LOCUS       sequence-339175-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with mutations at reported 
            dimerization interface of the DHOase domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD H1734A H1741A
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..34
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(267..290)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6958..6967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6994..7368
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(7040..7056)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(7378..7794)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(7394..7410)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7426..7448)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7853..8210
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             8273..8866
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        8896..9000
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9001..9069,9070..9861)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9219..9238)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10025..10613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10514..10533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10767..10784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             10914..11383
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(11554..11949)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(11950..11958,11959..11961,11962..12335,1..201)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12123..12142)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
       61 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
      121 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
      181 gggggggggg agggagaggg gggggggggg cggaatttac gtagcggccg cggcgcgccg
      241 gccctcgagg cctgcaggaa ttcctacttg tcgtcatcgt ctttgtagtc gaaacggccc
      301 agcacggtgg ctaacagagc catgcggatg tacatgccgt tctcagcctg gcggaagtag
      361 gctgcgcggg gatccgagtc cacttccacg cttatctcgt tgacacgggg catcgggtgc
      421 atcaccacca tcttcttctt ggcccgggtc atgatgtggg gagtgaggat gaactgacca
      481 aagcaagctt cgtactcctg ggtagagcca aatcgttcct tctggattcg agtcatgtag
      541 agcacatcag tgtcaggcag cgcctcctca atgctctcga attcctcctg cttggtgccg
      601 cgggaggcca cgaaggcccg cacagtgggt ggcatgcgca ggctgggagg tgccacgtag
      661 cgcaggctga cacgatactg ggtgagcagg caggccaggg aatgtactgt gcgtccgtgc
      721 ttcaggtcac ccaccatcgt gatcgtcatg ccattgacag ttcccagctc ctcacggatg
      781 gtgaagatgt ccagcagggc ctgggtgggg tgctctccga ccccatcccc agcattgatc
      841 actggcctcc ggcagtgctt ggcggccagc tccactgctc caggctgggg gtgccggagc
      901 acgacgacgt cggcatagca gctcatggtc tgcacggagt cagccaggga ttcgcccttc
      961 tggacggacg atgtggcttc cgagaagctg agcacagcac ctcccagccg ggccatggct
     1021 gctgcaaagg agctgctggt ccgtgtgctc acttcataga acatggaggc catgaccttc
     1081 cccttcagga tgtcgaggct ccgctccttc tgcaccatca tacgcagtgt gtgtgccaca
     1141 ttgaacaggt gagacatctg atccttggtg aactgctgga cggacaggat atgttggccc
     1201 actaatgagt gcagcagggg tgaggtctgg gggtgcagca agccaggggt ccccaggttc
     1261 tggggagatg cctgtctcgg tactggtggt ggagggtagc aggtgccatc aggggttccc
     1321 atcagctctg gctcggctac cttccgagag gacttctcct ttggctcctc agctggcaaa
     1381 cctgggtcgg aggctcgatg gattcggggc ggcagatgga agcggccatc aggaagccct
     1441 gggatgccac ggcggggtct ttcaggtgtc gtggtcatct cactagtggc aggggctgag
     1501 ggtgggagct gaggaacagc cccctgtggc cacttccgta catcctgtcc atagcccggg
     1561 ggtaccagaa cctgcccatc gatataggca acctcccctc gcaggaccac acggcggacg
     1621 gtgcccttca ctttctgccc ttcaaaaggt gtccagtggg ccttggagaa gggcatgtgg
     1681 ctgggaattg tccactcatg ctccagatcc acctccacat aggtgtcctc ctgcggaggc
     1741 agtgcaaaga tgcgccgagg atttgcgtgc aatcgctgca gcaggtcgtc caggctgagc
     1801 cggccctcgc ttacagccgt caggagtagt ggcagcatgg tctctaaccc tgggaaccca
     1861 ggtgggggcc tggacccaca cttctcctcc aaggtatggg gagcatggtc tgaggcaaag
