LOCUS       sequence-339025-       10012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iRFP670 fluorophore targeted to the plasma membrane using 
            a prenylation motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Harris MJ, Fuyal M, James JR
  TITLE     Quantifying persistence in the T-cell signaling network using an 
            optically controllable antigen receptor.
  JOURNAL   Mol Syst Biol. 2021 May;17(5):e10091. doi: 10.15252/msb.202010091.
  PUBMED    33988299
REFERENCE   2  (bases 1 to 10012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2021-05"; volume: "17(5):e10091. doi"; pages: " 
            10.15252/msb.202010091"
COMMENT     Sequence Label: pHR-iRFP670-CaaX
FEATURES             Location/Qualifiers
     source          1..10012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(24..42)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(25..42)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(332..351)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(565..584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     684..706
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(744..762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        830..934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(935..1003,1004..1795)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1153..1172)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1966..2554
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2455..2474
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2708..2725
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2795..2815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2800..3129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2980..3115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4263..4328
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4458..4478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4903..5037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4940..4959)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4994..5013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5205..5838
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5885..6010
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6507..6740
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6925..6969
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7254..7371
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7524..7931
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8013..8022
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8019..8951
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    9080..9668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9133..9153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9551..9562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9671..9687)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9672..9688)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9778..10011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
       61 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
      121 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
      181 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
      241 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
      301 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      361 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      421 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      481 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      541 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      601 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      661 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      721 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      901 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      961 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1021 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1081 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1141 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1681 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1741 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1801 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1861 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2101 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2341 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2641 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2701 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2761 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     2821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     2941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3121 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     3181 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     3241 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     3301 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     3361 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     3421 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     3481 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     3541 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     3601 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     3661 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     3721 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     3781 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     3841 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     3901 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     3961 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     4021 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     4081 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     4141 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     4201 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     4261 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     4321 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     4381 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     4441 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     4501 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     4561 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     4621 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     4681 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     4741 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     4801 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4861 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4921 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4981 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     5041 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     5101 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
     5161 taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca
     5221 ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     5281 tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg
     5341 ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac
     5401 cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga
     5461 gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta
     5521 cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg
     5581 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
     5641 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5701 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     5821 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     5881 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     5941 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6001 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6061 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     6121 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     6181 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     6241 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     6301 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
     6361 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     6421 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     6481 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     6541 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     6601 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     6661 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     6721 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     6781 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     6841 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     6901 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     6961 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     7021 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     7081 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     7141 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     7201 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
     7261 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     7321 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     7381 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc
     7441 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     7501 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     7561 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     7621 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     7681 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     7741 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     7801 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     7861 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     7921 agtcgcccgg gggggatctg gagctctcga gaattctcac gcgtctgcag gatatcaagc
     7981 ttgcggtacc gcgggcccgg gatccaccgg tcgccaccat ggcgcgtaag gtcgatctca
     8041 cctcctgcga tcgcgagccg atccacatcc ccggcagcat tcagccgtgc ggctgcctgc
     8101 tagcctgcga cgcgcaggcg gtgcggatca cgcgcattac ggaaaatgcc ggcgcgttct
     8161 ttggacgcga aactccgcgg gtcggtgagc tactcgccga ttacttcggc gagaccgaag
     8221 cccatgcgct gcgcaacgca ctggcgcagt cctccgatcc aaagcgaccg gcgctgatct
     8281 tcggttggcg cgacggcctg accggccgca ccttcgacat ctcactgcat cgccatgacg
     8341 gtacatcgat catcgagttc gagcctgcgg cggccgaaca ggccgacaat ccgctgcggc
     8401 tgacgcggca gatcatcgcg cgcaccaaag aactgaagtc gctcgaagag atggccgcac
     8461 gggtgccgcg ctatctgcag gcgatgctcg gctatcaccg cgtgatgttg taccgcttcg
     8521 cggacgacgg ctccgggatg gtgatcggcg aggcgaagcg cagcgacctc gagagctttc
     8581 tcggtcagca ctttccggcg tcgctggtcc cgcagcaggc gcggctactg tacttgaaga
     8641 acgcgatccg cgtggtctcg gattcgcgcg gcatcagcag ccggatcgtg cccgagcacg
     8701 acgcctccgg cgccgcgctc gatctgtcgt tcgcgcacct gcgcagcatc tcgccctgcc
     8761 atctcgaatt tctgcggaac atgggcgtca gcgcctcgat gtcgctgtcg atcatcattg
     8821 acggcacgct atggggattg atcatctgtc atcattacga gccgcgtgcc gtgccgatgg
     8881 cgcagcgcgt cgcggccgaa atgttcgccg acttcttatc gctgcacttc accgccgccc
     8941 accaccaacg cctcgagaaa atgtccaagg atggtaagaa aaagaagaag aagtcaaaaa
     9001 ccaagtgtgt tatcatgtaa gcggccgcga ctctagagtc gacctgcagg catgcaagct
     9061 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     9121 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     9181 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     9241 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     9301 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     9361 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9421 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     9481 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9541 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9601 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     9661 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     9721 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9781 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     9841 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9901 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9961 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc ag
//
