LOCUS       sequence-339102-       12713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for CHO expression studies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, 
            Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II
  TITLE     Approaches to Controlled Co-Amplification of Genes for Production of
            Biopharmaceuticals: Study of the Insertion and Amplification 
            Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary
            Cells during Co-Expression of Compatible Pair of Plasmids.
  JOURNAL   Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 
            10.1007/s10517-017-3776-0. Epub 2017 Jul 18.
  PUBMED    28726207
REFERENCE   2  (bases 1 to 12713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10517-017-3776-0"; journalName: "Bull Exp Biol Med"; date:
            "2017-06"; volume: "163"; issue: "2"; pages: "245-249"
COMMENT     Sequence Label: p1.1-mCherry
FEATURES             Location/Qualifiers
     source          1..12713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..27)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(181..769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(261..280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(940..1731,1732..1800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1563..1582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1801..1905)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      6443..6452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6449..7156
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6594..6612)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6862..6881
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7157..7222
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    7263..7836
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7286..7838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7430..7447)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7657..7676
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7853..7862
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7859..8422
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
ORIGIN
        1 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
       61 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      601 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1921 agtgccacct gcatctcatc acggtcacgc atggctgcgc ccgcgcagcg ccccccgacc
     1981 cccccccgat ccccggaacg ccagccgcca tccaccgccc gcagcccccg gagcccgcgg
     2041 accccgaccc cccgccgctc gcccgccgtt gcgccgctct ctgcgggggg gctagggggc
     2101 cgcgggggga aggccacgcc ccctccactt tttccaggaa tgcgcggcat gacccgcccc
     2161 ccccatgacc cgccaacacc cccccgatgc cccccggggg tctttcctgg ggggcctttg
     2221 tcagggttgc ctgtgtcacc cacgggcccc ttggccacgc ttgcctgggt caccccggag
     2281 ccccgggtca cgtttgccgg ggtcagccct ggggcgcttc ttcacgctcc attgccatgc
     2341 agaattcact agtgattaga atcccacgtt gtgcatagaa acagatgcag gcaaaacatc
     2401 cacacatata aaacaaaaaa ttaaaaccaa taaaactcct aaacttttgg tctttcttga
     2461 atcttcaatc cctcaggtta tgaaataatc atttatgcag tcaaaaattt gccattcttg
     2521 ttgccaggtg tggtgatgat tcggggaagc agaagcaggc agatctctgt gaatgaggcc
