LOCUS       sequence-339105-       12814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in CHO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12814)
  AUTHORS   Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, 
            Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II
  TITLE     Approaches to Controlled Co-Amplification of Genes for Production of
            Biopharmaceuticals: Study of the Insertion and Amplification 
            Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary
            Cells during Co-Expression of Compatible Pair of Plasmids.
  JOURNAL   Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 
            10.1007/s10517-017-3776-0. Epub 2017 Jul 18.
  PUBMED    28726207
REFERENCE   2  (bases 1 to 12814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s10517-017-3776-0"; journalName: "Bull Exp Biol Med"; date:
            "2017-06"; volume: "163"; issue: "2"; pages: "245-249"
COMMENT     Sequence Label: p1.2-GS
FEATURES             Location/Qualifiers
     source          1..12814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..330
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      181..316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1906..2027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1943..1962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1997..2016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2078..2095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2249..2837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2329..2348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3008..3799,3800..3868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3631..3650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3869..3973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(12810..12814,1..16))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
       61 acgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
      121 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
      181 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
      241 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
      301 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttggat atccattttc
      361 gcatcgatgc ttccaccatg gccacctcag caagttccca cttgaacaaa ggcatcaagc
      421 agatgtacat gaacctgccc cagggcgaga agatccaact catgtatatc tgggttgatg
      481 gtaccgggga agggctacgc tgcaagaccc gtactctgga ctgtgacccc aagtgtgtag
      541 aagagttacc cgagtggaac tttgatggtt ctagtacgtt tcagtctgaa ggctccaaca
      601 gcgacatgta cctccatcct gtggccatgt ttcgagaccc cttccgcaga gaccccaaca
      661 agctggtgtt ctgcgaagta ttcaagtata accggaagcc cgcagagacc aacctgaggc
      721 acagctgtaa gcgtataatg gacatggtga gcagccagca cccctggttt ggaatggaac
      781 aggagtatac tctcatggga acagacggcc accctttcgg ctggccttct aatggcttcc
      841 ctggacccca aggaccctat tactgcggtg tgggagctga caaggcttat ggccgagata
      901 tcgtggaggc tcactaccgg gcctgcttgt atgctggaat caagatcaca gggacaaatg
      961 ccgaggttat gcctgcccag tgggaattcc agataggacc ctgcgaaggg atccgcatgg
     1021 gagatcatct ctgggtagcc cgttttatct tgcatcgggt atgcgaagac tttggggtga
     1081 tagcaacctt tgaccccaag cccattccag ggaactggaa tggggcaggc tgccacacca
     1141 actttagcac caaggccatg cgggaggaga atggtctgag gtgcattgag gaggccattg
