LOCUS       sequence-339096-       12570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CHO cells expression studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Orlova NA, Kovnir SV, Hodak JA, Vorobiev II, Gabibov AG, Skryabin KG
  TITLE     Improved elongation factor-1 alpha-based vectors for stable 
            high-level expression of heterologous proteins in Chinese hamster 
            ovary cells.
  JOURNAL   BMC Biotechnol. 2014 Jun 14;14:56. doi: 10.1186/1472-6750-14-56.
  PUBMED    24929670
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2014-06-14"; volume: "14:56. doi"; pages: " 
            10.1186/1472-6750-14-56"
COMMENT     Sequence Label: p1.2-Zeo-eGFP
FEATURES             Location/Qualifiers
     source          1..12570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     243..262
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        378..425
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             444..818
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    948..1069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(985..1004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1039..1058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1120..1137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1291..1879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1371..1390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2050..2841,2842..2910))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2673..2692
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2911..3015)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      7553..7562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7559..7561,7562..7564,7565..8278)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7559..7561,7562..7564,7565..8275)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7604..7625)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7865..7884)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8212..8233
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(join(12566..12570,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
      361 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
      421 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
      481 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
      541 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
      601 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
      661 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
      721 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
      781 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
      841 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
      901 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
      961 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     1021 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     1081 taccgtcgac ctctaggcgc gccatggctc ttccgcttcc tcgctcactg actcgctgcg
     1141 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1201 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1261 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1321 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1381 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1441 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1501 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1561 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1621 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1681 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1741 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1801 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     1981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2161 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     2761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     2821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     2881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     2941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3001 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcatctcatc acggtcacgc
