LOCUS       sequence-33904-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, 
            Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo 
            L
  TITLE     Cortical representations of olfactory input by trans-synaptic 
            tracing.
  JOURNAL   Nature. 2011 Apr 14;472(7342):191-6. doi: 10.1038/nature09714.
  PUBMED    21179085
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-04-14- 14"; volume: "472"; issue: "7342"; 
            pages: "191-6"
COMMENT     Sequence Label: pBT239
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(67..85,86..108,109..127,128..150,151..169,170..192,
                     193..211,212..234,235..253,254..276,277..295,296..318,
                     319..337)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        370..408
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     405..429
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccctcact aaagggaaca aaagctggag ctgatttaaa taggagatct gattcgagtt
       61 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      121 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      181 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      241 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      301 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac
      361 ccgggtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
      421 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      481 cgatccagcc tccgcggccc cgaattcgag ctcggtaccc ggggatcggg ccgccaccat
      541 ggtgagtaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
      601 ctccatgaac ggccacgagt tcgagatcga g
//