     1201 ataaactgag caagaggcac cagtaccaca tccgtgccta cgaccccaag gggggcctgg
     1261 acaacgcccg ccgtctgact ggattccacg aaacctccaa catcaacgac ttttccgctg
     1321 gcgttgccaa ccgcagcgcc agtatccgca ttccccggat tgtcggccag gagaagaagg
     1381 gttactttga agaccgtcgg ccttctgcca attgcgaccc ctatgcggtg acggaagcca
     1441 tcgtccgcac gtgtctcctc aacgaaactg gcgacgagcc cttccaatac aagaactaag
     1501 cggactcgac ttccagtgat cttgagccct tcctagttca ccccactccc aactgttccc
     1561 tctcccactg gtccccactg taactcaaaa ggatggaata ccaaggtctt tttattcctt
     1621 gcgcccagtt aatttttgcc tttattggtc agaatagagg ggtcaggttc ttaatctcta
     1681 cacacccaac cccttctttc ctagctatcg ccttcttgac gagttcttct gagcgggact
     1741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1861 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     1921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     2041 ccgtcgacct ctaggcgcgc catggctctt ccgcttcctc gctcactgac tcgctgcgct
     2101 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2161 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2221 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2281 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2341 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2401 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2461 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2521 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2581 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2641 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2701 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2761 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2821 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2881 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2941 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3001 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3061 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3961 taggggttcc gcgcacattt ccccgaaaag tgccacctgc atctcatcac ggtcacgcat
     4021 ggctgcgccc gcgcagcgcc ccccgacccc cccccgatcc ccggaacgcc agccgccatc
     4081 caccgcccgc agcccccgga gcccgcggac cccgaccccc cgccgctcgc ccgccgttgc
     4141 gccgctctct gcgggggggc tagggggccg cggggggaag gccacgcccc ctccactttt
     4201 tccaggaatg cgcggcatga cccgcccccc ccatgacccg ccaacacccc cccgatgccc
     4261 cccgggggtc tttcctgggg ggcctttgtc agggttgcct gtgtcaccca cgggcccctt
     4321 ggccacgctt gcctgggtca ccccggagcc ccgggtcacg tttgccgggg tcagccctgg
     4381 ggcgcttctt cacgctccat tgccatgcag aattcactag tgattagaat cccacgttgt
     4441 gcatagaaac agatgcaggc aaaacatcca cacatataaa acaaaaaatt aaaaccaata
     4501 aaactcctaa acttttggtc tttcttgaat cttcaatccc tcaggttatg aaataatcat
     4561 ttatgcagtc aaaaatttgc cattcttgtt gccaggtgtg gtgatgattc ggggaagcag
     4621 aagcaggcag atctctgtga atgaggccag cctggtctac aaagtgagtc ccaggacagt
     4681 caggcctgtt acacagagaa accttgaaaa aaaaaagata atatgtactg ttgtattacc
     4741 ccaatatata aggctaaacc attagaagca caacactgtt aagtacggaa aataatatct
     4801 agtgtggtac agttactact actataatac actaatatag ctgtgggaaa ctagttccaa
     4861 agatgaatta ctaaccagtg tttccaagga aataaatgaa agcagagaga ttagttctat
     4921 tgctagtgtt tcattttcgt atatttctta caatttctct tgttacaaat aggcactagg
     4981 gtatcaagat aattttaacg actggctgag aaccctagaa aatctctgtg aaaaagggat
