LOCUS       sequence-339075-       16608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Production of RVdG-CVS-N2c viral vectors for cell-type specific 
            trans-synaptic retrograde labeling..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16608)
  AUTHORS   Sumser A, Joesch M, Jonas P, Ben-Simon Y
  TITLE     Fast, high-throughput production of improved rabies viral vectors 
            for specific, efficient and versatile transsynaptic retrograde 
            labeling.
  JOURNAL   Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848.
  PUBMED    36040301
REFERENCE   2  (bases 1 to 16608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-08-30"; volume: "11:e79848. doi"; pages: " 
            10.7554/eLife.79848"
COMMENT     Sequence Label: CVS-N2c-tdTomato-ChIEF
FEATURES             Location/Qualifiers
     source          1..16608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3319..3328
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3325..4755
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             8037..8048
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(9840..9851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(13047..13065)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13061..13108
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(13136..13152)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13136..13152)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13149..13171)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13160..13176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13184..13190,13191..13208,13209..13214))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13229..13250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13367..13384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13538..14126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13618..13637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14297..15088,15089..15157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14920..14939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15158..15262)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      15289..15745
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15376..15395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15586..15607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15871..15893
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15885..15902
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15886..15902
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        15972..16275
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16276..16479
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     16430..16450
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     16524..16543
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        16524..16542
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcttaaca acaaaaccag agaagaaaaa gacagcgtca attgcaaagc aaaaatgtaa
       61 cacccctaca atggatgccg acaagattgt gttcaaagtc aataatcagg tggtctcttt
      121 gaagcctgag attatcgtgg atcaatatga gtacaagtac cctgccatca aggatttgaa
      181 aaagccttgt atcaccctag ggaaagcccc cgacttgaac aaagcataca aatcagtttt
      241 atcaggcatg aatgccgcca aacttgatcc ggatgatgta tgctcctact tggcagcagc
      301 aatgcagttc tttgagggga catgtccgga agactggacc agctatggaa tcctgattgc
      361 acgaaaagga gataggatca ccccaaactc tctagtggag ataaagcgta ctgatgtaga
      421 agggaattgg gctctgacag gaggcatgga attgacaagg gaccccactg tctctgaaca
      481 tgcatcttta gtcggtcttc tcctgagtct gtacaggttg agcaaaatat caggacagaa
      541 cactggtaac tataagacaa acattgcaga taggatagag cagattttcg agacagcacc
      601 ttttgttaag atcgtggaac accataccct aatgacaact cacaagatgt gtgctaattg
