin/G418).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: pLV-EF1a-N2c_envA-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..10733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5331..5880
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5475..5492)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5702..5721
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5883..6677
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5937..5956)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6547..6566
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6701..7289
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6754..6774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7172..7183)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7496..8129
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8257..8273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8257..8273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8270..8292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8281..8297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8305..8311,8312..8329,8330..8335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8350..8371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8488..8505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8659..9247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8739..8758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9418..10209,10210..10278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10041..10060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10279..10383)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10431..10565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10468..10487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10522..10541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10733,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccgcggc cgccatggaa gccgtcataa aggcatttct
     3421 gactgggcac cctggaaagg tgagcaagaa ggactccaag aagaagccgc cagcaacaag
     3481 caagaaagac ccggagaaga cgcccttgct gccaacgaga gttaattata ttattggtgt
     3541 cctggtcttg tgtgaggtaa cgggggtaag agctgatgtc cacttactcg agcagccagg
     3601 gaacctttgg attacatggg ccaaccgtac aggccaaacg gatttttgcc tctctacaca
     3661 gtcagccacc tccccttttc aaacatgttt gataggtatc ccgtccccta tttccgaggg
     3721 tgattttaag ggatatgttt ctgatacaaa ttgcaccacc ttgggaactg atcggttagt
     3781 ctcgtcagcc gactttactg gcggacctga caacagtacc accctcactt atcggaaggt
     3841 ctcatgcttg ttgttaaagc tgaatgtctc tatgtgggat gagccacctg aactacagct
     3901 gttaggttcc cagtctctcc ctaacattac taatattgct cagatttccg gtataaccgg
     3961 gggatgcgta ggcttcagac cacaaggggt tccttggtat ctaggttggt ctagacagga
     4021 ggccacgcgg tttctcctta gacacccctc tttctctaaa tccacggaac cgtttacggt
     4081 ggtgacagcg gataggcaca atctttttat ggggagtgag tactgcggtg catatggcta
     4141 cagattttgg aacatgtata actgctcaca ggtggggcgg cagtaccgct gtggtaatgc
     4201 gcgcagcccc cgcccgggtc ttcctgaaat ccagtgtaca aggagaggag gcaaatgggt
     4261 taatcaatca caggaaatta atgagtcgga gccgttcagc tttacggtga actgtacagc
     4321 tagtagtttg ggtaatgcca gtgggtgttg cggaaaagca ggcacgattc tcccgggaaa
     4381 gtgggtcgac agcacacaag gtagtttcac caaaccaaaa gcgctaccac ccgcaatttt
     4441 cctcatttgt ggggatcgcg catggcaagg aattcccagt cgtccggtag ggggcccctg
     4501 ctatttaggc aagcttacca tgttagcacc taagcataca gatattctca aggtgcttgt
     4561 caattcatcg cggacaggta taagacgtaa acgaagcacc tcacacctgg atgatacatg
     4621 ctcagatgaa gtgcagcttt ggggtcctac agcaagaatc tttgcatcta tcctagcccc
     4681 gggggtagca gctgcgcaag ccttaagaga aattgagaga ctagcctgtt ggtccgttaa
     4741 acaggctaac ttgacaacat cactcctcgg ggacttattg gatgatgtca cgagtattcg
     4801 acacgcggtc ctgcagaacc gagcggctat tgacttcttg ctcctagctc acggccatgg
     4861 ctgtgaggac gttgccggaa tgtgctgttt caatttgagt gatcacagtg agtctataca
     4921 gaagaagttc cagctaatga aggaacatgt caataagatc ggcgtggaca gcgacccaat
     4981 tggaagttgg ctgcgaggac tattcggggg aataggagaa tgggccgttc atttgctgaa
     5041 aggactgctt ttggggcttg tagttatttt gttgctagta gtgtgcctgc cttgcagaag
     5101 agccaatcga ccagaatcga aacaacgcag ttttggaggg acagggggga atgtgtcagt
     5161 cacttcccaa agcggaaaag tcataccttc atgggaatca tataagagtg gaggtgagat
     5221 cagactgtaa gcggccgctc tagagtcgac gggccgcggt aacaattgtt aactaactta
     5281 agctagcaac ggtttccctc tagcgggatc aattccgccc ccccccccta acgttactgg
     5341 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     5401 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5461 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     5521 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     5581 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     5641 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     5701 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     5761 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     5821 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgataata
     5881 ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     5941 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     6001 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     6061 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     6121 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     6181 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     6241 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     6301 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     6361 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     6421 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     6481 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     6541 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     6601 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     6661 ttgacgagtt cttctgaccc gggcggggcg cgtctggaac aatcaacctc tggattacaa
     6721 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6781 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6841 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6901 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6961 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7021 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7081 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7141 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7201 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7261 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
     7321 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     7381 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     7441 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     7501 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     7561 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     7621 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     7681 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     7741 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     7801 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     7861 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     7921 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     7981 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8041 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8101 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8161 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     8221 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8281 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8341 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8401 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8461 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9061 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9541 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10381 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta
    10441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
    10501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
    10561 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
    10621 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
    10681 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtt
//
