LOCUS       sequence-339280-        4691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Possesses two enzymatic activities: DNA synthesis (polymerase) and 
            exonucleolytic activity that degrades ssDNA in the 3'-5' direction 
            for proofreading purposes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4691)
  TITLE     Open Enzyme Collection
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pOpen-T5gene122
FEATURES             Location/Qualifiers
     source          1..4691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2714..2732)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2728..2775
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2780..2796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2780..2796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(2863..3451)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2943..2962)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3642..4433,4434..4502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4265..4284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4503..4607)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4623..4639
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4640..4671
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 aaaggggccg tcaatatcag ggtctctaat gaaaatcgca gtcgtcgata aagcgctgaa
       61 caacacccgt tacgacaaac acttccagct gtacggtgag gaggtcgatg tgttccacat
      121 gtgtaatgaa aaactgagcg gtcgcctgct gaaaaaacac attactattg gcacgccgga
      181 aaaccctttc gatcctaatg actacgattt cgttattctg gtgggcgcgg agcctttcct
      241 gtacttcgcc ggtaaaaaag gtatcggtga ttacaccggt aaacgcgttg aatataacgg
      301 ttacgcgaac tggatcgcct cgatctcccc ggcccagttg cacttcaaac cggaaatgaa
      361 accagtattc gatgcgaccg ttgagaacat ccatgacatc atcaacggcc gcgaaaaaat
      421 tgccaaagcc ggtgattatc gtccgattac cgatccggat gaggcggagg aatacatcaa
      481 aatggtgtac aacatggtta ttggtccggt tgcattcgac agcgaaacca gcgcgctgta
      541 ttgccgtgat ggctacctgc tgggggtgtc tatttcccat caagagtacc agggcgtgta
      601 cattgattcc gactgtctga ctgaagttgc agtttactat ctgcaaaaaa tcctggattc
      661 cgagaatcac actattgtat tccacaacct gaagttcgac atgcacttct ataaatacca
      721 cctgggtctg actttcgaca aagcacacaa agagcgtcgc ctgcatgaca ccatgctgca
      781 acattacgtc ctggatgaac gccgtgggac ccacggactg aaatctctgg ccatgaaata
      841 cacggatatg ggcgactatg actttgagct ggataagttc aaagatgact attgcaaagc
      901 ccacaaaatt aaaaaagaag atttcaccta cgatctgatc cccttcgaca ttatgtggcc
      961 ctacgctgct aaagacaccg atgctactat tcgtctgcac aacttcttcc tgccgaaaat
     1021 cgaaaaaaac gaaaaattgt gttccctgta ttacgacgtt ctgatgccag gttgtgtttt
     1081 cctgcaacgt gtcgaggacc gcggcgtacc gatctctatt gatcgtctga aagaagctca
     1141 gtaccagctg acgcacaacc tgaataaagc gcgtgaaaaa ttgtacactt acccggaggt
     1201 aaaacagctg gaacaggatc agaatgaggc gttcaacccg aactcggtaa aacaactgcg
     1261 cgtactgctg tttgattacg ttggtctgac gccgacaggc aaactcaccg acacgggcgc
     1321 agattctact gacgctgaag ctctaaacga actggccact cagcacccga ttgcgaaaac
     1381 gctgctggag atccgcaagc tgaccaagct tattagcact tatgtcgaaa aaatcctgct
     1441 gtccatcgac gccgatggtt gcatccgtac cggtttccac gaacacatga ccacttctgg
     1501 tcgtctgagc tcctctggta aattgaatct gcaacagctg ccgcgcgacg aatccatcat
     1561 taagggctgc gtagttgcac caccgggcta ccgcgtgatc gcgtgggacc tcactactgc
     1621 tgaagtatac tatgcggcag tgctgtctgg tgacagaaac atgcagcagg ttttcattaa
     1681 catgcgcaac gagccggaca agtacccgga tttccactcc aacattgcac acatggtttt
     1741 taaacttcag tgtgagccgc gtgatgtaaa aaagctgttc ccggcgctgc gtcaggcggc
     1801 caaggcaatc acctttggca ttctgtatgg ttcgggcccg gcgaaggttg cgcactctgt
     1861 caatgaagcc ctgctggaac aggcggccaa aactggtgag ccgttcgttg aatgcaccgt
     1921 ggcggatgcg aaagaataca tcgagactta cttcggtcag ttcccgcagt tgaaacgttg
     1981 gatcgacaaa tgccacgatc agattaaaaa tcacggattc atctattccc acttcggtcg
     2041 taaacgtcgc ctgcataaca tccacagcga ggaccgtggt gttcagggcg aagaaatccg
     2101 ttctggcttc aacgcaatca tccagtccgc ttccagcgat tctctccttc tgggtgccgt
     2161 tgacgctgac aacgaaatca tctcgctggg tctggagcag gaaatgaaaa ttgttatgct
     2221 ggtacacgat tccgtagtag cgattgtgcg cgaggacctg attgatcaat acaacgagat
     2281 tctgattcgc aacatccaga aggaccgtgg tatctctatc ccgggctgcc cgattggcat
     2341 tgattccgac agcgaagcag gaggtagccg cgattacagc tgtgggaaaa tgaagaaaca
     2401 gcacccgtcc attgcgtgca ttgatgatga cgaatacact cgttacgtaa aaggcgtgct
     2461 gctggatgcc gagttcgaat acaaaaaatt ggcagccatg gacaaagaac acccggatca
     2521 ctccaaatac aaagacgaca agttcattgc tgtttgcaaa gacttagata acgtgaaacg
     2581 catcctcggc gcctgaagag ctttgagacc agagttgcac ctagaatccg gcacatgtaa
     2641 aaagtcaaga atagggataa cagggtaatt ttattcccac ttaagatcgc cgctgaggtg
     2701 tcaatcgtcg gagccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     2761 ttgaggggtt ttttgcatgg tcatagctgt ttcctgagag cttggcaggt gatgacacac
     2821 attaacaaat ttcgtgagga gtctccagaa gaatgccatt aatttccata ggctccgccc
     2881 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2941 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3001 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3061 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3121 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3181 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3241 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3301 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3361 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3421 gcagattaca cgcagaaaaa aaggatctca agaaggccta ctattagcaa caacgatcct
     3481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     3541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     3601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     3661 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     3721 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     3781 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     3841 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     3901 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     3961 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4021 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4081 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4141 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     4201 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4261 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4321 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4381 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4441 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4501 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     4561 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac ctgcaccagt
     4621 cagtaaaacg acggccagta gtcaaaagcc tccgaccgga ggcttttgac ttggttcagg
     4681 tggagtggga g
//
