LOCUS       sequence-339094-       13528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CHO expression studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13528)
  AUTHORS   Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, 
            Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II
  TITLE     Approaches to Controlled Co-Amplification of Genes for Production of
            Biopharmaceuticals: Study of the Insertion and Amplification 
            Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary
            Cells during Co-Expression of Compatible Pair of Plasmids.
  JOURNAL   Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 
            10.1007/s10517-017-3776-0. Epub 2017 Jul 18.
  PUBMED    28726207
REFERENCE   2  (bases 1 to 13528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10517-017-3776-0"; journalName: "Bull Exp Biol Med"; date:
            "2017-06"; volume: "163"; issue: "2"; pages: "245-249"
COMMENT     Sequence Label: p1.2-GS-eGFP
FEATURES             Location/Qualifiers
     source          1..13528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1906..2027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1997..2016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2078..2095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2249..2837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2329..2348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3008..3799,3800..3868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3631..3650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3869..3973)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      8511..8520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8517..8519,8520..8522,8523..9236)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8517..8519,8520..8522,8523..9233)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8562..8583)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8823..8842)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9170..9191
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(join(13524..13528,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 acgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttggat atccattttc
      361 gcatcgatgc ttccaccatg gccacctcag caagttccca cttgaacaaa ggcatcaagc
      421 agatgtacat gaacctgccc cagggcgaga agatccaact catgtatatc tgggttgatg
      481 gtaccgggga agggctacgc tgcaagaccc gtactctgga ctgtgacccc aagtgtgtag
      541 aagagttacc cgagtggaac tttgatggtt ctagtacgtt tcagtctgaa ggctccaaca
      601 gcgacatgta cctccatcct gtggccatgt ttcgagaccc cttccgcaga gaccccaaca
      661 agctggtgtt ctgcgaagta ttcaagtata accggaagcc cgcagagacc aacctgaggc
      721 acagctgtaa gcgtataatg gacatggtga gcagccagca cccctggttt ggaatggaac
      781 aggagtatac tctcatggga acagacggcc accctttcgg ctggccttct aatggcttcc
      841 ctggacccca aggaccctat tactgcggtg tgggagctga caaggcttat ggccgagata
      901 tcgtggaggc tcactaccgg gcctgcttgt atgctggaat caagatcaca gggacaaatg
      961 ccgaggttat gcctgcccag tgggaattcc agataggacc ctgcgaaggg atccgcatgg
     1021 gagatcatct ctgggtagcc cgttttatct tgcatcgggt atgcgaagac tttggggtga
     1081 tagcaacctt tgaccccaag cccattccag ggaactggaa tggggcaggc tgccacacca
     1141 actttagcac caaggccatg cgggaggaga atggtctgag gtgcattgag gaggccattg
     1201 ataaactgag caagaggcac cagtaccaca tccgtgccta cgaccccaag gggggcctgg
     1261 acaacgcccg ccgtctgact ggattccacg aaacctccaa catcaacgac ttttccgctg