     1921 cagtcgatga cagccatgtt ctcccacagg gcttccacat cctggcggga gccaagctca
     1981 ggccggacct cccccttccc aggccccagg cgctccaggt catcatggct taggaacagg
     2041 tggtggggag ccacctcgca ggtcactggc aagccccgtg cctttgcagc tttaattagc
     2101 aggatctcct ccttccgtgc cacgtgacat atgtgcactg agcgctgagt gagctgagcc
     2161 accatgagga cagcagccac ggtttgctgc tccgcgtgag ccacaatggg gaggtgggag
     2221 ggccatgtct cgaaatgctc catccactgg accacgctgt ccagccgcag ctcagagaag
     2281 gtctcattga ggtaaagctt cagcccggct gcagacccgg ccacggtgcc caaggttcct
     2341 gcattttcag acgaggcccc aaggaatagc gcaaagtcgc accgggcgcc agcctctgcc
     2401 agcttctggg ccagggccag agcaggggcg tcaatgatgg ggggccgggt attaggcatg
     2461 gcacacacca tggtgatacc cccagccagg gcagcggctg tgcctgaagc aaagtcctcc
     2521 ttatgtgtcc cacctggttc ccgcaggtgc acatggacat caatcaatcc cggcagtcgc
     2581 acaagctttt gggaggtcat acagtcaaca tgcaccttca aaggaggggc tggcccgatc
     2641 tggcctaggg cctccacaaa gagttttgtg cacttgatat cgatgattag gggcacggag
     2701 aagtcagcgg ccaagcgtcg ggtgcggtag cccttggtga caaaggaaga gagacgccgg
     2761 cccccagctc cacgcattga caggttaatc accagctcaa agtttttctc agctagctgc
     2821 tccaggatgc tccgctgtgg tgggcactca ccatccacag cctcctcaaa gtgccagtcc
     2881 acagctgtta ccttgacgcc atgctcagtg tagaagtcag ctgtgccgag actggcatag
     2941 aggctgtagc ccaggctctc cagtagccgc acagttggga gcagctcgct tttgttctta
     3001 tagctgccaa tggtcagcag gatattcttc ttggggatct taaagccagt gcttagcatg
     3061 gccttgaggt atgcctcaca gcggctctcc ccaaagccgg ccacctcccc agtactggtc
     3121 atttccacac ccaacaccac gtcagcaccc gccaagcggg agaaggagaa ctgaggcacc
     3181 tttactccca caactccaga accagtcatt agccccacag gttccacttc ttcccccatg
     3241 atgacccgcg tggccaaggc tactaggtcc acacccagtg tcttggaaac gaaggggaag
     3301 gagcgagaga cacgtacgtt gcattcaata actttcagct ggtcatcctt ggcaatgagc
     3361 tgcagattga agggtcctgt gacctgtagc tcctggccca cagcatgcac aatggctttg
     3421 atccgctcca gggttttggc agtgatatct tgtggggggg tcaccagcgt cgcatcacct
     3481 gaatgcacac ctgcattctc cacatgctca gagatggcga tggctgccac cacaccatca
     3541 gaggccacgg catccacgtc aatctcctta gcctcctgga tgaacttgga gatgaccacg
     3601 ggatgctctt tggagacggc tgctgcgctg ctcaggaagc gctccaggtc tccatccgtg
     3661 taggccacat tcatagcagc accgctcagc acataggagg ggcgcaccac acaggggtac
     3721 cccacggtct ggcagaattg gcgagcagac tcgaggtcac tgagctccct ccactgaggc
     3781 tggctgatac caatggtgtc aaggagccgg gaaaacttga aacggttctc agccgagtca
     3841 atggcttcag gggaggtgcc cagcacccgg cactgctgcc gatgcaacgc catggccatg
     3901 ttgttgggca gctgtccacc catggatagg atcacacctt cagggttctc gagctcatag
     3961 atgtccatca ccacctcaaa agagatctca tcaaagtaga gtcgatcaca catgtcatag
     4021 tcggtgctga ctgtctctgg gttatagttc accatgatgg tcttatatcc catctttcgg
     4081 agctgctgga tgcagcctac agcacaccag tcaaattcaa cgctagagcc aatacggtag
     4141 acgccagagc caaggactag gacatgaggt gttcgaaagg tgaggtcatg ggtggtgccc
     4201 caatacgtta ggtataggta atttgtctgg gctggccact cagctgcaac tgtgtcaatc
     4261 tgtttcactg ctggacagat ccccagttcc tgacgcagct tgcgaacagc cagctctgtg
     4321 ctcagaactg caagggcaat ctgtttgtct gagaagccaa gacacttggc ctgttgcagc
     4381 aggtctggcg gcaaaggctg tccacggtgt tgttctagca gctgggcatg tgcgatgata
     4441 cgcttcattc ggtgcaggaa ccagcggtcg atgcgtgtga gctcatacag gcggtccact
     4501 gaataaccag cccacaaagc agctgccacc acaaaaatcc gcttatctgt tggagtctcc
     4561 aactccatat cgctgactgg tttcactgtg tgatcaaagc ccacacagtt ctcatccacc
     4621 atgcgcaggg ccttctggaa ggcctcctca aatgaacgcc caatgcccat gacttcacca
     4681 acgctcttca tgcagctccc aatctttgtg ctgactcgca ggaacttgct aaggtcccat
     4741 cgaggaatct tcaccacaca ataatccacg ctgggttcaa aggctgctgt accccctgtc
     4801 acagagttcc tgagctcagg caaagggatg cccaatgcta gcttggctgc cacataagcc
     4861 agtggataac ctgtggcctt actggccagg gcagagctgc gagagagcct ggcattcact
     4921 tcaatgatgt aatactgctc agactcaggg ttcaaggcat actgcacatt gcactcccca
     4981 acaattccca ggtgctgggt caccttgata gctgtctgcc tcaggagctg atactccctg
     5041 tcattcagtg tctggctagg ggccaccact atggactcac cagtgtggat gcccagtggg
