LOCUS       sequence-339248-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UIMLx2 ubiquitin probe for K48 ubiquitylated proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Villamil M, Xiao W, Yu C, Huang L, Xu P, Kaiser P
  TITLE     The Ubiquitin Interacting Motif-Like Domain of Met4 Selectively 
            Binds K48 Polyubiquitin Chains.
  JOURNAL   Mol Cell Proteomics. 2022 Jan;21(1):100175. doi: 
            10.1016/j.mcpro.2021.100175. Epub 2021 Nov 9.
  PUBMED    34763062
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics."; date: "2022-01"; volume: "21(1):100175. doi"; pages: "
            10.1016/j.mcpro.2021.100175. Epub 2021 Nov 9"
COMMENT     Sequence Label: UIMLx2
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..51
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             58..75
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             85..102
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             106..399
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGISIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 atgggcggtc tgaacgacat cttcgaggct cagaaaatcg aatggcacga aagcagccat
       61 catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatgtc ggactcagaa
      121 gtcaatcaag aagctaagcc agaggtcaag ccagaagtca agcctgagac tcacatcaat
      181 ttaaaggtgt ccgatggatc ttcagagatc ttcttcaaga tcaaaaagac cactccttta
      241 agaaggctga tggaagcgtt cgctaaaaga cagggtaagg aaatggactc cttaagattc
      301 ttgtacgacg gtattagcat tcaagctgat cagacccctg aagatttgga catggaggat
      361 aacgatatta ttgaggctca cagagaacag attggtggat ccgaattcga gctccgtcga
      421 caaggcaaca ctaacctata tgaccacgct gtcacgccag aaatactctt agaacagttg
      481 gcctacgtgg ataacttcat accatctctg gataacgagt tctctaatgt ggattggggc
      541 ggcggcggca acactaacct atatgaccac gctgtcacgc cagaaatact cttagaacag
      601 ttggcctacg tggataactt cataccatct ctggataacg agttctctaa tgtggattgg
      661 tga
//