     1321 gcgttgccaa ccgcagcgcc agtatccgca ttccccggat tgtcggccag gagaagaagg
     1381 gttactttga agaccgtcgg ccttctgcca attgcgaccc ctatgcggtg acggaagcca
     1441 tcgtccgcac gtgtctcctc aacgaaactg gcgacgagcc cttccaatac aagaactaag
     1501 cggactcgac ttccagtgat cttgagccct tcctagttca ccccactccc aactgttccc
     1561 tctcccactg gtccccactg taactcaaaa ggatggaata ccaaggtctt tttattcctt
     1621 gcgcccagtt aatttttgcc tttattggtc agaatagagg ggtcaggttc ttaatctcta
     1681 cacacccaac cccttctttc ctagctatcg ccttcttgac gagttcttct gagcgggact
     1741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1861 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     2041 ccgtcgacct ctaggcgcgc catggctctt ccgcttcctc gctcactgac tcgctgcgct
     2101 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2161 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2221 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2281 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2341 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2401 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2461 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2521 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2581 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2641 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2701 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2761 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2821 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2881 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2941 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3001 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3061 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3961 taggggttcc gcgcacattt ccccgaaaag tgccacctgc atctcatcac ggtcacgcat
     4021 ggctgcgccc gcgcagcgcc ccccgacccc cccccgatcc ccggaacgcc agccgccatc
     4081 caccgcccgc agcccccgga gcccgcggac cccgaccccc cgccgctcgc ccgccgttgc
     4141 gccgctctct gcgggggggc tagggggccg cggggggaag gccacgcccc ctccactttt
     4201 tccaggaatg cgcggcatga cccgcccccc ccatgacccg ccaacacccc cccgatgccc
     4261 cccgggggtc tttcctgggg ggcctttgtc agggttgcct gtgtcaccca cgggcccctt
     4321 ggccacgctt gcctgggtca ccccggagcc ccgggtcacg tttgccgggg tcagccctgg
     4381 ggcgcttctt cacgctccat tgccatgcag aattcactag tgattagaat cccacgttgt
     4441 gcatagaaac agatgcaggc aaaacatcca cacatataaa acaaaaaatt aaaaccaata
     4501 aaactcctaa acttttggtc tttcttgaat cttcaatccc tcaggttatg aaataatcat
     4561 ttatgcagtc aaaaatttgc cattcttgtt gccaggtgtg gtgatgattc ggggaagcag
     4621 aagcaggcag atctctgtga atgaggccag cctggtctac aaagtgagtc ccaggacagt
     4681 caggcctgtt acacagagaa accttgaaaa aaaaaagata atatgtactg ttgtattacc
     4741 ccaatatata aggctaaacc attagaagca caacactgtt aagtacggaa aataatatct
     4801 agtgtggtac agttactact actataatac actaatatag ctgtgggaaa ctagttccaa
     4861 agatgaatta ctaaccagtg tttccaagga aataaatgaa agcagagaga ttagttctat
     4921 tgctagtgtt tcattttcgt atatttctta caatttctct tgttacaaat aggcactagg
     4981 gtatcaagat aattttaacg actggctgag aaccctagaa aatctctgtg aaaaagggat
     5041 ttgtgaaatg agagagggta atgtggccat tatagaaaag gcttttgtgt gccttgcatg
     5101 catagaccct gtgtttgatc tcttaacacc ctccttgacc agaaaaagct tctgtggata
     5161 gaaaatgatt agttatatat acttttaggg aaacgtagtt ctggattctt tggttacaat
     5221 taacagaatt aagtgcaaac aaagccagaa acctcctgat aaatgagaaa acctgcttgt