      661 gagtactata ccgaacttca gatttttggc cggaacctac gacatgtttt tctcacggat
      721 tgagcatcta tattcggcaa tcagagtggg cacagtcgtc accgcttatg aagactgctc
      781 aggactggta tcgtttacag ggttcataaa gcagatcaat ctcaccgcaa gggaagcaat
      841 actatatttc ttccacaaga actttgagga agagataaga agaatgttcg agccagggca
      901 agagacagct gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa
      961 gtctccttat tcatcgaatg ctgtcggtca tgtgttcaat ctcattcact ttgttggatg
     1021 ctacatgggt caagtcagat ctctaaatgc gacggttatt gctgcatgtg cccctcatga
     1081 gatgtctgtt ctagggggct atttgggaga ggaattcttc ggaaaaggga catttgaaag
     1141 aaggttcttc agagacgaga aagaacttca agaatatgag gcggctgaac taacaaagtc
     1201 cgacgtggca ctggcagatg acggaaccgt caactctgat gacgaggact atttctctgg
     1261 tgaaaccaga agtccagaag ctgtctatac tcgaatcatg atgaatggag gtcgactgaa
     1321 gagatctcat atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc
     1381 attcgccgaa tttttaaaca agacgtattc gaatgactca taaggagttg attgacaggg
     1441 tgccagaaat ctatagattg tatatatcca tcatgaaaaa aactaacact cctcctttca
     1501 aaccatccca aatatgagca agatctttgt taatccgagt gcaatcagag ccggtctggc
     1561 cgatcttgag atggccgaag agactgttga tctgatcaac agaaacatag aagacaatca
     1621 ggctcatctc cagggagaac ccatagaagt ggacaacctc cctgaggaca tgaagcgact
     1681 tcacctggac gatgaaaaat cgtccaacct tggtgagatg gttagggtgg gagaaggcaa
     1741 gtatcgagag gactttcaga tggatgaggg agaggacccc aacctcctgt tccaatcgta
     1801 cctggataat gttggagtcc aaatagtcag acaaatgagg tcaggagaga gattcctcaa
     1861 gatatggtca cagaccgtag aggaaattgt atcctatgtc acggtcaact ttcctaaccc
     1921 tccaagaagg tcttcggagg ataaatcaac ccagactact ggcagagagc tcaagaagga
     1981 gacaacgtct gctttctctc agagagaaag ccaaccttcg aaagctagga tggtggctca
     2041 agttgcccct ggtcctccag cccttgaatg gtcagccacc aatgaagaag atgatctatc
     2101 agtagaggct gagatcgctc atcagattgc tgaaagcttt tccaagaagt acaagtttcc
     2161 ctcccgatct tcaggaatat tcttgtataa ttttgagcaa ctgaagatga accttgatga
     2221 catagttaaa gaggcaaaaa atgtaccggg cgtgacccgt ctggcccatg atggatccaa
     2281 aatccccctg agatgcgtac tgggatgggt cgctttggct aattccaaaa aattccaatt
     2341 actagtcgag gctgacaagc taagcaaaat catgcaagat gatttgaatc gctatacatc
     2401 ctgctaaccg agttttcgaa ctcagtccct ccagataatg aaaactgaga tgttatggag
     2461 tagacatgaa aaaaacaggc aacaccactg ataaaatgaa cgttctacgc aagatagtga
     2521 aaaaatgtag ggatgaggac actcaaaagc cctctcctgt gtcagcccct ccgtatgacg
     2581 atgacctgtg gcttccacct cctgaatatg tcccgctgaa agaactcaca agcaagaaga
     2641 acatgaggaa cttttgtgtc aacggggagg ttaaagcgtg tagcccaaat ggttactcat
     2701 tcaggatttt gcggcacatt ctgagatcat tcaacgagat atactctggg aatcatagga
     2761 tgattgggtt agtcaaaatt gttgttggac tagctttatc aggagctcca gtacctgagg
     2821 gcatgaactg ggtatacaaa ttgaggagaa cccttatatt ccagtgggct gattccaggg
     2881 gccctcttga aggggaggag ttagattact ctcaagagat cacttgggat gatgatactg
     2941 aattcgtcgg attgcaaata agagtgagcg caagacaatg tcatattcaa ggcaggatct
     3001 ggtgtatcaa cacgaactcg agggcatgtc aactatggtc tgacatgtct cttcagacac
     3061 aaaggtctga agaggacaaa gactcttctc tgcttctaga ataatcagat tatatcctgc
     3121 aagtgtatca cttgtttacc tctggaggag agagcataca ggcttgactc cgatccttgg
     3181 gagcaataga acaaaaaaac acacgttatg gtgccgttaa atcgctgcat tttatcaaag
     3241 tcaagttgat aacctttaca ttttgagcct cttggatgtg aaaaaaacta ttaacatccc
     3301 tcaaaagacc ccgggaaagc caccatggtg agcaagggcg aggaggtcat caaagagttc