     2581 agcctggtct acaaagtgag tcccaggaca gtcaggcctg ttacacagag aaaccttgaa
     2641 aaaaaaaaga taatatgtac tgttgtatta ccccaatata taaggctaaa ccattagaag
     2701 cacaacactg ttaagtacgg aaaataatat ctagtgtggt acagttacta ctactataat
     2761 acactaatat agctgtggga aactagttcc aaagatgaat tactaaccag tgtttccaag
     2821 gaaataaatg aaagcagaga gattagttct attgctagtg tttcattttc gtatatttct
     2881 tacaatttct cttgttacaa ataggcacta gggtatcaag ataattttaa cgactggctg
     2941 agaaccctag aaaatctctg tgaaaaaggg atttgtgaaa tgagagaggg taatgtggcc
     3001 attatagaaa aggcttttgt gtgccttgca tgcatagacc ctgtgtttga tctcttaaca
     3061 ccctccttga ccagaaaaag cttctgtgga tagaaaatga ttagttatat atacttttag
     3121 ggaaacgtag ttctggattc tttggttaca attaacagaa ttaagtgcaa acaaagccag
     3181 aaacctcctg ataaatgaga aaacctgctt gtagaaggtt gtaaggctct gtaatatagg
     3241 aattaggaga aaagaaacct gtgtggtggg gcacgtctgt aatcccagca ttgggaagta
     3301 gaggtagaag attagaaatc aaaggccagc ctcagcaaca cagtgagttt gaggccaccc
     3361 tgaactacat caggttctgt ctcctttctt tttttttttt ttcttttctt tttttggttt
     3421 ctctgtgtag ttttggagcc tatcctggca ctagctctga agagcaggct ggcctcgaac
     3481 tcagagatca gccagcctct gctgggatta aaggtatgca ccaccaacgc cccaggtttt
     3541 gtctcaaaca aacaaaaata acatcaggag gtggtgagag ggctcagtgg tcacaggcat
     3601 tctctgcaaa gcctgactct gagttggatc ctttagagct acatggttga gggaagagaa
     3661 ctgactcctg gaaggtgtcc tctggtcccc acacatagct atacacagca tgtgcattca
     3721 cacacactaa ataatgctat ttttaaaaaa attaaaaaca acaacagttt gggttgtgaa
     3781 aactagaact agataatagg taagaatcaa gtatcatgta aatttgcttt caactcatcc
     3841 caaaatttgt tttatatttc agtttttttc cttcctagct tgactgtgga gtcttgtccg
     3901 gaagcaaata gttcctttgc agatcccaca tgtggacacc ggacagtagg tcctcaaatg
     3961 ctccttatta ggttggttca ataatatcaa ttgtttgtta ctaggcagtg atgttgtaca
     4021 tctggaggag atctcttgag cccataatca ggttattagg aataaatact ctaaggctaa
     4081 aaatgtagct tagtgataag agtgcttgcc tggtgtgctg agaccctcgg ttccatctcc
     4141 acaaccccat attccattac aaaatacctt ttcaccgtcc ctagcattaa gaaacaaaac
     4201 aacaaagaag tttttctttc ttctgagatc ctgcccggag aggcatttaa aactggccag
     4261 ggccaaaaaa aaaaaaaaaa gaaaaaaaag aaaagaaaac aggctagggc cggcatggtg
     4321 gcgcacgcct ttaatcccag cacgcaggag gcagaggcag ggcggatctc tgtgagtttg
     4381 aggtcagcct ggtctaccta gtgagtttca gggcacccag ggctaaagag actgtctcaa
     4441 aaacaaaaca gccacacaat cagaaccaca gcaaaacgca gttatgatcc ttggaactgt
     4501 aggaatgaca agcatttaaa taataggacg agccattttt gagaagctct gatttcacaa
     4561 gtgtcaggga tgggctctgg gcgagtaaga ttgctaatgc tggcctctaa atgagaccac
     4621 gtggagttga ttagattctt ttcatgttcc tcgtgctcta tcaaataact gtacccaaat
     4681 acacacacac acacacacac acaatgcgcg cacacacaaa atcctttttt agcttaagaa
     4741 gcccagaatc agaagtaaag ctaactgtgg gacttaagta ttattctgaa cggaactccc
     4801 agggcgtgaa gcgcgcttca ggcttccaga gaagcagctg gcgctggatg gaatgaacca
     4861 agaggccagc acaggggcag atccgtcgag ctctcggcca ccgagctgag cccttaggtt
     4921 ctggggctgg gaagggtccc taggattgtg cacctctccc gcgggggaca agcaggggga
     4981 tggcggggct gacgtcggga ggtggcctcc acgggaaggg acacccggat ctcgacacag