     3061 atggctgcgc ccgcgcagcg ccccccgacc cccccccgat ccccggaacg ccagccgcca
     3121 tccaccgccc gcagcccccg gagcccgcgg accccgaccc cccgccgctc gcccgccgtt
     3181 gcgccgctct ctgcgggggg gctagggggc cgcgggggga aggccacgcc ccctccactt
     3241 tttccaggaa tgcgcggcat gacccgcccc ccccatgacc cgccaacacc cccccgatgc
     3301 cccccggggg tctttcctgg ggggcctttg tcagggttgc ctgtgtcacc cacgggcccc
     3361 ttggccacgc ttgcctgggt caccccggag ccccgggtca cgtttgccgg ggtcagccct
     3421 ggggcgcttc ttcacgctcc attgccatgc agaattcact agtgattaga atcccacgtt
     3481 gtgcatagaa acagatgcag gcaaaacatc cacacatata aaacaaaaaa ttaaaaccaa
     3541 taaaactcct aaacttttgg tctttcttga atcttcaatc cctcaggtta tgaaataatc
     3601 atttatgcag tcaaaaattt gccattcttg ttgccaggtg tggtgatgat tcggggaagc
     3661 agaagcaggc agatctctgt gaatgaggcc agcctggtct acaaagtgag tcccaggaca
     3721 gtcaggcctg ttacacagag aaaccttgaa aaaaaaaaga taatatgtac tgttgtatta
     3781 ccccaatata taaggctaaa ccattagaag cacaacactg ttaagtacgg aaaataatat
     3841 ctagtgtggt acagttacta ctactataat acactaatat agctgtggga aactagttcc
     3901 aaagatgaat tactaaccag tgtttccaag gaaataaatg aaagcagaga gattagttct
     3961 attgctagtg tttcattttc gtatatttct tacaatttct cttgttacaa ataggcacta
     4021 gggtatcaag ataattttaa cgactggctg agaaccctag aaaatctctg tgaaaaaggg
     4081 atttgtgaaa tgagagaggg taatgtggcc attatagaaa aggcttttgt gtgccttgca
     4141 tgcatagacc ctgtgtttga tctcttaaca ccctccttga ccagaaaaag cttctgtgga
     4201 tagaaaatga ttagttatat atacttttag ggaaacgtag ttctggattc tttggttaca
     4261 attaacagaa ttaagtgcaa acaaagccag aaacctcctg ataaatgaga aaacctgctt
     4321 gtagaaggtt gtaaggctct gtaatatagg aattaggaga aaagaaacct gtgtggtggg
     4381 gcacgtctgt aatcccagca ttgggaagta gaggtagaag attagaaatc aaaggccagc
     4441 ctcagcaaca cagtgagttt gaggccaccc tgaactacat caggttctgt ctcctttctt
     4501 tttttttttt ttcttttctt tttttggttt ctctgtgtag ttttggagcc tatcctggca
     4561 ctagctctga agagcaggct ggcctcgaac tcagagatca gccagcctct gctgggatta
     4621 aaggtatgca ccaccaacgc cccaggtttt gtctcaaaca aacaaaaata acatcaggag
     4681 gtggtgagag ggctcagtgg tcacaggcat tctctgcaaa gcctgactct gagttggatc
     4741 ctttagagct acatggttga gggaagagaa ctgactcctg gaaggtgtcc tctggtcccc
     4801 acacatagct atacacagca tgtgcattca cacacactaa ataatgctat ttttaaaaaa
     4861 attaaaaaca acaacagttt gggttgtgaa aactagaact agataatagg taagaatcaa
     4921 gtatcatgta aatttgcttt caactcatcc caaaatttgt tttatatttc agtttttttc
     4981 cttcctagct tgactgtgga gtcttgtccg gaagcaaata gttcctttgc agatcccaca
     5041 tgtggacacc ggacagtagg tcctcaaatg ctccttatta ggttggttca ataatatcaa
     5101 ttgtttgtta ctaggcagtg atgttgtaca tctggaggag atctcttgag cccataatca
     5161 ggttattagg aataaatact ctaaggctaa aaatgtagct tagtgataag agtgcttgcc
     5221 tggtgtgctg agaccctcgg ttccatctcc acaaccccat attccattac aaaatacctt
     5281 ttcaccgtcc ctagcattaa gaaacaaaac aacaaagaag tttttctttc ttctgagatc
     5341 ctgcccggag aggcatttaa aactggccag ggccaaaaaa aaaaaaaaaa gaaaaaaaag
     5401 aaaagaaaac aggctagggc cggcatggtg gcgcacgcct ttaatcccag cacgcaggag
     5461 gcagaggcag ggcggatctc tgtgagtttg aggtcagcct ggtctaccta gtgagtttca
     5521 gggcacccag ggctaaagag actgtctcaa aaacaaaaca gccacacaat cagaaccaca
     5581 gcaaaacgca gttatgatcc ttggaactgt aggaatgaca agcatttaaa taataggacg
     5641 agccattttt gagaagctct gatttcacaa gtgtcaggga tgggctctgg gcgagtaaga
     5701 ttgctaatgc tggcctctaa atgagaccac gtggagttga ttagattctt ttcatgttcc
     5761 tcgtgctcta tcaaataact gtacccaaat acacacacac acacacacac acaatgcgcg
     5821 cacacacaaa atcctttttt agcttaagaa gcccagaatc agaagtaaag ctaactgtgg
     5881 gacttaagta ttattctgaa cggaactccc agggcgtgaa gcgcgcttca ggcttccaga
     5941 gaagcagctg gcgctggatg gaatgaacca agaggccagc acaggggcag atccgtcgag
     6001 ctctcggcca ccgagctgag cccttaggtt ctggggctgg gaagggtccc taggattgtg
     6061 cacctctccc gcgggggaca agcaggggga tggcggggct gacgtcggga ggtggcctcc