     3361 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     3421 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     3481 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     3541 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     3601 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     3661 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     3721 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     3781 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     3841 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     3901 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     3961 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
     4021 accggcagcg gcagctccgg caccgcctcc tccgaggaca acaacatggc cgtcatcaaa
     4081 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     4141 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4201 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     4261 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     4321 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     4381 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     4441 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     4501 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     4561 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     4621 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     4681 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctgta cggcatggac
     4741 gagctgtaca agtaataggc tagacatgaa aaaaactaac actcctccgg taccgccacc
     4801 atgtcgcgga ggccatggct tcttgcccta gcgctggcag tggcgctggc ggccggcagc
     4861 gcaggagcct cgactggcag tgacgcgacg gtgccggtcg cgactcagga tggccccgac
     4921 tacgttttcc accgtgccca cgagcgcatg ctcttccaaa cctcatacac tcttgagaac
     4981 aatggttctg ttatttgcat cccgaacaac ggccagtgct tctgcttggc ttggcttaaa
     5041 tccaacggaa caaatgccga gaagttggct gccaacattc tgcagtggat tacttttgcg
     5101 ctttcagcgc tctgcctgat gttctacggc taccagacct ggaagtctac ttgcggctgg
     5161 gaggagattt acgtggccac gatcgagatg atcaagttca tcatcgagta tttccatgag
     5221 tttgacgaac ctgcggtgat ctactcatcc aacggcaaca agaccgtgtg gcttcgttac
     5281 gcggcctggc tgctgacctg ccctgtcgtt cttatccatc tgagcaacct tacgggtctg
     5341 gcgaacgact ataacaagcg taccatgggt ctgctggtgt cagatatcgg ctgtatcgtg
     5401 tggggcacca cggccgcgct gtccaaggga tacgtccgtg tcattttctt cctgatgggc
     5461 ctgtgctacg gcatctacac attcttcaac gcagccaagg tctacattga ggcgtaccac
     5521 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
     5581 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
     5641 gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt
     5701 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
     5761 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
     5821 gacgaggccg aggctggcgc ggtcgttaac ggaagcggat aagctagcgg cgcctagccg
     5881 gtcatccttt tgacgattcc agtcccgagg ataacctcct ctcgggattg gggggaatct
     5941 ttggatccag tagtcctcct tgaactccgt ccaacagggc agattcaaga gtcataagac
     6001 tttcattaat catttcagtt gatcagacat ggtcgtgtag attctcttaa tacgggagat
     6061 cttctagcag tttcagtgac caacggtgct ttcattctcc aggaactgat accaaaggtt
     6121 gtggacaggc caaggggtac ttcggatgac tctgtgcttg ggcacagaaa gaggtcgtag
     6181 tgtgcccccc gatagcggac tcgacatgaa tcaactaaga aaggcaatct gcctcccatg
     6241 atggacataa gcaatagttc acaaccatct tgcatctcag tgaagtgtac ataactattg
     6301 agggctgggt catctaagca tttcagtcga gaaaaaaact gtagaccaaa agaacaacta
     6361 gcaacacttc tcatccagag acccatatca agatgctaga tccgggagag gtttatgatg
     6421 accctattga tccaattgag tcagaggctg aacccagagg aacccccact gtccccaaca
     6481 tcttgaggaa ctccgactac aatctcaatt ctcctttgat agaggatcct gccaaactaa
     6541 tgttagaatg gttgaagaca gggaacagac cttatcggat gactttgaca gacaattgct
     6601 ccaggtctta caaagttttg aaagattatt tcaagaaagt agatttgggt tctctcaaag
     6661 tgggcggaac tgctgcacag tcaatggttt ctctctggtt gtgtggtgcc cactctgaat
     6721 caaacaggag ccggagatgt ataaccgact tggcccattt ctattccaag tcatccccca
