LOCUS       sequence-339004-       17820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted 
            mutagenesis with a mCherry as fluorescent marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17820)
  AUTHORS   McCaw ME, Lee K, Kang M, Zobrist JD, Azanu MK, Birchler JA, Wang K
  TITLE     Development of a Transformable Fast-Flowering Mini-Maize as a Tool 
            for Maize Gene Editing.
  JOURNAL   Front Genome Ed. 2021 Jan 11;2:622227. doi: 
            10.3389/fgeed.2020.622227. eCollection 2020.
  PUBMED    34713243
REFERENCE   2  (bases 1 to 17820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Genome Ed."; date: "2021-01-11"; volume: "2:622227. doi"; pages: " 
            10.3389/fgeed.2020.622227. eCollection 2020"
COMMENT     Sequence Label: pKL2013
FEATURES             Location/Qualifiers
     source          1..17820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     174..196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     188..205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     189..205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        221..2218
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    2261..2285
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2307..2372
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2379..2399
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2424..6524
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             2424..6524
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6437..6445
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6525..6572
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    7247..7267
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7304..7681
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        7702..7777
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(7866..7890)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8426..9130)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELY"
     primer_bind     complement(8698..8717)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(9200..9545)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9666..9682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9666..9682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9679..9701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9690..9706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9714..9720,9721..9738,9739..9744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9759..9780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9971..10647
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10692..11243
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11250..11424
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11502..11526
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11951..12745
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      12832..13420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13321..13340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13574..13591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13606..13746
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13672..13691)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13832..13854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14090..14284
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14350..15423)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15852..16481)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    17782..17806
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt
       61 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
      121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      181 acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca gcgtgacccg
      241 gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca
      301 tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa
      361 ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc
      421 atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc
      481 tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct
      541 atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt
      601 atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac
      661 taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag
      721 tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct
      781 tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac
      841 cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc
      901 tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat
      961 ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc
     1021 tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc
     1081 tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt
     1141 cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc
     1201 aaggtacgcc gctcgtcctc cccccccccc ccctctctac cttctctaga tcggcgttcc
     1261 ggtccatggt tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt
     1321 gtgttagatc cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc
     1381 tgattgctaa cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg
     1441 cagacgggat cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt
     1501 tcctttattt caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt
     1561 tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattaa
     1621 ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca
     1681 tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat
     1741 gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg
     1801 atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa
     1861 ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta
     1921 cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt
     1981 actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac
     2041 ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg
     2101 atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt
     2161 tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggt
     2221 cgactctaga ggatcccctc gaggcgcgcc aagctatcaa acaagtttgt acaaaaaagc