     6121 acgggaaggg acacccggat ctcgacacag ccttggcagt ggagtcagga agggtaggga
     6181 cagattctgg acgccctctt ggccagctcc tcaccgcccc acccccgact ggagccgaga
     6241 gtaattcata caaaaggagg gatcgccttc gcccctggga atcccaggga ccgtcgctaa
     6301 attctggccg gcctcccagc ccggaaccgc tgtgcccgcc cagcgcggcg ggaggagcct
     6361 gcgcctaggg cggatcgcgg gtcggcggga gagcacaagc ccacagtccc cggcggtggg
     6421 ggaggggcgc gctgagcggg ggcccgggag ccagcgcggg gcaaactggg aaagtggcgt
     6481 cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa cggtataaaa gtgcggtagt
     6541 cgcgttggac gttctttttc gcaacgggtt tgccgtcaga acgcaggtga gtggcgggtg
     6601 tggcctccgc gggcccgggc tccctccttt gagcggggtc gggccgccgt gcgggtgtcg
     6661 tcggccgggc ttctctgcga gcgttcccgc cctggatggc gggctgtgcg ggagggcgag
     6721 ggggggaggc ctggcggcgg ccccggagcc tcgcctcgtg tcgggcgtga ggcctagcgt
     6781 ggcttccgcc ccgccgcgtg ccaccgcggc cgcgctttgc tgtctgcccg gctgccctcg
     6841 attgcctgcc cgcggcccgg gccaacaaag ggagggcgtg gagctggctg gtagggagcc
     6901 ccgtagtccg catgtcgggc agggagagcg gcagcagtcg ggggggggac cgggcccgcc
     6961 cgtcccgcag cacatgtcca acgccgcctg gacgggtagc ggcctgtgtc ctgataaggc
     7021 ggccgggcgg tgggttttag atgccgggtt caggtggccc cgggtcccgg cccggtctgg
     7081 ccagtacccc gtagtggctt agctccgagg agggcgaggc ccgcccgccc ggcaccagtt
     7141 gcgtgcgcgg aaagatggcc gctcccgggc cctgtagcaa ggagctcaaa atggaggacg
     7201 cggcagcccg gcggagcggg gcgggtgagt cacccacaca aaggaagagg gccttgcccc
     7261 tcgccggccg ctgcttcctg tgaccccgtg gtgtaccggc cgcacttcag tcaccccggg
     7321 cgctctttcg gagcaccgct ggcctccgct gggggagggg atctgtctaa tggcgttgga
     7381 gtttgctcac atttggtggg tggagactgt agccaggcca gcctggccat gaaagtaatt
     7441 cttggaattt gcccattttg agtttggagc gaagctgatt gacaaagctg cttagccgtt
     7501 caaaggtatt cttcgaactt tttttttaag gtgttgtgaa aacctcgagg ccgccaccat
     7561 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     7621 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     7681 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     7741 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     7801 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     7861 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     7921 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     7981 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     8041 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     8101 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     8161 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     8221 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagc
     8281 tagcatatta cccctaacac ctgccacccc agtcttaatc agtggtggaa gaacggtctc
     8341 agaactgttt gtctcaattg gccatttaag tttaatagtg aaagactggt taatgataac
     8401 aatgcatcgg aaaaccttca ggaggaaagg agaatgtttt gtggaacatt tttgtgtgtg
     8461 tggcagtttt aagttattag ttttcaaaat cagtactttt taatggaaac aacttgacca
     8521 aaaatctgtc acagaatttt gagacccatt aaaatacaag tttaatgaga agtctgtctc
     8581 tgttaatgct gaagtcatta ctaagtgctt agcttagcaa ggtatgtgga tgcccatttg
     8641 tgttccaagg gattggactg ttcatcagga cccagagctg agtttcaagg gctcaagaga
     8701 tggcttatta cctgtgggtg tcttgaaggt tctggttggg acaaattagg aatgtttttg
     8761 gcagacatgg tgactaccct tcatctgggt gagttcagtt gatttgtctt gagcctttgg
     8821 ggtttacaca agtaaatgac atcatacagt tagtgtattg ttagtgaata ttaatatatg
     8881 aggcaggctt tgctctagca attttagaac tagttttcag gaaagggttc atcttgtgca
     8941 ttggatgtct gattctatca cttagagttt aaactgaaag tgctcaagag gttttattta
     9001 ggctgggata taaataagcc tttctgtagc ttgtaatggt atcaggaatt taaaaggcca
     9061 tctggggcac aaagattaag cagaaaaggt agaaggtgag attgggggac tttgagtact
     9121 tcacacactt taatgtgtgg gtgctttagt gcatatagta caactgccag ataagggcat
     9181 ccacatctga ttgtttggaa ggcaccttgt ggtttctggg aattcagaat tgggagaaaa
     9241 agtgctccca accgctgaag cccttggtaa tctgcagggt gtttatttag caggagataa
     9301 ggacaaaaag ttatagtgtg gagttggttg agttggtaga tgtcattaca acaggtggtc
     9361 ttaaattggg ttaggagtca ctttgaaata cctgggccat aagcaaagtg gcattttcac