     6781 tagagaagct attgaattgt acgctaggaa acagaggcct gagaatccca ccagaggggg
     6841 tgttaaattg cctcgagagg gtcaattatg acaaggcatt tgggaggtat ctggccaaca
     6901 cgtattcctc ttacttgttt ttccatgtaa tcaccttata catgaatgcc ttagactggg
     6961 aagaggaaaa gaccatcctg gcattatgga aagatataac ctcagtggat accgagaagg
     7021 acttggtcaa attcaaagat caaatatggg gactgttgat tgtgacaaag gactttgttt
     7081 actctcagag ttcaaactgt ctttttgaca gaaactacac actgatgcta aaggatcttt
     7141 tcttgtctcg attcaactcc ttaatgattc tgctttctcc ccctgagccc cgatactcag
     7201 atgacttaat atctcagctg tgccagctat acatcgctgg ggatcaagtc ttgtccatgt
     7261 gtgggaactc cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aacagtttgg
     7321 tccagagggc agagaagttt aggcctctca tccacccctt gggagacttt cctatgttca
     7381 taaaagacaa ggtgaatcaa cttgaaggga cttttggtcc cagtgcaaag aggtttttta
     7441 gggttttaga tcaattcgac aacatacatg acttagtatt tgtgtatggc tgttacagac
     7501 attgggggca cccctatata gattatcgga agggtctgtc gaaactatat gatcaagttc
     7561 acattaagaa agtaatagat aagtcctacc aggagtgttt agcaagtgac ttggccagaa
     7621 ggatcctcag atggggattt gacaagtact ccaaatggta tctagattcg agattccttg
     7681 cccgagacca ccccttgact ccttatgtca agacccaaac atggccaccc aaacatatag
     7741 tagacttggt gggggacaca tggcataagc tcccgatcac gcagatcttt gaaattcctg
     7801 aatcaatgga cccgtcagag atactggatg acaaatcaca ttctttcacc agaacaaggt
     7861 tagcttcttg gctgtcagag aaccgagggg ggcctgttcc tagcgagaag gtcattatca
     7921 cggccctgtc taagccacct gtcaatcccc gagagttttt gaaatctata gacctcggag
     7981 gattgccaga tgaggatttg ataattggcc tcaaaccaaa ggaacgggag ttgaagatcg
     8041 agggccgatt ctttgctcta atgtcatgga atctaagatt atattttgtc atcaccgaaa
     8101 agctcttggc caactacatt ttgccacttt ttgacgcact gactatgaca gacaacctga
     8161 acaaggtgtt caaaaagttg atcgacaggg tcaccgggca agggcttttg gactattcta
     8221 gggtcacata cgcatttcac ctggactatg agaaatggaa caatcatcaa agattggagt
     8281 caacagagga tgtattctct gtcctagatc aggtgtttgg attgaagagg gtgttttcta
     8341 gaacacacga gttttttcag aagtcctgga tctattattc agacagatca gacctcattg
     8401 ggttatggga ggaccaaata tattgcttgg atatgtctaa cggcccaacc tgctggaatg
     8461 gccaagatgg cgggctagag ggcttgcggc agaagggctg gagtctagtc agtttattaa
     8521 tgatagatag agaatctcaa accaggaaca caagaaccaa gatactagct caaggagaca
     8581 accaggttct gtgtcctaca tacatgttgt caccgggatt gtctcaagag gggcttctct
     8641 atgagttaga gagcatatca aggaatgcac tctcaatata ccgagctatc gaggaaggag
     8701 catctaagct ggggctgatc atcaagaagg aagagaccat gtgtagttat gactttctca
     8761 tatatgggaa gaccccctta tttcgaggca acatattggt gcctgaatcc aaaagatggg
     8821 cccgagtctc ttgcatctct aacgaccaaa tagtcaacct cgccaatata atgtcgacag
     8881 tatccaccaa tgcgctgaca gtggcacaac actctcaatc tctgatcaaa cctatgaggg
     8941 attttctgct catgtcagta caggcagttt tccactacct gttgtttagc ccaatcctaa
     9001 aaggcagagt ttataagatt ctgagtgctg aaggggagag ctttctccta gccatgtcgc
     9061 ggataatcta cctagatcct tctttgggag gggtgtctgg aatgtctctc gggaggttcc
     9121 atatacgtca gttctcagac cctgtctctg aagggttgtc attctggaga gagatctggt
     9181 taggctctca tgagtcctgg attcacgcgt tgtgtcagga ggccgggaac cccgatcttg
     9241 gagagagaac actcgagagc ttcactcgcc ttttagaaga tcctactacc ttaaatatca
     9301 aaggaggggc cagccctacc attctactca aggatgctat cagaaaggct ctgtacgacg
     9361 aggtggacaa ggtggaaaat tcagagtttc gagaggcaat cctgctgtcc aagacccata
     9421 gagataactt tatactcttt ttaaaatctg ttgagcctct gttccctcga tttctcagtg
     9481 agctcttcag ttcgtctttc ttgggaatac cggagtcaat cattggactg atacaaaact
     9541 cccggacaat aagaaggcag tttagaaaga gtctctcaag aactttagaa gagtccttct
     9601 acaactcaga gatccacggg attaatcgga taacccagac acctcaaagg gtcggaaggg
     9661 tgtggccttg ctcttcagag agggcagatc tacttaggga gatctcttgg gggaggaaag