     5281 agaaggttgt aaggctctgt aatataggaa ttaggagaaa agaaacctgt gtggtggggc
     5341 acgtctgtaa tcccagcatt gggaagtaga ggtagaagat tagaaatcaa aggccagcct
     5401 cagcaacaca gtgagtttga ggccaccctg aactacatca ggttctgtct cctttctttt
     5461 tttttttttt cttttctttt tttggtttct ctgtgtagtt ttggagccta tcctggcact
     5521 agctctgaag agcaggctgg cctcgaactc agagatcagc cagcctctgc tgggattaaa
     5581 ggtatgcacc accaacgccc caggttttgt ctcaaacaaa caaaaataac atcaggaggt
     5641 ggtgagaggg ctcagtggtc acaggcattc tctgcaaagc ctgactctga gttggatcct
     5701 ttagagctac atggttgagg gaagagaact gactcctgga aggtgtcctc tggtccccac
     5761 acatagctat acacagcatg tgcattcaca cacactaaat aatgctattt ttaaaaaaat
     5821 taaaaacaac aacagtttgg gttgtgaaaa ctagaactag ataataggta agaatcaagt
     5881 atcatgtaaa tttgctttca actcatccca aaatttgttt tatatttcag tttttttcct
     5941 tcctagcttg actgtggagt cttgtccgga agcaaatagt tcctttgcag atcccacatg
     6001 tggacaccgg acagtaggtc ctcaaatgct ccttattagg ttggttcaat aatatcaatt
     6061 gtttgttact aggcagtgat gttgtacatc tggaggagat ctcttgagcc cataatcagg
     6121 ttattaggaa taaatactct aaggctaaaa atgtagctta gtgataagag tgcttgcctg
     6181 gtgtgctgag accctcggtt ccatctccac aaccccatat tccattacaa aatacctttt
     6241 caccgtccct agcattaaga aacaaaacaa caaagaagtt tttctttctt ctgagatcct
     6301 gcccggagag gcatttaaaa ctggccaggg ccaaaaaaaa aaaaaaaaga aaaaaaagaa
     6361 aagaaaacag gctagggccg gcatggtggc gcacgccttt aatcccagca cgcaggaggc
     6421 agaggcaggg cggatctctg tgagtttgag gtcagcctgg tctacctagt gagtttcagg
     6481 gcacccaggg ctaaagagac tgtctcaaaa acaaaacagc cacacaatca gaaccacagc
     6541 aaaacgcagt tatgatcctt ggaactgtag gaatgacaag catttaaata ataggacgag
     6601 ccatttttga gaagctctga tttcacaagt gtcagggatg ggctctgggc gagtaagatt
     6661 gctaatgctg gcctctaaat gagaccacgt ggagttgatt agattctttt catgttcctc
     6721 gtgctctatc aaataactgt acccaaatac acacacacac acacacacac aatgcgcgca
     6781 cacacaaaat ccttttttag cttaagaagc ccagaatcag aagtaaagct aactgtggga
     6841 cttaagtatt attctgaacg gaactcccag ggcgtgaagc gcgcttcagg cttccagaga
     6901 agcagctggc gctggatgga atgaaccaag aggccagcac aggggcagat ccgtcgagct
     6961 ctcggccacc gagctgagcc cttaggttct ggggctggga agggtcccta ggattgtgca
     7021 cctctcccgc gggggacaag cagggggatg gcggggctga cgtcgggagg tggcctccac
     7081 gggaagggac acccggatct cgacacagcc ttggcagtgg agtcaggaag ggtagggaca
     7141 gattctggac gccctcttgg ccagctcctc accgccccac ccccgactgg agccgagagt
     7201 aattcataca aaaggaggga tcgccttcgc ccctgggaat cccagggacc gtcgctaaat
     7261 tctggccggc ctcccagccc ggaaccgctg tgcccgccca gcgcggcggg aggagcctgc
     7321 gcctagggcg gatcgcgggt cggcgggaga gcacaagccc acagtccccg gcggtggggg
     7381 aggggcgcgc tgagcggggg cccgggagcc agcgcggggc aaactgggaa agtggcgtcg
     7441 tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtataaaagt gcggtagtcg
     7501 cgttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg
     7561 gcctccgcgg gcccgggctc cctcctttga gcggggtcgg gccgccgtgc gggtgtcgtc
     7621 ggccgggctt ctctgcgagc gttcccgccc tggatggcgg gctgtgcggg agggcgaggg
     7681 ggggaggcct ggcggcggcc ccggagcctc gcctcgtgtc gggcgtgagg cctagcgtgg
     7741 cttccgcccc gccgcgtgcc accgcggccg cgctttgctg tctgcccggc tgccctcgat
     7801 tgcctgcccg cggcccgggc caacaaaggg agggcgtgga gctggctggt agggagcccc
     7861 gtagtccgca tgtcgggcag ggagagcggc agcagtcggg ggggggaccg ggcccgcccg
     7921 tcccgcagca catgtccaac gccgcctgga cgggtagcgg cctgtgtcct gataaggcgg
