LOCUS       sequence-339263-        6627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes fly DGCR8 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6627)
  TITLE     Zeng Lab Plasmids
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKMyc-fly DGCR8
FEATURES             Location/Qualifiers
     source          1..6627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..26
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    143..164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(179..184,185..202,203..209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     222..244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     241..257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     241..257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        286..665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        666..869
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     820..840
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     866..890
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1100..1118
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1100..1117
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1131..1150
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1205..1234
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      3199..3208
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3212..3346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3249..3268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3303..3322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        3415..3772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3415..3606
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3438..3458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3623..3758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3792..3809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3792..3808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3801..3823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4021..4476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4108..4127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4318..4339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4493..4512)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4612..4634
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4672..4690)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4758..4862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4863..4931,4932..5723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5081..5100)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5894..6482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6383..6402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
       61 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      241 caggaaacag ctatgacatg attacgaatt aattcgagct cgcccgacat tgattattga
      301 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      661 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      721 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      781 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      841 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      901 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      961 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
     1021 gagtctatag gcccaccccc ttggcttcgt tagaacgcgg ctacaattaa tacataacct
     1081 tatgtatcat acacatacga tttaggtgac actatagaat aacatccact ttgcctttct
     1141 ctccacaggt gtccactccc aggtccaact gcacctcggt tctaagcttc tgcaggtcga
     1201 catggaacag aaactcatct ctgaagagga tctgtctaga ggatccatgg cggagaagcc
     1261 gctggccgac tgcccggact ctgggcaggt ccgcaagcgt ccccgtctgg aggacgagga
     1321 gcaggagctc aaggtggccg gcttttcgct gagcgccatc gaggaaatgc ggcggaccag
     1381 ggacaacgag ctgcagaacg aacccatgga ccagcaactg cgacagttcc aggttctcga
     1441 cgaagtcggc tctggcagcg atgaggatga tgagtcagac aacgctagcg agcagcaggg
     1501 cggaaggtat ggagaaggag gcgacgagga ggaagaggcg gctgatggtg acggcgaaga
     1561 atacgattcc tcggaattcg atgatgacga gatcgagaat ctgctggacg agaagctgcc
     1621 cgaagagctc cgcgagtcaa agcagcccaa atacgagcag cgtttcaaga ctgtgctgga
     1681 agaaaagcgc ctcaaccact ttgaagtcct acccgaggga tgggtgcagg tgacccacaa
     1741 cagtggaatg ccgctcttct tgcatcgcaa aactcgcgtc tgttgcgcat cgaggcccta
     1801 ttttctcggc actggaagtg cccgcaagca tgcagtgccc ttgggagcaa tcccctgtct
     1861 taactacagg cgggctttgg aggaagagtc ggaggtggag caacaaaaga cggatggagc
     1921 gccgccagtg gctgtgtgtc ccatggccgg agcttccgct ttaccggccg aagccatgga
     1981 cgtggaggga aaagagaaca acgaaggcag caccgattcc aaaacccaac cagatggaat
     2041 aactgccctt atgccggctg ctaaaattgt tacggtcaac gagaataccc aaaaggaatc
     2101 cattacaccg gagcaactaa acacctactg ccagaaactc ttcaaattca aggtgattcg
     2161 tgtgctgcga tttcggagtt ggaatgctcg ccgcaagttc acaaaaaacc gcaaacacat
     2221 caagaatatt caacgaccca cgctgccgga tggcactaag cttattaagt tcccaatact
     2281 agcgaatgct ggcgaaggat caacgaaccc gcgcggtcgg aaggagtgga tcatgaatcc
     2341 caatggcaag agcttcgtct gcatcctgca cgagtacgtg cagcatgctc tgaaaacgca
     2401 gcccacctat gagttcaagg agctccaaaa cgcagccacg ccttactccg ccaccgtatc
     2461 ggtgaacaat ctcaagtatg gcacgggcta tggcactagt aaaaagcagg ccaagtcaga
     2521 ggcggctcgg gaaacgctgg aaatactcat tcccgatgtc aaggacaaga taacgggcaa
     2581 caataaggac caaaagagcg gcactgcgaa gaaagcgcag agcgatctgt ctgttttcga
     2641 tgacatccgc atcgaagatc cccgagtcac cgaattctgc aacaagacga cggagccatc
     2701 tcccaacgcc attctgctga cttgcttgca gcgcaactac ggcagcgatg ttcagatcag
     2761 ccaggagatc aaccgcaccg cgaataacaa gaacgagttt accatgaccg tgggcaaaca
     2821 tactgccaaa gtggtgtgta aaaacaagcg ggagggcaag cagttggctt cgcaagcaat
     2881 cctccagatt ttgcatccgc acatccagac ctggggttcc ttgttgcgcc tctacggcaa
     2941 caactccata aagaccttca aggagaagaa gctggaggag caggagatca ccgtgctgca
     3001 gagcaaggcg gcagtcaacc aacccaacta cgccatactg gacaaactta aatccgagat
     3061 gttgaaactg tgtgccaagc aagagagcgt cctaaccatg ggcacttttg tgccgccaag
     3121 cgacgtcgat ctgcccactt cctccgggtc caatttgaac aacgtggaac tttgagcggc
     3181 cgcttgaatt catcgatggc cgccatggcc caacttgttt attgcagctt ataatggtta
     3241 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3301 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg
     3361 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     3421 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctgtt
     3781 aacagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     3841 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     3901 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     3961 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt
     4021 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4081 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4141 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4201 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     4261 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4321 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     4381 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4441 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct
     4501 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     4561 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4621 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4681 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4741 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4801 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4861 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4921 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4981 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5041 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5101 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5161 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5221 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5281 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5341 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5401 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5461 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5521 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5581 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5641 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5701 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5761 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5821 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5881 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5941 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6001 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6061 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6121 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6181 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6241 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6301 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6361 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6421 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6481 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6541 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6601 ctgataccgc tcgccgcagc cgaacga
//