     5041 ccttggcagt ggagtcagga agggtaggga cagattctgg acgccctctt ggccagctcc
     5101 tcaccgcccc acccccgact ggagccgaga gtaattcata caaaaggagg gatcgccttc
     5161 gcccctggga atcccaggga ccgtcgctaa attctggccg gcctcccagc ccggaaccgc
     5221 tgtgcccgcc cagcgcggcg ggaggagcct gcgcctaggg cggatcgcgg gtcggcggga
     5281 gagcacaagc ccacagtccc cggcggtggg ggaggggcgc gctgagcggg ggcccgggag
     5341 ccagcgcggg gcaaactggg aaagtggcgt cgtgtgctgg ctccgccctc ttcccgaggg
     5401 tgggggagaa cggtataaaa gtgcggtagt cgcgttggac gttctttttc gcaacgggtt
     5461 tgccgtcaga acgcaggtga gtggcgggtg tggcctccgc gggcccgggc tccctccttt
     5521 gagcggggtc gggccgccgt gcgggtgtcg tcggccgggc ttctctgcga gcgttcccgc
     5581 cctggatggc gggctgtgcg ggagggcgag ggggggaggc ctggcggcgg ccccggagcc
     5641 tcgcctcgtg tcgggcgtga ggcctagcgt ggcttccgcc ccgccgcgtg ccaccgcggc
     5701 cgcgctttgc tgtctgcccg gctgccctcg attgcctgcc cgcggcccgg gccaacaaag
     5761 ggagggcgtg gagctggctg gtagggagcc ccgtagtccg catgtcgggc agggagagcg
     5821 gcagcagtcg ggggggggac cgggcccgcc cgtcccgcag cacatgtcca acgccgcctg
     5881 gacgggtagc ggcctgtgtc ctgataaggc ggccgggcgg tgggttttag atgccgggtt
     5941 caggtggccc cgggtcccgg cccggtctgg ccagtacccc gtagtggctt agctccgagg
     6001 agggcgaggc ccgcccgccc ggcaccagtt gcgtgcgcgg aaagatggcc gctcccgggc
     6061 cctgtagcaa ggagctcaaa atggaggacg cggcagcccg gcggagcggg gcgggtgagt
     6121 cacccacaca aaggaagagg gccttgcccc tcgccggccg ctgcttcctg tgaccccgtg
     6181 gtgtaccggc cgcacttcag tcaccccggg cgctctttcg gagcaccgct ggcctccgct
     6241 gggggagggg atctgtctaa tggcgttgga gtttgctcac atttggtggg tggagactgt
     6301 agccaggcca gcctggccat gaaagtaatt cttggaattt gcccattttg agtttggagc
     6361 gaagctgatt gacaaagctg cttagccgtt caaaggtatt cttcgaactt tttttttaag
     6421 gtgttgtgaa aacctcgagg ccgccaccat ggtgagcaag ggcgaggagg ataacatggc
     6481 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     6541 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     6601 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     6661 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     6721 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     6781 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     6841 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     6901 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     6961 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     7021 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     7081 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     7141 ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     7201 cggtaccgcg ggcccgggat ccaccggatc tagataagct agcggccgcg cagttaacgc
     7261 cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     7321 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     7381 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     7441 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     7501 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     7561 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     7621 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     7681 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     7741 tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     7801 ggggacgtgg ttttcctttg aaaaacacga tgataatatg gccacaagat ctgccaccat