     5041 ttgtgaaatg agagagggta atgtggccat tatagaaaag gcttttgtgt gccttgcatg
     5101 catagaccct gtgtttgatc tcttaacacc ctccttgacc agaaaaagct tctgtggata
     5161 gaaaatgatt agttatatat acttttaggg aaacgtagtt ctggattctt tggttacaat
     5221 taacagaatt aagtgcaaac aaagccagaa acctcctgat aaatgagaaa acctgcttgt
     5281 agaaggttgt aaggctctgt aatataggaa ttaggagaaa agaaacctgt gtggtggggc
     5341 acgtctgtaa tcccagcatt gggaagtaga ggtagaagat tagaaatcaa aggccagcct
     5401 cagcaacaca gtgagtttga ggccaccctg aactacatca ggttctgtct cctttctttt
     5461 tttttttttt cttttctttt tttggtttct ctgtgtagtt ttggagccta tcctggcact
     5521 agctctgaag agcaggctgg cctcgaactc agagatcagc cagcctctgc tgggattaaa
     5581 ggtatgcacc accaacgccc caggttttgt ctcaaacaaa caaaaataac atcaggaggt
     5641 ggtgagaggg ctcagtggtc acaggcattc tctgcaaagc ctgactctga gttggatcct
     5701 ttagagctac atggttgagg gaagagaact gactcctgga aggtgtcctc tggtccccac
     5761 acatagctat acacagcatg tgcattcaca cacactaaat aatgctattt ttaaaaaaat
     5821 taaaaacaac aacagtttgg gttgtgaaaa ctagaactag ataataggta agaatcaagt
     5881 atcatgtaaa tttgctttca actcatccca aaatttgttt tatatttcag tttttttcct
     5941 tcctagcttg actgtggagt cttgtccgga agcaaatagt tcctttgcag atcccacatg
     6001 tggacaccgg acagtaggtc ctcaaatgct ccttattagg ttggttcaat aatatcaatt
     6061 gtttgttact aggcagtgat gttgtacatc tggaggagat ctcttgagcc cataatcagg
     6121 ttattaggaa taaatactct aaggctaaaa atgtagctta gtgataagag tgcttgcctg
     6181 gtgtgctgag accctcggtt ccatctccac aaccccatat tccattacaa aatacctttt
     6241 caccgtccct agcattaaga aacaaaacaa caaagaagtt tttctttctt ctgagatcct
     6301 gcccggagag gcatttaaaa ctggccaggg ccaaaaaaaa aaaaaaaaga aaaaaaagaa
     6361 aagaaaacag gctagggccg gcatggtggc gcacgccttt aatcccagca cgcaggaggc
     6421 agaggcaggg cggatctctg tgagtttgag gtcagcctgg tctacctagt gagtttcagg
     6481 gcacccaggg ctaaagagac tgtctcaaaa acaaaacagc cacacaatca gaaccacagc
     6541 aaaacgcagt tatgatcctt ggaactgtag gaatgacaag catttaaata ataggacgag
     6601 ccatttttga gaagctctga tttcacaagt gtcagggatg ggctctgggc gagtaagatt
     6661 gctaatgctg gcctctaaat gagaccacgt ggagttgatt agattctttt catgttcctc
     6721 gtgctctatc aaataactgt acccaaatac acacacacac acacacacac aatgcgcgca
     6781 cacacaaaat ccttttttag cttaagaagc ccagaatcag aagtaaagct aactgtggga
     6841 cttaagtatt attctgaacg gaactcccag ggcgtgaagc gcgcttcagg cttccagaga
     6901 agcagctggc gctggatgga atgaaccaag aggccagcac aggggcagat ccgtcgagct
     6961 ctcggccacc gagctgagcc cttaggttct ggggctggga agggtcccta ggattgtgca
     7021 cctctcccgc gggggacaag cagggggatg gcggggctga cgtcgggagg tggcctccac
     7081 gggaagggac acccggatct cgacacagcc ttggcagtgg agtcaggaag ggtagggaca
     7141 gattctggac gccctcttgg ccagctcctc accgccccac ccccgactgg agccgagagt
     7201 aattcataca aaaggaggga tcgccttcgc ccctgggaat cccagggacc gtcgctaaat
     7261 tctggccggc ctcccagccc ggaaccgctg tgcccgccca gcgcggcggg aggagcctgc
     7321 gcctagggcg gatcgcgggt cggcgggaga gcacaagccc acagtccccg gcggtggggg
     7381 aggggcgcgc tgagcggggg cccgggagcc agcgcggggc aaactgggaa agtggcgtcg
     7441 tgtgctggct ccgccctctt cccgagggtg ggggagaacg gtataaaagt gcggtagtcg
     7501 cgttggacgt tctttttcgc aacgggtttg ccgtcagaac gcaggtgagt ggcgggtgtg
     7561 gcctccgcgg gcccgggctc cctcctttga gcggggtcgg gccgccgtgc gggtgtcgtc
