LOCUS       sequence-339286-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a sfGFP dropout expression cassette in place of Part 3 of 
            HexaPro-D614G spike to be used in the Spike Display system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Javanmardi K, Chou CW, Terrace CI, Annapareddy A, Kaoud TS, Guo Q, 
            Lutgens J, Zorkic H, Horton AP, Gardner EC, Nguyen G, Boutz DR, 
            Goike J, Voss WN, Kuo HC, Dalby KN, Gollihar JD, Finkelstein IJ
  TITLE     Rapid characterization of spike variants via mammalian cell surface 
            display.
  JOURNAL   Mol Cell. 2021 Dec 16;81(24):5099-5111.e8. doi: 
            10.1016/j.molcel.2021.11.024.
  PUBMED    34919820
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.11"; journalName: "Mol Cell"; date: 
            "2021-12-16- 16"; volume: "81"; issue: "24"; pages: "5099-5111.e8"
COMMENT     Sequence Label: Spike Display_Part 3 DO
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..64)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    53..277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(410..429)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    561..608
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             616..1638
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             616..1638
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    1768..1889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1805..1824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1859..1878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1938..1954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1938..1954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1951..1973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1962..1978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1986..1992,1993..2010,2011..2016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2031..2052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2169..2186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2340..2928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2420..2439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3099..3890,3891..3959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTHLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3722..3741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3960..4063)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4139..4158)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4330..4709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4710..4913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4864..4884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4915..4933
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4936..4954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4964..4988
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5107..5239
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5222..5241
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5284..5302
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     5348..5407
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     RBS             7137..7148
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             7155..7868
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDSDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      7880..7951
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7967..7994
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             9372..9437
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             9444..9467
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             9474..9497
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9540..9686
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
ORIGIN
        1 taacggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct
       61 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
      301 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
      361 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
      421 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
      481 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
      541 ggtgatggtt cacgtaccta gaagttccta ttccgaagtt cctattctct agaaagtata
      601 ggaacttcct tggccaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc
      661 gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct
      721 ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt
      781 ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa
      841 gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag
      901 ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg
      961 gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc
     1021 ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat
     1081 ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag
     1141 gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac
     1201 gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac
     1261 tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg
     1321 ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt
     1381 gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag
     1441 agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc
     1501 gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc
     1561 tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt
     1621 ccgagggcaa aggaatagca cgtactacga gatttcgatt ccaccgccgc cttctatgaa
     1681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1741 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1861 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1921 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1981 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2041 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2101 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2161 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2221 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2281 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2341 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcggaggtgg
     2401 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2461 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2521 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2581 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2641 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2701 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2761 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2821 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2881 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2941 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3001 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3061 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3121 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3181 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     3241 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3301 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3361 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3421 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3481 aggtgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3541 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3601 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3661 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3721 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3781 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3841 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3901 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3961 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4021 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcacacatt tccccgaaaa