     5101 tccaagttct ccatgttaca caccgtgaca cagttgccat aggcgtctct caccacctcg
     5161 tactcaatct ccttccatcc cttcagagac ttgtctacta gcacttggct ggtatgggca
     5221 aaagctgggg ccacgagagc agagagctcc tccctgttag aggcaaagcc agagcccagg
     5281 ccacccaggg caaaggctgc acgcactagc acagggtacc ccagccgttc agcggctgcc
     5341 tgggcctgtt caagagaatt tgctgcctcg ctcggggcca catgctctcc gatctctgcc
     5401 attctggcag caaaggcccg tcgatcctcg gtcagctcaa tggtctccac tggtgtgccc
     5461 aggacccgga ccccataccg agccagcacc ccggccttgg tcagctccac accacagttc
     5521 agagcagtct ggcccccaaa agtcagtaac acaccatcgg ggcgttcatt acgtatcacc
     5581 tgggttacat aatgaggtgt tatgggaaga aaatagacct tgtcggccag cccctgggag
     5641 gtctgcactg tggcaatatt ggggttgatc agcaacgtct ggatgttttc ctccttcagg
     5701 gccttaattg cctgagagcc cgagtagtca aattctccag cttggccaat ggagaggccc
     5761 cctgagccca ggatcagaac ctttcgtggt ggtggaagtc cagagccggg agtgggaatc
     5821 ccagggggac agaggcgctc agtcagccgc tctctaactg tctggccccc agggttccca
     5881 gctgtggcct ctttcacagt ttccagaaag atatcgaaaa gcagttccat atctgaaggg
     5941 ccagcttggt gctctgggtg aaactggaca ctgaagaaag gcaagctgtt gtgcacaatg
     6001 ccttcattgg aaccatcatt ggcgttggtg aagagaggag cccagtctgc tggcagtgag
     6061 tctgtctcca cagcaaaccc atggttctgg gatgtcagaa agcagcgccc agagcccacc
     6121 aacaagcagg gctggttatg gcctcggttc ccatatctca tcttgtaagt cttggcccca
     6181 atggctaagg ccaatagctg gtgtcccagg cagatcccaa agacaggtcg gggattaggc
     6241 tcagataaaa cacggctcag tgtggatacg acactgggat aggaggcagg gtcaccaggc
     6301 ccattactta agaagagacc ctcatactct tggctgtcta gtgcatggtc ccagggtacc
     6361 acagtgacct cagccccacg ctggcagagg catcggatct gattatactt gaggccacag
     6421 tccaaagcaa ggatccgagg ggcaccccct gtattgaata cccgtggagt cttaatggag
     6481 acctctggta ccagggggcg ggcattgggg tccaagaatg gcagacttga aggttctgtt
     6541 ccattctgga ccagcttccc cagcagagac ccctgttccc gcaacttctt ggtcagctcc
     6601 cgagtgtcta ctccttgcaa gccagggatg ccatgctgct gcagccactc atgcagggtg
     6661 cgggtggcac tccagtggct gggagtagga cagcactctc ccactaccag tgctgctacg
     6721 tggatgcccg aggattcaaa ccacttgcag agaccgaact catccatttc atctgggggg
     6781 atgccatagt tgccgatcag aggataggtg agcactaaga tctgtgcctt gtaggaggga
     6841 tcagtgaggg cctcggggta gccgaccatg ccggtttgaa acaccacttc cccggcagtc
     6901 gacacggcgg ccccaaaggg ctggccccgc aggaccgacc cgtcctccaa cactagggcc
     6961 gccatggatc cttaattaac tagctagatc cggcagtcta gaggatggtc cacccccggg
     7021 gtcggcagcc ttcacgtggg cggcgtgtat ccaagctgcg atgccgtcta ctttgagggc
     7081 ggtgggggtg gtcagcagga ctgtgtaagg tcctttccag cgaggttcta ggttcttagt
     7141 ctggtgtcgg cggacccaca ctgtgtcgcc gactcggtaa gggtgaggta ccaccggtcg
     7201 gtccagttgt tcttggtagg ctgccgccag aggtctccag acttcgtgct ggactaagta
     7261 gagagcctgt aagtgagctt ggagagaggg gctgttagta actcttgtca tgtcagggtc
     7321 agggaagttt acaaggggcg ggggtgcccc atataagatc tcatatggcc atatgggggc
     7381 gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg
     7441 ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag ggtgtacaaa
     7501 gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaggggac
     7561 ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct gggtgatgag
     7621 gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc attctgcaga
     7681 gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag cgatccgctc
     7741 gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct ggccctaatt
     7801 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcagac
     7861 aagacgcgcg gcgcggcttc ggtcccaaac cgaaagcaaa aattcagacg gaggcgggaa
     7921 ctgttttagg ttctcgtctc ctaccagaac cacatatccc tcctattagg ggggtgcacc
     7981 aaagagtcca aaacgatcgg gatttttgga ctcaggtcgg gccacaaaaa cggcccccga
     8041 agtccctggg acgtctccca gggttgcggc cgggtgttcc gaactcgtca gttccaccac
     8101 gggtccgcca gatacagagc tagttagcta actagtaccg acgcaggcgc ataaaatcag
     8161 tcatagacac tagacaatcg gacagacaca gataagttgc tggccagctt acctcccggt
     8221 ggtgggtcgg tggtccctgg gcaggggtct cccgatcccg gacgagcccc caaatgaaag