     9721 tggtaggcac gacagttcct cacccttccg agatgttgga gttgtttccc aaatcctcca
     9781 tttcctgcac ttgtggagca acagggggag gcaatcctag agtctctgta tcagtactcc
     9841 cgtccttcga tcagtcattt ttctcacggg gccccctaaa gggatacttg ggctcgtcca
     9901 cctccatgtc aacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga
     9961 aaagggctct atcgttaaaa gaatctataa actggttcat caataggaat tccaatttgg
    10021 ctcaaactct aattggaaac atcatgtctc tgacaggccc tgatttccct ctagaagagg
    10081 cccctgtttt caaacggaca gggtcagcct tgcataggtt caagtctgcc agatacagcg
    10141 aaggagggta ttcttctgtt tgccctaacc ttctctccca tatctctgtt agtacagaca
    10201 ctatgtctga tttgacccaa aacgggaaga actatgattt catgtttcag ccattgatgc
    10261 tttatgcgca aacatggaca tcggaactgg tacagaggga tacaagactt agagactcca
    10321 cgtttcactg gcaccttcgg tgcaacagat gtgtgaggcc cattgatgat ataacactgg
    10381 aaacttctca gatcttcgag ttcccggatg tgtcaaaaag gatatccagg atggtttctg
    10441 gagctgtccc tcaatttcag aagcttcctg atatccgtct aagaccaggt gattttgaat
    10501 ccctaagtgg tagagaaaag tctcgccata tagggtcagc tcaggggctc ttatactcaa
    10561 tcttagtagc aattcacgac tcaggataca atgatgggac catcttccct gtcaacatat
    10621 acggcaaagt ttcccccaga gactatttga gagggcttgc aagagggatc ttgatagggt
    10681 cctcgatttg cttcttgaca cgaatgacaa atattaacat taacagacct cttgaattga
    10741 tctcaggggt aatttcctat attctcctga ggctggataa tcatccctct ctgtatataa
    10801 tgcttagaga accgtctctt agaggagaaa tattctctat ccctcagaaa atccccgccg
    10861 cttacccaac cactatgaga gaaggcaaca gatcgatttt gtgttacctc caacacgtgc
    10921 tacgctatga gcgagaggca atcacggcgt ccccggagaa tgactggctg tggatcttct
    10981 cagacttcag aagtgtgaaa atgacgtact tgaccctcat tacctaccag tctcacctcc
    11041 tactccagag ggttgagaga aacttgtcta agagtatgag agctactctg cgacaaatga
    11101 gttccttaat gaggcaagtg ctgggtgggc acggagaaga taccttggag tcagacgatg
    11161 acattcaacg attactaaaa gactctttgc gaaagacaag gtgggtggat caagaggtgc
    11221 gccatgcagc tagaaccatg aatggagatt acagccccga caagaaagta tcccacaagg
    11281 caggatgttc agaatgggtc tgctctgctc aacagattgc cgtctccacc tcagccaacc
    11341 cggcccctgt ctcagagctt gacattaggg ccctctctaa gaggtttcaa aaccccttga
    11401 tctcgggcct gagagtggtt cagtgggcaa ccggtgccca ttataagctt aagcctattc
    11461 tagatgatct aaatgttttc ccatctctct gtcttgtaat tggagacggg tcagggggaa
    11521 tatcaagggc agttctcaac atgtttccag attctaagct tgtgttcaac agcctattgg
    11581 aggtgaatga tctgatggct tccggaacac atccactgcc tccttcagca atcatgagtg
    11641 gaggagatga catcatctcc agagtgatag actttgactc aatatgggag aaaccgtccg
    11701 acctgaggaa ctcggccacc tggagatact tccagtcggt tcaaaaacag gtcaacatgt
    11761 cgtatgacct cattatttgt gatgcagaag ttactgatat tgcatctatc aaccggataa
    11821 ctctgttgat gtctgatttc gcattgtcta tagatggacc actttatctg gtcttcaaaa
    11881 cttacgggac tatgctagta aacccggact ataaagctat tcaacatctg tcaagagcgt
    11941 tcccttcggt cacagggttt gtaacccaag taacttcatc cttttcttct gagctatacc
    12001 tccggttctc taaacgagga aagtttttca gggacgccga gtacttgacc tcttccaccc
    12061 ttcgagagat gagccttgtg ttgttcaatt gcagcagccc caaaagtgag atgcagagag
    12121 ctcgttcctt aaactatcaa gacctggtaa ggggatttcc tgaagagatc atatcaaatc
    12181 cttacaacga aatgatcata actctgattg acaatgatgt agagtccttc ctagtccaca
    12241 agatggtgga tgatcttgag ctacagaggg gaactctgtc taaagtggct atcattatat
    12301 ccatcatgat cgttttttcc aatagagtct tcaacatttc caaacctttg actgacccct
    12361 tgttctaccc cccatctgat cctaaaatcc tgaggcactt caacatatgt tgcagtacta
    12421 tgatgtatct atctaccgct ttaggcgacg tccctaactt cgcaagactt catgacctgt
    12481 ataacagacc tataacttgt tacttcagaa aacaagttat tcgagggaat atttatctat
    12541 cttggagttg gtccgatgat accccagtgt tcaagagagt agcctgtaat tctagcttga
    12601 gtctgtcatc tcactggatc aggttgatct acaagatagt gaagactacc agactcattg