     7621 ggccgggctt ctctgcgagc gttcccgccc tggatggcgg gctgtgcggg agggcgaggg
     7681 ggggaggcct ggcggcggcc ccggagcctc gcctcgtgtc gggcgtgagg cctagcgtgg
     7741 cttccgcccc gccgcgtgcc accgcggccg cgctttgctg tctgcccggc tgccctcgat
     7801 tgcctgcccg cggcccgggc caacaaaggg agggcgtgga gctggctggt agggagcccc
     7861 gtagtccgca tgtcgggcag ggagagcggc agcagtcggg ggggggaccg ggcccgcccg
     7921 tcccgcagca catgtccaac gccgcctgga cgggtagcgg cctgtgtcct gataaggcgg
     7981 ccgggcggtg ggttttagat gccgggttca ggtggccccg ggtcccggcc cggtctggcc
     8041 agtaccccgt agtggcttag ctccgaggag ggcgaggccc gcccgcccgg caccagttgc
     8101 gtgcgcggaa agatggccgc tcccgggccc tgtagcaagg agctcaaaat ggaggacgcg
     8161 gcagcccggc ggagcggggc gggtgagtca cccacacaaa ggaagagggc cttgcccctc
     8221 gccggccgct gcttcctgtg accccgtggt gtaccggccg cacttcagtc accccgggcg
     8281 ctctttcgga gcaccgctgg cctccgctgg gggaggggat ctgtctaatg gcgttggagt
     8341 ttgctcacat ttggtgggtg gagactgtag ccaggccagc ctggccatga aagtaattct
     8401 tggaatttgc ccattttgag tttggagcga agctgattga caaagctgct tagccgttca
     8461 aaggtattct tcgaactttt tttttaaggt gttgtgaaaa cctcgaggta cgtatcgcga
     8521 aagctagcat attaccccta acacctgcca ccccagtctt aatcagtggt ggaagaacgg
     8581 tctcagaact gtttgtctca attggccatt taagtttaat agtgaaagac tggttaatga
     8641 taacaatgca tcggaaaacc ttcaggagga aaggagaatg ttttgtggaa catttttgtg
     8701 tgtgtggcag ttttaagtta ttagttttca aaatcagtac tttttaatgg aaacaacttg
     8761 accaaaaatc tgtcacagaa ttttgagacc cattaaaata caagtttaat gagaagtctg
     8821 tctctgttaa tgctgaagtc attactaagt gcttagctta gcaaggtatg tggatgccca
     8881 tttgtgttcc aagggattgg actgttcatc aggacccaga gctgagtttc aagggctcaa
     8941 gagatggctt attacctgtg ggtgtcttga aggttctggt tgggacaaat taggaatgtt
     9001 tttggcagac atggtgacta cccttcatct gggtgagttc agttgatttg tcttgagcct
     9061 ttggggttta cacaagtaaa tgacatcata cagttagtgt attgttagtg aatattaata
     9121 tatgaggcag gctttgctct agcaatttta gaactagttt tcaggaaagg gttcatcttg
     9181 tgcattggat gtctgattct atcacttaga gtttaaactg aaagtgctca agaggtttta
     9241 tttaggctgg gatataaata agcctttctg tagcttgtaa tggtatcagg aatttaaaag
     9301 gccatctggg gcacaaagat taagcagaaa aggtagaagg tgagattggg ggactttgag
     9361 tacttcacac actttaatgt gtgggtgctt tagtgcatat agtacaactg ccagataagg
     9421 gcatccacat ctgattgttt ggaaggcacc ttgtggtttc tgggaattca gaattgggag
     9481 aaaaagtgct cccaaccgct gaagcccttg gtaatctgca gggtgtttat ttagcaggag
     9541 ataaggacaa aaagttatag tgtggagttg gttgagttgg tagatgtcat tacaacaggt
     9601 ggtcttaaat tgggttagga gtcactttga aatacctggg ccataagcaa agtggcattt
     9661 tcacctttca ggagaaactg gtacacttat ccattctata gtgcatgctt gttcaattgg
     9721 gctgatgact aaaccggtga ctaaaggttt gtcagtataa atggatgggt tgtaggcaga
     9781 cggtgaggaa ttactatacc tgcaaggagt cattgcctga tctgcctgga aaggggcagg
     9841 attgagtctc agaaggtgta caccatagga tatggaaaaa tttgtcacgc ctagcattca
     9901 acttagtggt gtagcgccac ctactggcac tttaaaagct tagcatagag gagcatgtgt
     9961 gttaggagct cggatgggat ccagggcctc aaggtttgca tgtaaataaa agccctttac
    10021 caaattaact acataccagc atacatcagt cctttagtgt tgaaaaacag aagtgaaagc
    10081 taatatatat agtgcttgct ttatttaagt ctagctgatt acgtgtttgg ttgccagtgt
    10141 gactagtctg gagttgaatt tgtcctcaga cacgtaaaat ggaatttggg attcacaaca
