LOCUS       sequence-339007-        4850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for 
            protein purification from conditioned medium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Zhang F, Zhu X, Wang P, He Q, Huang H, Zheng T, Li Y, Jia H, Xu L, 
            Zhao H, Colozza G, Tao Q, De Robertis EM, Ding Y
  TITLE     The cytokine FAM3B/PANDER is an FGFR ligand that promotes posterior 
            development in Xenopus.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 May 18;118(20). pii: 2100342118. doi:
            10.1073/pnas.2100342118.
  PUBMED    33975953
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-05-18"; volume: "118(20). pii: 
            2100342118. doi"; pages: " 10.1073/pnas.2100342118"
COMMENT     Sequence Label: pCS2-hFAM 3B-His
FEATURES             Location/Qualifiers
     source          1..4850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             775..792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             802..828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    868..1002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(892..911)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     946..965
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1115..1135)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1115..1133)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1154..1170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1154..1170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1167..1189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1178..1194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1202..1208,1209..1226,1227..1232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1247..1268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1385..1402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1556..2144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1636..1655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2315..3106,3107..3175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2938..2957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3176..3280)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3306..3761)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3443..3464)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3655..3674
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3832..4816
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaggatcc catcgatacc
       61 atgcgcccat tggctggtgg cctgctcaag gtggtgttcg tggtcttcgc ctccttgtgt
      121 gcctggtatt cggggtacct gctcgcagag ctcattccag atgcacccct gtccagtgct
      181 gcctatagca tccgcagcat cggggagagg cctgtcctca aagctccagt ccccaaaagg
      241 caaaaatgtg accactggac tccctgccca tctgacacct atgcctacag gttactcagc
      301 ggaggtggca gaagcaagta cgccaaaatc tgctttgagg ataacctact tatgggagaa
      361 cagctgggaa atgttgccag aggaataaac attgccattg tcaactatgt aactgggaat
      421 gtgacagcaa cacgatgctt tgatatgtat gaaggcgata actctggacc gatgacaaag
      481 tttattcaga gtgctgctcc aaaatccctg ctcttcatgg tgacctatga cgacggaagc
      541 acaagactga ataacgatgc caagaatgcc atagaagcac ttggaagtaa agaaatcagg
      601 aacatgaaat tcaggtctag ctgggtattt attgcagcaa aaggcttgga actcccttcc
      661 gaaattcaga gagaaaagat caaccactct gatgctaaga acaacagata ttctggctgg
      721 cctgcagaga tccagataga aggctgcata cccaaagaac gaagcggaag cggacatcat
      781 caccatcacc actaagatcc ttacccatac gacgtcccag actacgctta atcgagcctc
      841 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
      901 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      961 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1021 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg
     1081 ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     1141 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1201 acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1261 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     1321 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     1381 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     2641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     2821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     2881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     2941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3301 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     3781 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc
     3841 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
     3901 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
     3961 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
     4021 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
     4081 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
     4141 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
     4201 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
     4261 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
     4321 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
     4381 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
     4441 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
     4501 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
     4561 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
     4621 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
     4681 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
     4741 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     4801 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg
//
