LOCUS       sequence-33909-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Zhang H, Fire AZ
  TITLE     Cell autonomous specification of temporal identity by Caenorhabditis
            elegans microRNA lin-4.
  JOURNAL   Dev Biol. 2010 Aug 15. 344(2):603-10.
  PUBMED    20493184
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2010 Aug 15. 344(2):603-10."
COMMENT     Sequence Label: pHZ081
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(190..294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     362..380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(458..477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     577..599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 aacgttcttc ggggcgaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
       61 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      121 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      181 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      241 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      301 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
      361 aaataggcgt atcacgaggc ccttaaggcc gcctgatgcg gtattttctc cttacgcatc
      421 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
      481 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      541 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      601 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggccc aagcgaggac aattctcatc
      661 gttcgcctaa tttctttgct tattttttac tagttttcct tcctcctcta tatattttta
      721 tgttttcttt taaaccttcc tccatatttc tgaattgaat gttgttgcat attgtgtgtc
      781 cttatttcat ttccatcatc cttttgtgtg tttccccctt ccaccaatt
//