     4081 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     4141 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     4201 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     4261 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     4321 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4381 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4441 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4501 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4561 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     4621 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4681 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4741 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4801 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4861 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     4921 tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc
     4981 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc
     5041 gatccagcct ccggactcta gcgtttaaac ttaagctgca gaagttggtc gtgaggcact
     5101 gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg
     5161 tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac
     5221 tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta aggctagagt
     5281 acttaatacg actcactata ggctagcctc gagaattcac gcgtggtacc tctagagtcg
     5341 acccgggatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     5401 cactggtgac atgttcgtgt tcctggtgct cctgcctctg gtgagcagcc agtgcgtgaa
     5461 cctgaccacc cgaacccagc tcccaccagc ctacaccaac agctttacac ggggcgtgta
     5521 ctaccctgac aaggtgttca gatctagcgt cctgcacagc actcaggacc tcttcctgcc
     5581 gttcttcagc aacgtgacat ggttccacgc catccacgtg agcggcacaa acggaaccaa
     5641 gcggtttgat aaccccgtcc tgccattcaa tgatggagtt tacttcgcca gtaccgagaa
     5701 gagtaacatc atccggggct ggatcttcgg caccaccctg gatagcaaaa cacagagcct
     5761 cctgatcgtg aacaatgcca cgaacgtcgt gatcaaggtg tgcgagttcc agttttgcaa
     5821 tgatcctttc ctgggtgtgt actaccacaa gaacaacaag agctggatgg aaagcgagtt
     5881 cagagtctac agcagcgcca acaactgcac attcgagtac gtctctcagc cttttctgat
     5941 ggaccttgag gggaaacaag gcaacttcaa gaacctgaga gaattcgtgt tcaagaacat
     6001 cgacggctac ttcaaaatct actccaagca cacacccatc aacctggtcc gggacctccc
     6061 tcagggcttc agcgccctgg aacccctggt cgacctgccc ataggcatca acataacgcg
     6121 gttccaaacc ctgctggccc tgcatagatc ctacctgact cctggcgaca gcagcagcgg
     6181 atggaccgcc ggagctgcag cctactatgt gggctacctg caacctagaa ccttcctgct
     6241 gaagtacaac gagaacggca caatcacaga cgccgtcgac tgcgccctgg accctctctc
     6301 tgagacaaag tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa
     6361 cttccgggtg cagcctacag agagcatcgt gcgatttcca aacattacca acctctgccc
     6421 cttcggcgag gtgtttaacg ccacaagatt tgcctccgtt tacgcctgga atagaaagag
     6481 aatcagcaat tgtgtggccg actactccgt gctgtataac agcgcctctt tcagcacctt
     6541 caagtgctac ggcgtttccc caacaaagct gaatgacctg tgcttcacca acgtgtacgc
     6601 cgactccttc gtaattagag gcgatgaggt gcggcagatc gcaccaggcc agaccggtaa
     6661 gatcgctgac tacaactata agctgcctga tgattttaca ggctgcgtga tcgcctggaa
     6721 ctctaacaac ctggatagca aggtgggcgg caactacaac tacctgtacc ggctgtttcg
     6781 caagtctaac ctgaaacctt tcgagagaga catctccaca gagatctacc aggccggttc
     6841 tacaccttgt aacggggtgg aaggcttcaa ctgttacttc cctctgcaaa gctacggctt
     6901 ccagcctacc aatggagtcg gctaccagcc ataccgggtg gtcgtgctgt ccttcgagtt
     6961 actccacgcc cccgccaggt ggcaggtgga aagtgaaacg tgatttcatg cgtcattttg
     7021 aacattttgt aaatcttatt taataatgtg tgcggcaatt cacatttaat ttatgaatgt
     7081 tttcttaaca tcgcggcaac tcaagaaacg gcaggttcgg atcttagcta ctagagaaag
     7141 aggagaaata ctagatgcgt aaaggcgaag agctgttcac tggtgtcgtc cctattctgg
     7201 tggaactgga tagtgatgtc aacggtcata agttttccgt gcgtggcgag ggtgaaggtg
     7261 acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa ctgccggttc
     7321 cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct cgttatccgg
     7381 accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac
     7441 gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag
     7501 gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagag gacggcaata
     7561 tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc accgccgata
     7621 aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag gatggcagcg
     7681 tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc
     7741 cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg
     7801 atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt atggatgaac
     7861 tgtacaaatg accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     7921 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     7981 tgggcctttc tgcgtttata cacctgccac cgatctacaa gaccccacct atcaaggact
     8041 tcggcggctt taactttagc cagattctcc ctgatccttc taagcctagc aagcggagcc
     8101 ctatcgagga tctgctgttc aacaaggtca ccctggccga tgccggcttt atcaaacagt
     8161 atggcgattg cctgggcgac atagccgcca gagatctgat ctgcgcccag aaattcaacg
     8221 gcctgacagt tctcccacct ctgctgaccg acgagatgat cgctcagtac acctctgccc
     8281 tgctggctgg caccatcaca tctgggtgga catttggcgc cggccccgcc ctgcagatcc
     8341 cctttcccat gcagatggcc tatagattca acggaatcgg cgtgacccag aacgtgctgt
     8401 atgaaaacca gaagctgatc gctaaccagt tcaattctgc catcggcaag atccaggact
     8461 ccctctcctc tacccccagc gccctgggca aactgcagga cgtggtgaat cagaacgccc
     8521 aagccctgaa caccctggtg aagcagctca gcagcaattt tggcgccatc agctctgtgc
     8581 tgaacgatat cctgtctaga ctggaccctc cagaagccga agtccagatc gatagactga
     8641 tcacaggcag actgcagtcc ctgcaaacct acgtgaccca acagctgatc agggccgctg
     8701 aaataagagc cagcgccaat ctcgccgcta ccaagatgtc cgagtgtgtg ctgggacagt
     8761 ctaaacgcgt tgacttctgc ggcaaaggct atcacctgat gagcttcccc cagagcgcgc
     8821 cgcacggcgt ggtgttcctg catgtgacat acgtgcctgc ccaagagaag aatttcacaa
     8881 ccgcccctgc catctgccac gacggcaagg cccacttccc aagagagggc gttttcgttt
     8941 ccaatggcac acactggttc gtgacacaaa gaaacttcta cgaaccccag attatcacca
     9001 ccgacaacac cttcgtgagt ggcaattgtg acgtggtcat cggaatcgtg aacaacacag
     9061 tgtacgaccc tctgcaacct gagctggact cttttaagga agagctggac aagtacttta
     9121 aaaaccacac cagccccgat gtggacctgg gcgacatcag tggcattaac gccagcgtgg
     9181 tgaacatcca aaaggaaatc gacagactga acgaggtggc caagaacctg aacgagtccc
     9241 tgatcgacct gcaggagctc ggcaaatacg agcagggatc cggatacatc cccgaggccc
     9301 ccagagatgg ccaggcctac gtgcggaagg acggcgagtg ggtactgctg agcacattcc
     9361 tgggcagatc cgactacaaa gaccatgacg gtgattataa agatcatgac atcgattaca
     9421 aggatgacga tgacaagagc gcctggtccc acccccagtt cgagaaggga tccctggagg
     9481 tgctgttcca gggcccaggc ggcggcggta gtggaggggg cggatctggc ggctcagctg
     9541 ctgtgggcca ggacacgcag gaggtcatcg tggtgccaca ctccttgccc tttaaggtgg
     9601 tggtgatctc agccatcctg gccctggtgg tgctcaccat catctccctt atcatcctca
     9661 tcatgctttg gcagaagaag ccacgt
//