     7981 ccgggcggtg ggttttagat gccgggttca ggtggccccg ggtcccggcc cggtctggcc
     8041 agtaccccgt agtggcttag ctccgaggag ggcgaggccc gcccgcccgg caccagttgc
     8101 gtgcgcggaa agatggccgc tcccgggccc tgtagcaagg agctcaaaat ggaggacgcg
     8161 gcagcccggc ggagcggggc gggtgagtca cccacacaaa ggaagagggc cttgcccctc
     8221 gccggccgct gcttcctgtg accccgtggt gtaccggccg cacttcagtc accccgggcg
     8281 ctctttcgga gcaccgctgg cctccgctgg gggaggggat ctgtctaatg gcgttggagt
     8341 ttgctcacat ttggtgggtg gagactgtag ccaggccagc ctggccatga aagtaattct
     8401 tggaatttgc ccattttgag tttggagcga agctgattga caaagctgct tagccgttca
     8461 aaggtattct tcgaactttt tttttaaggt gttgtgaaaa cctcgaggcc gccaccatgg
     8521 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     8581 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     8641 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     8701 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     8761 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     8821 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     8881 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     8941 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     9001 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     9061 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     9121 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     9181 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcta
     9241 gcatattacc cctaacacct gccaccccag tcttaatcag tggtggaaga acggtctcag
     9301 aactgtttgt ctcaattggc catttaagtt taatagtgaa agactggtta atgataacaa
     9361 tgcatcggaa aaccttcagg aggaaaggag aatgttttgt ggaacatttt tgtgtgtgtg
     9421 gcagttttaa gttattagtt ttcaaaatca gtacttttta atggaaacaa cttgaccaaa
     9481 aatctgtcac agaattttga gacccattaa aatacaagtt taatgagaag tctgtctctg
     9541 ttaatgctga agtcattact aagtgcttag cttagcaagg tatgtggatg cccatttgtg
     9601 ttccaaggga ttggactgtt catcaggacc cagagctgag tttcaagggc tcaagagatg
     9661 gcttattacc tgtgggtgtc ttgaaggttc tggttgggac aaattaggaa tgtttttggc
     9721 agacatggtg actacccttc atctgggtga gttcagttga tttgtcttga gcctttgggg
     9781 tttacacaag taaatgacat catacagtta gtgtattgtt agtgaatatt aatatatgag
     9841 gcaggctttg ctctagcaat tttagaacta gttttcagga aagggttcat cttgtgcatt
     9901 ggatgtctga ttctatcact tagagtttaa actgaaagtg ctcaagaggt tttatttagg
     9961 ctgggatata aataagcctt tctgtagctt gtaatggtat caggaattta aaaggccatc
    10021 tggggcacaa agattaagca gaaaaggtag aaggtgagat tgggggactt tgagtacttc
    10081 acacacttta atgtgtgggt gctttagtgc atatagtaca actgccagat aagggcatcc
    10141 acatctgatt gtttggaagg caccttgtgg tttctgggaa ttcagaattg ggagaaaaag
    10201 tgctcccaac cgctgaagcc cttggtaatc tgcagggtgt ttatttagca ggagataagg
    10261 acaaaaagtt atagtgtgga gttggttgag ttggtagatg tcattacaac aggtggtctt
    10321 aaattgggtt aggagtcact ttgaaatacc tgggccataa gcaaagtggc attttcacct
    10381 ttcaggagaa actggtacac ttatccattc tatagtgcat gcttgttcaa ttgggctgat
    10441 gactaaaccg gtgactaaag gtttgtcagt ataaatggat gggttgtagg cagacggtga
    10501 ggaattacta tacctgcaag gagtcattgc ctgatctgcc tggaaagggg caggattgag
    10561 tctcagaagg tgtacaccat aggatatgga aaaatttgtc acgcctagca ttcaacttag
    10621 tggtgtagcg ccacctactg gcactttaaa agcttagcat agaggagcat gtgtgttagg
    10681 agctcggatg ggatccaggg cctcaaggtt tgcatgtaaa taaaagccct ttaccaaatt
    10741 aactacatac cagcatacat cagtccttta gtgttgaaaa acagaagtga aagctaatat
    10801 atatagtgct tgctttattt aagtctagct gattacgtgt ttggttgcca gtgtgactag
