LOCUS       sequence-339101-        8864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cell cycle-dependent OsTIR1 fused with mEmerald and 
            Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 
            safe-harbor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Capece M, Tessari A, Mills J, Vinciguerra GLR, Louke D, Lin C, 
            McElwain BK, Miles WO, Coppola V, Davies AE, Palmieri D, Croce CM
  TITLE     A novel auxin-inducible degron system for rapid, cell cycle-specific
            targeted proteolysis.
  JOURNAL   Cell Death Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4.
  PUBMED    37537305
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2023 Aug 3. doi: 10.1038/s41418-023-01191-4."
COMMENT     Sequence Label: pDP1.2
FEATURES             Location/Qualifiers
     source          1..8864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..804
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     enhancer        861..1164
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1165..1368
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1319..1339
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1406..3130
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             join(3158..3160,3161..3163,3164..3874)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3203..3224)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3464..3483)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3811..3832
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3911..4240
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     polyA_signal    4417..4538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4454..4473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4508..4527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4547..5047
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4966..4984
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5069..5668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5069..5088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5565..5585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5675..5899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5902..6738
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(6772..6791)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6773..6791)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6798..6815)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6798..6814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(join(6817..7608,7609..7677))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7440..7459
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7801..8389
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8290..8309
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8543..8560
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8677..8698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8713..8718,8719..8736,8737..8743)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8751..8767
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8756..8778
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8775..8791
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8775..8791
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8812..8830
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgctttctct gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt
       61 ggcctgggtc acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt
      121 cttcctccac tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc
      181 cggagcactt ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg
      241 tctgggtcct ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct
      301 gggttccctt ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct
      361 tctccgacgg atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc
      421 gtttttctgg acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc
      481 ttgctttctt tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt
      541 catttgggca gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc
      601 ctgtcatggc atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc
      661 ccctatgtcc acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct
      721 gggaccacct tatattccca gggccggtta atgtggctct ggttctgggt acttttatct
      781 gtcccctcca ccccacagtg gggcaattaa tagtaatcaa ttacggggtc attagttcat
      841 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      901 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      961 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1021 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     1081 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1141 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     1201 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1261 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     1321 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     1381 cgtcagatcc gctagcgctg ccaccatgac atactttcct gaagaggtcg tcgaacacat
     1441 ttttagcttc ctgcctgcac agagagatag aaacacagtg agcctggtct gcaaagtgtg
     1501 gtacgagatc gaacgcctga gccggagagg agtgttcgtc ggcaactgct atgctgtgag
     1561 agcaggcagg gtcgccgcta ggtttccaaa tgtgcgcgca ctgaccgtca aggggaaacc
     1621 ccacttcgcc gactttaacc tggtgccccc tgattgggga ggatacgccg gcccttggat
     1681 cgaggcagcc gctcgcggct gtcatggact ggaggaactg cgcatgaagc gaatggtggt
     1741 ctctgacgaa agtctggagc tgctggctcg gagcttccct aggtttcgcg cactggtgct
     1801 gatttcttgc gaaggcttca gcaccgatgg actggcagcc gtggcctccc actgtaagct
     1861 gctgcgggag ctggacctcc aggagaatga agtggaggat agaggcccca gatggctgtc
     1921 ttgcttccca gactcatgta ccagcctggt gtccctgaac tttgcctgca tcaaaggcga
     1981 agtgaatgct gggtccctgg agcggctggt ctcaagaagc cccaacctga ggtctctgcg
     2041 gctgaaccgg agcgtgagcg tggacactct ggctaagatt ctgctgagaa cccctaacct
     2101 ggaggatctg ggaaccggca atctgacaga cgatttccag acagaatcct actttaaact
     2161 gacttctgcc ctggagaagt gtaaaatgct gaggagtctg tcaggattct gggatgcttc
     2221 acccgtgtgc ctgagcttta tctaccctct gtgtgcacag ctgacaggcc tgaacctgag
     2281 ctatgcacca accctggacg ccagtgatct gacaaagatg atctcacgct gcgtgaaact
     2341 ccagcgactg tgggtgctgg actgtatttc cgataagggg ctccaggtgg tcgccagctc
     2401 ctgcaaggac ctccaggagc tgagagtgtt cccatctgat ttttacgtgg ccggatatag
     2461 tgctgtcact gaggaaggcc tggtggcagt ctcactggga tgcccaaagc tgaacagcct
     2521 gctgtatttc tgtcatcaga tgactaatgc tgcactggtg accgtcgcca agaactgccc
     2581 taatttcacc cgatttcggc tgtgtattct ggaaccaggc aaacccgacg tggtcacatc
     2641 ccagccactg gatgaagggt ttggagctat cgtgagagag tgcaagggac tccagaggct
     2701 gagcatttcc ggcctgctga cagacaaagt gttcatgtac atcggcaagt atgctaagca
