LOCUS       sequence-339176-       12272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged CAD with deletion of amino acids 
            R1398 - S1407 and F1366 - P1376 in allosteric domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12272)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2
FEATURES             Location/Qualifiers
     source          1..12272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..48
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    74..448
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     386..402
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      475..484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7089..7112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7178..7752,7753..7755,7756..7764)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7345..7362)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7572..7591
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7765..8160
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8331..8800
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8930..8947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9101..9689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9181..9200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9853..10644,10645..10713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10476..10495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10714..10818)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             10848..11441
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    11504..11861
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             join(11920..12272,1..64)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     join(12266..12272,1..16)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
       61 cgcccccata tggccatatg agatcttata tggggcaccc ccgccccttg taaacttccc
      121 tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact tacaggctct
      181 ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag aacaactgga
      241 ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc gccgacacca
      301 gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga ccacccccac
      361 cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga aggctgccga
      421 ccccgggggt ggaccatcct ctagactgcc ggatctagct agttaattaa ggatccatgg
      481 cggccctagt gttggaggac gggtcggtcc tgcggggcca gccctttggg gccgccgtgt
      541 cgactgccgg ggaagtggtg tttcaaaccg gcatggtcgg ctaccccgag gccctcactg
      601 atccctccta caaggcacag atcttagtgc tcacctatcc tctgatcggc aactatggca
      661 tccccccaga tgaaatggat gagttcggtc tctgcaagtg gtttgaatcc tcgggcatcc
      721 acgtagcagc actggtagtg ggagagtgct gtcctactcc cagccactgg agtgccaccc
      781 gcaccctgca tgagtggctg cagcagcatg gcatccctgg cttgcaagga gtagacactc
      841 gggagctgac caagaagttg cgggaacagg ggtctctgct ggggaagctg gtccagaatg
      901 gaacagaacc ttcaagtctg ccattcttgg accccaatgc ccgccccctg gtaccagagg
      961 tctccattaa gactccacgg gtattcaata cagggggtgc ccctcggatc cttgctttgg
     1021 actgtggcct caagtataat cagatccgat gcctctgcca gcgtggggct gaggtcactg
     1081 tggtaccctg ggaccatgca ctagacagcc aagagtatga gggtctcttc ttaagtaatg
     1141 ggcctggtga ccctgcctcc tatcccagtg tcgtatccac actgagccgt gttttatctg
     1201 agcctaatcc ccgacctgtc tttgggatct gcctgggaca ccagctattg gccttagcca
     1261 ttggggccaa gacttacaag atgagatatg ggaaccgagg ccataaccag ccctgcttgt
     1321 tggtgggctc tgggcgctgc tttctgacat cccagaacca tgggtttgct gtggagacag
     1381 actcactgcc agcagactgg gctcctctct tcaccaacgc caatgatggt tccaatgaag
     1441 gcattgtgca caacagcttg cctttcttca gtgtccagtt tcacccagag caccaagctg
     1501 gcccttcaga tatggaactg cttttcgata tctttctgga aactgtgaaa gaggccacag
     1561 ctgggaaccc tgggggccag acagttagag agcggctgac tgagcgcctc tgtccccctg
     1621 ggattcccac tcccggctct ggacttccac caccacgaaa ggttctgatc ctgggctcag
     1681 ggggcctctc cattggccaa gctggagaat ttgactactc gggctctcag gcaattaagg
     1741 ccctgaagga ggaaaacatc cagacgttgc tgatcaaccc caatattgcc acagtgcaga
     1801 cctcccaggg gctggccgac aaggtctatt ttcttcccat aacacctcat tatgtaaccc
     1861 aggtgatacg taatgaacgc cccgatggtg tgttactgac ttttgggggc cagactgctc
     1921 tgaactgtgg tgtggagctg accaaggccg gggtgctggc tcggtatggg gtccgggtcc
     1981 tgggcacacc agtggagacc attgagctga ccgaggatcg acgggccttt gctgccagaa
     2041 tggcagagat cggagagcat gtggccccga gcgaggcagc aaattctctt gaacaggccc
     2101 aggcagccgc tgaacggctg gggtaccctg tgctagtgcg tgcagccttt gccctgggtg
     2161 gcctgggctc tggctttgcc tctaacaggg aggagctctc tgctctcgtg gccccagctt
     2221 ttgcccatac cagccaagtg ctagtagaca agtctctgaa gggatggaag gagattgagt
     2281 acgaggtggt gagagacgcc tatggcaact gtgtcacggt gtgtaacatg gagaacttgg
     2341 acccactggg catccacact ggtgagtcca tagtggtggc ccctagccag acactgaatg
     2401 acagggagta tcagctcctg aggcagacag ctatcaaggt gacccagcac ctgggaattg
     2461 ttggggagtg caatgtgcag tatgccttga accctgagtc tgagcagtat tacatcattg
     2521 aagtgaatgc caggctctct cgcagctctg ccctggccag taaggccaca ggttatccac
     2581 tggcttatgt ggcagccaag ctagcattgg gcatcccttt gcctgagctc aggaactctg
     2641 tgacaggggg tacagcagcc tttgaaccca gcgtggatta ttgtgtggtg aagattcctc
