LOCUS       sequence-339265-        3462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a human miR-122-expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Li P, Chen Y, Juma CA, Yang C, Huang J, Zhang X, Zeng Y
  TITLE     Differential Inhibition of Target Gene Expression by Human 
            microRNAs.
  JOURNAL   Cells. 2019 Jul 30;8(8). pii: cells8080791. doi: 
            10.3390/cells8080791.
  PUBMED    31366019
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2019-07-30"; volume: "8(8). pii: cells8080791. doi"; pages: "
            10.3390/cells8080791"
COMMENT     Sequence Label: pSuper-gmiR-122
FEATURES             Location/Qualifiers
     source          1..3462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(22..27,28..45,46..52)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    60..76
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     65..87
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     84..100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     84..100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     119..139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        121..139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     176..192
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     176..192
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        212..426
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     347..366
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(622..633)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(767..786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(768..786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(796..813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(796..812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(805..827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(953..1409)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1091..1112)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1303..1322
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1436..1540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1541..1609,1610..2401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1759..1778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2572..3160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3061..3080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3314..3331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    join(3448..3462,1..7)
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
       61 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      121 aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
      181 aactagtgga tcccccgggc tgcaggaatt cgaacgctga cgtcatcaac ccgctccaag
      241 gaatcgcggg cccagtgtca ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa
      301 gatggctgtg agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct
      361 gggaaatcac cataaacgtg aaatgtcttt ggatttggga atcttataag ttctgtatga
      421 gaccacagat ctaagcttag gtcacaatat gtggagctga caaggttccc ctattatcag
      481 tgacaatggt ggaatgtgga ggtgaagtta acacctttgt ggctacagag tttccttagc
      541 agagctgtgg agtgtgacaa tggtgtttgt gtctaaacta tcaaacgcca ttatcacact
      601 aaatagctac tgctaggcaa tccttccctc gataaatgtc ttggcatcgt ttgctttgag
      661 caagaaggtt catctgatat cagtcttctc aatcttgtgt acttactgaa taaagtctgg
      721 ctcttttgca ctcgagtttt tttctagatc ccgggtaccc aattcgccct atagtgagtc
      781 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      841 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      901 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
      961 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc atttttttaa
     1021 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     1081 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     1141 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1201 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     1261 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     1321 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     1381 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     1441 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1501 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1561 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1621 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1681 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1741 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     1801 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1861 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1921 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     1981 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2041 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2101 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2161 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2221 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2281 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2341 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2401 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2461 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2521 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2581 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2641 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2701 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2761 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2821 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2881 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2941 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3001 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3061 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3121 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3181 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3241 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3301 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3361 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3421 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ct
//
