LOCUS       sequence-339076-       10462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino 
            acids for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Garcia-Beltran WF, Lam EC, St Denis K, Nitido AD, Garcia ZH, Hauser 
            BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, 
            Schmidt AG, Iafrate AJ, Naranbhai V, Balazs AB
  TITLE     Multiple SARS-CoV-2 variants escape neutralization by 
            vaccine-induced humoral immunity.
  JOURNAL   Cell. 2021 Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013.
  PUBMED    33743213
REFERENCE   2  (bases 1 to 10462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Mar 12. pii: S0092-8674(21)00298-1. doi: 
            10.1016/j.cell.2021.03.013."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Delta-18 P.1
FEATURES             Location/Qualifiers
     source          1..10462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3794..4382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3847..3867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4265..4276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4435..4556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4446..4465)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4500..4519
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4671..4717
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4747..4883
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4928..4999
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5044..5240
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5091..5226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5254..6048
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5308..5327)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5918..5937
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6059..6283
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6479..7067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6559..6578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7813..7881,7882..8673)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8031..8050)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8686..8822
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    8974..9045
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9050..9096)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9114..9162
                     /note="synthetic polyadenylation signal"
     enhancer        9243..9546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9548..9750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9701..9721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9884..10359
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 gtgaacttca caaatagaac gcagctccca tccgcttaca cgaactcttt tactcgtgga
       61 gtatactatc ccgataaagt ctttaggtcc agtgtccttc attctactca agaccttttc
      121 ctgccattct tctcaaatgt cacctggttc catgctatcc acgtttctgg caccaacgga
      181 actaaacgat ttgataatcc cgtcttgcca tttaacgatg gcgtatactt tgcctcaacg
      241 gagaagtcca acattatccg gggatggata tttggcacca ccctggactc caagacacag
      301 tctctcctca ttgtgaacaa cgccaccaat gtcgtcatca aagtctgtga atttcaattt
      361 tgtaactatc cctttctcgg ggtttattat cataagaata acaagtcttg gatggagtca
      421 gagttccgcg tttacagttc cgctaacaac tgtacttttg agtacgtttc ccaaccattt
      481 ctcatggatc ttgaagggaa gcaaggcaac ttcaagaatc tcagcgaatt tgtgtttaag
      541 aatatagacg gctacttcaa aatttatagc aagcataccc cgataaatct ggttcgggat
      601 ctccctcaag gcttctccgc gttggaaccc ctcgtggact tgccgattgg catcaacatc
      661 acccggttcc aaacccttct tgcccttcat cgaagctacc tcacgcctgg cgacagtagt
      721 agtggttgga cagcgggcgc agcagcctac tatgtgggat atttgcaacc acgcaccttt
      781 cttctcaaat ataacgaaaa cggaacaatt accgatgccg tagactgtgc cctcgatccc
      841 ctgtccgaaa ctaaatgcac actcaagtcc ttcacagttg agaaaggcat ataccaaacc
      901 agtaacttcc gcgtccaacc caccgaatcc attgttcgat tccctaacat caccaatttg
      961 tgtcctttcg gtgaagtatt taatgccact cgatttgcat cagtttatgc ctggaatcgc
     1021 aagcgaatat caaattgtgt tgcagattat agtgtcctgt ataatagcgc atcattcagc
     1081 acttttaagt gttatggggt cagccctacg aaattgaatg acttgtgttt taccaacgtt
     1141 tacgcagatt ctttcgtcat acgcggcgac gaagtccgac agattgcacc ggggcaaacc
     1201 ggaacgatag cagactataa ttataagttg ccggatgact tcaccggctg cgttatagca
     1261 tggaactcca ataatctcga ttcaaaagtc ggcggcaact ataattatct ttaccgcctg
     1321 tttcgcaagt caaatctgaa gccctttgaa cgtgacatct ccaccgaaat ttatcaagct
     1381 ggcagcaccc catgtaatgg agtgaaaggg tttaactgtt attttcccct tcaatcatac
     1441 ggatttcaac caacatatgg ggtgggctat caaccatatc gggtggtagt cttgagcttt
     1501 gagctgctcc acgcaccggc tacagtttgc ggaccgaaga aaagcactaa cctggtgaag
     1561 aataaatgtg tgaacttcaa ctttaatggg ctcaccggaa caggggtgct gacagaatca
     1621 aacaagaaat ttctcccttt tcaacaattt ggtagagaca ttgccgatac cacagatgcc
     1681 gtccgcgatc cgcaaacact ggaaatcctc gatataacac cctgtagctt cggcggggtc
     1741 agcgtgataa ctcccggaac gaacacatcc aaccaagtgg ctgtcctgta tcaaggtgtc