     2281 aggctccgaa ttcgcccttc accatggatt acaaggacca cgacggggat tacaaggacc
     2341 acgacattga ttacaaggat gatgatgaca agatggctcc gaagaagaag aggaaggttg
     2401 gcatccacgg ggtgccagct gctgacaaga agtactcgat cggcctcgat attgggacta
     2461 actctgttgg ctgggccgtg atcaccgacg agtacaaggt gccctcaaag aagttcaagg
     2521 tcctgggcaa caccgatcgg cattccatca agaagaatct cattggcgct ctcctgttcg
     2581 acagcggcga gacggctgag gctacgcggc tcaagcgcac cgcccgcagg cggtacacgc
     2641 gcaggaagaa tcgcatctgc tacctgcagg agattttctc caacgagatg gcgaaggttg
     2701 acgattcttt cttccacagg ctggaggagt cattcctcgt ggaggaggat aagaagcacg
     2761 agcggcatcc aatcttcggc aacattgtcg acgaggttgc ctaccacgag aagtacccta
     2821 cgatctacca tctgcggaag aagctcgtgg actccacaga taaggcggac ctccgcctga
     2881 tctacctcgc tctggcccac atgattaagt tcaggggcca tttcctgatc gagggggatc
     2941 tcaacccgga caatagcgat gttgacaagc tgttcatcca gctcgtgcag acgtacaacc
     3001 agctcttcga ggagaacccc attaatgcgt caggcgtcga cgcgaaggct atcctgtccg
     3061 ctaggctctc gaagtctcgg cgcctcgaga acctgatcgc ccagctgccg ggcgagaaga
     3121 agaacggcct gttcgggaat ctcattgcgc tcagcctggg gctcacgccc aacttcaagt
     3181 cgaatttcga tctcgctgag gacgccaagc tgcagctctc caaggacaca tacgacgatg
     3241 acctggataa cctcctggcc cagatcggcg atcagtacgc ggacctgttc ctcgctgcca
     3301 agaatctgtc ggacgccatc ctcctgtctg atattctcag ggtgaacacc gagattacga
     3361 aggctccgct ctcagcctcc atgatcaagc gctacgacga gcaccatcag gatctgaccc
     3421 tcctgaaggc gctggtcagg cagcagctcc ccgagaagta caaggagatc ttcttcgatc
     3481 agtcgaagaa cggctacgct gggtacattg acggcggggc ctctcaggag gagttctaca
     3541 agttcatcaa gccgattctg gagaagatgg acggcacgga ggagctgctg gtgaagctca
     3601 atcgcgagga cctcctgagg aagcagcgga cattcgataa cggcagcatc ccacaccaga
     3661 ttcatctcgg ggagctgcac gctatcctga ggaggcagga ggacttctac cctttcctca
     3721 aggataaccg cgagaagatc gagaagattc tgactttcag gatcccgtac tacgtcggcc
     3781 cactcgctag gggcaactcc cgcttcgctt ggatgacccg caagtcagag gagacgatca
     3841 cgccgtggaa cttcgaggag gtggtcgaca agggcgctag cgctcagtcg ttcatcgaga
     3901 ggatgacgaa tttcgacaag aacctgccaa atgagaaggt gctccctaag cactcgctcc
     3961 tgtacgagta cttcacagtc tacaacgagc tgactaaggt gaagtatgtg accgagggca
     4021 tgaggaagcc ggctttcctg tctggggagc agaagaaggc catcgtggac ctcctgttca
     4081 agaccaaccg gaaggtcacg gttaagcagc tcaaggagga ctacttcaag aagattgagt
     4141 gcttcgattc ggtcgagatc tctggcgttg aggaccgctt caacgcctcc ctggggacct
     4201 accacgatct cctgaagatc attaaggata aggacttcct ggacaacgag gagaatgagg
     4261 atatcctcga ggacattgtg ctgacactca ctctgttcga ggaccgggag atgatcgagg
     4321 agcgcctgaa gacttacgcc catctcttcg atgacaaggt catgaagcag ctcaagagga
     4381 ggaggtacac cggctggggg aggctgagca ggaagctcat caacggcatt cgggacaagc
     4441 agtccgggaa gacgatcctc gacttcctga agagcgatgg cttcgcgaac cgcaatttca
     4501 tgcagctgat tcacgatgac agcctcacat tcaaggagga tatccagaag gctcaggtga
     4561 gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcg ccagctatca
     4621 agaaggggat tctgcagacc gtgaaggttg tggacgagct ggtgaaggtc atgggcaggc
     4681 acaagcctga gaacatcgtc attgagatgg cccgggagaa tcagaccacg cagaagggcc
     4741 agaagaactc acgcgagagg atgaagagga tcgaggaggg cattaaggag ctggggtccc
     4801 agatcctcaa ggagcacccg gtggagaaca cgcagctgca gaatgagaag ctctacctgt
     4861 actacctcca gaatggccgc gatatgtatg tggaccagga gctggatatt aacaggctca
     4921 gcgattacga cgtcgatcat atcgttccac agtcattcct gaaggatgac tccattgaca
     4981 acaaggtcct caccaggtcg gacaagaacc ggggcaagtc tgataatgtt ccttcagagg
     5041 aggtcgttaa gaagatgaag aactactggc gccagctcct gaatgccaag ctgatcacgc
     5101 agcggaagtt cgataacctc acaaaggctg agaggggcgg gctctctgag ctggacaagg
     5161 cgggcttcat caagaggcag ctggtcgaga cacggcagat cactaagcac gttgcgcaga
     5221 ttctcgactc acggatgaac actaagtacg atgagaatga caagctgatc cgcgaggtga
     5281 aggtcatcac cctgaagtca aagctcgtct ccgacttcag gaaggatttc cagttctaca
     5341 aggttcggga gatcaacaat taccaccatg cccatgacgc gtacctgaac gcggtggtcg
     5401 gcacagctct gatcaagaag tacccaaagc tcgagagcga gttcgtgtac ggggactaca
     5461 aggtttacga tgtgaggaag atgatcgcca agtcggagca ggagattggc aaggctaccg
     5521 ccaagtactt cttctactct aacattatga atttcttcaa gacagagatc actctggcca
     5581 atggcgagat ccggaagcgc cccctcatcg agacgaacgg cgagacgggg gagatcgtgt
     5641 gggacaaggg cagggatttc gcgaccgtca ggaaggttct ctccatgcca caagtgaata
     5701 tcgtcaagaa gacagaggtc cagactggcg ggttctctaa ggagtcaatt ctgcctaagc
     5761 ggaacagcga caagctcatc gcccgcaaga aggactggga tccgaagaag tacggcgggt
     5821 tcgacagccc cactgtggcc tactcggtcc tggttgtggc gaaggttgag aagggcaagt
     5881 ccaagaagct caagagcgtg aaggagctgc tggggatcac gattatggag cgctccagct
     5941 tcgagaagaa cccgatcgat ttcctggagg cgaagggcta caaggaggtg aagaaggacc
     6001 tgatcattaa gctccccaag tactcactct tcgagctgga gaacggcagg aagcggatgc
     6061 tggcttccgc tggcgagctg cagaagggga acgagctggc tctgccgtcc aagtatgtga
     6121 acttcctcta cctggcctcc cactacgaga agctcaaggg cagccccgag gacaacgagc
     6181 agaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatt gagcagattt
     6241 ccgagttctc caagcgcgtg atcctggccg acgcgaatct ggataaggtc ctctccgcgt
     6301 acaacaagca ccgcgacaag ccaatcaggg agcaggctga gaatatcatt catctcttca
     6361 ccctgacgaa cctcggcgcc cctgctgctt tcaagtactt cgacacaact atcgatcgca
     6421 agaggtacac aagcactaag gaggtcctgg acgcgaccct catccaccag tcgattaccg
     6481 gcctctacga gacgcgcatc gacctgtctc agctcggggg cgacaagcgg ccagcggcga
     6541 cgaagaaggc ggggcaggcg aagaagaaga agtgagctca gagctttcgt tcgtatcatc
     6601 ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc
     6661 tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct
     6721 tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg
     6781 gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt
     6841 tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt
     6901 tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac
     6961 acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa
     7021 aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt
     7081 tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta taattatagt
     7141 tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc
     7201 aattgttccg gaactctaga taagcttacc ggaaagggcg aattcgcaac tttgtataca
     7261 aaagttgccc catggcgttc cctctagata acgcaggatc ctaaagggat ctttaaacat
     7321 acgaacagat cacttaaagt tcttctgaag caacttaaag ttatcaggca tgcatggatc
     7381 ttggaggaat cagatgtgca gtcagggacc atagcacagg acaggcgtct tctactggtg
     7441 ctaccagcaa atgctggaag ccgggaacac tgggtacgtt ggaaaccacg tgatgtggag
     7501 taagataaac tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt
     7561 attgcagtat gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc
     7621 acctcggcta tccacataga tcaaagctgg tttaaaagag ttgtgcagat gatccgtggc
     7681 acagatcaca aacttcaaat ggttttagag ctagaaatag caagttaaaa taaggctagt
     7741 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgtccct tcgaagggca
     7801 attctgcaga tatccatcac actggcggcc gctcgaggtc gagggtatcg ataagcttga
     7861 attcgaccca gctttcttgt acaaagtggt tcgataattc ttaatcgccg cttattgcac
     7921 tcccttttaa ctgtttttta ttacaaaaat gccctggaaa atgcactccc tttttgtgtt
     7981 tgtttttttg tgaaacgatg ttgtcaggta atttatttgt cagtctacta tggtggccca
     8041 ttatattaat agcaactgtc ggtccaatag acgacgtcga ttttctgcat ttgtttaacc
     8101 acgtggattt tatgacattt tatattagtt aatttgtaaa acctacccaa ttaaagacct
     8161 catatgttct aaagactaat acttaatgat aacaattttc ttttagtgaa gaaagggata
     8221 attagtaaat atggaacaag ggcagaagat ttattaaagc cgcgtaagag acaacaagta
     8281 ggtacgtgga gtgtcttagg tgacttaccc acataacata aagtgacatt aacaaacata
     8341 gctaatgctc ctatttgaat agtgcatatc agcatacctt attacatata gataggagca
     8401 aactctagct accgctttaa gatctgtaca gctcctccat gccgccggtg gagtggcggc
     8461 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     8521 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
     8581 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
     8641 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct
     8701 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
     8761 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     8821 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     8881 tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt
     8941 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct
     9001 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     9061 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
     9121 tgctcaccat ggatcccccg ggaccggtcc tcgagacgtc tctagagcta gctccggact
     9181 taagactagt gatctgggct gtcctctcca aatgaaatga acttccttat atagaggaag
     9241 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atgtcacatc
     9301 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     9361 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaatgata gcctttcctt
     9421 tatcgcaatg atggcatttg taggagccac cttccttttc tactgtcctt tcgatgaagt
     9481 gacagatagc tgggcaatgg aatccgagga ggtttcccga aattaccctt tgttgaaaag
     9541 tctcaatagc cctttggtct tcccttaatt aagggaagac caaagggcta ttgagagtgc
     9601 aataagcggc gattaactag ttctagagcg gccgccaccg cggtggagct cgaattcgta
     9661 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9721 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9781 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9841 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgccaaca
     9901 tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc
     9961 aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt
    10021 gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat
    10081 gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca
    10141 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
    10201 caaagcaagt ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat
    10261 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
    10321 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
    10381 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
    10441 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
    10501 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
    10561 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
    10621 tcatttcatt tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc
    10681 tcgagtctac catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg
    10741 acatgccggc ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc
    10801 gtaccgagcc gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct
    10861 atccctggct cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct
    10921 ggaaggcacg caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc
    10981 accagcggac gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac
    11041 agggcttcaa gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc
    11101 acgaggcgct cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga
    11161 actggcatga cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg
    11221 tcctgcccgt caccgagatt tgactcgagt ttctccataa taatgtgtga gtagttccca
    11281 gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct
    11341 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
    11401 aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa
    11461 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
    11521 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
    11581 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
    11641 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
    11701 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
    11761 atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
    11821 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
    11881 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
    11941 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
    12001 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
    12061 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
    12121 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
    12181 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
