LOCUS       sequence-339205-       10498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Vps8 with 
            Vps8-mCherry2Bx6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Day KJ, Casler JC, Glick BS
  TITLE     Budding Yeast Has a Minimal Endomembrane System.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 
            10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
  PUBMED    29316441
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.12.014"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "56-72.e4"
COMMENT     Sequence Label: YIplac211-VPS8-mCherry2Bx6
FEATURES             Location/Qualifiers
     source          1..10498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..216
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             217..1020
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(1138..1160)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1260..1279
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1473..1495
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1487..1504
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1488..1504
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6958)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6942..6958)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6955..6977)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6966..6982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6990..6996,6997..7014,7015..7020))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8373..8394
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8511..8528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8682..9270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8762..8781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9441..10232,10233..10301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10064..10083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10302..10406)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10474..10492
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt
       61 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
      121 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
      181 cacagaacaa aaacatgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
      241 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
      301 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
      361 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
      421 tcgatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
      481 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
      541 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
      601 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
      661 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
      721 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
      781 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
      841 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
      901 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
      961 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     1021 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
     1081 ttatatcagt tattacccgc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     1141 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1201 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     1261 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     1321 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     1381 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1441 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1501 cagtgaattc gagctcatcc agggataaca ttgagtagat tgcaatctag gtgtcgtgaa
     1561 gtactttcgc ttcatgaaat atcagaaata tgcaaatggc tcctagaaag acaagtatta
     1621 ataactactg attttgatgg ctattgggtc aatcataatt ggtattctat atatgaatct
     1681 acataatgaa atgaggtgta taaattttac ttttatgtaa ccaaagttgt attaaatatt
     1741 tagaaatggc ggccgcttaa ctgctgccgc tagatccctg ggagccggag tggcggccct
     1801 cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccaact
     1861 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     1921 tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
     1981 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atagtcttct
     2041 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     2101 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     2161 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
     2221 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
     2281 aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     2341 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     2401 tgtgcacctt gaagcgcatg aactccttga tgacggactc ggtggagccg ctctcagtgc
     2461 tatccatgct agaactgctg ccgctagatc cctgggagcc ggagtggcgg ccctcggcgc
     2521 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     2581 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     2641 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     2701 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatagtc ttcttctgca
     2761 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     2821 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     2881 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     2941 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     3001 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     3061 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     3121 ccttgaagcg catgaactcc ttgatgacgg actcggtgga gccgctctca gtgctatcca
     3181 tgctagaact gctgccgcta gatccctggg agccggagtg gcggccctcg gcgcgttcgt
     3241 actgttccac gatggtgtag tcctcgttgt gggaggtgat gtccaacttg atgttgacgt
     3301 tgtaggcgcc gggcagctgc acgggcttct tggccttgta ggtggtcttg acctcagcgt
     3361 cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc agggcgccgt
     3421 cctcggggta catccgctcg gaggaggcct cccagcccat agtcttcttc tgcattacgg
     3481 ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac tcgccgtcct
     3541 gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc atcacgcgct