    10861 tctggagttg aatttgtcct cagacacgta aaatggaatt tgggattcac aacactctag
    10921 tatgagggac ctaatggcct gtaccaggca caaacgtgtc tataaactac acaaaacgaa
    10981 ggaatttaca ggaattagga aggtattctt aacattaaaa cattatgggc attttaaaaa
    11041 aagctttgac aggatttctt tgtcatggct gtcctggagc tagttgtgta gaccaggctg
    11101 ggctgaaatc ttgtctgcct gcctggcttg gacacttttt tattatgtat acaacattct
    11161 gcttccatgt atatctgcac attagaagac ggcaccagat ctcctaatgg atggttgtga
    11221 gccaccatgt ggttgctggg aattgaactc aggacctctg gaagagcagt gctcttaacc
    11281 tctgagccat ctccagcccc agcttgggca catttttaat ggctgggaaa tcaaaccccc
    11341 taggccttct gtcagtaatg aagggctttt ggctaccgag agtaggattt aaggttattc
    11401 ggagctgcag gtctgcctca gtgcaggttt gggagtccag catcttagaa aatgcagtga
    11461 agccaagctg agctatattt tgtttaaaaa aaaaataagt gggtaaagtg ctgctgagcc
    11521 tgatgaccaa gctgggacac aagtagaaga acataggcca atgctctata ttaaaagcat
    11581 gggtcatttt taatgctctt gagaaggcta tgcctacact actctcagcc accgcagcgt
    11641 gtttaaatta aactagtttg gaaattttct ttgggggtaa gctatttaac ctagtgcctt
    11701 ggcaggtata ctactgaact ctcctcctca ttcctttttg ttttttaaga atttcagtca
    11761 ggctcaggca gcccttaaac ttgtgattaa gcctgagaac agttacgatt atgagcctat
    11821 tagtataccg atcaatatgt gaattttttt gggatggggg tcaggcctcc ctgcctccca
    11881 aatactggga ctaaaggctg caccaccaca acctggctct tgaaatactt ttctacattt
    11941 tttggggggc atgggtggga gagcagggtt tctctgtatt agccctggct ctcctggaac
    12001 tctgtagacc aggctattct tgagctcaga ttagcctgtc tctgcctcct aaattctggg
    12061 attaaaggtg tgtgctactg ctgcctggct acaaagacat tttttttttt cttaaattta
    12121 aaaacaaaag tggttctttt agaagggtgg ttggtgttgg cacatactcc aagcactcag
    12181 gttttgagtt tgtcccagga atgaagactg cattactgcc gcccctccct ggtaagggct
    12241 acacagagaa atcctatttg gagcctgtcc tggtaactcg ctctgtagac caggctggcc
    12301 tcgaactcaa gagaaccacc tgcctctgaa tgctggtatt aagggcaggc accaccaaca
    12361 cccagcctaa aaaatgtctt ttttttaaag attttttttt tttttttaca gaataaacat
    12421 tctgtttaca atattctgct tctatgtata tctgcacact agaagagggc acccgatctc
    12481 ataatggatg gttgtgagcc accaagtggt tgctgggaat tgaactcaga acctctggaa
    12541 gagcagtcag tgctcttaac ctctgagcca tctctccagc ccctaaaaat ggctcttgag
    12601 atagggtctc aagtagtttg agactgagtt ggctatataa acaaggctgg cacatagcac
    12661 catgtacagc tgggtttagt ttacatgggg tgtttttgtc tctggaggca ggaggatcat
    12721 ttgagcatag ggagttaata gtgaggtcat gttttatcta ctcttctgaa ttgagaacta
    12781 agctgatgca aagcaagttt gactgaagaa gtccagttta tgagaacaag ggtggaaact
    12841 aatgtgtcaa agatggcctt gcatgtgttt tagatgatga cccagtcact tgggaattac
    12901 tggatgtgta agacctatat cttgacagga gtgaacagtg tcttataggt cctatatgaa
    12961 agaaatgaga catacccatt ttgtttcccc taagaattca cttttcctaa cctggttcat
    13021 gctatttagg ttattttact tgcaaatcct agggtgctcc cttacccagt attgcttatg
    13081 tggcaccaaa gtcactcact cccatgattt gcaagtctct gggaacttcc atgacaacct
    13141 agaatagcaa ctcaaataca ttttctcagt accaattttg aagaaaaaat attttgcaaa
    13201 atagctgtat ggatgggtac taaatagtga ggttatctcc agaaggccta tgaagaatta
    13261 aggttgagtt cagttgagtt cagcagcaag tttaaggttc atccattttt gtacagtgtt
    13321 ttcctattac ggtaagtgtt ttgcctgcag gaatatctgt accacatgct tgcctggtac
    13381 ctatatcggc cagaagaggg ctttggatcc tctggacttg aattacagat gggtattagc
    13441 caccatttag gtgctgggaa ttgaaaccaa gtcctctgga agaacagcaa gtgactcgac
    13501 gtccggagct ctggcgcgcc tgtggaat
//