     8281 acccccgctg acgggtagtc aatcactcag aggagaccct cccaaggaac agcgagacca
     8341 caagtcggat gcaactgcaa gagggtttat tggatacacg ggtacccggg cgactcagtc
     8401 aatcggagga ctggcgcgcc gagtgagggg ttgtgggctc ttttattgag ctcggggagc
     8461 agaagcgcgc gaacagaagc gagaagcgaa ctgattggtt agttcaaata aggcacaggg
     8521 tcatttcagg tccttggggc accctggaaa catctgatgg ttctctagaa actgctgagg
     8581 gctggaccgc atctggggac catctgttct tggccctgag ccggggcagg aactgcttac
     8641 cacagatatc ctgtttggcc catattcagc tgttccatct gttcttggcc ctgagccggg
     8701 gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttcc
     8761 tgaccttgat ctgaactttt ctattctcag ttatgtattt ttccatgcct tgcaaaatgg
     8821 cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcattatgc aggtggcact
     8881 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     8941 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9001 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9061 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     9481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     9541 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     9601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     9661 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     9721 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     9781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     9841 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9901 ttgcggccgg ccgcaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10021 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10081 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10141 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10201 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10261 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10381 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10561 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10621 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10681 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10741 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10801 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaac tatggccatt
    10861 taatgtaaat acttaagaaa aaaaaccaaa ttaattttga tacatgctgc atgtgaaaga
    10921 cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac
    10981 aagtcggatg caactgcaag agggtttatt ggatacacgg gtacccgggc gactcagtca
    11041 atcggaggac tggcgccccg agtgaggggt tgtgggctct tttattgagc tcggggagca
    11101 gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt
    11161 catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg
    11221 ctggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc
    11281 acagatatcc tgtttggccc atattcagct gttccatctg ttcttggccc tgagccgggg
    11341 caggaactgc ttaccacaga tatcctgttt ggcccatatt cagctgttcc atctgttcct
    11401 gaccttgatc tgaacttctc tattctcagt tatgtatttt tccatgcctt gcaaaatggc
    11461 gttacttaag ctagcttgcc aaacctacag gtggggtctt tcattccccc ctttttctgg
    11521 agactaaata aaatctttta ttttatcgtc gacttagccc tcccacacat aaccagaggg
    11581 cagcaattca cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt
    11641 gatcccagga tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc
    11701 cctgttctca tttccgatcg cgacgataca agtcaggttg ccagctgccg cagcagcagc
    11761 agtgcccagc accacgagtt ctgcacaagg tcccccagta aaatgatata cattgacacc
    11821 agtgaagatg cggccgtcgc tagagagagc tgcgctggcg acgctgtagt cttcagagat
    11881 ggggatgctg ttgattgtag ccgttgctct ttcaatgagg gtggattctt cttgagacaa
    11941 aggaaccatg gttgtggcca tattatcatc gtgtttttca aaggaaaacc acgtccccgt
    12001 ggttcggggg gcctagacgt ttttttaacc tcgactaaac acatgtaaag catgtgcacc
    12061 gaggccccag atcagatccc atacaatggg gtaccttctg ggcatccttc agccccttgt
    12121 tgaatacgct tgaggagagc catttgactc tttccacaac tatccaactc acaacgtggc
    12181 actggggttg tgccgccttt gcaggtgtat cttatacacg tggcttttgg ccgcagaggc
    12241 acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt cgctacagac gttgtttgtc
    12301 ttcaagaagc ttccagagga actgcttcct tcacg
//