     3601 cccacttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg gcggggtgct
     3661 tcacgtaggc cttggagccg tacatgaact gaggggacag gatgtcccag gcgaagggca
     3721 gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct
     3781 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
     3841 agcgcatgaa ctccttgatg acggactcgg tggagccgct ctcagtgcta tccatgctag
     3901 aactgctgcc gctagatccc tgggagccgg agtggcggcc ctcggcgcgt tcgtactgtt
     3961 ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg
     4021 cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt
     4081 ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg
     4141 ggtacatccg ctcggaggag gcctcccagc ccatagtctt cttctgcatt acggggccgt
     4201 cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg tcctgcaggg
     4261 aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact
     4321 tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg tgcttcacgt
     4381 aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag ggcagggggc
     4441 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
     4501 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
     4561 tgaactcctt gatgacggac tcggtggagc cgctctcagt gctatccatg ctagaactgc
     4621 tgccgctaga tccctgggag ccggagtggc ggccctcggc gcgttcgtac tgttccacga
     4681 tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat gttgacgttg taggcgccgg
     4741 gcagctgcac gggcttcttg gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc
     4801 cgtccttcag cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca
     4861 tccgctcgga ggaggcctcc cagcccatag tcttcttctg cattacgggg ccgtcggagg
     4921 ggaagttggt gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt
     4981 cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc
     5041 cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct
     5101 tggagccgta catgaactga ggggacagga tgtcccaggc gaagggcagg gggccaccct
     5161 tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct
     5221 cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag cgcatgaact
     5281 ccttgatgac ggactcggtg gagccgctct cagtgctatc catgctagaa ctgctgccgc
     5341 tagatccctg ggagccggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
     5401 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
     5461 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
     5521 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
     5581 cggaggaggc ctcccagccc atagtcttct tctgcattac ggggccgtcg gaggggaagt
     5641 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
     5701 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     5761 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
     5821 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
     5881 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     5941 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
     6001 tgacggactc ggtggagccg ctctcagtgc tatccatgct agcgcctgaa gaaccgctgg
     6061 accccgaact tcctggatcc gttactattt ttgggttaga ttccgtctgg caaattaaac
     6121 aagaatattc atcgggtttc tgggccaagt tttcgaggca agtctggtga aagccatggt
     6181 gacatttgaa tatgacaagg ggagttttga gatctacact aatcatatct tggtggcgct
     6241 gtacattttc ccaagctaga aaaagtaatg ggtccagacc agctccccat atttttttcc
     6301 cgcagatttc gcagtcgtcg gtgtgtatag accacccttc actcagaaat tttctatact
     6361 gttgaacaag atcttgcgaa gaatcctcta taatcttttg aatcaactca gaaagagatc
     6421 tttccaattt ataagtatta aaaacattca gtagcagttg ctttaaatcc tgaactttca
     6481 ttaaaataac gtcttggtgc tcaagaacag aagacattat ttcccagaat tccccacctg
     6541 aatccttctg agaggaactc ttggacctaa ccaatcccaa aaatgcttct tgaagtagtt
     6601 tattacataa atctttcctt tcatcgtgtg aaggatattt cccatataga gtgatcagga
     6661 atgtaatcaa taatatccaa cacgattttt tctcggtacc agcagaatta caatcatcaa
     6721 aggccctcct caaagattcc aataaagtag atatatttgt tttcccttca tttagacatt
     6781 gttcaataaa acttaataaa tccctgactg ctaggttaaa actttccaag cgttcgtgaa
     6841 ttatagctgc agcttcaaaa ttagagtctt gattcaataa gtccagaatt tgtaagctct
     6901 cagcgttgct gagaactgca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     6961 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7021 gcctggggtg cctaatgagt gctgagagat cccctcataa tttccccaaa gcgtaaccat
     7081 gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg
     7141 tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat
     7201 ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg
     7261 caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg
     7321 agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga
     7381 aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa
     7441 tttcaacagg taagttagtt gctagaatcc atggctcctt tgccgacgct gagtagattt
     7501 taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc
     7561 gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc
     7621 ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt
     7681 ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca
     7741 gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg
     7801 tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg
     7861 aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg
     7921 cgactgaagc tcgcaatacc ataagccgtt tttgctcacg gatatcagac cagtcaacaa
     7981 gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga
     8041 gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt
     8101 ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt
     8161 caagtaaggc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggc
     8221 agaattggta aagagagtcg tgtaaaatat cgagttcgca cattttgttg tctgattatt
     8281 gatttttggc gaaaccattt gatcatatga caagatgtgt atctacctta acttaatgat
     8341 tttgataaaa atcattaggg gattcatcag taatgagtga gctaactcac attaattgcg
     8401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9121 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9541 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10441 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccagctt
//
