LOCUS       sequence-339093-        9753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sequence provided for PE-Nuclease mRNA transcription, suitable for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, 
            Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, 
            Thomas PQ
  TITLE     Optimized nickase- and nuclease-based prime editing in human and 
            mouse cells.
  JOURNAL   Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 
            10.1093/nar/gkab792.
  PUBMED    34534334
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792."
COMMENT     Sequence Label: pCMV_T7-PE2-Nuclease
FEATURES             Location/Qualifiers
     source          1..9753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             701..721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             722..4822
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6995..7015
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7024..7041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7064..7081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7070..7294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7365..7381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7365..7381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7378..7400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7389..7405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7413..7419,7420..7437,7438..7443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7458..7479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7596..7613)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7767..8355)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7847..7866)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8526..9317,9318..9386))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9149..9168
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9387..9491)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9570..9589)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      601 aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc
      661 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
      721 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
      781 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
      841 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
      901 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
      961 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     1021 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     1081 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     1141 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     1201 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     1261 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     1321 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     1381 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     1441 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     1501 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     1561 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     1621 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     1681 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     1741 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     1801 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     1861 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     1921 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     1981 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     2041 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     2101 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     2161 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     2221 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     2281 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     2341 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     2401 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     2461 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     2521 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     2581 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     2641 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     2701 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     2761 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     2821 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     2881 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     2941 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     3001 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     3061 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     3121 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     3181 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     3241 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     3301 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     3361 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     3421 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     3481 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     3541 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     3601 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     3661 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     3721 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     3781 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     3841 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     3901 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     3961 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     4021 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     4081 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     4141 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     4201 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     4261 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     4321 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     4381 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     4441 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     4501 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     4561 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     4621 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     4681 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcacgaaaga
     4741 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     4801 cctgtctcag ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac
     4861 accaggaaca agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcag
     4921 caccctaaat atagaagatg agtatcggct acatgagacc tcaaaagagc cagatgtttc
     4981 tctagggtcc acatggctgt ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg
     5041 actggcagtt cgccaagctc ctctgatcat acctctgaaa gcaacctcta cccccgtgtc
     5101 cataaaacaa taccccatgt cacaagaagc cagactgggg atcaagcccc acatacagag
     5161 actgttggac cagggaatac tggtaccctg ccagtccccc tggaacacgc ccctgctacc
     5221 cgttaagaaa ccagggacta atgattatag gcctgtccag gatctgagag aagtcaacaa
     5281 gcgggtggag gacatccacc ccaccgtgcc caacccttac aacctcttga gcgggctccc
     5341 accgtcccac cagtggtaca ctgtgcttga tttaaaggat gcctttttct gcctgagact
     5401 ccaccccacc agtcagcctc tcttcgcctt tgagtggaga gatccagaga tgggaatctc
     5461 aggacaattg acctggacca gactcccaca gggtttcaaa aacagtccca ccctgtttaa
     5521 tgaggcactg cacagagacc tagcagactt ccggatccag cacccagact tgatcctgct
     5581 acagtacgtg gatgacttac tgctggccgc cacttctgag ctagactgcc aacaaggtac
     5641 tcgggccctg ttacaaaccc tagggaacct cgggtatcgg gcctcggcca agaaagccca
     5701 aatttgccag aaacaggtca agtatctggg gtatcttcta aaagagggtc agagatggct
     5761 gactgaggcc agaaaagaga ctgtgatggg gcagcctact ccaaagaccc ctcgacaact
     5821 aagggagttc ctagggaagg caggcttctg tcgcctcttc atccctgggt ttgcagaaat
     5881 ggcagccccc ctgtaccctc tcaccaaacc ggggactctg tttaattggg gcccagacca
     5941 acaaaaggcc tatcaagaaa tcaagcaagc ccttctaact gccccagccc tggggttgcc
     6001 agatttgact aagccctttg aactctttgt cgacgagaag cagggctacg ccaaaggtgt
     6061 cctaacgcaa aaactgggac cttggcgtcg gccggtggcc tacctgtcca aaaagctaga
     6121 cccagtagca gctgggtggc ccccttgcct acggatggta gcagccattg ccgtactgac
     6181 aaaggatgca ggcaagctaa ccatgggaca gccactagtc attctggccc cccatgcagt
     6241 agaggcacta gtcaaacaac cccccgaccg ctggctttcc aacgcccgga tgactcacta
     6301 tcaggccttg cttttggaca cggaccgggt ccagttcgga ccggtggtag ccctgaaccc
     6361 ggctacgctg ctcccactgc ctgaggaagg gctgcaacac aactgccttg atatcctggc
     6421 cgaagcccac ggaacccgac ccgacctaac ggaccagccg ctcccagacg ccgaccacac
     6481 ctggtacacg gatggaagca gtctcttaca agagggacag cgtaaggcgg gagctgcggt
     6541 gaccaccgag accgaggtaa tctgggctaa agccctgcca gccgggacat ccgctcagcg
     6601 ggctgaactg atagcactca cccaggccct aaagatggca gaaggtaaga agctaaatgt
     6661 ttatactgat agccgttatg cttttgctac tgcccatatc catggagaaa tatacagaag
     6721 gcgtgggtgg ctcacatcag aaggcaaaga gatcaaaaat aaagacgaga tcttggccct
     6781 actaaaagcc ctctttctgc ccaaaagact tagcataatc cattgtccag gacatcaaaa
     6841 gggacacagc gccgaggcta gaggcaaccg gatggctgac caagcggccc gaaaggcagc
     6901 catcacagag actccagaca cctctaccct cctcatagaa aattcatcac cctctggcgg
     6961 ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaacc
     7021 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     7081 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7141 actcccactg tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt
     7201 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7261 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7321 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7381 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7441 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7561 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7621 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7681 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7741 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7801 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7861 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7921 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7981 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8041 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8101 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8161 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8221 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8281 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8341 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8401 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8461 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8521 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8581 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8641 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8701 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8761 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8821 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8881 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8941 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9001 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9061 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9121 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9181 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9241 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9301 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9361 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9421 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9481 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     9541 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9601 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9661 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9721 tttgcgctgc ttcgcgatgt acgggccaga tat
//