     1801 aactgtaccg aagtccctgt ggcgatacac gccgaccaac tgacacccac ctggagagtc
     1861 tactcaacag gttccaatgt ctttcaaact agagcgggtt gtctcattgg cgctgagtat
     1921 gtcaataatt catacgaatg tgatattcct attggggcag gaatctgtgc gtcttatcaa
     1981 actcaaacga actcaccacg gcgcgctcgc tcagtagcca gccagtccat aatcgcctat
     2041 accatgagtc tcggggctga gaactccgtg gcttattcaa acaattccat cgcaatccca
     2101 actaacttta ccattagcgt taccacggaa atcttgcccg tcagcatgac taagacgagc
     2161 gtagactgta cgatgtatat ctgtggggat tccaccgagt gtagcaacct cttgcttcaa
     2221 tacgggtctt tctgtactca actcaaccga gcattgaccg gaattgcggt cgaacaagac
     2281 aagaataccc aagaggtgtt cgcacaagta aagcaaatat acaaaacgcc gccaatcaaa
     2341 gattttggcg ggtttaattt tagccaaata ctgccagacc catctaagcc ttcaaaacgc
     2401 agctttatcg aggatctgct gttcaacaaa gttacgttgg ctgacgcggg tttcattaag
     2461 cagtatgggg attgtttggg agacattgcg gcccgcgacc ttatctgtgc tcaaaaattc
     2521 aatgggttga cagttctgcc ccctctgctc actgatgaga tgattgcaca gtatacatca
     2581 gcattgttgg caggaaccat tacatccggc tggacttttg gcgcgggtgc ggcccttcaa
     2641 atacctttcg ctatgcaaat ggcttacaga ttcaatggga ttggtgtcac gcaaaatgta
     2701 ttgtatgaaa atcagaagct gatcgctaat caatttaaca gcgcgattgg caagatccaa
     2761 gattcactgt caagcacagc gtccgccctc ggcaaactcc aagatgttgt gaatcaaaac
     2821 gcccaggccc tgaataccct cgtgaagcaa ctgagttcta acttcggcgc aatctccagc
     2881 gtcctcaacg acatattgtc cagacttgat aaggtggaag ccgaggtgca aattgatcgg
     2941 ctcatcacag gcaggctcca gtcactccaa acttatgtga cacaacagtt gatccgggct
     3001 gctgagatac gagcaagcgc caacctggcc gccattaaaa tgtcagagtg cgtgctcggg
     3061 cagtccaaac gggttgactt ttgtggcaag ggctatcact tgatgtcctt cccccaatcc
     3121 gccccacatg gagtggtctt tctccacgtc acttatgtcc ctgctcaaga gaagaacttt
     3181 acgacagctc cagcaatctg tcacgatggc aaagcgcact ttcccaggga aggcgttttc
     3241 gtcagtaatg gcacgcattg gtttgtgacc caaaggaatt tctatgaacc ccaaatcata
     3301 accactgaca atacatttgt ctcaggcaac tgtgatgtgg tcattgggat cgtgaacaac
     3361 acggtatatg atcccctgca acccgagttg gatagcttca aagaggaatt ggataaatac
     3421 tttaagaatc atacttcacc cgatgttgat ctcggggaca tatcaggaat aaatgcaagt
     3481 ttcgtcaaca ttcagaaaga gattgatcgc cttaatgagg tcgccaagaa cttgaatgaa
     3541 tctttgatcg acttgcaaga actgggcaaa tacgaacaat atattaaatg gccctggtac
     3601 atttggttgg gctttatcgc cggacttata gctatcgtta tggtcacaat tatgttgtgc
     3661 tgtatgacta gctgttgttc ctgcttgaaa ggatgttgca gttgcggatc ttgttgtaaa
     3721 taaaggatcc tctagaagtt gtctcctcct gcactgactg actgatacaa tcgatttctg
     3781 gatccgcagg cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3841 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3901 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3961 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4021 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4081 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     4201 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4261 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4321 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4381 gcctgtctag cttgactgac tgagatacag cgtaccttca gctcacagac atgataagat
     4441 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4501 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4561 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4621 gcaagtaaaa cctctacaaa tgtggtattg gcccatctct atcggtatcg tagcataacc
     4681 ccttggggcc tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc ggattgctat
     4741 ctaccggcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac
     4801 atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct
     4861 ccttaccaga aatttatcct taaggtcgtc agctatcctg caggcgatct ctcgatttcg
     4921 atcaagacat tcctttaatg gtcttttctg gacaccacta ggggtcagaa gtagttcatc
     4981 aaactttctt ccctccctaa tctcattggt taccttgggc tatcgaaact taattaagcg
     5041 atctgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5101 taactccgcc cagttccgcc cattctccgc cccatcgctg actaattttt tttatttatg
     5161 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5221 gaggcctagg cttttgcaaa ggaggtagcc aacatgattg aacaagatgg attgcacgca
     5281 ggttctcccg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5341 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5401 aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg
     5461 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5521 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5581 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5641 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5701 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5761 ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt gacccacggc
     5821 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5881 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5941 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6001 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctagta tgtaagccct
