LOCUS       sequence-33918-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Zhang H, Fire AZ
  TITLE     Cell autonomous specification of temporal identity by Caenorhabditis
            elegans microRNA lin-4.
  JOURNAL   Dev Biol. 2010 Aug 15. 344(2):603-10.
  PUBMED    20493184
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2010 Aug 15. 344(2):603-10."
COMMENT     Sequence Label: pPD162.21
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     343..361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(439..458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     558..580
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
      301 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      361 cccttaaggc cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
      421 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      481 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      541 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      601 acgcgcgaga cgaaagggcc caagcgagga caattctcat cgttcgccta atttctttgc
      661 ttatttttta ctagttttcc ttcctcctct atatattttt atgttttctt ttaaaccttc
      721 ctccatattt ctgaattgaa tgttgttgca tattgtgtgt ccttatttca tttccatcat
      781 ccttttgtgt gtttccccct tccaccaatt ccccccacgt cctaatgaaa tgaaagcgag
      841 aagataaata aacttgcaca ttgtgttcag
//