    12241 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
    12301 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
    12361 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
    12421 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
    12481 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
    12541 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
    12601 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
    12661 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
    12721 tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc cttaacgtga
    12781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    12841 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    12901 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    12961 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    13021 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    13081 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    13141 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    13201 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    13261 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    13321 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    13381 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    13441 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    13501 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    13561 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
    13621 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
    13681 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
    13741 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
    13801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
    13861 ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg
    13921 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
    13981 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
    14041 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
    14101 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
    14161 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
    14221 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
    14281 cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
    14341 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
    14401 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
    14461 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
    14521 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
    14581 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
    14641 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
    14701 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
    14761 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
    14821 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
    14881 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
    14941 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
    15001 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
    15061 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
    15121 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
    15181 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc
    15241 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
    15301 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
    15361 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
    15421 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca ggcaacccag
    15481 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
    15541 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
    15601 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
    15661 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
    15721 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
    15781 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
    15841 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
    15901 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
    15961 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
    16021 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
    16081 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
    16141 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
    16201 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
    16261 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
    16321 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
    16381 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
    16441 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
    16501 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
    16561 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
    16621 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga
    16681 cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg
    16741 taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg
    16801 ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc
    16861 gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt
    16921 cgagcgatgc acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat
    16981 cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac
    17041 gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc
    17101 gaactcggca atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc
    17161 ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat
    17221 gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc
    17281 gccagggcgt aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt
    17341 gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt
    17401 caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt
    17461 gttcatggcg taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca
    17521 cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt
    17581 gtgcgacatg tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata
    17641 gcagcggagg ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc
    17701 acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta
    17761 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
//
