LOCUS       sequence-33924-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     Victoria Prince lab plasmids
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS ZfHoxb6a
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(635..653)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(636..653)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(671..687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(671..687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(684..706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    695..711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(719..725,726..743,744..749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    764..785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggccgcctgg ccgcgggatt cgctggaatt ggagaaggag ttccacttta acaggtatct
       61 gacccgaaga cgccgcattg agattgcgca cgcactgtgc ctgacggaac gtcagataaa
      121 gatatggttc caaaaccggc gaatgaaatg gaaaaaggag aacaaactga tcaactgttc
      181 acaaaccagt ggagaagagg aggaggagaa gaggacagaa tgaaatttta aatcataaaa
      241 tactccaaaa gaaaactatg gcatagaggg caccagactc tcttataaca caaatgaaga
      301 ctttccatca tgttagcaac tgtagcattc ctggtaactc attgtaattt cttttgtgat
      361 tctttttttt acatgtcgtt taacatgtgg ttttgtgcgc tcttactctg actttagatt
      421 tgctcttatg actgaaatgt ccatgtggaa cgaagctatt tgaaatttgt gtcatattcg
      481 tgtttatatt taagctgtgt aagaataaaa aattcaattg tacggaaaaa aaaaaaaaaa
      541 aagcttgagc tcgagtcctc aatcactagt gcggccgcct gcaggtcgac catatgggag
      601 agctcccaac gcgttggatg catagcttga gtattctata gtgtcaccta aatagcttgg
      661 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      721 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      781 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgcactgcat
      841 aatgatcgca c
//