     2701 gatgggacct tagcaagttc ctgcgagtca gcacaaagat tgggagctgc atgaagagcg
     2761 ttggtgaagt catgggcatt gggcgttcat ttgaggaggc cttccagaag gccctgcgca
     2821 tggtggatga gaactgtgtg ggctttgatc acacagtgaa accagtcagc gatatggagt
     2881 tggagactcc aacagataag cggatttttg tggtggcagc tgctttgtgg gctggttatt
     2941 cagtggaccg cctgtatgag ctcacacgca tcgaccgctg gttcctgcac cgaatgaagc
     3001 gtatcatcgc acatgcccag ctgctagaac aacaccgtgg acagcctttg ccgccagacc
     3061 tgctgcaaca ggccaagtgt cttggcttct cagacaaaca gattgccctt gcagttctga
     3121 gcacagagct ggctgttcgc aagctgcgtc aggaactggg gatctgtcca gcagtgaaac
     3181 agattgacac agttgcagct gagtggccag cccagacaaa ttacctatac ctaacgtatt
     3241 ggggcaccac ccatgacctc acctttcgaa cacctcatgt cctagtcctt ggctctggcg
     3301 tctaccgtat tggctctagc gttgaatttg actggtgtgc tgtaggctgc atccagcagc
     3361 tccgaaagat gggatataag accatcatgg tgaactataa cccagagaca gtcagcaccg
     3421 actatgacat gtgtgatcga ctctactttg atgagatctc ttttgaggtg gtgatggaca
     3481 tctatgagct cgagaaccct gaaggtgtga tcctatccat gggtggacag ctgcccaaca
     3541 acatggccat ggcgttgcat cggcagcagt gccgggtgct gggcacctcc cctgaagcca
     3601 ttgactcggc tgagaaccgt ttcaagtttt cccggctcct tgacaccatt ggtatcagcc
     3661 agcctcagtg gagggagctc agtgacctcg agtctgctcg ccaattctgc cagaccgtgg
     3721 ggtacccctg tgtggtgcgc ccctcctatg tgctgagcgg tgctgctatg aatgtggcct
     3781 acacggatgg agacctggag cgcttcctga gcagcgcagc agccgtctcc aaagagcatc
     3841 ccgtggtcat ctccaagttc atccaggagg ctaaggagat tgacgtggat gccgtggcct
     3901 ctgatggtgt ggtggcagcc atcgccatct ctgagcatgt ggagaatgca ggtgtgcatt
     3961 caggtgatgc gacgctggtg acccccccac aagatatcac tgccaaaacc ctggagcgga
     4021 tcaaagccat tgtgcatgct gtgggccagg agctacaggt cacaggaccc ttcaatctgc
     4081 agctcattgc caaggatgac cagctgaaag ttattgaatg caacgtacgt gtctctcgct
     4141 ccttcccctt cgtttccaag acactgggtg tggacctagt agccttggcc acgcgggtca
     4201 tcatggggga agaagtggaa cctgtggggc taatgactgg ttctggagtt gtgggagtaa
     4261 aggtgcctca gttctccttc tcccgcttgg cgggtgctga cgtggtgttg ggtgtggaaa
     4321 tgaccagtac tggggaggtg gccggctttg gggagagccg ctgtgaggca tacctcaagg
     4381 ccatgctaag cactggcttt aagatcccca agaagaatat cctgctgacc attggcagct
     4441 ataagaacaa aagcgagctg ctcccaactg tgcggctact ggagagcctg ggctacagcc
     4501 tctatgccag tctcggcaca gctgacttct acactgagca tggcgtcaag gtaacagctg
     4561 tggactggca ccagcggagc atcctggagc agctagctga gaaaaacttt gagctggtga
     4621 ttaacctgtc aatgtttgtc accaagggct accgcacccg acgcttggcc gctgacttct
     4681 ccgtgcccct aatcatcgat atcaagtgca ccaaactctt tgtggaggcc ctaggccaga
     4741 tcgggccagc ccctcctttg aaggtgcatg ttgactgtat gacctcccaa aagcttgtgc
     4801 gactgccggg attgattgat gtccatgtgc acctgcggga accaggtggg acacataagg
     4861 aggactttgc ttcaggcaca gccgctgccc tggctggggg tatcaccatg gtgtgtgcca
     4921 tgcctaatac ccggcccccc atcattgacg cccctgctct ggccctggcc cagaagctgg
     4981 cagaggctgg cgcccggtgc gactttgcgc tattccttgg ggcctcgtct gaaaatgcag
     5041 gaaccttggg caccgtggcc gggtctgcag ccgggctgaa gctttacctc aatgagacct
     5101 tctctgagct gcggctggac agcgtggtcc agtggatgga gcatttcgag acatggccct
     5161 cccacctccc cattgtggct cacgcggagc agcaaaccgt ggctgctgtc ctcatggtgg
     5221 ctcagctcac tcagcgctca gtgcacatat gtcacgtggc acggaaggag gagatcctgc
     5281 taattaaagc tgcaaaggca cggggcttgc cagtgacctg cgaggtggct ccccaccacc
     5341 tgttcctaag ccatgatgac ctggagcgcc tggggcctgg gaagggggag gtccggcctg
     5401 agcttggctc ccgccaggat gtggaagccc tgtgggagaa catggctgtc atcgactgct
     5461 ttgcctcaga ccatgctccc cataccttgg aggagaagtg tgggtccagg cccccacctg
     5521 ggttcccagg gttagagacc atgctgccac tactcctgac ggctgtaagc gagggccggc
     5581 tcagcctgga cgacctgctg cagcgattgc accacaatcc tcggcgcatc tttcacctgc
     5641 ctccgcagga ggacacctat gtggaggtgg atctggagca tgagtggaca attcccagcc
     5701 acatgccctt ctccaaggcc cactggacac cttttgaagg gcagaaagtg aagggcaccg
     5761 tccgccgtgt ggtcctgcga ggggaggttg cctatatcga tgggcaggtt ctggtacccc
     5821 cgggctatgg acaggatgta cggaagtggc cacagggggc tgttcctcag ctcccaccct
     5881 cagcccctgc cactagtgag atgaccacga cacctgaaag accccgccgt ggcatcccag
     5941 ggcttcctga tggccgcttc catctgccgc cccgaatcca tcgagcctcc gacccaggtt
     6001 tgccagctga ggagccaaag gagaagtcct ctcggaaggt agccgagcca gagctgatgg
     6061 gaacccctga tggcacctgc taccctccac caccagtacc gagacaggca tctccccaga
     6121 acctggggac ccctggcttg ctgcaccccc agacctcacc cctgctgcac tcattagtgg
     6181 gccaacatat cctgtccgtc cagcagttca ccaaggatca gatgtctcac ctgttcaatg
     6241 tggcacacac actgcgtatg atggtgcaga aggagcggag cctcgacatc ctgaagggga
     6301 aggtcatggc ctccatgttc tatgaagtga gcacacggac cagcagctcc tttgcagcag
     6361 ccatggcccg gctgggaggt gctgtgctca gcttctcgga agccacatcg tccgtccaga
     6421 agggcgaatc cctggctgac tccgtgcaga ccatgagctg ctatgccgac gtcgtcgtgc
     6481 tccggcaccc ccagcctgga gcagtggagc tggccgccaa gcactgccgg aggccagtga
     6541 tcaatgctgg ggatggggtc ggagagcacc ccacccaggc cctgctggac atcttcacca
     6601 tccgtgagga gctgggaact gtcaatggca tgacgatcac gatggtgggt gacctgaagc
     6661 acggacgcac agtacattcc ctggcctgcc tgctcaccca gtatcgtgtc agcctgcgct