    10201 ctctagtatg agggacctaa tggcctgtac caggcacaaa cgtgtctata aactacacaa
    10261 aacgaaggaa tttacaggaa ttaggaaggt attcttaaca ttaaaacatt atgggcattt
    10321 taaaaaaagc tttgacagga tttctttgtc atggctgtcc tggagctagt tgtgtagacc
    10381 aggctgggct gaaatcttgt ctgcctgcct ggcttggaca cttttttatt atgtatacaa
    10441 cattctgctt ccatgtatat ctgcacatta gaagacggca ccagatctcc taatggatgg
    10501 ttgtgagcca ccatgtggtt gctgggaatt gaactcagga cctctggaag agcagtgctc
    10561 ttaacctctg agccatctcc agccccagct tgggcacatt tttaatggct gggaaatcaa
    10621 accccctagg ccttctgtca gtaatgaagg gcttttggct accgagagta ggatttaagg
    10681 ttattcggag ctgcaggtct gcctcagtgc aggtttggga gtccagcatc ttagaaaatg
    10741 cagtgaagcc aagctgagct atattttgtt taaaaaaaaa ataagtgggt aaagtgctgc
    10801 tgagcctgat gaccaagctg ggacacaagt agaagaacat aggccaatgc tctatattaa
    10861 aagcatgggt catttttaat gctcttgaga aggctatgcc tacactactc tcagccaccg
    10921 cagcgtgttt aaattaaact agtttggaaa ttttctttgg gggtaagcta tttaacctag
    10981 tgccttggca ggtatactac tgaactctcc tcctcattcc tttttgtttt ttaagaattt
    11041 cagtcaggct caggcagccc ttaaacttgt gattaagcct gagaacagtt acgattatga
    11101 gcctattagt ataccgatca atatgtgaat ttttttggga tgggggtcag gcctccctgc
    11161 ctcccaaata ctgggactaa aggctgcacc accacaacct ggctcttgaa atacttttct
    11221 acattttttg gggggcatgg gtgggagagc agggtttctc tgtattagcc ctggctctcc
    11281 tggaactctg tagaccaggc tattcttgag ctcagattag cctgtctctg cctcctaaat
    11341 tctgggatta aaggtgtgtg ctactgctgc ctggctacaa agacattttt ttttttctta
    11401 aatttaaaaa caaaagtggt tcttttagaa gggtggttgg tgttggcaca tactccaagc
    11461 actcaggttt tgagtttgtc ccaggaatga agactgcatt actgccgccc ctccctggta
    11521 agggctacac agagaaatcc tatttggagc ctgtcctggt aactcgctct gtagaccagg
    11581 ctggcctcga actcaagaga accacctgcc tctgaatgct ggtattaagg gcaggcacca
    11641 ccaacaccca gcctaaaaaa tgtctttttt ttaaagattt tttttttttt tttacagaat
    11701 aaacattctg tttacaatat tctgcttcta tgtatatctg cacactagaa gagggcaccc
    11761 gatctcataa tggatggttg tgagccacca agtggttgct gggaattgaa ctcagaacct
    11821 ctggaagagc agtcagtgct cttaacctct gagccatctc tccagcccct aaaaatggct
    11881 cttgagatag ggtctcaagt agtttgagac tgagttggct atataaacaa ggctggcaca
    11941 tagcaccatg tacagctggg tttagtttac atggggtgtt tttgtctctg gaggcaggag
    12001 gatcatttga gcatagggag ttaatagtga ggtcatgttt tatctactct tctgaattga
    12061 gaactaagct gatgcaaagc aagtttgact gaagaagtcc agtttatgag aacaagggtg
    12121 gaaactaatg tgtcaaagat ggccttgcat gtgttttaga tgatgaccca gtcacttggg
    12181 aattactgga tgtgtaagac ctatatcttg acaggagtga acagtgtctt ataggtccta
    12241 tatgaaagaa atgagacata cccattttgt ttcccctaag aattcacttt tcctaacctg
    12301 gttcatgcta tttaggttat tttacttgca aatcctaggg tgctccctta cccagtattg
    12361 cttatgtggc accaaagtca ctcactccca tgatttgcaa gtctctggga acttccatga
    12421 caacctagaa tagcaactca aatacatttt ctcagtacca attttgaaga aaaaatattt
    12481 tgcaaaatag ctgtatggat gggtactaaa tagtgaggtt atctccagaa ggcctatgaa
    12541 gaattaaggt tgagttcagt tgagttcagc agcaagttta aggttcatcc atttttgtac
    12601 agtgttttcc tattacggta agtgttttgc ctgcaggaat atctgtacca catgcttgcc
    12661 tggtacctat atcggccaga agagggcttt ggatcctctg gacttgaatt acagatgggt
    12721 attagccacc atttaggtgc tgggaattga aaccaagtcc tctggaagaa cagcaagtga
    12781 ctcgacgtcc ggagctctgg cgcgcctgtg gaat
//