    12661 gcagcataaa agacctatca ggagaggtag aacgacatct tcatgggtat aacagatgga
    12721 tcaccctcga ggatatccga tctagatcat ccctactaga ttacagttgt ttgtaagccg
    12781 gatattaccg aaagcctgtg catgctaaaa ttcttgtatg atgcatcttg aaaaaaacaa
    12841 gatcttgaat ccggacctct ggttgtttga ttgttttttc catctttatt gtttttttgt
    12901 taagcgtggg tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga
    12961 ggacgcacgt ccactcggat ggctaaggga gggcggggat ccggctgcta acaaagcccg
    13021 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
    13081 ctctaaacgg gtcttgaggg gttttttgct gaaagtcgcg cttggcgtaa tcatggtcat
    13141 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    13201 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    13261 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    13321 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    13381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    13441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    13501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    13561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    13981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    14041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    14101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    14161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    14221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    14281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    14341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    14401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacccttg gaggctccag
    14461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    14521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    14581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    14641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    14941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    15001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    15061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    15121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    15181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    15241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta
    15301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
    15361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
    15421 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
    15481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
    15541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
    15601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
    15661 cgatttcggc ctattggtta aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    15721 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
    15781 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    15841 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    15901 gtgagcgcgc ctagttatta atagtaatca attacggggt cattagttca tagcccatat
    15961 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
    16021 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
    16081 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
    16141 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
    16201 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
    16261 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
    16321 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
    16381 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
    16441 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
    16501 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
    16561 attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtc
//