     7861 ggttcgacca ttgaactgca tcgtcgccgt gtcccaaaat atggggattg gcaagaacgg
     7921 agacctaccc tggcctccgc tcaggaacga gttcaagtac ttccaaagaa tgaccacaac
     7981 ctcttcagtg gaaggtaaac agaatctggt gattatgggt aggaaaacct ggttctccat
     8041 tcctgagaag aatcgacctc taaaggacag aattaatata gttctcagta gagaactcaa
     8101 agaaccacca cgaggagctc attttcttgc caaaagtttg gatgatgcct taagacttat
     8161 tgaacaaccg gaattggcaa gtaaagtaga catggtttgg atagtcggag gcagttctgt
     8221 ttaccaggaa gccatgaatc aaccaggcca cctcagactc tttgtgacaa ggatcatgca
     8281 ggaatttgaa agtgacacgt ttttcccaga aattgatttg gggaaatata aacttctccc
     8341 agaataccca ggcgtcctct ctgaggtcca ggaggaaaaa ggcatcaagt ataagtttga
     8401 agtctacgag aagaaagact aatctagcat attaccccta acacctgcca ccccagtctt
     8461 aatcagtggt ggaagaacgg tctcagaact gtttgtctca attggccatt taagtttaat
     8521 agtgaaagac tggttaatga taacaatgca tcggaaaacc ttcaggagga aaggagaatg
     8581 ttttgtggaa catttttgtg tgtgtggcag ttttaagtta ttagttttca aaatcagtac
     8641 tttttaatgg aaacaacttg accaaaaatc tgtcacagaa ttttgagacc cattaaaata
     8701 caagtttaat gagaagtctg tctctgttaa tgctgaagtc attactaagt gcttagctta
     8761 gcaaggtatg tggatgccca tttgtgttcc aagggattgg actgttcatc aggacccaga
     8821 gctgagtttc aagggctcaa gagatggctt attacctgtg ggtgtcttga aggttctggt
     8881 tgggacaaat taggaatgtt tttggcagac atggtgacta cccttcatct gggtgagttc
     8941 agttgatttg tcttgagcct ttggggttta cacaagtaaa tgacatcata cagttagtgt
     9001 attgttagtg aatattaata tatgaggcag gctttgctct agcaatttta gaactagttt
     9061 tcaggaaagg gttcatcttg tgcattggat gtttgattct atcacttaga gtttaaactg
     9121 aaagtgctca agaggtttta tttaggctgg gatataaata agcctttctg tagcttgtaa
     9181 tggtatcagg aatttaaaag gccatctggg gcacaaagat taagcagaaa aggtagaagg
     9241 tgagattggg ggactttgag tacttcacac actttaatgt gtgagtgctt tagtgcatat
     9301 agtacaactg ccagataagg gcatccacat ctgattgttt ggaaggcacc ttgtggtttc
     9361 tgggaattca gaattgggag aaaaagtgct cccaaccgct gaagcccttg gtaatctgca
     9421 gggtgtttat ttagcaggag ataaggacaa aaagttatag tgtggagttg gttgagttgg
     9481 tagatgtcat tacaacaggt ggtcttaaat tgggttagga gtcactttga aatacctggg
     9541 ccataagcaa agtggcattt tcacctttca ggagaaactg gtacacttat ccattctata
     9601 gtgcatgctt gttcaattgg gctgatgact aaaccggtga ctaaaggttt gtcagtataa
     9661 atggatgggt tgtaggcaga cggtgaggaa ttactatacc tgcaaggagt cattgcctga
     9721 tctgcctgga aaggggcagg attgagtctc agaaggtgta caccatagga tatggaaaaa
     9781 tttgtcacgc ctagcattca acttagtggt gtagcgccac ctactggcac tttaaaagct
     9841 tagcatagag gagcatgtgt gttaggagct cggatgggat ccagggcctc aaggtttgca
     9901 tgtaaataaa agccctttac caaattaact acataccagc atacatcagt cctttagtgt
     9961 tgaaaaacag aagtgaaagc taatatatat agtgcttgct ttatttaagt ctagctgatt
    10021 acgtgtttgg ttgccagtgt gactagtctg gagttgaatt tgtcctcaga cacgtaaaat
    10081 ggaatttggg attcacaaca ctctagtatg agggacctaa tggcctgtac caggcacaaa
    10141 cgtgtctata aactacacaa aacgaaggaa tttacaggaa ttaggaaggt attcttaaca
