LOCUS       sequence-33903-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, 
            Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo 
            L
  TITLE     Cortical representations of olfactory input by trans-synaptic 
            tracing.
  JOURNAL   Nature. 2011 Apr 14;472(7342):191-6. doi: 10.1038/nature09714.
  PUBMED    21179085
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-04-14- 14"; volume: "472"; issue: "7342"; 
            pages: "191-6"
COMMENT     Sequence Label: pBT140
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             92..112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 ctggctagtt aagcttggta ccaccatggg cccaaagaag aagagaaagg tttcgaattt
      121 actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg
      181 caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat
      241 gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt
      301 tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc
      361 agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct
      421 gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa
      481 cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca
      541 ggttcgttca ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct
      601 ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga
      661 tatctcacgt actgacggtg ggagaatg
//