     6721 acgtggcacc tcccagcctg cgcatgccac ccactgtgcg ggccttcgtg gcctcccgcg
     6781 gcaccaagca ggaggaattc gagagcattg aggaggcgct gcctgacact gatgtgctct
     6841 acatgactcg aatccagaag gaacgatttg gctctaccca ggagtacgaa gcttgctttg
     6901 gtcagttcat cctcactccc cacatcatga cccgggccaa gaagaagatg gtggtgatgc
     6961 acccgatgcc ccgtgtcaac gagataagcg tggaagtgga ctcggatccc cgcgcagcct
     7021 acttccgcca ggctgagaac ggcatgtaca tccgcatggc tctgttagcc accgtgctgg
     7081 gccgtttcga ctacaaagac gatgacgaca agtaggaatt cctgcaggcc tcgagggccg
     7141 gcgcgccgcg gccgctacgt aaattccgcc cccccccccc ctctccctcc ccccccccta
     7201 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     7261 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     7321 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     7381 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     7441 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     7501 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     7561 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     7621 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     7681 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     7741 cacgatgata atatggccac aaccatggtt cctttgtctc aagaagaatc caccctcatt
     7801 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     7861 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     7921 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     7981 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     8041 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     8101 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     8161 gtcgacgata aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc
     8221 cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac
     8281 ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg
     8341 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     8401 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     8461 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca
     8521 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     8581 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     8641 acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta
     8701 tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc
     8761 tcctctgagt gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat
     8821 taatttggtt ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg
     8881 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     8941 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     9001 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     9061 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     9121 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     9181 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     9241 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     9301 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     9361 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     9421 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     9481 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     9541 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     9601 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     9661 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     9721 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     9781 tcttcaccta gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac
     9841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcataat gaaagacccc
    10861 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca
    10921 taactgagaa tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg
    10981 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga
    11041 acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg
    11101 ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag
    11161 atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat
    11221 cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca
    11281 caacccctca ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat
    11341 ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct
    11401 cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc
    11461 gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
    11521 atctgtgtct gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact
    11581 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac
    11641 ccggccgcaa ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct
    11701 gagtccaaaa atcccgatcg ttttggactc tttggtgcac ccccctaata ggagggatat
    11761 gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt
    11821 tcggtttggg accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt
    11881 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
    11941 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
    12001 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
    12061 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
    12121 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
    12181 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
    12241 cttcctccat ccgccccgtc tctccccctt ga
//