     2761 gctggagatg ctgagcattg catttgccgg agactccgat aagggcatga tgcacgtgat
     2821 gaacgggtgt aagaatctgc gaaaactgga aatccgggac agccctttcg gggatgccgc
     2881 tctgctggga aactttgcca gatacgagac aatgaggagc ctgtggatgt ctagttgcaa
     2941 tgtgactctg aagggctgtc aggtcctggc tagtaaaatg cctatgctga acgtggaagt
     3001 cattaatgag cgggacgggt ctaacgaaat ggaggaaaat catggcgacc tgccaaaggt
     3061 ggagaaactg tatgtgtatc ggaccaccgc aggggcaaga gatgatgctc ccaactttgt
     3121 gaagattctg ggaggttctg gtggttctgg tggttccatg gtgagcaagg gcgaggagct
     3181 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3241 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     3301 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tgacctacgg
     3361 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3421 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     3481 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3541 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     3601 ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga acttcaagac
     3661 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     3721 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     3781 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3841 cgggatcact ctcggcatgg acgagctgta caagcctagg ggatatccat cacactggcg
     3901 gccgctcgag atgaatccca gtatgaagca gaaacaagaa gaaatcaaag agaatataaa
     3961 gaatagttct gtcccaagaa gaactctgaa gatgattcag ccttctgcat ctggatctct
     4021 tgttggaaga gaaaatgagc tgtccgcagg cttgtccaaa aggaaacatc ggaatgacca
     4081 cttaacatct acaacttcca gccctggggt tattgtccca gaatctagtg aaaataaaaa
     4141 tcttggagga gtcacccagg agtcatttga tcttatgatt aaagaaaatc catcctctca
     4201 gtattggaag gaagtggcag aaaaacggag aaaggcgctg tgaggatcca tcggatcccg
     4261 ggcccgtcga cggtaccgcg ggcccgggat ccaccggggc cgcgactcta gatcataatc
     4321 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     4381 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     4441 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4501 tctagttgtg gtttgtccaa actcatcaat gtatcttaag ttggggttgc gccttttcca
     4561 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     4621 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     4681 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     4741 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     4801 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     4861 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     4921 tgcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     4981 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     5041 tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     5101 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     5161 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     5221 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     5281 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     5341 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     5401 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5461 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5521 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5581 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5641 gtgcatgacc cgcaagcccg gtgcctgagg taccctgtgc cttctagttg ccagccatct
     5701 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     5761 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     5821 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     5881 gatgcggtgg gctctatggt tactagggac aggattggtg acagaaaagc cccatcctta
     5941 ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg ttaggcagat
     6001 tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc cagaacctct
     6061 aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg cagggggtgg
     6121 gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc gctaccctct
     6181 cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc
     6241 agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca gaataagttg
     6301 gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat tgttcctccg
     6361 tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc agggctgtgg
     6421 tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt
     6481 caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt ccttaaagag
     6541 tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct tccctaaggc
     6601 cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc
     6661 ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct tccctacagg
     6721 ggttcctggc tctgctctaa gggcgaattc gcggccgcta aattcaattc gccctatagt
     6781 gagtcgtatt acaattcact ggccgtcgtt ttacaattac caatgcttaa tcagtgaggc
     6841 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6901 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6961 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7021 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7081 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7141 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7201 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7261 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7321 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7381 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7441 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7501 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7561 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7621 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7681 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga ccaaaatccc
     7741 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7801 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7861 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7921 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7981 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8041 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8101 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8161 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8221 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8281 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8341 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8401 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8461 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8521 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8581 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8641 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8701 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8761 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     8821 tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctt
//