     6061 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6121 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6181 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6241 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggttaatta accagtcaag
     6301 tcagctactt ggcgagatcg acttgtctgg gtttcgacta cgctcagaat tgcgtcagtc
     6361 aagttcgatc tggtccttgc tattgcaccc gttctccgat tacgagtttc atttaaatca
     6421 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6481 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6541 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6601 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6661 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6721 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6781 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6841 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6901 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6961 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7021 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7081 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7141 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7201 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7261 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcatttaaa tttccgaact
     7321 ctccaaggcc ctcgtcggaa aatcttcaaa cctttcgtcc gatccatctt gcaggctacc
     7381 tctcgaacga actatcgcaa gtctcttggc cggccttgcg ccttggctat tgcttggcag
     7441 cgcctatcgc caggtattac tccaatcccg aatatccgag atcgggatca cccgagagaa
     7501 gttcaaccta catcctcaat cccgatctat ccgagatccg aggaatatcg aaatcggggc
     7561 gcgcctggtg taccgagaac gatcctctca gtgcgagtct cgacgatcca tatcgttgct
     7621 tggcagtcag ccagtcggaa tccagcttgg gacccaggaa gtccaatcgt cagatattgt
     7681 actcaagcct ggtcacggca gcgtaccgat ctgtttaaac ctagatattg atagtctgat
     7741 cggtcaacgt ataatcgagt cctagctttt gcaaacatct atcaagagac aggatcagca
     7801 ggaggctttc gcatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7861 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7921 cagttgggtg cgcgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7981 agttttcgcc ccgaagaacg ctttccaatg atgagcactt ttaaagttct gctatgtggc
     8041 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8101 cagaatgact tggttgagta ttcaccagtc acagaaaagc atcttacgga tggcatgaca
     8161 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8221 ctgacaacga ttggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8281 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8341 gacaccacga tgcctgtagc aatggcaaca accttgcgta aactattaac tggcgaacta
     8401 cttactctag cttcccggca acagttgata gactggatgg aggcggataa agttgcagga
     8461 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8521 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8581 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8641 gagataggtg cctcactgat taagcattgg taaccgattc taggtgcatt ggcgcagaaa
     8701 aaaatgcctg atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcaacgg
     8761 atacggcttc cccaacttgc ccacttccat acgtgtcctc cttaccagaa atttatcctt
     8821 aagatcccga atcgtttaaa ctcgactctg gctctatcga atctccgtcg tttcgagctt
     8881 acgcgaacag ccgtggcgct catttgctcg tcgggcatcg aatctcgtca gctatcgtca
     8941 gcttaccttt ttggcagcga tcgcggctcc cgacatcttg gaccattagc tccacaggta
     9001 tcttcttccc tctagtggtc ataacagcag cttcagctac ctctcaattc aaaaaacccc
     9061 tcaagacccg tttagaggcc ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa
     9121 aaaaccaaca cacatccatc ttcgatggat agcgatttta ttatctaact gctgatcgag
     9181 tgtagccaga tctagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     9241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     9301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     9361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     9421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     9481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     9541 accatgctga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     9601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     9661 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     9721 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat cgcctggaga
     9781 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctcccctcg
     9841 aagcttacat gtggtaccga gctcggatcc tgagaacttc agggtgagtc tatgggaccc
     9901 ttgatgtttt ctttcccctt cttttctatg gttaagttca tgtcatagga aggggagaag
     9961 taacagggta cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg
    10021 ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt
    10081 tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac
    10141 agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat
    10201 aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat
    10261 tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag ctaggccctt
    10321 ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc
    10381 tgtgtgctgg cccatcactt tggcaaaggc ggccgccatg tttgtgttcc tcgttctcct
    10441 gcccctcgtt tcttcccaat gc
//