     9421 ctttcaggag aaactggtac acttatccat tctatagtgc atgcttgttc aattgggctg
     9481 atgactaaac cggtgactaa aggtttgtca gtataaatgg atgggttgta ggcagacggt
     9541 gaggaattac tatacctgca aggagtcatt gcctgatctg cctggaaagg ggcaggattg
     9601 agtctcagaa ggtgtacacc ataggatatg gaaaaatttg tcacgcctag cattcaactt
     9661 agtggtgtag cgccacctac tggcacttta aaagcttagc atagaggagc atgtgtgtta
     9721 ggagctcgga tgggatccag ggcctcaagg tttgcatgta aataaaagcc ctttaccaaa
     9781 ttaactacat accagcatac atcagtcctt tagtgttgaa aaacagaagt gaaagctaat
     9841 atatatagtg cttgctttat ttaagtctag ctgattacgt gtttggttgc cagtgtgact
     9901 agtctggagt tgaatttgtc ctcagacacg taaaatggaa tttgggattc acaacactct
     9961 agtatgaggg acctaatggc ctgtaccagg cacaaacgtg tctataaact acacaaaacg
    10021 aaggaattta caggaattag gaaggtattc ttaacattaa aacattatgg gcattttaaa
    10081 aaaagctttg acaggatttc tttgtcatgg ctgtcctgga gctagttgtg tagaccaggc
    10141 tgggctgaaa tcttgtctgc ctgcctggct tggacacttt tttattatgt atacaacatt
    10201 ctgcttccat gtatatctgc acattagaag acggcaccag atctcctaat ggatggttgt
    10261 gagccaccat gtggttgctg ggaattgaac tcaggacctc tggaagagca gtgctcttaa
    10321 cctctgagcc atctccagcc ccagcttggg cacattttta atggctggga aatcaaaccc
    10381 cctaggcctt ctgtcagtaa tgaagggctt ttggctaccg agagtaggat ttaaggttat
    10441 tcggagctgc aggtctgcct cagtgcaggt ttgggagtcc agcatcttag aaaatgcagt
    10501 gaagccaagc tgagctatat tttgtttaaa aaaaaaataa gtgggtaaag tgctgctgag
    10561 cctgatgacc aagctgggac acaagtagaa gaacataggc caatgctcta tattaaaagc
    10621 atgggtcatt tttaatgctc ttgagaaggc tatgcctaca ctactctcag ccaccgcagc
    10681 gtgtttaaat taaactagtt tggaaatttt ctttgggggt aagctattta acctagtgcc
    10741 ttggcaggta tactactgaa ctctcctcct cattcctttt tgttttttaa gaatttcagt
    10801 caggctcagg cagcccttaa acttgtgatt aagcctgaga acagttacga ttatgagcct
    10861 attagtatac cgatcaatat gtgaattttt ttgggatggg ggtcaggcct ccctgcctcc
    10921 caaatactgg gactaaaggc tgcaccacca caacctggct cttgaaatac ttttctacat
    10981 tttttggggg gcatgggtgg gagagcaggg tttctctgta ttagccctgg ctctcctgga
    11041 actctgtaga ccaggctatt cttgagctca gattagcctg tctctgcctc ctaaattctg
    11101 ggattaaagg tgtgtgctac tgctgcctgg ctacaaagac attttttttt ttcttaaatt
    11161 taaaaacaaa agtggttctt ttagaagggt ggttggtgtt ggcacatact ccaagcactc
    11221 aggttttgag tttgtcccag gaatgaagac tgcattactg ccgcccctcc ctggtaaggg
    11281 ctacacagag aaatcctatt tggagcctgt cctggtaact cgctctgtag accaggctgg
    11341 cctcgaactc aagagaacca cctgcctctg aatgctggta ttaagggcag gcaccaccaa
    11401 cacccagcct aaaaaatgtc ttttttttaa agattttttt ttttttttta cagaataaac
    11461 attctgttta caatattctg cttctatgta tatctgcaca ctagaagagg gcacccgatc
    11521 tcataatgga tggttgtgag ccaccaagtg gttgctggga attgaactca gaacctctgg
    11581 aagagcagtc agtgctctta acctctgagc catctctcca gcccctaaaa atggctcttg
    11641 agatagggtc tcaagtagtt tgagactgag ttggctatat aaacaaggct ggcacatagc
    11701 accatgtaca gctgggttta gtttacatgg ggtgtttttg tctctggagg caggaggatc
    11761 atttgagcat agggagttaa tagtgaggtc atgttttatc tactcttctg aattgagaac
    11821 taagctgatg caaagcaagt ttgactgaag aagtccagtt tatgagaaca agggtggaaa
    11881 ctaatgtgtc aaagatggcc ttgcatgtgt tttagatgat gacccagtca cttgggaatt
    11941 actggatgtg taagacctat atcttgacag gagtgaacag tgtcttatag gtcctatatg
    12001 aaagaaatga gacataccca ttttgtttcc cctaagaatt cacttttcct aacctggttc
    12061 atgctattta ggttatttta cttgcaaatc ctagggtgct cccttaccca gtattgctta
    12121 tgtggcacca aagtcactca ctcccatgat ttgcaagtct ctgggaactt ccatgacaac
    12181 ctagaatagc aactcaaata cattttctca gtaccaattt tgaagaaaaa atattttgca
    12241 aaatagctgt atggatgggt actaaatagt gaggttatct ccagaaggcc tatgaagaat
    12301 taaggttgag ttcagttgag ttcagcagca agtttaaggt tcatccattt ttgtacagtg
    12361 ttttcctatt acggtaagtg ttttgcctgc aggaatatct gtaccacatg cttgcctggt
    12421 acctatatcg gccagaagag ggctttggat cctctggact tgaattacag atgggtatta
    12481 gccaccattt aggtgctggg aattgaaacc aagtcctctg gaagaacagc aagtgactcg
    12541 acgtccggag ctctggcgcg cctgtggaat
//