    10201 ttaaaacatt atgggcattt taaaaaaagc tttgacagga tttctttgtc atggctgtcc
    10261 tggagctagt tgtgtagacc aggctgggct gaaatcttgt ctgcctgcct ggcttggaca
    10321 cttttttatt atgtatacaa cattctgctt ccatgtatat ctgcacatta gaagacggca
    10381 ccagatctcc taatggatgg ttgtgagcca ccatgtggtt gctgggaatt gaactcagga
    10441 cctctggaag agcagtgctc ttaacctctg agccatctcc agccccagct tgggcacatt
    10501 tttaatggct gggaaatcaa accccctagg ccttctgtca gtaatgaagg gcttttggct
    10561 accgagagta ggatttaagg ttattcggag ctgcaggtct gcctcagtgc aggtttggga
    10621 gtccagcatc ttagaaaatg cagtgaagcc aagctgagct atattttgtt taaaaaaaaa
    10681 ataagtgggt aaagtgctgc tgagcctgat gaccaagctg ggacacaagt agaagaacat
    10741 aggccaatgc tctatattaa aagcatgggt catttttaat gctcttgaga aggctatgcc
    10801 tacactactc tcagccaccg cagcgtgttt aaattaaact agtttggaaa ttttctttgg
    10861 gggtaagcta tttaacctag tgccttggca ggtatactac tgaactctcc tcctcattcc
    10921 tttttgtttt ttaagaattt cagtcaggct caggcagccc ttaaacttgt gattaagcct
    10981 gagaacagtt acgattatga gcctattagt ataccgatca atatgtgaat ttttttggga
    11041 tgggggtcag gcctccctgc ctcccaaata ctgggactaa aggctgcacc accacaacct
    11101 ggctcttgaa atacttttct acattttttg gggggcatgg gtgggagagc agggtttctc
    11161 tgtattagcc ctggctctcc tggaactctg tagaccaggc tattcttgag ctcagattag
    11221 cctgtctctg cctcctaaat tctgggatta aaggtgtgtg ctactgctgc ctggctacaa
    11281 agacattttt ttttttctta aatttaaaaa caaaagtggt tcttttagaa gggtggttgg
    11341 tgttggcaca tactccaagc actcaggttt tgagtttgtc ccaggaatga agactgcatt
    11401 actgccgccc ctccctggta agggctacac agagaaatcc tatttggagc ctgtcctggt
    11461 aactcgctct gtagaccagg ctggcctcga actcaagaga accacctgcc tctgaatgct
    11521 ggtattaagg gcaggcacca ccaacaccca gcctaaaaaa tgtctttttt ttaaagattt
    11581 tttttttttt tttacagaat aaacattctg tttacaatat tctgcttcta tgtatatctg
    11641 cacactagaa gagggcaccc gatctcataa tggatggttg tgagccacca agtggttgct
    11701 gggaattgaa ctcagaacct ctggaagagc agtcagtgct cttaacctct gagccatctc
    11761 tccagcccct aaaaatggct cttgagatag ggtctcaagt agtttgagac tgagttggct
    11821 atataaacaa ggctggcaca tagcaccatg tacagctggg tttagtttac atggggtgtt
    11881 tttgtctctg gaggcaggag gatcatttga gcatagggag ttaatagtga ggtcatgttt
    11941 tatctactct tctgaattga gaactaagct gatgcaaagc aagtttgact gaagaagtcc
    12001 agtttatgag aacaagggtg gaaactaatg tgtcaaagat ggccttgcat gtgttttaga
    12061 tgatgaccca gtcacttggg aattactgga tgtgtaagac ctatatcttg acaggagtga
    12121 acagtgtctt ataggtccta tatgaaagaa atgagacata cccattttgt ttcccctaag
    12181 aattcacttt tcctaacctg gttcatgcta tttaggttat tttacttgca aatcctaggg
    12241 tgctccctta cccagtattg cttatgtggc accaaagtca ctcactccca tgatttgcaa
    12301 gtctctggga acttccatga caacctagaa tagcaactca aatacatttt ctcagtacca
    12361 attttgaaga aaaaatattt tgcaaaatag ctgtatggat gggtactaaa tagtgaggtt
    12421 atctccagaa ggcctatgaa gaattaaggt tgagttcagt tgagttcagc agcaagttta
    12481 aggttcatcc atttttgtac agtgttttcc tattacggta agtgttttgc ctgcaggaat
    12541 atctgtacca catgcttgcc tggtacctat atcggccaga agagggcttt ggatcctctg
    12601 gacttgaatt acagatgggt attagccacc atttaggtgc tgggaattga aaccaagtcc
    12661 tctggaagaa cagcaagtga ctcgacgtcc ggagctctgg cgcgccatgg ctc
//
